83 resultados para Ginga-NCL


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Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Ao conceber esta obra, Evaldo Aparecido de Abreu levou em conta o decreto que instituiu o Sistema Brasileiro de TV Digital (SBTVD), que lista entre os objetivos da tecnologia propiciar a criação de rede universal de educação a distância. Ele estudou as atividades que deve desempenhar o profissional responsável por adequar material didático impresso, como livros e apostilas, em material audiovisual, mais especificamente para explorar a interatividade da TV. De todo o processo, o livro analisa a etapa da transformação do conteúdo pedagógico em produto audiovisual, chamado de design instrucional. E destaca o papel que o coordenador geral dessa atividade deve desempenhar. A obra levanta questões como as características tecnológicas da TV digital e do middleware Ginga, o software promotor da interatividade, e discute métodos de adaptação do material didático para a TV digital. Inclui ainda reflexões sobre a importância da interatividade no desenvolvimento de projetos educacionais e sobre como estes podem contribuir para a melhoria da educação brasileira.

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The presence of oropharyngeal dysphagia in the pediatric population with genetic diseases it is still poorly studied. The aim of this study was to analyze the oral total transit time and pharyngeal transit time, in an individual with neuronal ceroid lipofucinosis (NCL) with severe oropharyngeal dysphagia. Individual with NCL, 3 years old, 2 years with gastrostomy and no oral feeding, weighting loss, but without pulmonary complications. Oropharyngeal swallowing was studied by videofluoroscopy and it was realized a quantitative analysis using software. Changes were observed throughout the whole biomechanics of swallowing. The quantitative analysis of total oral transit time was found 45.37 seconds (default normality in children is 4 seconds) and for pharyngeal transit time was 4.53 seconds. It was found that beside the changes in the biomechanics of oropharyngeal swallowing in the case studied, an increase in total oral transit time and pharyngeal transit time was also observed, which can significantly compromise the nutritional status and pulmonary these individuals.

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Pós-graduação em Comunicação - FAAC

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This work had as objective the development of a prototype of educational content, as an experimental process, making use of resources from Interactive Digital TV. The characteristics of interactive digital terrestrial television are presented, in the form of a media mode capable of performing as a complementary source to education. The methodology used is composed of four phases: deduction, analysis, induction and synthesis. Its conception takes as issue the expressed need stated by UNESCO to use ICT (Information and Communications Technology) towards education, as well as the possibility of applying digital television in an educational process. The content is structured in the form of a quiz, a type of question-answer game as an informal way of education for high school students. The prototype developed in this work is presented as a structural script and map, taking into account the necessary activities and routines to put it into operation in the tested environments, eclipse, virtual set-top-box and Developer Box. The practice of prototyping was carried out in NCLua, based on middleware Ginga. As a result, we concluded that Ginga offers enough support to carry out the task, providing characteristics of interaction to publish the proposed content, at the moment of the actual practice.

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Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

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Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

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Digital Television offers a resolution of highdefinition images and the possibility of interaction with the user. There is an estimate that by 2018 all municipalities in Brazil have digital signal availability and thus there will be a wide spread of Ginga. Ginga is a technology developed for digital television, which runs between the operating system and yours applications. However, the extensive use of Smart TVs would invalidate this technology? This article to cast a look on the topic, arousing reflection that effect and seeking bring issues that link the advantages and disadvantages of the two technologies, considering the current situation of Digital TV in Brazil.

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In this paper we discuss the problem of how to discriminate moments of interest on videos or live broadcast shows. The primary contribution is a system which allows users to personalize their programs with previously created media stickers-pieces of content that may be temporarily attached to the original video. We present the system's architecture and implementation, which offer users operators to transparently annotate videos while watching them. We offered a soccer fan the opportunity to add stickers to the video while watching a live match: the user reported both enjoying and being comfortable using the stickers during the match-relevant results even though the experience was not fully representative.

