Detecção de metamidofós em solos por métodos ecotoxicológico e cromatografia líquida acoplada à espectrometria de massas sequencial (LC-MS/MS)

Soil contamination by pesticides is an environmental problem that needs to be monitored and avoided. However, the lack of fast, accurate and low cost analytical methods for discovering residual pesticide in complex matrices, such as soil, is a problem still unresolved. This problem needs to be solved before we are able to assess the quality of environmental samples. The intensive use of pesticides has increased since the 60s, because the dependence of their use, causing biological imbalances and promoting resistance and recurrence of high populations of pests and pathogens (upwelling). This has contributed to the appearance of new pests that were previously under natural control. To develop analytical methods that are able to quantify residues pesticide in complex environment. It is still a challenge for many laboratories. The integration of two analytical methods one ecotoxicological and another chemical demonstrates the potential for environmental analysis of methamidophos. The aim of this study was to evaluate an ecotoxicological method as "screening" analytical methamidophos in the soil and perform analytical confirmation in the samples of the concentration of the analyte by chemical method LC-MS/MS In this work we tested two soils: a clayey and sandy, both in contact with the kinetic methamidophos model followed pseudo-second order. The clay soil showed higher absorption of methamidophos and followed the Freundlich model, while the sandy, the Langmuir model. The chemical method was validated LC-MS/MS satisfactory, showing all parameters of linearity, range, precision, accuracy, and sensitivity adequate. In chronic ecotoxicological tests with C. dubia, the NOEC was 4.93 and 3.24 for ng L-1 of methamidophos to elutriate assays of sandy and clay soils, respectively. The method for ecotoxicological levels was more sensitive than LC-MS/MS detection of methamidophos, loamy and sandy soils. However, decreasing the concentration of the standard for analytical methamidophos and adjusting for the validation conditions chemical acquires a limit of quantification (LOQ) in ng L-1, consistent with the provisions of ecotoxicological test. The methods described should be used as an analytical tool for methamidophos in soil, and the ecotoxicological analysis can be used as a "screening" and LC-MS/MS as confirmatory analysis of the analyte molecule, confirming the objectives of this work

O uso de geotecnologias no planejamento ambiental: o plano diretor municipal de Tambaú - SP

A Lei Federal 10.257, de 10 de julho de 2001, regulamentou os artigos 182 e 183 da Constituição Federal de 1988 no que trata da política urbana, especialmente da propriedade, sendo obrigatório para municípios com mais de 20.000 habitantes. Até então, os planos diretores municipais vinham sendo feitos levando em conta apenas as respectivas áreas urbanas, A partir desta lei, tais planos tiveram de levar em conta também as áreas rurais, integrando-as no processo do planejamento municipal sustentado. Analisando-se os planos diretores municipais recentes, elaborados a partir dessas novas diretrizes, nota-se que a maioria ainda enfatiza a área urbana, em detrimento da área rural. O objetivo deste trabalho foi, a partir de um estudo de caso, avaliar e validar metodologias e tecnologias rotineiramente empregadas em Geociências e Ciências Agrárias.

Simplified multiresidue method for the determination of organophosphorus insecticides in olive oil

A simple and rapid method for the determination of 13 organophosphorus insecticides and their metabolites in olive oil by GC is described. The pesticide was extracted from oil with acetonitrile and no cleanup was needed. GC-nitrogen-phosphorus detection response factors of pesticides were affected by solvents and coextractive substances. Pesticides in hexane showed on average higher response factors. Standards were prepared in the residue-free oil extract solubilized in hexane to handle effects of matrix and solvent. The low amount of coextractive substances does not decrease the column efficiency, even after a few hundred analyses. Recovery at three fortification levels (ca. 0.1, 1.0 and 3.0 mg/kg) ranged from 74 to 118%, With coefficients of variation ranging from 1 to 16.

