Linked Data for sharing, discovery and re-use of Language Resources at a Web scale

Conferencia por invitación, impartida el 31 d mayo de 2014 en el Workshop on Language Technology Service Platforms: Synergies, Standards, Sharing at LREC2014

Gene discovery and gene function assignment in filamentous fungi

Filamentous fungi are a large group of diverse and economically important microorganisms. Large-scale gene disruption strategies developed in budding yeast are not applicable to these organisms because of their larger genomes and lower rate of targeted integration (TI) during transformation. We developed transposon-arrayed gene knockouts (TAGKO) to discover genes and simultaneously create gene disruption cassettes for subsequent transformation and mutant analysis. Transposons carrying a bacterial and fungal drug resistance marker are used to mutagenize individual cosmids or entire libraries in vitro. Cosmids are annotated by DNA sequence analysis at the transposon insertion sites, and cosmid inserts are liberated to direct insertional mutagenesis events in the genome. Based on saturation analysis of a cosmid insert and insertions in a fungal cosmid library, we show that TAGKO can be used to rapidly identify and mutate genes. We further show that insertions can create alterations in gene expression, and we have used this approach to investigate an amino acid oxidation pathway in two important fungal phytopathogens.

The synthesis, discovery, and development of a highly promising class of microtubule stabilization agents: Curative effects of desoxyepothilones B and F against human tumor xenografts in nude mice

We have evaluated two synthetic epothilone analogues lacking the 12,13-epoxide functionality, 12,13-desoxyepothilone B (dEpoB), and 12,13-desoxyepothilone F (dEpoF). The concentrations required for 50% growth inhibition (IC50) for a variety of anticancer agents were measured in CCRF-CEM/VBL1000 cells (2,048-fold resistance to vinblastine). By using dEpoB, dEpoF, aza-EpoB, and paclitaxel, the IC50 values were 0.029, 0.092, 2.99, and 5.17 μM, respectively. These values represent 4-, 33.5-, 1,423- and 3,133-fold resistance, respectively, when compared with the corresponding IC50 in the parent [nonmultiple drug-resistant (MDR)] CCRF-CEM cells. We then produced MDR human lung carcinoma A549 cells by continuous exposure of the tumor cells to sublethal concentrations of dEpoB (1.8 yr), vinblastine (1.2 yr), and paclitaxel (1.8 yr). This continued exposure led to the development of 2.1-, 4,848-, and 2,553-fold resistance to each drug, respectively. The therapeutic effect of dEpoB and paclitaxel was also compared in vivo in a mouse model by using various tumor xenografts. dEpoB is much more effective in reducing tumor sizes in all MDR tumors tested. Analysis of dEpoF, an analog possessing greater aqueous solubility than dEpoB, showed curative effects similar to dEpoB against K562, CCRF-CEM, and MX-1 xenografts. These results indicate that dEpoB and dEpoF are efficacious antitumor agents with both a broad chemotherapeutic spectrum and wide safety margins.

The discovery and conquest of Mexico, 1517-1521.

"Some extracts from the letters of Hernando Cortés are added to make clear the topography of the siege of the city."

Relation of the discovery and conquest of the kingdoms of Peru /

Reprint. Originally published: New York : The Cortes Society, 1921, in series: Documents and narratives concerning the discovery and conquest of Latin America ; no. 4, v. 1.

A catalogue of books relating to the discovery and early history of North and South America : forming a part of the library of E.D. Church /

Edition of 100 copies.

Wells's natural philosophy : for the use of schools, academies, and private students. Introducing the latest results of scientific discovery and research; arranged with special reference to the practical application of physical science to the arts and the experiences of every-day life. With three hundred and seventy-five engravings /

Mode of access: Internet.

History of Louisiana, from its first discovery and settlement to the present time.

Mode of access: Internet.

Discovery and exploration of the Mississippi Valley,

Mode of access: Internet.

Questioned document problems, the discovery and proof of the facts.

Bibliography: p. [541]-549.

The knights of the frozen sea. A narrative of Arctic discovery and adventure.

Mode of access: Internet.

Narrative of discovery and adventure in Africa, from the earliest ages to the present time: with illustrations of the geology, mineralogy and zoology.

Added t.-p., illus.

Notes on Cuba : containing an account of its discovery and early history ; a description of the face of the country, its population, resources, and wealth ; its institutions, and the manners and customs of its inhabitants ; with directions to travellers visiting the island /

Mode of access: Internet.

By Nile and Euphrates ; a record of discovery and adventure,

Mode of access: Internet.

The Retrospect of practical medicine and surgery, being a half-yearly journal containing a retrospective view of every discovery and practical improvement in the medical sciences ...

Editors: 1840- William Braithwaite.-18 James Braithwaite, E.F. Trevelyan.