Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice

The purification of primitive human hematopoietic stem cells has been impaired by the absence of repopulation assays. By using a stringent two-step strategy involving depletion of lineage-positive cells followed by fluorescence-activated cell sorting, we have purified a cell population that is highly enriched for cells capable of multilineage repopulation in nonobese diabetic/severe combined immunodeficient (NOD/SCID) recipients. These SCID-repopulating cells (SRCs) were exclusively found in a cell fraction that expressed high levels of CD34 and no CD38. Through limiting dilution analysis using Poisson statistics, we calculated a frequency of 1 SRC in 617 CD34+ CD38− cells. The highly purified SRC were capable of extensive proliferation in NOD/SCID mice. Mice transplanted with 1 SRC (at limiting cell doses) were able to produce approximately 400,000 progeny 6 weeks after the transplant. Detailed flow cytometric analysis of the marrow of highly engrafted mice demonstrated both lymphoid and myeloid differentiation, as well as the retention of a significant fraction of CD34+ CD38− cells. These highly purified fractions should be useful for identification of the cellular and molecular mechanisms that regulate primitive human hematopoietic cells. Moreover, the ability to detect and purify primitive cells provides a means to develop conditions for maintaining and/or expanding these cells during in vitro culture.

Adenoviral and adeno-associated viral transfer of genes to the peripheral nervous system

Targeted expression of foreign genes to the peripheral nervous system is interesting for many applications, including gene therapy of neuromuscular diseases, neuroanatomical studies, and elucidation of mechanisms of axonal flow. Here we describe a microneurosurgical technique for injection of replication-defective viral vectors into dorsal root ganglia (DRG). Adenovirus- and adeno-associated virus-based vectors with transcriptional competence for DRG neurons led to expression of the gene of interest throughout the first neuron of the sensory system, from the distal portions of the respective sensory nerve to the ipsilateral nucleus gracilis and cuneatus, which contains the synapses to the spinothalamic tracts. Use of Rag-1 ablated mice, which lack all B and T lymphocytes, allowed for sustained expression for periods exceeding 100 days. In immunocompetent mice, long-term (52 days) expression was achieved with similar efficiency by using adeno-associated viral vectors. DRG injection was vastly superior to intraneural injection into the sciatic nerve, which mainly transduced Schwann cells in the vicinity of the site of inoculation site but only inefficiently transduced nerve fibers, whereas i.m. injection did not lead to any significant expression of the reporter gene in nerve fibers. The versatile and efficient transduction of genes of interest should enable a wide variety of functional studies of peripheral nervous system pathophysiology.

Juris canonici : theoria et praxis ad forum tam sacramentale quàm contentiosum, tum ecclesiasticum, tum saeculare ... /

Mode of access: Internet.

Notitia conciliorum Sanctae Ecclesiae, in qua elucidantur exactissime etiam sacri canones tum veteres, novique Ecclesiae ritus, tum praecipuae partes ecclesiasticae historiae /

Mode of access: Internet.

Notitia conciliorum sanctae ecclesiae : in qua elucidantur exactissimè tùm sacri canones, tùm veteres, nouíque ecclesiae ritus, tùm praecipuae partes ecclesiasticae historiae /

Mode of access: Internet.

Notitia conciliorum Sanctae Ecclesiae : in qua elucidantur exactissime etiam sacri canones, tum veteres, novique ecclesiae ritus, tum praecipuae partes Ecclesiasticae historiae /

Mode of access: Internet.

R.P. Joannis Cabassutii ... Notitia ecclesiastica historiarum, conciliorum, et canonum ...

Mode of access: Internet.

R.P. Joannis Cabassutii ... Notitia ecclesiastica historiarum conciliorum [et] canonum in vicem collatorum ... ecclesiae rituum ab ipsis Ecclesia christiana incunabulis ...

Mode of access: Internet.