Fast chromatographic determination of polycyclic aromatic hydrocarbons in aerosol samples from sugar cane burning

Sugar cane burning in Brazil causes remarkable amounts of organic compounds to be emitted amongst which the polycyclic aromatic hydrocarbons (PAHs) represent serious health hazards. Therefore, 24-h aerosol samples (< 10 mum aerodynamic diameter) were collected in Araraquara city (São Paulo state) during the harvest season using a Hi-Vol sampler. PAHs were recovered using an Accelerated Solvent Extractor and analyzed by low-pressure gas chromatography-ion trap mass spectrometry (LP-GC-IT-MS). The fully automated extraction process was performed in less than 25 min with a solvent consumption of approximately 20 ml. The use of a deactivated 0.6 m x 0. 10 mm i.d. restrictor coupled to a 10 m wide-bore analytical column allowed most of the 16 PAHs in EPA's priority list to be identified and quantified in only 13 min. Concentrations of PAHs in Nraraquara aerosols ranged between 0.5 and 8.6 ng m(-3). (C) 2003 Elsevier B.V. All rights reserved.

Determination of rice herbicides, their transformation products and clofibric acid using on-line solid-phase extraction followed by liquid chromatography with diode array and atmospheric pressure chemical ionization mass spectrometric detection

A simultaneous method for the trace determination of acidic, neutral herbicides and their transformation products in estuarine waters has been developed through an on-line solid-phase extraction method followed by liquid chromatography with diode array and mass spectrometric detection. An atmospheric pressure chemical ionization (APCI) interface was used in the negative ionization mode after optimization of the main APCI parameters. Limits of detection ranged from 0.1 to 0.02 ng/ml for 50 mi of acidified estuarine waters preconcentrated into polymeric precolumns and using time-scheduled selected ion monitoring mode. Two degradation products of the acidic herbicides (4-chloro-2-methylphenol and 2,4-dichlorophenol) did not show good signal response using APCI-MS at the concentration studied due to the higher fragmentor voltage needed for their determination For molinate and the major degradation product of propanil, 3,4-dichloroaniline, positive ion mode was needed for APCI-MS detection. The proposed method was applied to the determination of herbicides in drainage waters from rice fields of the Delta del Ebro (Spain). During the S-month monitoring of the herbicides, 8-hydroxybentazone and 4-chloro-2-methylphenoxyacetic acid were successively found in those samples. (C) 2000 Elsevier B.V. B.V. All rights reserved.

Diagnóstico ambiental do rio corumbataí em trecho urbano do município de rio claro, SP

The environmental analysis is an important tool used in forecasting and mitigation of environmental problems. Focusing on the occupation of marginal areas of the Corumbataí River in an urban stretch in the city of Rio Claro (SP), this study aimed to gather information on situations of risk, both to the environment and the population, verified in that area. Through field observation and in specific studies, the geological and geotechnical aspects, the characteristics of surface waters and aspects of urbanization were analyzed. The results show that the environmental problems diagnosed are related to lack of planning in the occupation of the area. Moreover, the natural characteristics of the physical environment expose people to risks such as floods and soil slides.

Sistema de informação geográfica - Basins 3.0 na modelagem hidrológica da bacia experimental do Rio Pardo, SP

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Efeitos genéticos e ambientais sobre o intervalo desmame-cio em fêmeas suínas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Zoneamento geoambiental como subsídio aos projetos de reforma agrária. Estudo de caso: assentamento rural Pirituba II (SP)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Proposta para priorização de atividades de remediação no município de São Paulo através da técnica de tomada de decisão multicriterial

Pós-graduação em Geociências e Meio Ambiente - IGCE

Planejamento urbano sustentável: diretrizes de urbanização embasadas nas características geomorfológicas/pedológicas de vertentes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Indicadores de sustentabilidade do cultivo de tilápias-do-nilo (Oreochromis niloticus) em tanques-rede em reservatório tropical

Pós-graduação em Aquicultura - FCAV

Análise ambiental da bacia hidrográfica do Rio Marreco – Oeste do Estado do Paraná

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise integrada de dados no estudo da dinâmica da terra em uma bacia hidrográfica: o caso do Rio Balsinhas-MA

Pós-graduação em Geografia - IGCE