Setting up a bioreactor for recombinant protein production in yeast

Scale-up from shake flasks to bioreactors allows for the more reproducible, high-yielding production of recombinant proteins in yeast. The ability to control growth conditions through real-time monitoring facilitates further optimization of the process. The setup of a 3-L stirred-tank bioreactor for such an application is described. © 2012 Springer Science+business Media, LLC.

Left to right: Dan Bond and Melissa Romigh. Setting up a flume panel to measure nutrient / sediment fluxed in a mangrove forest at SRS-6, Shark River Slough

This material is based upon work supported by the National Science Foundation through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements #DBI-0620409 and #DEB-9910514. This image is made available for non-commercial or educational use only.

Setting up a network of agrometeorological stations in East Timor

A developed and sustainable agriculture requires a permanent and reliable monitoring of climatic/ meteorological elements in (agro) meteorological stations which should be located close to agricultural, silvicultural or pastoral activities. An adequate network of meteorological stations is then a necessary condition to support innovation and development in any country. Developing countries, mainly those with a history of frequent conflicts, presents deficient number of weather stations, often poorly composed and improperly distributed within their territories, and without a regular operation that allows continuity of records for a sufficiently long period of time. The objective of this work was to build a network of meteorological and agro-meteorological stations in East Timor. To achieve this goal, the number and location of pre-existing stations, their structure and composition (number and type of sensors, communication system,… ), the administrative division of the country and the available agro-ecological zoning, the agricultural and forestry practices in the country, the existing centres for the agricultural research and the history of the weathers records were taken into account. Several troubles were found (some of the automatic stations were assembled incorrectly, others stations duplicated information regarding the same agricultural area, vast areas with relevant agro-ecological representativeness were not monitored …). It was proposed the elimination of 11 existing stations, the relocation of 7 new stations in places not covered until then, the automation of 3 manual meteorological stations. Two networks were then purposed, a major with 15 agro-meteorological stations (all automatized) and one other secondary composed by 32 weather stations (only two were manual). The set of the 47 stations corresponded to a density of 329 km2/station. The flexibility in the composition of each of the networks was safeguarded and intends to respond effectively to any substantive change in the conditions in a country in constant change. It was also discussed the national coverage by these networks under a “management concept for weather stations”.

“O sonho do museólogo”. A exposição: desafio para uma nova linguagem museográfica

Julgamos que é um dado adquirido que na entrada dos museus existe um número excessivo de proibições e, essa característica nas instituições museológica sempre foi algo que me incomodou profundamente. Visitando durante um final-de-semana vários museus, tornou-se assim mais evidente que em todos eles havia logo na porta principal um cartaz que enumerava tudo que o visitante não podia fazer, entre a listagem de proibições estavam: “não correr, não fotografar, não comer dentro das salas de exposições, não tocar, não gritar”. Após o sexto museu visitado, comentei com um amigo que o ideal de todo museólogo era colocar o visitante dentro de uma vitrina, só assim conseguiriam proteger seus acervos.

Automatic selection of tidal volume, respiratory frequency and minute ventilation in intubated ICU patients as start up procedure for closed-loop controlled ventilation.

OBJECTIVE: Before a patient can be connected to a mechanical ventilator, the controls of the apparatus need to be set up appropriately. Today, this is done by the intensive care professional. With the advent of closed loop controlled mechanical ventilation, methods will be needed to select appropriate start up settings automatically. The objective of our study was to test such a computerized method which could eventually be used as a start-up procedure (first 5-10 minutes of ventilation) for closed-loop controlled ventilation. DESIGN: Prospective Study. SETTINGS: ICU's in two adult and one children's hospital. PATIENTS: 25 critically ill adult patients (age > or = 15 y) and 17 critically ill children selected at random were studied. INTERVENTIONS: To stimulate 'initial connection', the patients were disconnected from their ventilator and transiently connected to a modified Hamilton AMADEUS ventilator for maximally one minute. During that time they were ventilated with a fixed and standardized breath pattern (Test Breaths) based on pressure controlled synchronized intermittent mandatory ventilation (PCSIMV). MEASUREMENTS AND MAIN RESULTS: Measurements of airway flow, airway pressure and instantaneous CO2 concentration using a mainstream CO2 analyzer were made at the mouth during application of the Test-Breaths. Test-Breaths were analyzed in terms of tidal volume, expiratory time constant and series dead space. Using this data an initial ventilation pattern consisting of respiratory frequency and tidal volume was calculated. This ventilation pattern was compared to the one measured prior to the onset of the study using a two-tailed paired t-test. Additionally, it was compared to a conventional method for setting up ventilators. The computer-proposed ventilation pattern did not differ significantly from the actual pattern (p > 0.05), while the conventional method did. However the scatter was large and in 6 cases deviations in the minute ventilation of more than 50% were observed. CONCLUSIONS: The analysis of standardized Test Breaths allows automatic determination of an initial ventilation pattern for intubated ICU patients. While this pattern does not seem to be superior to the one chosen by the conventional method, it is derived fully automatically and without need for manual patient data entry such as weight or height. This makes the method potentially useful as a start up procedure for closed-loop controlled ventilation.

Planning and ramping up a new SW Testing site

During recent years, mobile phone markets have changed significantly. Asian markets have become vital for the manufacturers with their millions of end users and multiple major mobile network operators. This has resulted in software development as global companies have research and development sites running in multiple locations, including Asia. The reasons behind this are not only in reducing labor costs but also in capitalizing on the local knowledge and knowhow. A ramp-up site has multiple effects in the software development and software release activities. This thesis focuses on representing the importance of software testing as part of software development process and highlighting issues that need to be considered during ramp-up activities. In addition this work tries to emphasize the importance of communication between parties and information gathering prior to setting up the ramp-up site. The output of this thesis was successful software testing site ramp-up within the set time limits. The quality of software testing work was assured and the ramp-up -project requirements were achieved.

Práticas corporais alternativas e educação física : entre a formação e a intervenção

Universidade Estadual de Campinas. Faculdade de Educação Física