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KurzfassungrnrnZiel der vorliegenden Arbeit war es eine gezielte, hochspezifische Inhibierung der Proteinbiosynthese zu erreichen. Dies kann durch eine Blockierung des mRNA-Strangs durch komplementäre DNA/RNA-Stränge (ähnlich zur Antisense-Methode) oder durch die Hydrolyse des mRNA-Strangs mit Hilfe spezieller Enzyme (RNasen) realisiert werden. Da jedoch beide Methoden nicht zu zufriedenstellenden Ergebnissen führen, wäre deshalb eine Kombination aus beiden Methoden ideal, welche in einer spezifischen, gezielten und permanenten Ausschaltung der Proteinbiosynthese resultieren würde. Um dieses Ziel zu verwirklichen, ist es nötig, ein Molekül zu synthetisieren, welches in der Lage ist selektiv an einer spezifischen Position an den RNA-Strang zu hybridisieren und anschließend den RNA-Strang an dieser zu hydrolysieren. Der große Vorteil dieses Konzepts liegt darin, dass die DNA-Sequenz für die Hybridisierung an die entsprechende RNA maßgeschneidert hergestellt werden kann und somit jede RNA gezielt angesteuert werden kann, was letztendlich zu einer spezifischen Inhibierung der korrespondierenden Proteinbiosynthese führen soll.rnDurch die Verwendung und Optimierung der Nativen Chemischen Ligation (NCL) als Konjugationsmethode konnten zwei Biomakromoleküle in Form einer 46-basenlangen DNA (komplementär zum RNA-Strang) und einer 31-aminosäurelangen RNase kovalent verknüpft werden. Durch unterschiedliche chemische und molekularbiologische Analysemethoden, wie PAGE, GPC, CE, MALDI-ToF-MS etc., war es zudem möglich, die erfolgreiche Synthese dieses biologischen Hybridpolymers als monodisperses, reines Produkt zu bestätigen. rnDie Synthese des ca. 800-basenlangen RNA-Strangs, der als Modell-Matrize für die selektive und spezifische Degradierung durch das DNA-RNase-Konjugat dienen sollte, konnte unter Zuhilfenahme gentechnologischer Standard-Methoden erfolgreich bewerkstelligt werden. Weiterhin konnte durch die Verwendung der radioaktiven cDNA-Synthese gezeigt werden, dass das DNA-RNase-Konjugat an die gewünschte Stelle des RNA-Strangs hybridisiert. Die Identifizierung einer anschließenden spezifischen Hydrolyse des RNA-Strangs durch die an den DNA-Strang angeknüpfte RNase war aufgrund der geringen katalytischen Aktivität des Enzyms bisher allerdings nicht möglich.rn

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The present article gives a survey over the current scientific knowledge of the canine neuronal ceroid-lipofuscinosis (NCL). NCL is a heterogenous group of lysosomal storage diseases in humans and animals. In consequence of a gene mutation, there is an accumulation of ceroid-lipofuscin in neurons, cells of the retina and the skin and other cells. The stored ceroid-lipofuscin in neurons leads to an impaired cell function and subsequently to cell death. Recently, the underlying genetic defect was discovered in several dog breeds. Genetic testing permits an ante mortem diagnosis of the disease, which up to now was only possible with a positive biopsy result. Another advantage is the identification of carrier animals to eliminate the deleterious alleles.

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To provide insights into the long-term evolution of aquatic ecosystems without human interference, we here evaluate a decadal- to centennial-scale-resolution diatom record spanning about 12 ka of the Holsteinian interglacial (Marine Isotope Stage 11c). Using a partially varved sediment core from the Dethlingen palaeolake (northern Germany), which has previously been studied for palynological and microfacies signals, we document the co-evolution of the aquatic and surrounding terrestrial environment. The diatom record is dominated by the genera Stephanodiscus, Aulacoseira, Ulnaria and Fragilaria. Based on the diatom assemblages and physical sediment properties, the evolution of the Dethlingen palaeolake can be subdivided into three major phases. During the oldest phase (lasting ~1900 varve years), the lake was ~10-15 m deep and characterized by anoxic bottom-water conditions and a high nutrient content. The following ~5600 years exhibited water depths >20 m, maximum diatom and Pediastrum productivity, and a peak in allochtonous nutrient input. During this phase, water-column mixing became more vigorous, resulting in a breakdown of anoxia. The youngest lake phase (~4000-5000 years) was characterized by decreasing water depth, turbulent water conditions and decreased nutrient loading. Based on our palaeolimnological data, we conclude that the evolution of the Dethlingen palaeolake during the Holsteinian interglacial responded closely to (i) changes within the catchment area (as documented by vegetation and sedimentation) related to the transition from closed forests growing on nutrient-rich soils (mesocratic forest phase) to open forests developing on poor soils (oligocratic forest phase), and (ii) short-term climate variability as reflected in centennial-scale climate perturbations.

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Com o avanço da tecnologia e chegada da TV digital no Brasil, estudos apontam que a televisão aberta começa um novo estágio desde seu surgimento. Esta mídia disponibiliza ferramentas que permitem aos emissores de conteúdo uma dimensão interativa ainda não experimentada, sobretudo nos processos de comunicação massivos indicando que é possível novos caminhos de investimento na produção de conteúdo por parte das emissoras de TV. Com esta realidade de fundo, experiências começam a ser testadas no âmbito da televisão, como o StickerCenter, um software - desenvolvido pela TOTVS - que reúne elementos da internet na TV possibilitando ao receptor maior interação com o conteúdo televisivo, outra experiência é a realizada pela Rede Integração, afiliada à Rede Globo em Minas Gerais, com o software desenvolvido pela HXD Interactive Television, que testa um modelo brasileiro de interatividade no telejornal. Este trabalho mostra como estas duas empresas estão desenvolvendo aplicativos interativos para a TV utilizando o Middleware Ginga e os motivos pelos quais sua popularização não aconteceu. O StickerCenter não se popularizou e indica que o motivo seja erro de estratégia da empresa em desenvolver um produto que une broadband e broadcast TV sem atrativos ao consumidor, já o aplicativo interativo da Rede Integração não foi colocado no ar e apresenta inúmeros fatores que demonstram que não explorará a interatividade pela ausência de canal de retorno. Este estudo investigou o impacto da interatividade no mercado da radiodifusão e como a convergência tecnológica aponta para a construção de uma nova mídia baseada em displays interativos capazes de oferecer aos consumidores uma nova forma de recepção de conteúdo.