Microfluidic electronic tongue

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Emprego de técnica cirúrgica e materiais adesivos diretos no fechamento de diastema

The following is a clinical case report of a patient whose main complaint was the presence of a generalized spacing in the anterior maxillary segment. After meticulous clinical analysis and discussions of the clinical procedures to be adopted, a labial frenectomy was our first choice, so that we could reduce the diastemas directly using composite resin. It was observed that the association of clinical and restorative procedures was capable of giving back shape, function and dental aesthetics, allowing to the young patient the satisfaction of smiling without any fear.

Lupus anticoagulant: a marker for stroke and venous thrombosis in primary Sjogren's syndrome

Antiphospholipid antibodies (aPL) and antiphospholipid syndrome (APS) have been described in primary Sjogren's syndrome (pSS) with controversial findings regarding aPL prevalence and their association with thrombotic events. We evaluated 100 consecutive pSS patients (American-European criteria) and 89 age-gender-ethnicity-matched healthy controls for IgG/IgM anticardiolipin (aCL), IgG/IgM anti-beta2-glycoprotein-I (a beta 2GPI), and lupus anticoagulant (LA) (positivity according to APS Sydney's criteria). Clinical analysis followed standardized interview and physical examination assessing thrombotic and nonthrombotic APS manifestations and thrombosis risk factors. aPLs were detected in 16 % patients and 5.6 % controls (p = 0.035). LA was the most common aPL in patients (9 %), followed by a beta 2GPI (5 %) and aCL (4 %). Thrombotic events occurred in five patients [stroke in two, myocardial infarction in one and deep-vein thrombosis (DVT) in four], but in none of controls (p = 0.061). Mean age at time of stroke was 35 years. Three patients with thrombotic events (including the two with stroke) had APS (Sydney's criteria) and were positive exclusively for LA. Comparison of patients with (n = 16) and without (n = 84) aPL revealed similar mean age, female predominance, and ethnicity (p > =0.387). Frequencies of livedo reticularis (25 vs. 4.8 %, p = 0.021), stroke (12.5 vs. 0 %, p = 0.024), and DVT (18.8 vs. 1.2 %, p = 0.013) were significantly higher in APL + patients. Conversely, frequencies of hypertension, dyslipidemia, diabetes, obesity, smoking, sedentarism, and hormonal contraception were similar in patients with or without aPL (p a parts per thousand yenaEuro parts per thousand 0.253). Our study identified LA as an important marker for APS in pSS, particularly for stroke in young patients, warranting routine evaluation of these antibodies and rigorous intervention in modifiable risk factors.

The impact of healing time before loading on orthodontic mini-implant stability: a histomophometric study in minipigs

Objective: To evaluate healing time before loading, areas compression and tension and location of insertion on mini-implant stability. Design: Six minipigs were used. Each animal received 3 mini-implants in each quadrant: 1 mini-implant was used as an unloaded control (G1, n = 24); the other 2 were loaded with 150 g-force at three time intervals (G2: immediate loading, G3: after 15 days and G4: after 30 days), with 16 mini-implant in each experimental group. After 120 days, tissue blocks of the areas of interest were harvested. Clinical analysis (exact Fisher test) determined the survival rate. Histological analysis (Kontron KS 300TM, Zeiss) quantified the fractional bone-toimplant contact (%BIC) and bone area (%BA) at each healing time point, areas of interest, and insertion site (ANOVA and t tests for dependent and independent samples). Results: The mini-implant survival rates were G1: 71%, G2: 50%, G3: 75% and G4: 63%, with no statistical differences between them. The groups presented similar %BIC and %BA. There were no differences between the compression and tension sides or maxillary and mandibular insertion sites. Conclusions: These results suggest that low-intensity immediate or early orthodontic loading does not affect mini-implant stability, because similar histomorphometric results were observed for all the groups, with partial osseointegration of the mini-implants present.

