924 resultados para Laboratory analysis
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The search for cultural heritage preservation should be a constant, in order to minimize the lost of historical and cultural identity of a country. As part of this cultural heritage, highlights the importance of historical buildings. For the principles of restoration are met its important to know the state of conservation and the existing materials. Given the above, this work aims to study the pathological manifestations of the mortar coating from the Grupo Escolar Augusto Severo. For this, was conducted historical survey of the building, on-site visits with visual observation, collecting samples of mortar coating and characterization through visual and laboratory analysis, XRF, XRD, TG/DTG, OM and SEM-EDS. From the observations, pathological manifestations were found as: cracks, detachment of the coating, dirt, use of inappropriate material basis of cement, efflorescence, mold and mildew and also incompatible material base of cement. It was found that the pathological manifestations were intensified due to lack of coverage in some spots and especially the abandonment of the building, that many years does not have restoration or any other type of preventive and corrective maintenance. The results of laboratory tests indicated that the mortars studied are based on lime, with calcitic nature, and siliceous aggregate, with the presence of clay in two samples. From the samples collected, two are composed of lime, sand and clay and two by lime and sand. In this regard, it is important to conduct periodic inspections and maintenance, as well as carrying out restoration with use of material compatible with the original
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Both N excess and deficiency may affect cotton yield and quality. It would therefore be useful to base the N management fertilization on the monitoring of the nutritional status. This study investigated the correlations among the following determination methods of the N nutritional status of cotton (Gossypium hirsutum L., var. Latifolia): chlorophyll readings (SPAD-502 (R), Minolta), specific-ion nitrate meter (Nitrate Meter C-141, Horiba-Cardy (R)), and laboratory analysis (conventional foliar diagnosis). Samples were taken weekly from two weeks before flowering to the fifth week after the first flower. The experiment was conducted on the Fazenda Santa Tereza, Itapeva, State of São Paulo, Brazil. The crop was fertilized with 40 kg ha(-1) N at planting and 0, 30, 60, 90, and 120 kg ha(-1) of side-dressed N. The range of leaf N contents reported as adequate for samples taken 80-90 days after plant emergence (traditional foliar diagnosis) may be used as reference from the beginning of flowering when the plant is not stressed. Specific-ion nitrate meter readings can be used as a nutritional indicator of cotton nutrition from one week after pinhead until the third week of flowering. In this case, plants are well-nourished when readings exceed 8,000 mg L(-1) NO(3)(-). The chlorophyll meter can also be used to estimate the nutritional status of cotton from the third week of flowering. In this case the readings should be above 48 in well-nourished plants.
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In the industry of ceramic in Rio G. do Norte, tile stands out as the most manufactured product by this industry, being the intermittent kiln abóbada and caieira the principal type of kiln used in burning. There was a need to make a study of the influence exerted by the type of kiln in which tiles are burnt in their thermo physical properties. The analysis started with 24 raw samples of tile, which was split in two groups of 12 samples and burnt in Abóbada and Caieira kiln. Besides that, it was made study of the tax of heat transfer to the environment (for each kiln). After having been burnt the samples were taken for laboratory analysis. The properties verified were impermeability, determination of dry mass, absorption of water, the load of bending rupture and its geometric characteristics, the tests were conducted following the currents standards. The tests were carried out according to the ABNT - NBR 15310. The calculation of the rate of heat transfer showed that the abóbada kiln is more efficient than the Caieira, however the results of tests on the samples revealed no superiority of one over another sample. So the furnace had no influence on the performance of the ceramic tiles