School of Nursing Class of 1998

Top Row: Kathleen S. Allen, Elizabeth Anderson, Mary Assenmacher, Deana Barrett, Laurie Barringer, Linda Baty, Holidae Bauman, Leila Beach, Liesha Beachum, Jacquline S. Bean, Jane Betten, Melanie Black, Kari Blouin, Kelly Bottger, Nancy Bowman, JoAnn Britenfeld, Ruth C. Brower

Row 2: Melanie Mai Brown, Estera Carp, Christine Haveman, Kimberly Webster, Rebecca Amo, Tina K. Ciricola, Gerard A. Castaneda, Steven J. Bednarski, Michelle Kuo, Kathleen Szymanski, Alissa Enriquez, Kristin Snow, Jennifer Berk, Erica Reese, Angela Cassadime, Lynn Chacko

Row 3: Anne chambers, Mechele Chau, Marcy Christensen, Regi Colthorp, Kellie Conover, Jenny Cwiek, Michele DeMaagd, Kristin Diotte, Amanda Dressel, Kimberly Dunlap, Esther DuRussel, Katrina Ehr

Row 4: Holly M. Greenough, Eileen Gumayagay, Sheila Habib, Allison Hale, Kristi Hale, Amy Hollis, Rebecca Hostman, Marilynn Huizinga, Jennifer Ivinson, Christine Jodoin, Christine Kaetz, Kimberly Kenny-Sherlock, Andrea Latva, Kathleen Levin

Row 5: Shawna Mangan, Sofia Marquez, Paul Mazurek, Charla McMichael, Tina Marie Meeks, Richard W. Redman, Beverly Jones, Ada Sue Hinshaw, Susan Boehm, Nola Pender, Jeffrey Mendoza, Melissa Meulenberg, Cheryl Milekovich, Amanda Miller, Amy Miller

Row 6: Nicole Miller, Bonnie Mobley, Kara More, Cherylann Mortzfield, Leslie Nance, Ruby Nzoma, Megan Oleszek, Larah Faye Ostonal, Jean C. Palad, Danielle Pankowski, Nancy Penrose, Laurie Pierce, Heather Polsen, Julie Marie Postma, Amy Prielipp, Martha Quigley, Aimee Racette

Row 7: Marty Rauser, Nekia Robinson, Kimberly Rowe, Janice Rybski, Ricardo Salazar, Marie Sanderson, Kimberly Scholma, Bonnie Schweitzer, Veena Shewakramani, Julie Showers, Olga Simanovskaya, Emily Simon, Lakeeta Smith, Amy Stewart, Robert Strudgeon, Jaime Sulek

Row 8: Charity Sutherland, William Ten Haaf, Mark Thomas, Nichole Thomas, Donna Thompson, Tonya Tomski, Michele Uller, Celina Uranga, Sarah Volkhardt, Larna Welch, Melanie White, Michelle White, Britt Williams, Stephanie Windisch, Brian Wright, Christina Wyrybkowski, Lisa Ziegelmann

Baptism by fire : CIA analysis of the Korean War : a collection of previously released and recently declassified intelligence documents.

Mode of access: Internet.

Role of contact lenses in relieving ocular allergy

Purpose: To examine the potential barrier and lubricating effects of modern daily disposable contact lenses (DD) against airborne antigens. Methods: Ten patients with skin prick and ocular conjunctival provocation confirmed allergic sensitivity to grass pollen were recruited (average age 27.4±7.7 years). Each had their ocular symptoms (on a 0 none to 5 extreme scale) and appearance of bulbar and limbal conjunctival redness, palpebral conjunctival redness and roughness, and corneal and conjunctival fluorescein staining (CCLRU scale) graded before and five minutes after exposure to 400 grains grass pollen/m3 for 2 minutes in a purpose-designed exposure chamber to simulate the conditions of a ‘very high’ pollen-count day. This was repeated on three occasions separated by >72 hours wearing etafilcon A (sDD), nelfilcon A with enhanced lubricating agents (ELDD) and no contact lenses in random order out of the pollen season. Each sign and symptom was compared to baseline for each condition. The duration of the symptoms was also recorded. www.clinicaltrials.gov NCT01125540 Results: Only symptoms of burning and stinging were significantly reduced in severity by ELDD (Chi-Sq=7.6, p=0.02), but overall symptoms were significantly reduced in duration (F=3.60, p=0.05). Bulbar hyperaemia, corneal and conjunctival staining, and palpebral conjunctival roughness were significantly reduced by DD wear (p<0.01), with limbal and palpebral conjunctival redness further reduced in ELDD (p<0.05). Conclusion: Daily disposable contact lenses offer a barrier to airborne antigen which is enhanced by modern lenses with enhanced lubricating agents.

Catherine Tekakwitha et la peinture missionnaire : stratégies de conversion en Nouvelle-France au 17e siècle

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