Phylogenetic position of the acariform mites: sensitivity to homology assessment under total evidence

Background: Mites (Acari) have traditionally been treated as monophyletic, albeit composed of two major lineages: Acariformes and Parasitiformes. Yet recent studies based on morphology, molecular data, or combinations thereof, have increasingly drawn their monophyly into question. Furthermore, the usually basal (molecular) position of one or both mite lineages among the chelicerates is in conflict to their morphology, and to the widely accepted view that mites are close relatives of Ricinulei. Results: The phylogenetic position of the acariform mites is examined through employing SSU, partial LSU sequences, and morphology from 91 chelicerate extant terminals (forty Acariformes). In a static homology framework, molecular sequences were aligned using their secondary structure as guide, whereby regions of ambiguous alignment were discarded, and pre-aligned sequences analyzed under parsimony and different mixed models in a Bayesian inference. Parsimony and Bayesian analyses led to trees largely congruent concerning infraordinal, well-supported branches, but with low support for inter-ordinal relationships. An exception is Solifugae + Acariformes (P. P = 100%, J. = 0.91). In a dynamic homology framework, two analyses were run: a standard POY analysis and an analysis constrained by secondary structure. Both analyses led to largely congruent trees; supporting a (Palpigradi (Solifugae Acariformes)) clade and Ricinulei as sister group of Tetrapulmonata with the topology (Ricinulei (Amblypygi (Uropygi Araneae))). Combined analysis with two different morphological data matrices were run in order to evaluate the impact of constraining the analysis on the recovered topology when employing secondary structure as a guide for homology establishment. The constrained combined analysis yielded two topologies similar to the exclusively molecular analysis for both morphological matrices, except for the recovery of Pedipalpi instead of the (Uropygi Araneae) clade. The standard (direct optimization) POY analysis, however, led to the recovery of trees differing in the absence of the otherwise well-supported group Solifugae + Acariformes. Conclusions: Previous studies combining ribosomal sequences and morphology often recovered topologies similar to purely morphological analyses of Chelicerata. The apparent stability of certain clades not recovered here, like Haplocnemata and Acari, is regarded as a byproduct of the way the molecular homology was previously established using the instrumentalist approach implemented in POY. Constraining the analysis by a priori homology assessment is defended here as a way of maintaining the severity of the test when adding new data to the analysis. Although the strength of the method advocated here is keeping phylogenetic information from regions usually discarded in an exclusively static homology framework; it still has the inconvenience of being uninformative on the effect of alignment ambiguity on resampling methods of clade support estimation. Finally, putative morphological apomorphies of Solifugae + Acariformes are the reduction of the proximal cheliceral podomere, medial abutting of the leg coxae, loss of sperm nuclear membrane, and presence of differentiated germinative and secretory regions in the testis delivering their products into a common lumen.

Complete Genome Viral Phylogenies Suggests the Concerted Evolution of Regulatory Cores and Accessory Satellites

We consider the concerted evolution of viral genomes in four families of DNA viruses. Given the high rate of horizontal gene transfer among viruses and their hosts, it is an open question as to how representative particular genes are of the evolutionary history of the complete genome. To address the concerted evolution of viral genes, we compared genomic evolution across four distinct, extant viral families. For all four viral families we constructed DNA-dependent DNA polymerase-based (DdDp) phylogenies and in addition, whole genome sequence, as quantitative descriptions of inter-genome relationships. We found that the history of the polymerase gene was highly predictive of the history of the genome as a whole, which we explain in terms of repeated, co-divergence events of the core DdDp gene accompanied by a number of satellite, accessory genetic loci. We also found that the rate of gene gain in baculovirus and poxviruses proceeds significantly more quickly than the rate of gene loss and that there is convergent acquisition of satellite functions promoting contextual adaptation when distinct viral families infect related hosts. The congruence of the genome and polymerase trees suggests that a large set of viral genes, including polymerase, derive from a phylogenetically conserved core of genes of host origin, secondarily reinforced by gene acquisition from common hosts or co-infecting viruses within the host. A single viral genome can be thought of as a mutualistic network, with the core genes acting as an effective host and the satellite genes as effective symbionts. Larger virus genomes show a greater departure from linkage equilibrium between core and satellites functions.