Emergence of linezolid-resistant Staphylococcus aureus after prolonged treatment of cystic fibrosis patients in Cleveland, Ohio

Linezolid (LZD)-resistant Staphylococcus aureus (LRSA) isolates were monitored from 2000 to 2009 in Cleveland, OH. LRSA first emerged in 2004 only in cystic fibrosis (CF) patients, with 11 LRSA-infected CF patients being identified by 2009. LRSA was isolated from 8 of 77 CF patients with S. aureus respiratory tract infection treated with LZD from 2000 to 2006. Analysis of clinical data showed that the 8 CF patients with LRSA received more LZD courses (18.8 versus 5.9; P = 0.001) for a longer duration (546.5 versus 211.9 days; P < 0.001) and had extended periods of exposure to LZD (83.1 versus 30.1 days/year; P < 0.001) than the 69 with LZD-susceptible isolates. Five LRSA isolates included in the clinical analysis (2000 to 2006) and three collected in 2009 were available for molecular studies. Genotyping by repetitive extrapalindromic PCR and pulsed-field gel electrophoresis revealed that seven of these eight LRSA strains from unique patients were genetically similar. By multilocus sequence typing, all LRSA isolates were included in clonal complex 5 (seven of sequence type 5 [ST5] and one of ST1788, a new single-locus variant of ST5). However, seven different variants were identified by spa typing. According to the Escherichia coli numbering system, seven LRSA isolates contained a G2576T mutation (G2603T, S. aureus numbering) in one to four of the five copies of domain V of the 23S rRNA genes. One strain also contained a mutation (C2461T, E. coli numbering) not previously reported. Two strains, including one without domain V mutations, possessed single amino acid substitutions (Gly152Asp or Gly139Arg) in the ribosomal protein L3 of the peptidyltransferase center, substitutions not previously reported in clinical isolates. Emergence of LRSA is a serious concern for CF patients who undergo prolonged courses of LZD therapy.

Reconstruction of femoro-acetabular offsets using a short-stem

PURPOSE Despite the fact that new and modern short-stems allow bone sparing and saving of soft-tissue and muscles, we still face the challenge of anatomically reconstructing the femoro-acetabular offset and leg length. Therefore a radiological and clinical analysis of a short-stem reconstruction of the femoro-acetabular offset and leg length was performed. METHODS Using an antero-lateral approach, the optimys short-stem (Mathys Ltd, Bettlach, Switzerland) was implanted in 114 consecutive patients in combination with a cementless cup (Fitmore, Zimmer, Indiana, USA; vitamys RM Pressfit, Mathys Ltd, Bettlach, Switzerland). Pre- and postoperative X-rays were done in a standardized technique. In order to better analyse and compare X-ray data a special double coordinate system was developed for measuring femoral- and acetabular offset. Harris hip score was assessed before and six weeks after surgery. Visual analogue scale (VAS) satisfaction, leg length difference and the existence of gluteal muscle insufficiency were also examined. RESULTS Postoperative femoral offset was significantly increased by a mean of 5.8 mm. At the same time cup implantation significantly decreased the acetabular offset by a mean of 3.7 mm, which resulted in an increased combined femoro-acetabular offset of 2.1 mm. Postoperatively, 81.7 % of patients presented with equal leg length. The maximum discrepancy was 10 mm. Clinically, there were no signs of gluteal insufficiency. No luxation occurred during hospitalization. The Harris hip score improved from 47.3 before to 90.1 points already at six weeks after surgery while the mean VAS satisfaction was 9.1. CONCLUSION The analysis showed that loss of femoro-acetabular offset can be reduced with an appropriate stem design. Consequently, a good reconstruction of anatomy and leg length can be achieved. In the early postoperative stage the clinical results are excellent.

Usability and Acceptability of ASSESS MS: Assessment of Motor Dysfunction in Multiple Sclerosis Using Depth-Sensing Computer Vision