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Urban growth of metropolitan areas has produced impacts of considerable importance on environment and water resources. Such impacts are in general associated with human activities, such as basin area uncontrolled development. In this context, Pitimbu river watershed, located at Natal metropolitan area, has been affected by uncontrolled development caused by urban expansion. Indeed, such effects have been reflected on water quantity and quality, which may produce social consequences. Pitimbu river is an important water supplier for human consumption, actually supplying a 2600 m3/h water discharge. This study aims to analyze the qualitative and quantitative aspects of water and sediment on Pitimbu river lowland portion. For this purpose, physical-chemical water properties were analyzed, and sediment macro invertebrates benthonic were monitored in two cross sections in a period between November 8th, 2007 and October 3rd, 2008. Monitoring methodology consisted of water and sediment sampling for laboratory analysis. Water quality analysis included Dissolved Oxygen, Oxygen Biochemical Demand, Nitrate, pH and Alkalinity, Suspended and Total Solids. The analysis of heavy metals in the sediment included Cadmium, Cobalt, Copper, Chrome, Silver and Nickel. Dry season water discharge data were measured and used to adjust recession function parameters, whose values reveal quick recession and strong river-aquifer unconfined interaction. Water quality analysis revealed the absence of degradation by organic composites. However, DO and BOD levels indicate that more consistent results could be provided if sampling time interval were reduced. Biomonitoring showed signs of aquatic ecosystem degradation by the absence of sensitive and abundance of resistant benthic organisms. Obtained results demonstrate the urgent need of effective management measures to provide environmental protection. The increase of environmental degradation will certainly make impracticable the use of water for human consumption
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The determination of leukocyte alkaline phosphatasd (LAP) is used as an aid to diagnose many diseases in the laboratory. For example, it can be used to distinguish chronic myeloid leukemia (CML) from other myeloproliferative disorders (particularly myelofibrosis and polycythemia) and leukemoid reactions (LR). Traditionally, this test is performed with the use of subjective cytochemical assays that assign a score to the level of LAP. Here we present a nonsubjective, quantitative, sensitive, and inexpensive chemiluminescent technique that determines LAP based on the commercial reagent Immulite (R) (AMPPD). To validate this methodology, intact leukocytes obtained from 32 healthy subjects, nine CML patients, and nine LR patients were submitted to the optimized protocol. By measuring the light emission elicited by four concentrations of neutrophils, we were able to estimate the activity of LAP per cell (the slope of the curve obtained by linear regression). A high linear correlation was found between the chemiluminescent result (slope) and the cytochemical score. The slope for healthy individuals ranged between 0.61 and 8.49 (10(-5) mV.s/cell), with a median of 2.04 (10(-5) mV.s/cell). These results were statistically different from those of CML patients (range = 0.07-1.75, median = 0.79) and LR patients (range = 3.84-47.24, median 9.58; P < 0.05).
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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O objetivo deste estudo foi o de procurar evidências da transmissão vetorial da doença de Chagas, nos domicílios e peridomicílios de indivíduos residentes em municípios da região de Botucatu, que tiveram xenodiagnóstico positivos. Foram estudados 58 indivíduos e foi coletada amostra do sangue para a realização de exames laboratoriais. Os resultados deste estudo mostraram que os indivíduos, de ambos os grupos, tinham baixa escolaridade e exerciam profissões que não exigiam qualificações técnicas. Houve discreto predomínio de indivíduos do sexo feminino. Quando comparadas às condições anteriores, verificou-se que houve discreta melhora nas condições de habitação, por outro lado, houve aumento de moradores em zona rural. Os indivíduos nascidos antes de 1983, apresentaram conhecimento e contato com triatomídeo estaticamente mais elevado quando comparado com os nascidos a partir 1983. A análise e comparação dos resultados das sorologias, referentes aos hemaglutinação passiva indireta, imunofluorescência indireta e ensaio imunoenzimático, mostrou que o ELISA apresentou maior sensibilidade. Os resultados deste estudo mostram que a população nascida a partir de 1983 não conhecia o vetor transmissor da doença de Chagas.