Bringing people back in: Grassroots approach to peace in Bosnia-Nerzegovina

Peacemaking in Bosnia-Herzegovina is a controversial subject that engaged the political energies of the international community for several years without resolution. While international efforts at peacemaking warrant a critique in their own right, the assumptions that lie behind popular perceptions of peacemaking must also be examined. This article explores the proposition that the promotion of multi-ethnic contact between Bosnian people is at least as important as elections or the reconstruction of political institutions. Indeed, the restoration and development of inter-ethnic relationships, especially at grassroots level, is essential for the establishment of sustainable peace. This article thus focuses on the roles of NGOs (Non-governmental organisations) active at the grassroots level in Bosnia, such as the Centre for Drama Education in Mostar, Project Firefly in Brcko, and the CARE Welcome Project in Sarajevo, which represent a grassroots form of peacemaking that incorporates local knowledge and understandings of the conflict within peace projects.

Auditoria e gestão : um estudo de caso dos trabalhos auditoriais realizados no IFES

Por intermédio deste trabalho, foi possível detectar o acúmulo de recomendações, o que possivelmente possa indicar a presença de falhas na gestão. Dessa forma o objetivo deste trabalho é investigar as causas e propor soluções que contribuam para o atendimento das recomendações pendentes e para a melhoria de tomada de decisões administrativas. Tomou-se como apoio o Relatório de Auditoria Anual de Contas nº 201305863 e forma apresentadas ferramentas que auxiliem a gestão no processo gerencial de tomadas de decisões. O universo pesquisado compõe-se dos gestores do IFES, que vivem a rotina burocrática dessa instituição de ensino, o que possibilita obter uma percepção do inter-relacionamento profissional junto à AUDIN (Auditoria Interna). A coleta de dados foi feita por meio de questionário, tendo foco na percepção dos gestores de como os trabalhos de auditoria são realizados no IFES, e, paralelamente, buscou informações capazes de municiar a AUDIN com ferramentas capazes de melhor auxiliar no assessoramento administrativo. A pesquisa sugere que a Auditoria Interna e os gestores incorporem diversos conhecimentos para se obter um melhor desempenho frente às demandas do controle interno e externo. Foi observado um acúmulo significativo de recomendações, o que indica a presença de possíveis falhas na gestão do referido órgão. Assim sendo, a pesquisa apresentou resultados que posicionam a Auditoria Interna à margem do processo gerencial do IFES.

Combining the rational and relational perspectives of electronic trading

Many organisations make extensive use of electronic linkages to facilitate their trading exchanges with partners such as suppliers, distributors and customers. This research explores how the use of inter-organisational systems (IOS) both affects, and is affected by, the relationships between trading partners. In doing this, it brings together two existing but distinct perspectives and literatures; the rational view informed by IOS research, and the behavioural or relationship perspective embodied in inter-organisational relationships (IOR) literature. The research was undertaken in the European paper industry by means of six dyadic case studies. The dyads studied covered both traditional electronic data interchange systems and newer e-marketplace environments. A framework was derived from existing literature that integrates the two perspectives of interest. The framework was used to analyse the case studies undertaken and enabled the inter-relationship between IOS use and IOR to be explained.

A autonomia na resolução de conflitos interpessoais em jardim de infância

Relatório da Prática Profissional Supervisionada Mestrado em Educação Pré-Escolar

Ambivalent Islam: the identity construction of Muslims under Portuguese colonial rule

This article wishes to contribute to the study of the historical processes that have been spotting Muslim populations as favourite targets for political analysis and governance. Focusing on the Portuguese archives, civil as well as military, the article tries to uncover the most conspicuous identity representations (mainly negative or ambivalent) that members of Portuguese colonial apparatus built around Muslim communities living in African colonies, particularly in Guinea-Bissau and Mozambique. The paper shows how these culturally and politically constructed images were related to the more general strategies by which Portuguese imagined their own national identity, both as ‘European’ and as ‘coloniser’ or ‘imperial people’. The basic assumption of this article is that policies enforced in a context of inter-ethnic and religious competition are better understood when linked to the identity strategies inherent to them. These are conceived as strategic constructions aimed at the preservation, the protection and the imaginary expansion of the subject, who looks for groups to be included in and out-groups to reject, exclude, aggress or eliminate. We think that most of the inter-ethnic relationships and conflicts, as well as the very experience of ethnicity, are born from this identity matrix.

