How to confirm C.E.R.A. doping in athletes' blood?

C.E.R.A. (Continuous Erythropoietin Receptor Activator) is a new third-generation erythropoiesis-stimulating agent that has recently been linked with abuse in endurance sports. The anti-doping community rapidly reacted by releasing a high-throughput screening ELISA allowing the detection of C.E.R.A. doping in athletes' blood. In order to return adverse analytical findings, anti-doping laboratories, however, need, as far as possible, to confirm the presence of the drug in athletes' samples through orthogonal methods. This article focuses on the comparison of 2 proposed confirmation assays based on gel electrophoresis that were coupled with a new sample immunopurification method. IEF, the classical method used to target erythropoietin (EPO) and its recombinant analogues in athletes' samples, and SARKOSYL-PAGE were applied to the plasma samples of subjects having received a single injection of C.E.R.A. It was demonstrated that SARKOSYL-PAGE was at least 6 times more sensitive than IEF, with comparable specificity. A longer detection window coupled with easier interpretation criteria led us to recommend the use of SARKOSYL-PAGE to confirm C.E.R.A. presence in athletes' blood.

Atypical EPO profiles in anti-doping analyses

Résumé : Erythropoietin (EPO) is a glycoprotein hormone endogenously produced by the kidney, whose main physiological role is the stimulation of erythropoiesis. Since the beginning of the nineties, recombinant human EPO (rhEPO), a potent anti-anaemia treatment drug, has been manufactured by pharmaceutical industries. However, the erythropoiesis stimulating power of rhEPO was rapidly misused by unscrupulous athletes in order to improve their performances in endurance sports. Endogenous EPO has the same amino-acid backbone as most of recombinant forms; the molecules however differ through their respective glycosylation patterns. This difference constitutes the basis of the usual EPO screening test (IEF) developed in 2000 and still currently used in all anti-doping laboratories of the world. Nowadays, 3 EPO generations have been commercialized. The fight against EPO abuse is a continuous challenge for anti-doping laboratories. The diversity of recombinant EPO forms and the continuous development of new ones considerably confuse the identification of EPO doping. Several facets of this fight were investigated in this work. One of the limiting aspects of doping agents screening is the availability of positive samples. Therefore, 2nd and 3rd generation EPOS, namely NESP and C.E.R.A., were injected to healthy subjects in the frame of pilot clinical studies. These latter allowed to review the current EPO identification criteria defined by the World Anti-Doping Agency (WADA) in the case of NESP and to validate and implement a new assay targeting C.E.R.A. in human serum. Both studies resulted in the determination of the respective detection windows of NESP and C.E.R.A. in biological fluids. Following that, Dynepo, a 1st generation EPO presenting similarities with the endogenous form, was also in the centre of a similar clinical study. Our work aimed to overcome the actual identification criteria, which are not adapted to Dynpeo, and to propose an alternative pattern classification method based on the discriminant analysis of IEF EPO profiles. This method might be validated for other EPO forms in the future. The detection window of this molecule was also determined. Under particular conditions, confounding effects can complicate the identification of EPO in biological matrices. For example, athletes having performed a strenuous physical effort can excrete modified isoforms of endogenous EPO, making it very similar to some recombinant forms. Such phenomena, called effort urines, were reproduced under controlled conditions and, after characterization of effort EPO, an urinary biochemical marker was proposed to unequivocally identify effort urines. It also happens that EPO analyses fail to detect endogenous levels of EPO. Such profiles were thoroughly investigated and potential causes identified. Natural reasons relying on urine properties and test specificity were underlined, but the possible addition of adulterant agents in urine samples was also considered. Therefore, a simple biochemical assay targeting the suspected substances was set up. Our work was based on the characterization of atypical EPO profiles from different origins. Therefore, 3 EPO molecules representing the 3 generations of the drug and 2 confounding effects confusing the results interpretation were studied. These studies resulted in tangible applications for the laboratory, the best example of which being the C.E.R.A. assay, but also in scientific findings allowing to improve our comprehension of EPO doping in sport.

