Étude du niveau de B Lymphocyte Stimulator (BLyS) et de son impact sur les lymphocytes B en relation avec l’infection et la résistance au virus d’immunodéficience humaine (VIH) chez des travailleuses du sexe au Bénin

L’infection au VIH s’accompagne souvent de dérégulations du compartiment des lymphocytes B qui nuisent à la génération de réponses efficaces. En effet, détectées tôt après l’infection, ces dérégulations perdurent, ne sont pas totalement restaurées par la thérapie, et mènent souvent à des manifestations auto-immunes et lymphomes. Une étude longitudinale de notre groupe, effectuée avec des cellules mononucléées du sang circulant provenant de patients VIH+ avec différents types de progression clinique, a démontré qu’un niveau élevé de BLyS chez des individus VIH+ progresseurs était associé à une dérégulation des fréquences de populations de cellules B avec augmentation de cellules innées de la zone marginale (MZ) présentant des caractéristiques d’immaturité et d’activation. Au contraire, chez des individus VIH+ non-progresseurs avirémiques ou contrôleurs d’élite, les niveaux de BLyS étaient dans la normale et ce sont les fréquences de cellules B MZ plus matures qui étaient diminuées. La résistance au VIH pourrait aussi impliquer le contrôle de BLyS et son impact sur les cellules B. De ce fait, nous avons préalablement recruté une cohorte de travailleuses du sexe (TS) à Cotonou (Bénin) dans laquelle nous avons identifié des femmes qui demeurent séronégatives malgré une exposition soutenue au virus. Nous avons mesuré les niveaux de BLyS dans le sang et dans les lavages cervico-vaginaux (CVL) de TS VIH- et les avons comparés à ceux mesurés chez des TS VIH+ et un groupe contrôle de non-TS VIH- . Nous avons trouvé que les niveaux de BLyS dans le sang et le CVL des TS VIH- étaient inférieurs à ceux des TS VIH+ et des non-TS VIH-. Le niveau d’expression de BLyS à la surface des lymphocytes T, monocytes et cellules dendritiques de TS VIH- était augmenté, mais à un niveau moindre que les TS VIH+. Chez les TS VIH+, les hauts niveaux de BLyS étaient concomitants avec une dérégulation du compartiment B caractérisée par une hyperglobulinémie, une augmentation de la fréquence de populations avec un profil immature/inné et une plus grande proportion de plasmablastes IgG vs IgA. Au contraire, les niveaux inférieurs de BLyS dans le sang des TS VIH- coïncident avec un compartiment B préservé, révélant que les lymphocytes B MZ peuvent être impliqués dans l’immunité naturelle au VIH. Ces résultats démontrent l’importance du contrôle des niveaux de BLyS et du maintien de l’intégrité du compartiment B dans la résistance au VIH.

Application of scaled particle theory to alkyl-aryl surfactants: a two zone model of micellar core

Scaled Particle Theory (SPT) has been applied to predict the total free energies of micellization of ionic as well as nonionic micellar systems containing an aryl ring. A modification of the previously developed model has been made, proposing a two-zone model of micellar core which corroborates with the structural information available for such systems. The results are in good agreement with experimental data and also confirm the dictating role of cavity forming free energies for such systems

Multi-zone TAP-reactors theory and application - IV. Ideal and non-ideal boundary conditions

We study the influence of non-ideal boundary and initial conditions (BIC) of a temporal analysis of products (TAP) reactor model on the data (observed exit flux) analysis. The general theory of multi-response state-defining experiments for a multi-zone TAP reactor is extended and applied to model several alternative boundary and initial conditions proposed in the literature. The method used is based on the Laplace transform and the transfer matrix formalism for multi-response experiments. Two non-idealities are studied: (1) the inlet pulse not being narrow enough (gas pulse not entering the reactor in Dirac delta function shape) and (2) the outlet non-ideality due to imperfect vacuum. The effect of these non-idealities is analyzed to the first and second order of approximation. The corresponding corrections were obtained and discussed in detail. It was found that they are negligible. Therefore, the model with ideal boundary conditions is proven to be completely adequate to the description and interpretation of transport-reaction data obtained with TAP-2 reactors.

Granular flow in the marginal ice zone

The region of sea ice near the edge of the sea ice pack is known as the marginal ice zone (MIZ), and its dynamics are complicated by ocean wave interaction with the ice cover, strong gradients in the atmosphere and ocean and variations in sea ice rheology. This paper focuses on the role of sea ice rheology in determining the dynamics of the MIZ. Here, sea ice is treated as a granular material with a composite rheology describing collisional ice floe interaction and plastic interaction. The collisional component of sea ice rheology depends upon the granular temperature, a measure of the kinetic energy of flow fluctuations. A simplified model of the MIZ is introduced consisting of the along and across momentum balance of the sea ice and the balance equation of fluctuation kinetic energy. The steady solution of these equations is found to leading order using elementary methods. This reveals a concentrated region of rapid ice flow parallel to the ice edge, which is in accordance with field observations, and previously called the ice jet. Previous explanations of the ice jet relied upon the existence of ocean currents beneath the ice cover. We show that an ice jet results as a natural consequence of the granular nature of sea ice.