Ambivalent Islam: the identity construction of Muslims under Portuguese colonial rule

This article wishes to contribute to the study of the historical processes that have been spotting Muslim populations as favourite targets for political analysis and governance. Focusing on the Portuguese archives, civil as well as military, the article tries to uncover the most conspicuous identity representations (mainly negative or ambivalent) that members of Portuguese colonial apparatus built around Muslim communities living in African colonies, particularly in Guinea- Bissau and Mozambique. The paper shows how these culturally and politically constructed images were related to the more general strategies by which Portuguese imagined their own national identity, both as ‘European’ and as ‘coloniser’ or ‘imperial people’. The basic assumption of this article is that policies enforced in a context of interethnic and religious competition are better understood when linked to the identity strategies inherent to them. These are conceived as strategic constructions aimed at the preservation, protection and imaginary expansion of the subject, who looks for groups to be included in and out-groups to reject, exclude, aggress or eliminate. The author argues that most of the inter-ethnic relationships and conflicts, as well as the very experience of ethnicity, are born from this identity matrix.

Mechanisms of agglomeration: Evidence from the location of new manufacturing firms in Spain

El objetivo de esta investigación es aportar evidencia sobre las fuentes de las economías de aglomeración para el caso español. De todas las maneras posibles que se han tomado en la literatura para medir las economías de aglomeración, nosotros lo analizamos a partir de las decisiones de localización de las empresas manufactureras. La literatura reciente ha puesto de relieve que el análisis basado en la disyuntiva localización / urbanización (relaciones dentro de un mismo sector) no es suficiente para entender las economías de aglomeración. Sin embargo, las relaciones entre los diferentes sectores sí resultan significativas al examinar por qué las empresas que pertenecen a diferentes sectores se localizan unas al lado de las otras. Con esto en mente, intentamos explicar que relaciones entre diferentes sectores pueden explicar coaglomeración. Para ello, nos centramos en aquellas relaciones entre sectores definidos a partir de los mecanismos de aglomeración de Marshall, es decir, labor market, input sharing y knowledge spillovers. Trabajamos con el labor market pooling en la medida en que los dos sectores utilizan los mismos trabajadores (clasificación de ocupaciones). Con el segundo mecanismo de Marshall, input sharing, introducimos cómo dos sectores tienen una relación de comprador / vendedor. Por último, nos referimos a dos sectores que utilizan las mismas tecnologías en cuanto a los knowledge spillovers. Con el fin de capturar todos los efectos de los mecanismos de aglomeracion en España, en esta investigación trabajamos con dos ámbitos geográficos, los municipios y los mercados de trabajo locales. La literatura existente nunca se ha puesto de acuerdo en cual es el ámbito geográfico en el que mejor trabajan los mecanismos Marshall, por lo que hemos cubierto todas las unidades geográficas potenciales.

Bovine tuberculosis in Doñana Biosphere Reserve: the role of wild ungulates as disease reservoirs in the last Iberian lynx strongholds.