Standardization of metachromatic staining method of myofibrillar ATPase activity of myosin to skeletal striated muscle of mules and donkeys

This study aims at standardizing the pre-incubation and incubation pH and temperature used in the metachromatic staining method of myofibrillar ATPase activity of myosin (mATPase) used for asses and mules. Twenty four donkeys and 10 mules, seven females and three males, were used in the study. From each animal, fragments from the Gluteus medius muscle were collected and percutaneous muscle biopsy was performed using a 6.0-mm Bergström-type needle. In addition to the metachromatic staining method of mATPase, the technique of nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR) was also performed to confirm the histochemical data. The histochemical result of mATPase for acidic pre-incubation (pH=4.50) and alkaline incubation (pH=10.50), at a temperature of 37ºC, yielded the best differentiation of fibers stained with toluidine blue. Muscle fibers were identified according to the following colors: type I (oxidative, light blue), type IIA (oxidative-glycolytic, intermediate blue) and type IIX (glycolytic, dark blue). There are no reports in the literature regarding the characterization and distribution of different types of muscle fibers used by donkeys and mules when performing traction work, cargo transportation, endurance sports (horseback riding) and marching competitions. Therefore, this study is the first report on the standardization of the mATPase technique for donkeys and mules.

Planeamento e orientação de uma equipa de Basquetbol do escalão de juniores "B" ao longo da época 2010/2011

RESUMO:O Basquetebol como desporto global, sendo visualizado e praticado em variados contextos e realidades, deve igualmente perceber-se que existem diferentes formas de vê-lo e treiná-lo. Não existe assim uma forma correta ou incorreta, mas sim várias maneiras de entender o jogo. Neste relatório, no âmbito do Mestrado em Treino Desportivo, expomos a nossa maneira de pensar o jogo bem como as decisões e ações tomadas no processo de treino dos jovens jogadores da equipa de Basquetebol de Juniores “B” Masculinos dos Salesianos Oficinas de São José (OSJ) durante a época desportiva de 2010/2011. A Escola de Basquetebol dos Salesianos OSJ surgiu há cerca de vinte anos, e é um clube integrante e sólido da formação em basquetebol na região de Lisboa. O planeamento foi estruturado num macrociclo, dividido em dois mesociclos, apresentando cada os seus pressupostos e objetivos. O primeiro mesociclo constituído por doze microciclos e o segundo mesociclo por oito microciclos, num total de cento e cinco unidades de treino. A equipa era constituída por treze atletas, com idades compreendidas entre os quinze e os dezanove anos. Relativamente aos jogadores e à equipa consideramos que os objetivos traçados foram alcançados com sucesso, sendo que os jogadores evoluíram significativamente como jogadores e pessoas, e que a equipa atingiu as classificações ambicionadas. Como Treinador considero que foi uma época enriquecedora, que proporcionou vários momentos de reflexão, sendo que estes deverão fazer parte da nossa maneira de estar no desporto, pois só através deles poderemos enriquecer a condução do processo de treino. ABSTRACT: Basketball as a global sport, being viewed and practiced in various contexts and realities, should also realize that are different ways of watching and training it. However, there’s no correct or incorrect way, but many ways of understanding the game. In this report, under the Master of Sports Training, we present our way of thinking the game and the decisions and actions taken in the process of training young basketball players from the Salesianos Oficinas de São José (OSJ) Junior “B” men´s team, during the 2010/2011 sports season. The Salesianos OSJ Basketball School was created about twenty years ago and it’s a strong team member of Young Basketball Training in the Lisbon Region. The team plan was structured in a macrocycle, divided in two mesocycles and each one has its own assumptions and goals. The first mesocycle is composed by twelve microcycles and the second one by eight microcycles, in a total of one hundred and five training sessions. The team is composed by thirteen players, aged between fifteen and nineteen years old. Relating to the players and the team, we believe that the goals were successfully achieved, and the players have evolved meaningly not only as players but also as people, and the team’s coveted standings. As a coach, I think it was an enriching season, which provided many reflective moments and these should be part of our way of being in sport, because only through them we can enrich the process of conducting the training.