Does water level affect benthic macro-invertebrates of a marginal lake in a tropical river-reservoir transition zone?

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Observations and modeling of the Marginal Ice Zone

Global climate change in recent decades has strongly influenced the Arctic generating pronounced warming accompanied by significant reduction of sea ice in seasonally ice-covered seas and a dramatic increase of open water regions exposed to wind [Stephenson et al., 2011]. By strongly scattering the wave energy, thick multiyear ice prevents swell from penetrating deeply into the Arctic pack ice. However, with the recent changes affecting Arctic sea ice, waves gain more energy from the extended fetch and can therefore penetrate further into the pack ice. Arctic sea ice also appears weaker during melt season, extending the transition zone between thick multi-year ice and the open ocean. This region is called the Marginal Ice Zone (MIZ). In the Arctic, the MIZ is mainly encountered in the marginal seas, such as the Nordic Seas, the Barents Sea, the Beaufort Sea and the Labrador Sea. Formed by numerous blocks of sea ice of various diameters (floes) the MIZ, under certain conditions, allows maritime transportation stimulating dreams of industrial and touristic exploitation of these regions and possibly allowing, in the next future, a maritime connection between the Atlantic and the Pacific. With the increasing human presence in the Arctic, waves pose security and safety issues. As marginal seas are targeted for oil and gas exploitation, understanding and predicting ocean waves and their effects on sea ice become crucial for structure design and for real time safety of operations. The juxtaposition of waves and sea ice represents a risk for personnel and equipment deployed on ice, and may complicate critical operations such as platform evacuations. The risk is difficult to evaluate because there are no long-term observations of waves in ice, swell events are difficult to predict from local conditions, ice breakup can occur on very short time-scales and wave-ice interactions are beyond the scope of current forecasting models [Liu and Mollo-Christensen, 1988,Marko, 2003]. In this thesis, a newly developed Waves in Ice Model (WIM) [Williams et al., 2013a,Williams et al., 2013b] and its related Ocean and Sea Ice model (OSIM) will be used to study the MIZ and the improvements of wave modeling in ice infested waters. The following work has been conducted in collaboration with the Nansen Environmental and Remote Sensing Center and within the SWARP project which aims to extend operational services supporting human activity in the Arctic by including forecast of waves in ice-covered seas, forecast of sea-ice in the presence of waves and remote sensing of both waves and sea ice conditions. The WIM will be included in the downstream forecasting services provided by Copernicus marine environment monitoring service.

Cancellation zone in supersonic lifting wing theory

BASING their work on a linear theory, Evvard1 and Krasilshchikova2'3 independently developed an expression that yields the perturbation generated by a thiri lifting wing of arbitrary planform flying at supersonic speed on a point placed on the wing plane inside its planform,1 or both on and above the wing plane.2 This point must be influenced by two leading edges, one supersonic and the other partially subsonic. Although these authors followed different approaches, their methods concur in showing the existence of a perfectly defined cancellation zone. In this Note, the Evvard approach is generalized to the case solved by Krasilshchikova. Circumventing the latter's lengthy and somewhat complex approach, Evvard's simple method seems to be useful at least for educational purposes.

(Table 1) Physical oceanography during Lance cruise LA99/2 in the marginal ice zone of the Barents Sea

Zooplankton was studied on eight stations in the marginal ice zone (MIZ) of the Barents Sea, in May 1999, along two transects across the ice edge. On each station, physical background measurements and zooplankton samples were taken every 6 h over a 24 h period at five discrete depth intervals. Cluster analysis revealed separation of open water stations from all ice stations as well as high similarity level among replicates belonging to particular station. Based on five replicates per station, analysis of variance (ANOVA) confirmed significant differences (P < 0.05) in abundances of the main mesozooplankton taxa among stations. Relations between the zooplankton community and environmental parameters were established using redundancy analysis (CANOCO). In total, 55% of mesozooplankton variability within studied area was explained by eight variables with significant conditional effects: depth stratum, fluorescence, temperature, salinity, bottom depth, latitude, bloom situation, and ice concentration. GLM models supported supposition about clear and negative relationship between concentration of Oithona similis, and overall mesozooplankton diversity The analyses showed a dynamic relationship between mesozooplankton distribution and hydrological conditions on short-term scale. Furthermore, our study demonstrated that variability in the physical environment of dynamic MIZ of the Barents Sea has measurable effect on the Arctic pelagic ecosystem.

Airborne remote sensing of ocean wave directional wavenumber spectra in the marginal ice zone

Interactions between surface waves and sea ice are thought to be an important, but poorly understood, physical process in the atmosphere-ice-ocean system. In this work, airborne scanning lidar was used to observe ocean waves propagating into the marginal ice zone (MIZ). These represent the first direct spatial measurements of the surface wave field in the polar MIZ. Data were compared against two attenuation models, one based on viscous dissipation and one based on scattering. Both models were capable of reproducing the measured wave energy. The observed wavenumber dependence of attenuation was found to be consistent with viscous processes, while the spectral spreading of higher wavenumbers suggested a scattering mechanism. Both models reproduced a change in peak direction due to preferential directional filtering. Floe sizes were recorded using co-located visible imagery, and their distribution was found to be consistent with ice breakup by the wave field.