Doñana National Park (DNP) in southern Spain is a UNESCO Biosphere Reserve where commercial hunting and wildlife artificial feeding do not take place and traditional cattle husbandry still exists. Herein, we hypothesized that Mycobacterium bovis infection prevalence in wild ungulates will depend on host ecology and that variation in prevalence will reflect variation in the interaction between hosts and environmental risk factors. Cattle bTB reactor rates increased in DNP despite compulsory testing and culling of infected animals. In this study, 124 European wild boar, 95 red deer, and 97 fallow deer were sampled from April 2006 to April 2007 and analyzed for M. bovis infection. Modelling and GIS were used to identify risk factors and intra and inter-species relationships. Infection with M. bovis was confirmed in 65 (52.4%) wild boar, 26 (27.4%) red deer and 18 (18.5%) fallow deer. In the absence of cattle, wild boar M. bovis prevalence reached 92.3% in the northern third of DNP. Wild boar showed more than twice prevalence than that in deer (p<0.001). Modelling revealed that M. bovis prevalence decreased from North to South in wild boar (p<0.001) and red deer (p<0.01), whereas no spatial pattern was evidenced for fallow deer. Infection risk in wild boar was dependent on wild boar M. bovis prevalence in the buffer area containing interacting individuals (p<0.01). The prevalence recorded in this study is among the highest reported in wildlife. Remarkably, this high prevalence occurs in the absence of wildlife artificial feeding, suggesting that a feeding ban alone would have a limited effect on wildlife M. bovis prevalence. In DNP, M. bovis transmission may occur predominantly at the intra-species level due to ecological, behavioural and epidemiological factors. The results of this study allow inferring conclusions on epidemiological bTB risk factors in Mediterranean habitats that are not managed for hunting purposes. Our results support the need to consider wildlife species for the control of bTB in cattle and strongly suggest that bTB may affect animal welfare and conservation.

On the industry experience premium and labor mobility

[cat] Hi ha evidència que l'experiència es remunera diferentment segons la indústria. Proposem un model teòric que explica aquestes diferències. Suposem que la mobilitat de treballadors aporta coneixement extern a l'empresa i això augmenta la seva productivitat. Els resultats mostren que l'experiència és millor remunerada en les indústries amb costos de mobilitat baixos, amb molt aprenentatge (learning-by-doing) i alt nivell tecnològic. A més, trobem una relació en forma de U entre la remuneració de l'experiència i el nivell d'absorció de coneixement extern, la substitutibilitat entre diferents tipus de treballadors i la varietat de coneixement dins la indústria. Els resultats són consistents amb l'evidència que les indústries intensives en I and D remuneren millor l'experiència.

On the industry experience premium and labor mobility

[cat] Hi ha evidència que l'experiència es remunera diferentment segons la indústria. Proposem un model teòric que explica aquestes diferències. Suposem que la mobilitat de treballadors aporta coneixement extern a l'empresa i això augmenta la seva productivitat. Els resultats mostren que l'experiència és millor remunerada en les indústries amb costos de mobilitat baixos, amb molt aprenentatge (learning-by-doing) i alt nivell tecnològic. A més, trobem una relació en forma de U entre la remuneració de l'experiència i el nivell d'absorció de coneixement extern, la substitutibilitat entre diferents tipus de treballadors i la varietat de coneixement dins la indústria. Els resultats són consistents amb l'evidència que les indústries intensives en I and D remuneren millor l'experiència.

Yritysten välisen testaus- ja laboratoriotoiminnan kehittäminen Päijät-Hämeessä

Yritykset tekevät nykyään yhä enemmän yhteistyötä keskenään eli verkostoituvat. Verkostoitumisella haetaan kykyä vastata nopeasti muuttuvan toimintaympäristön haasteisiin. Tässä diplomityössä tutkitaan testaus- ja laboratoriopalveluiden saatavuuden kehittämistä verkostoitumisen keinoin.Alueellisia testaus- ja laboratoriopalveluiden saatavuutta parannetaan valjastamalla käyttöön yritysten yksityisessä käytössä olevat tuotekehityslaboratoriot ja niiden vapaa kapasiteetti. Verkoston ytimen muodostaa teknologiapalvelu, joka hallitsee tietoa yritysten resursseista ja jakaa tätä tietoa. Työssä kartoitettiin alueella olevista yrityksistä heidän tarpeitaan tuotekehityksen aikaiselle testaukselle, tähän sopivia olemassa olevia laitteita ja tiloja ja yritysten mielipiteitä toiminnan organisoimisesta. Lisäksi tutkittiin tuotekehityksen aikana tarvittavaa direktiivitietouden tarvetta yrityksissä. Yritykset suhtautuivat erittäin myönteisesti yhteistyön tekemiseen testaus- ja laboratoriotoiminnassa. Yrityksistä löytyi erilaisia testauslaitteita ja –tiloja, ja näitä on muidenkin yritysten mahdollista käyttää tietyin ehdoin, kunhan se ei häiritse yrityksen omaa tuotekehitystyötä ja vapaata kapasiteettia löytyy.

