Morphology, sex ratio and gene expression of Day 14 in vivo and in vitro bovine embryos

The present study was designed to compare Day 14 bovine embryos that were produced entirely in vitro using the post-hatching development (PHD) system with in vivo-derived embryos without or with transient PHD culture from Day 7 to Day 14. Embryos on Day 14 were used for sex determination and gene expression analysis of PLAC8, KRT8, CD9, SLC2A1, SLC2A3, PGK1, HSF1, MNSOD, HSP70 and IFNT using real-time quantitative (q) polymerase chain reaction (PCR). First, Day 7 in vivo-and in vitro-produced embryos were subjected to the PHD system. A higher rate of survival was observed for in vitro embryos on Day 14. Comparing Day 14 embryos produced completely in vivo or completely in vitro revealed that the mean size of the former group was greater than that of the latter (10.29±1.83 vs 2.68±0.33mm, respectively). Expression of the HSP70 and SLC2A1 genes was down-and upregulated, respectively, in the in vitro embryos. The present study shows that in vitro embryos cultured in the PHD system are smaller than in vivo embryos and that of the 10 genes analysed, only two were differentially expressed between the two groups. These findings indicate that, owing to the poor survival rate, the PHD system is not reliable for evaluation of in vitro embryo quality. © 2013 CSIRO.

Reestructuración y revitalización de las Naciones Unidas en las esferas económica y social: información actualizada sobre sus consecuencias para la Comisión Económica para América Latina y el Caribe = Restructuring and revitalization of the United Nations in the economic and social fields: an update on its impact on the Economic Commission for Latin America and the Caribbean = Restructuration et revitalisation de l'Organisation des Nations Unies dans les domaines économique et social: information actualisée sur les repercussions pour la CEPALC

Incluye Bibliografía

Descentralización de las actividades económicas y sociales y robustecimiento de las comisiones económicas regionales = Decentralization of the United Nations Economic and Social activities and strengthening of the Regional Economic Commissions

Incluye Bibliografía

Descentralización de las actividades económicas y sociales de las NU: las nuevas funciones de las comisiones regionales en la cooperación regional e interregional = Decentralization of the economic and social activities of the UN: the new functions of the regional commissions in regional and interregional co-operation

Incluye Bibliografía

Análise teórica e experimental do comportamento de vigas de alvenaria estrutural armada sujeitas à flexão simples

Pós-graduação em Engenharia Civil - FEIS

Visualização da informação colaborativa por meio de um ambiente multiprojetado e dispositivos móveis

Pós-graduação em Ciência da Computação - IBILCE

Genetically targeted cell disruption in Caenorhabditis elegans

The elimination of identified cells is a powerful tool for investigating development and system function. Here we report on genetically mediated cell disruption effected by the toxic Caenorhabditis elegans mec-4(d) allele. We found that ectopic expression of mec-4(d) in the nematode causes dysfunction of a wide range of nerve, muscle, and hypodermal cells. mec-4(d)-mediated toxicity is dependent on the activity of a second gene, mec-6, rendering cell disruption conditionally dependent on genetic background. We describe a set of mec-4(d) vectors that facilitate construction of cell-specific disruption reagents and note that genetic cell disruption can be used for functional analyses of specific neurons or neuronal classes, for confirmation of neuronal circuitry, for generation of nematode populations lacking defined classes of functional cells, and for genetic screens. We suggest that mec-4(d) and/or related genes may be effective general tools for cell inactivation that could be used toward similar purposes in higher organisms.

Approaches and Strategy for Cancer Research and Surveillance Data: Integration, Information Pipeline, Data Models, and Informatics Opportunities

Thesis (Ph.D.)--University of Washington, 2016-04

Advanced techniques for the improvement of optical transmission systems

This thesis presents the experimental investigation into two novel techniques which can be incorporated into current optical systems. These techniques have the capability to improve the performance of transmission and the recovery of the transmitted signal at the receiver. The experimental objectives are described and the results for each technique are presented in two sections: The first experimental section is on work related to Ultra-long Raman Fibre lasers (ULRFLs). The fibre lasers have become an important research topic in recent years due to the significant improvement they give over lumped Raman amplification and their potential use in the development of system with large bandwidths and very low losses. The experiments involved the use of ASK and DPSK modulation types over a distance of 240km and DPSK over a distance of 320km. These results are compared to the current state of-the-art and against other types of ultra-long transmission amplification techniques. The second technique investigated involves asymmetrical, or offset, filtering. This technique is important because it deals with the strong filtering regimes that are a part of optical systems and networks in modern high-speed communications. It allows the improvement of the received signal by offsetting the central frequency of a filter after the output of a Delay Line Interferometer (DLI), which induces significant improvement in BER and/or Qvalues at the receiver and therefore an increase in signal quality. The experimental results are then concluded against the objectives of the experimental work and potential future work discussed.

Rudolf Lassahn/Birgit Ofenbach (Hrsg.): Bildung in Europa. Frankfurt a. M./Bern: Lang 1993. 162 S. [...] [Sammelrezension]

Sammelrezension von: 1. Rudolf Lassahn/Birgit Ofenbach (Hrsg.): Bildung in Europa. Frankfurt a. M./Bern: Lang 1993. 162 S. 2. Walter Hornstein/Gerd Mutz unter Mitarbeit von Irene Kühnlein und Angelika Poferl: Die europäische Einigung als gesellschaftlicher Prozeß. Soziale Problemlagen, Partizipation und kulturelle Transformation. Baden-Baden: Nomos 1993. 275 S.

Oskar Anweiler/Hans-Jürgen Fuchs/Martina Dorner/Eberhard Petermann (Hrsg.): Bildungspolitik in Deutschland 1945-1990. Ein historisch-vergleichender Quellenband. Opladen: Leske + Budrich 1992 [Rezension]

Rezension von: Oskar Anweiler/Hans-Jürgen Fuchs/Martina Dorner/Eberhard Petermann (Hrsg.): Bildungspolitik in Deutschland 1945-1990. Ein historisch-vergleichender Quellenband. Opladen: Leske + Budrich 1992, 574 S.

A charged-particle microbeam .1. Development of an experimental system for targeting cells individually with counted particles

Charged-particle microbeams provide a unique opportunity to control precisely, the dose to individual cells and the localization of dose within the cell. The Gray Laboratory is now routinely operating a charged-particle microbeam capable of delivering targeted and counted particles to individual cells, at a dose-rate sufficient to permit a number of single-cell assays of radiation damage to be implemented. By this means, it is possible to study a number of important radiobiological processes in ways that cannot be achieved using conventional methods. This report describes the rationale, development and current capabilities of the Gray Laboratory microbeam.