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This study presents new stress orientations and magnitudes from the Potiguar basin in the continental margin of Brazil. We analyzed breakout and drilled induced fractures derived from resistivity image logs run in ten oil wells. We also used direct Shmin measurements determined from hydraulic fractures and rock strength laboratory analysis. In addition, we compared these results with 19 earthquake focal mechanisms located in the crystalline basement. We observed that stress directions and magnitudes change across the basin and its basement. In the basin, the SHmax gradient of 20.0 MPa/km and the SHmax/Shmin ratio of 1.154 indicate a normal stress regime from 0.5 to 2.0 km, whereas the SHmax gradient of 24.5MPa/km and the SHmax/Shmin ratio of 1.396 indicate a strike slip stress regime from 2.5 to 4.0 km. The deeper strike-slip stress regime in the basin is similar to the regime in the basement at 1-12 km deep. This stress regime transition is consistent with an incipient tectonic inversion process in the basin. We also noted that the SHmax direction rotates from NW SE in the western part of the Potiguar basin to E W in its central and eastern part, following roughly the shoreline geometry. It indicates that local factors, as density contrast between continental and oceanic crust and sediment loading at the continental shelf influence the stress field. The concentration of fluid pressure in faults of the lowpermeability crystalline basement and its implications to establish a critically stressed fault regime in the basement is also discussed
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This dissertation includes the monitoring of coastal environmental dynamics at three points distinct from Ponta Negra beach, located on the South Coast of Natal, capital of Rio Grande do Norte, in the period June 2012 to May, 2013. For this, the following hypotheses were developed: Which actors morphodynamic and/or anthropogenic responsible for the changes in the study area? And yet, the configuration of the morphodynamic state of the beach, dissipative, reflective or intermediate? Faced with these questions , studies on the beach environment has its relevance as they may clarify the risks and responsibilities of anthropogenic intervention and also assist managers in more targeted action regarding the protection of praiais systems, since once committed, it is very difficult recover the environmental framework of the area, being greatly more feasible the development of multidisciplinary work plans that can guide human actions possible in search of an understanding to the harmonious interaction between society and the beach system. Its main goal is the understanding of the processes of coastal dynamics, methodological procedures that supported the implementation of this research were based on the object of study related literature associated with the collection of data resulting from beach profiles made monthly in spring tides (full moon), the hydrodynamic data and statistical quantification data (%) and size classification of sediment sediment after laboratory analysis. The results obtained from annual comparative tables of beach profiles, associated sedimentological analysis, indicated a positive sediment budget, tending to equilibrium for Point 01 and Point 02 negative. Have to Step 03 were added to the hydrodynamic data, which allowed also on a comparative framework, the perception of a depositional dynamics, with a tendency to decrease the accumulation of material at the end of the annual cycle. These data also allowed for the point 03, the calculation of the volume of material transported by the longshore current was around 104.280 m³/m, plus the Dean parameter which established a morphological state of the dissipative beach with specific prevalences for point 03. Thus, given the results presented in this work in a timely monitoring of coastal dynamics is expected that managers and public authorities can articulate multidisciplinary work plans, always aiming actions that seek understanding and effective commitment to the recovery of the harmonious interaction between society and Ponta Negra beach environment
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Intestinal parasitic infections are currently a source of concern for Public Health agencies in developing and developed countries. Since three ovum-and-parasite stool examinations have been demonstrated to provide sensitive results, we designed a practical and economical kit (TF-Test) that is now commercially available (Immunoassay Com. Ind. Ltda., S (a) over tildeo Paulo, Brazil). This kit allows the separate collection of three fecal specimens into a preservative solution. The specimens are then pooled, double-filtered, and concentrated by a single rapid centrifugation process. The TF-Test was evaluated in four different laboratories in a study using 1,102 outpatients and individuals living in an endemic area for enteroparasitosis. The overall sensitivity found using the TF-Test (86.2-97.8%) was significantly higher (P<0.01) than the sensitivity of conventional techniques such as the Coprotest (NIL Comercio Exterior Ltda, São Paulo, Brazil) and the combination of Lutz/Hoffman, Faust, and Rugai techniques (De Carli, Diagnostico Laboratorial das Parasitoses Humanas. Metodos e Tecnicas, 1994), which ranged from 48.3% to 75.9%. When the above combined three specimen technique was repeated with three specimens collected on different days, its sensitivity became similar (P > 0.01) to that of the TF-Test. The kappa index values of agreement for the TF-Test were consistent (P < 0.01), being higher and ranking in a better position than conventional techniques. The high sensitivity, cost/benefit ratio, and practical aspects demonstrate that the TF-Test is suitable for individual diagnosis, epidemiological inquiries, or evaluation of chemotherapy in treated communities. (C) 2004 Wiley-Liss, Inc.