Estágio no escalão de iniciados (sub-15) no clube atlético e cultural 2010-2011

RESUMO: O Clube Atlético e Cultural (C.A.C.), apesar de ser relativamente recente, é reconhecido como um Clube Desportivo de formação de atletas de referência na zona de Lisboa. O seu pico de reconhecimento e a sua maior “montra” de formação é sem dúvida o Torneio Internacional da Pontinha (escalão Sub-13), onde é palco de jogos durante 4 dias entre os maiores clubes nacionais, bem como, alguns clubes mundialmente conhecidos. Como prova desse facto, se analisarmos alguns jogadores de elite profissional, têm no seu percurso de formação o C.A.C. como instituição representada. O relatório que de seguida se apresenta, surge no âmbito do estágio de Mestrado em Treino Desportivo e pretende divulgar todas as decisões, acções e reflexões subjacentes ao Treino Desportivo de jovens jogadores. O contexto onde foi realizado foi na equipa de Iniciados “A” (Sub-15), que disputou o Campeonato da Divisão de Honra da Associação Futebol de Lisboa. O relatório encontra-se estruturado do geral para o particular, estando dividido em três tipos de planificação: (1) Planificação Conceptual – na qual abrange uma análise ao Clube em questão, o seu contexto, os seus recursos e os vários constrangimentos. Compreende ainda um estudo prévio de situações anteriores, leia-se, épocas anteriores, bem como, a formação da equipa e dos seus jogadores, em função dos objectivos traçados e assumidos no inicio da época desportiva. Integra também a preparação de todo o período Pré-Competitivo e o recurso a meios de observação e análise visando a melhoria de todo o processo de planeamento do treino. (2) Planificação Estratégica – Esta prevê todas as acções que dizem respeito ao Período Competitivo da equipa, como por exemplo a recolha de dados tanto da nossa equipa como dos adversários, a antecipação dos graus de dificuldade dos nossos adversários, o planeamento mais operacional (Microciclos) e a escolha de estratégias consoante os diferentes adversários e por fim, as reflexões necessárias entre os vários intervenientes no processo. (3) Planificação Táctica – Por fim este tipo de planificação abarca todas as acções, decisões e reacções nos momentos que antecedem o jogo, no momento em que este decorre e ainda após este terminar. Em suma, o relatório pretende expor toda a preparação de uma equipa ao longo de uma época desportiva, visto de uma perspectiva das funções de um treinador principal. ABSTRACT: The Clube Atlético e Cultural (C.A.C.), although relatively recent, is recognized as a reference club in youths training in the Lisbon area. The peak recognition and more “showcase” training ins undoubtedly the Torneio Internacional da Pontinha (Under-13), which is host to games during four days among the biggest national clubs, as well as some world-renowned clubs. As proof thereof, if we look at some elite professional players, have in their educational path C.A.C. represented as an institution. The report that presents itself, appears in the stage of Master of Sports Training and intends to disclose all the decisions, actions and thoughts behind the Sports Training of young players. The report is structured from general to particular, being divided into three types of planning: (1) Conceptual Planning - which includes an analysis on the club in question, its context, its resources and various constraints. It also includes a previous study of past situations like earlier seasons, as well as the formation of the team and its players, according to the objectives and commitments outlined in the beginning of the season. Also includes the preparation of all pre-competition period and the use of means of observation and analysis aimed at improving the entire process of planning the training. (2) Strategic Planning - This provides all the activities that concern the competitive period of the team, such as collecting information both from our team as well as opponents, anticipating the degree of difficulty of our adversaries, the more operational planning (microcycles) and the choice of strategies depending on different opponents and finally the necessary reflections between the various actors in the process. (3) Tactical Planning - Finally this type of planning includes all actions, decisions and reactions in the moments before, during and even after the game. To resume, the report seeks to expose all the preparation of the team throughout a sports season, by the perspective of the functions of a head coach.