Verkostoitumisen hyödyt ja haitat teollisuusrakennusten kokonaistoimituksissa

Lemcon Oy on Lemminkäinen konserniin kuuluva kansainvälinen projektirakentaja. Tässä työssä kuvataan Lemcon Oy:n ja mahdollisten partnereiden pidempiaikaisen yhteistyön hyötyjä, haittoja ja riskejä Lemcon Oy:n näkökulmasta. Tarkastelu rajataan sellaisiin yhteistyökumppaneihin, jotka ovat itsekin kilpailutilanteessa asiakkaisiin nähden ja jotka voisivat käyttää Lemcon Oy:tä alihankkijana tehdas- tai voimalaitosrakennusten rakennusteknisten töiden kokonaistoimituksissa.. Diplomityö on tehty pääosin kirjallisuustutkimuksena. Lisäksi työssä kuvataan kahden mahdollisen yhteistyökumppanin mielipiteitä ja käsityksiä mahdollisesta yhteistyöstä. Tätä varten on haastateltu yhteensä 6 henkilöä kahdesta yrityksestä. Kirjallisuustutkimuksen mukaan pidempiaikainen yhteistyö olisi molemmille osapuolille kannattavaa. Mahdolliset partnerit osoittivat varovaista kiinnostusta pidempiaikaista yhteistyötä kohtaan ja olivat valmiit jatkoneuvotteluihin.

Yritysten väliset verkostot tietojohtamisen näkökulmasta – Case Imatran Seudun Koivukeskus

Alueellinen yrityskeskittymä sisältää monenlaisia yhteistyöverkostoja siinä toimivien yritysten välillä. Olemassa olevien verkosto- ja tietojohtamisteorioiden pohjalta yhteistyöverkostot jaettiin niiden perustehtävän mukaan kolmeen eri päätyyppiin, jotka ovat tuottaja-, kehittäjä- ja innovaatioverkosto. Tutkimusongelmana oli määrittää, voidaanko verkostoja eritellä niiden perustehtävän mukaan ja näkyykö perustehtävän erilaisuus niiden organisoitumis- ja kommunikointitavassa. Tutkimuskohteena oli kasvava puualan keskittymä, Imatran Seudun Koivukeskus. Tutkimusmenetelmänä käytettiin tapaustutkimusta ja aineistonkeruumenetelmänä teemahaastattelua. Aineistoa käsiteltiin käyttämällä menetelmänä profilointia ja teemoittelua. Tutkimuksen perusteella voitiin alueelta erottaa selvästi kolme verkostojen päätyyppiä, jotka toimivat alueella tietoa luovana, välittävänä ja soveltavana tasona. Päätyyppien välillä havaittiin eroja, kun kriteereinä käytettiin verkostojen suhteita, osaamista, tiedonkulkua ja johtajuutta. Koivukeskukselle erityisen tärkeänä verkostotyyppinä pidettiin kehittäjäverkostoa, sillä se toimii alueen sosiaalisen pääoman kasvualustana ja täten auttaa muiden verkostotyyppien kehittymistä. Mikäli kaikki päätyypit saadaan toimi-maan tehokkaasti, alueen innovaatioverkostossa syntyvät uudet prosessi- ja tuoteinnovaatiot ovat välittömästi siirrettävissä tehostamaan tuotantoa. Alueellinen yrityskeskittymä sisältää kaikkia edellä mainittuja verkostotyyppejä, ja yksittäinen toimija voi kuulua samanaikaisesti kaikkiin verkostotyyppeihin. Johtopäätöksenä esitettiin, että alueellisen yrityskeskittymän kilpailuetu syntyy alueen sisälle muodostuvien tuottaja-, kehittäjä- ja innovaatioverkoston kautta. Alueellisen yrityskeskittymän verkostopohjaisen kehittämisen avuksi kehitettiin yksinkertainen työkalu.