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In recent years, many researchers in the field of biomedical sciences have made successful use of mathematical models to study, in a quantitative way, a multitude of phenomena such as those found in disease dynamics, control of physiological systems, optimization of drug therapy, economics of the preventive medicine and many other applications. The availability of good dynamic models have been providing means for simulation and design of novel control strategies in the context of biological events. This work concerns a particular model related to HIV infection dynamics which is used to allow a comparative evaluation of schemes for treatment of AIDS patients. The mathematical model adopted in this work was proposed by Nowak & Bangham, 1996 and describes the dynamics of viral concentration in terms of interaction with CD4 cells and the cytotoxic T lymphocytes, which are responsible for the defense of the organism. Two conceptually distinct techniques for drug therapy are analyzed: Open Loop Treatment, where a priori fixed dosage is prescribed and Closed Loop Treatment, where the doses are adjusted according to results obtained by laboratory analysis. Simulation results show that the Closed Loop Scheme can achieve improved quality of the treatment in terms of reduction in the viral load and quantity of administered drugs, but with the inconvenience related to the necessity of frequent and periodic laboratory analysis.
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The course of systemic lupus erythematosus (SLE), an autoimmune disease, is markedly affected by hormones such as estrogen and prolactin. It is well known that heavy exposure to sunlight has deleterious effects on SLE, triggering episodes of the disease. Classical explanations for this occurrence suggest that UV radiation damages DNA, which becomes immunogenic, or induces exposure of the Ro antigen in keratinocytes. In recent years, it has been shown that vitamin D3 has important effects on the immune system. Thus, we proposed an alternative hypothesis, suggesting that UV radiation, by promoting vitamin D3 synthesis, could be a factor aggravating the course of SLE after exposure to sunlight. To test this hypothesis, we injected F1(NZBxW) mice, which are prone to developing SLE, with vitamin D3, and we demonstrated a worsening of the histopathological findings in the kidney. (C) 2000 Wiley-Liss, Inc.
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This study intends to evaluate the effects of landscape physical elements (rocks and relief) associated with silviculture activities. The study was carried out on a small farm (Fazenda Santa Edwirges) covered by Eucalyptus forested situated in the Paraiba do Sul Basin, Southeast Brazil. The methodology consisted of detailed geological and geomorphological studies at 1:10,000 scale, and laboratory analysis of soil physical properties. The results showed three geologic-geomorphologic associations (ridge escarpment with granitic rocks, steep and gentle hills cut by shear zones and gentle hill with alluvial sediments) present high vulnerability for the development of the physical processes such as accelerated erosion landslides and flooding. In contrast, mountains associated with gneissic rocks present smaller vulnerability and high resilience for the development of the physical processes. The results have showed the importance of considering the interactions among landscape physical for the eucalyptus forest management contributing to a better selection area for eucalyptus cultivation and minimize adverse environment impact in road design.
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The present work showed the results of the biogas surface emissions from two large landfills. The study methodology consisted on escaping biogas analysis on the landfill surface, through the flux box and laboratory analysis. The total average emission of Bandeirantes Landfill was 0.00126 m3.CH4.m-2.h-1 and in Caieiras Landfill it was of 0.01222 m3.CH4.m-2.h-1. Those results were used to determinate the biogas escape both landfills. The total escape in Bandeirantes Landfill biogas accounted for 16% and in Caieiras, 35%. The conclusions demonstrated that there is a significant loss of biogas through the surface in both landfills, thus compromising the efficiency of the capture for using biogas.
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Ethanol with added water may be found during the process of assessing its physical and chemical properties. This addition can damage automotive vehicle engines and also may contribute to tax evasion. The present contribution describes a method based on a photothermal transparent transducer to determine the water content in ethanol. A chamber with a window of lithium tantalate coated with a thin layer of indium tin oxide was used, and a 1450-nm laser diode was employed as the excitation source. The results indicated a nearly linear response of the apparatus, as a function of the water content in water/ethanol solutions ranging from 0 to 100 (vol.%). The results for the dependency of the photothermal signal on the laser power and chopping frequency suggested that reliable results can be obtained using laser power and chopping rates above 100 mW and 10 Hz, respectively. The results reported here may be useful in the development of an alternative method that can provide real-time data on the water concentration in ethanol in a rapid, portable and unambiguous way, and that can be easily used in laboratory analyses or in gas stations. © 2013 Elsevier B.V.