A especificidade da escolha da profissão de professor de educação física nos estudantes da Faculdade de Educação Física e Desporto da Universidade Lusófona de Humanidades e Tecnologias

RESUMO: O presente estudo pretende distinguir a especificidade das referências dos estudantes dos três ramos da licenciatura de Educação Física da Universidade Lusófona de Humanidades e Tecnologias (ULHT), para a escolha do curso e da profissão de professor de educação física, bem como comparar a especificidade das referências dos estudantes dentro dos cinco anos constituintes da formação inicial. Pretende-se ainda caracterizar as representações dos estudantes de Educação Física e Desporto Escolar (EFDE), a frequentar o 3º ano da Licenciatura e o 1º e 2º ano do Mestrado, sobre as orientações da formação recebida. Juntos dos estudantes do 5º ano, pretende-se caracterizar as representações que estes tiveram sobre o apoio e supervisão dos Orientadores de Estágio, bem como caracterizar a auto-avaliação que realizam sobre os seus resultados profissionais no relacionamento com os alunos, durante o ano de estágio pedagógico. Participaram neste estudo 450 estudantes da Faculdade de Educação Física e Desporto, da Universidade Lusófona de Humanidades e Tecnologias. Tendo por base o estudo “Motivação para a Profissão Docente”, de 1996, do Professor Doutor Saul Neves de Jesus e a metodologia por ele realizada, foi aplicado o mesmo tipo de estudo a outra amostra tentando perceber de que forma é que esta se manifestava, tendo em conta os objectivos delineados. O instrumento utilizado, originalmente construído e aplicado por Jesus (1996), é um inquérito por questionário, composto por 44 itens com resposta em escala tipo likert. De forma a avaliar as problemáticas em estudo, estes itens encontravam-se divididos em quatro dimensões: motivação inicial para a profissão docente, modelo de formação educacional, apoio no estágio pedagógico e resultados profissionais obtidos no ano de estágio pedagógico. Os estudantes do ramo de EDFE mostram estar mais focados nos aspectos nucleares a ter em conta aquando da escolha da profissão de professor de educação física do que os estudantes dos ramos de Exercício e Bem-Estar (EBE) e Treino Desportivo (TD). Dos três ramos em análise, os estudantes de TD são os que mais vincadamente marcam a posição de não quererem ser professores de educação física. Os estudantes de EFDE, a frequentar o 3º e último ano da Licenciatura e o 1º e 2º ano da Mestrado (descritos como 4º e 5º ano), avaliam positivamente o modelo de formação educacional recebido. Dos três anos em análise, são os alunos do 3º ano que obtiveram a média mais alta na pontuação de sete dos onze itens que compunham esta dimensão. Os estudantes do 5º ano têm uma representação bastante positiva acerca do apoio prestado pelos seus orientadores de estágio, bem como realizam uma auto-avaliação bastante positiva no que diz respeito ao relacionamento com os seus alunos e aos objectivos profissionais daí inerentes durante o ano de estágio pedagógico. ABSTRACT: The present study aims to distinguish the specificity of the references of the students in the three branches, of the Physical Education degree on Universidade Lusófona de Humanidades e Tecnologias (ULHT), for choosing the course and the profession of physical education teacher, and to compare the specificity of the references of students within five years of initial constituents. Another objective is to characterize the representations of the students of Physical Education and School Sport (EFDE), to attend the 3rd year of Bachelor and 1st and 2nd year of the Masters on the guidelines of the education received. Have participated in this study 450 students from the Faculty of Physical Education and Sport, of Universidade Lusófona de Humanidades e Tecnologias. Based on the study "Motivation for the Teaching Profession", 1996, by Professor Saul Neves de Jesus and the methodology he performs, we applied the same type of study to another sample trying to understand how this is manifested, taking into account the objectives outlined. The instrument used, originally built and used by Jesus (1996), is a questionnaire composed of 44 items with Likert scale response. In order to assess the problem under study, these items were divided into four dimensions: initial motivation for the teaching profession, type of educational background, support in teaching practice and professional results obtained in the year of teaching practice. Students of the branch EDFE shown to be more focused on core aspects to consider when choosing the profession of physical education teacher compared to students from branches of Exercise and Wellness (SBS) and Sports Training (DT). Of the three branches in analysis, students of TD are the most that distinctly mark the position of not wanting to be physical education teachers. EFDE students, to attend the 3rd and final year of the Bachelor and the 1st and 2nd year of Master (described as 4th and 5th year), positively evaluate the educational model received. Of the three years under review the third grade students are the ones who achieved the highest average score in seven of the eleven items that made up this dimension. Students in fifth grade have a very positive representation about the support provided by their advisors stage and perform a self-assessment very positive with regard to the relationship with their students and professional goals there inherent during stage pedagogical.