Background: Sensor-based recordings of human movements are becoming increasingly important for the assessment of motor symptoms in neurological disorders beyond rehabilitative purposes. ASSESS MS is a movement recording and analysis system being developed to automate the classification of motor dysfunction in patients with multiple sclerosis (MS) using depth-sensing computer vision. It aims to provide a more consistent and finer-grained measurement of motor dysfunction than currently possible. Objective: To test the usability and acceptability of ASSESS MS with health professionals and patients with MS. Methods: A prospective, mixed-methods study was carried out at 3 centers. After a 1-hour training session, a convenience sample of 12 health professionals (6 neurologists and 6 nurses) used ASSESS MS to capture recordings of standardized movements performed by 51 volunteer patients. Metrics for effectiveness, efficiency, and acceptability were defined and used to analyze data captured by ASSESS MS, video recordings of each examination, feedback questionnaires, and follow-up interviews. Results: All health professionals were able to complete recordings using ASSESS MS, achieving high levels of standardization on 3 of 4 metrics (movement performance, lateral positioning, and clear camera view but not distance positioning). Results were unaffected by patients’ level of physical or cognitive disability. ASSESS MS was perceived as easy to use by both patients and health professionals with high scores on the Likert-scale questions and positive interview commentary. ASSESS MS was highly acceptable to patients on all dimensions considered, including attitudes to future use, interaction (with health professionals), and overall perceptions of ASSESS MS. Health professionals also accepted ASSESS MS, but with greater ambivalence arising from the need to alter patient interaction styles. There was little variation in results across participating centers, and no differences between neurologists and nurses. Conclusions: In typical clinical settings, ASSESS MS is usable and acceptable to both patients and health professionals, generating data of a quality suitable for clinical analysis. An iterative design process appears to have been successful in accounting for factors that permit ASSESS MS to be used by a range of health professionals in new settings with minimal training. The study shows the potential of shifting ubiquitous sensing technologies from research into the clinic through a design approach that gives appropriate attention to the clinic environment.

Development and Characterization of an in vitro Four-species Anaerobic Dental Biofilm Model

Dental caries is the most common chronic disease worldwide. It is characterized by the demineralization of tooth enamel caused by acid produced by cariogenic dental bacteria growing on tooth surfaces, termed bacterial biofilms. Cariogenesis is a complex biological process that is influence by multiple factors and is not attributed to a sole causative agent. Instead, caries is associated with multispecies microbial biofilm communities composed of some bacterial species that directly influence the development of a caries lesion and other species that are seemingly benign but must contribute to the community in an uncharacterized way. Clinical analysis of dental caries and its microbial populations is challenging due to many factors including low sensitivity of clinical measurement tools, variability in saliva chemistry, and variation in the microbiota. Our laboratory has developed an in vitro anaerobic biofilm model for dental carries to facilitate both clinical and basic research-based analyses of the multispecies dynamics and individual factors that contribute to cariogenicity. The rational for development of this system was to improve upon the current models that lack key elements. This model places an emphasis on physiological relevance and ease of maintenance and reproducibility. The uniqueness of the model is based on integrating four critical elements: 1) a biofilm community composed of four distinct and representative species typically associated with dental caries, 2) a semi-defined synthetic growth medium designed to mimic saliva, 3) physiologically relevant biofilm growth substrates, and 4) a novel biofilm reactor device designed to facilitate the maintenance and analysis. Specifically, human tooth sections or hydroxyapatite discs embedded into poly(methyl methacrylate) (PMMA) discs are incubated for an initial 24 hr in a static inverted removable substrate (SIRS) biofilm reactor at 37°C under anaerobic conditions in artificial saliva (CAMM) without sucrose in the presence of 1 X 106 cells/ml of each Actinomyces odontolyticus, Fusobacterium nucleatum, Streptococcus mutans, and Veillonella dispar. During days 2 and 3 the samples are maintained continually in CAMM with various exposures to 0.2% sucrose; all of the discs are transferred into fresh medium every 24 hr. To validate that this model is an appropriate in vitro representation of a caries-associated multispecies biofilm, research aims were designed to test the following overarching hypothesis: an in vitro anaerobic biofilm composed of four species (S. mutans, V. dispar, A. odontolyticus, and F. nucleatum) will form a stable biofilm with a community profile that changes in response to environmental conditions and exhibits a cariogenic potential. For these experiments the biofilms as described above were exposed on days 2 and 3 to either CAMM lacking sucrose (no sucrose), CAMM with 0.2% sucrose (constant sucrose), or were transferred twice a day for 1 hr each time into 0.2% sucrose (intermittent sucrose). Four types of analysis were performed: 1) fluorescence microscopy of biofilms stained with Syto 9 and hexidium idodine to determine the biofilm architecture, 2) quantitative PCR (qPCR) to determine the cell number of each species per cm2, 3) vertical scanning interferometry (VSI) to determine the cariogenic potential of the biofilms, and 4) tomographic pH imaging using radiometric fluorescence microscopy after exposure to pH sensitive nanoparticles to measure the micro-environmental pH. The qualitative and quantitative results reveal the expected dynamics of the community profile when exposed to different sucrose conditions and the cariogenic potential of this in vitro four-species anaerobic biofilm model, thus confirming its usefulness for future analysis of primary and secondary dental caries.