Kön, kroppsbild och dysfunktionella tankar : en sambandsstudie

Body esteem is the affective aspect of body image, which is shaped by social experience. Compared with men, women have a more negative body image, which is more frequently correlated with depression and dysfunctional thoughts, especially in the case of eating disorders. The purpose of the present study was to examine gender differences in body esteem and its subcategories, and to find out whether there exists a stronger link between negative body esteem and higher levels of dysfunctional thoughts in women. The relationship between body esteem, dysfunctional thoughts and mental illness was examined. Participants were 73 college students doing social sciences and sports training educations. Body esteem questionnaire and dysfunctional thoughts questionnaire as well as a self made questionnaire on mental illness were used. Results showed that women had a more negative body esteem compared to men, especially considering weight. In women, there was a medium-strong negative relation between body esteem and dysfunctional thoughts. The results indicate that the norms for female body ideal that abound in the Western society have a negative affect on women's thinking and body image.Key words: Gender, body esteem, dysfunctional thoughts, mental illness.

Avaliação dos efeitos do treinamento no período preparatório em atletas profissionais de futebol

O objetivo do presente estudo foi avaliar os efeitos do treinamento no período preparatório em atletas profissionais de futebol. Participam 17 atletas, com idade acima de 18 anos que disputaram a terceira divisão do campeonato paulista, em 2009. O programa de treinamento predominante foi o tipo neuromuscular e por meio dos testes de sentar e alcançar, salto sêxtuplo, salto vertical e velocidade de deslocamento em 30m os atletas foram avaliados antes do início do programa de treinamento (M1) e após sete semanas (M2). Os dados coletados foram armazenados em banco computacional, produzindo-se informações no plano descritivo (medidas de centralidade e dispersão) e, no inferencial, Teste t de Student e Anova One-way. Os principais resultados apontam para melhora da flexibilidade, força explosiva e rápida, e sensível diminuição na velocidade de deslocamento em 30 metros.

Etapas e volume de treinamento das ginastas Brasileiras participantes de Jogos Olímpicos (1980-2004)

Results from Brazilian female Artistic Gymnastics improved over the past 10 years at an international level of competition. This study aimed at describing career development process in sports through a qualitative research resorting to the Oral History method. Ten Brazilian gymnast players in Olympic Games from 1980 to 2004 participated in the study. The information they provided allowed for the identification of specific variables present in their long term preparation: structure and organization of their practice, duration of the preliminary preparation phases, volume of daily practice in different phases of training, and length of time for the achievement of better results. © FTCD/CIDESD.

Efeito do exercício aeróbio prévio realizado em diferentes cadências de pedalada sobre o número máximo de repetições na musculação e a capacidade de saltos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência das concentrações de marcadores bioquímicos de supertreinamento sobre as performances aeróbia e anaeróbia durante periodização em natação

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Protocolos de treinamento aeróbio intervalado e da periodização para natação com ratos

Pós-graduação em Ciências da Motricidade - IBRC

Efeitos de quatro modelos de treinamento experimental de natação sobre biomarcadores de adaptação e capacidades aeróbia e anaeróbia em ratos wistar

Pós-graduação em Ciências da Motricidade - IBRC

A noção de espaço na prática desportiva: um estudo de casos múltiplos com base na epistemologia genética com praticantes de futsal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Standardization of metachromatic staining method of myofibrillar ATPase activity of myosin to skeletal striated muscle of mules and donkeys

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)