Sobre la forma de garantizar la trazabilidad metrológica en los sistemas hospitalarios

Dentro del equipamiento que existen en los hospitales, se realizan muy diversas actuaciones para el correcto funcionamiento de los equipos que se destinan, tanto a medir sobre el paciente o para tratamiento sobre estos mismos, el abanico de equipos que existen es muy grande, sin contar con los que directamente se instalan o, más correctamente, se implantan en el paciente. Los hospitales tienen establecidas pautas de mantenimiento tanto correctivo como preventivo, también de verificación relativa a seguridad y, en algunos casos, acciones que van destinadas a comprobar las magnitudes en los equipos. Esto último no es lo normal, de hecho, en muchos casos, la mayoría, se desconoce cómo poder comprobar que las magnitudes de un equipo se pueden trazar a un patrón de medida nacional. La investigación se ha desarrollado para determinar, siendo algo que esta patente hasta en la normalización ISO, que no existen para muchos equipos principios establecidos para garantizar la trazabilidad metrológica da las magnitudes físicas en las que trabaja durante su vida útil. Debido a la amplitud de este campo, el desarrollo de investigación se ha llevado en un formato piramidal, desde las necesidades para poder llegar a un estudio concreto de equipos concretos (Termómetros Clínicos, Tensiómetros, Ultrasonidos y Onda Corta). Para llegar a estos se ha realizado un estudio de cómo se encuentra la trazabilidad metrológica en todo el espectro, desde la gestión hasta llegar a casos particulares. Estudiando la gran cantidad de documentación existente y llegando a determinar el campo de trabajo en la metrología hospitalaria, clasificando los equipos y determinando como se encuentran definidos los métodos que llevan a realidad la trazabilidad metrológica, en la normalización. Un grupo no muy trabajado de los equipos electromédicos son los que se encuentran dentro de los tratamientos de Rehabilitación de traumatología, siendo equipos de uso muy habitual en los centros de salud para las lesiones musculares y óseas. A este asunto se ha dedicado un esfuerzo extra, con un reporte histórico de su origen y en qué punto se encuentran ahora. Se han estudiado dentro de estos equipos los de tratamiento de ultrasonidos, de diatermia y onda corta, que son los más representativos de los equipos destinados a electro terapia, pero hay que tener en cuenta que hay campos que se han mencionado globalmente de la rehabilitación, como son la terapia o la biomecánica, de forma similar a otras tecnologías sanitarias de otras especialidades (radiología o análisis clínico). Hay, también, dentro de la terapia, todos esos tratamientos propios de gimnasio, como son poleas, pesas, mesas de masajes, balones, etc… Que su metrología es sencilla, son equipos que existen medios de comprobación de las magnitudes que utilizan por la existencia en otras actividades de la industria (balanzas, pesas, etc). La investigación realizada ha pretendido evaluar todo el global y llegar a casos concretos para determinar cómo está la situación. Llegando a desarrollar una clasificación de equipos y de normas, que permiten vislumbrar las necesidades metrológicas y permitiría establecer una Gestión de la Trazabilidad Metrológica, concluyendo en el estudio para los equipos que se han mencionado. ABSTRACT Within the equipment that exist in the hospitals, there are very diverse performances for the correct operation of the equipment that is intended both to measure on the patient or for treatment on these same, the range of equipment available is very large, without which directly are installed or, more correctly, are implanted in the patient. The hospitals have established patterns of maintenance both corrective and preventive, also of verification of security and, in some cases, actions which are intended to check the magnitudes in the equipment. This last is not normal, in fact, in many cases, the majority, it is not known as to be able to check that the magnitudes of a computer can be traced to a pattern of national measure. The research has been developed to determine, is something that this patent up to the International Organization for Standardization (ISO), that do not exist for many teams established principles to ensure the metrological traceability gives the physical quantities in which they work during its useful life. Due to the breadth of this field, the development of research has been conducted in a pyramid format, from the needs to be able to reach a specific study of specific equipment (Clinical Thermometers, Tensiometers, Ultrasound, and Short Wave). To reach theme, has been carried out a study of how is the metrological traceability across the entire spectrum, from the management to individuals cases. Considering the large amount of existing documentation and arriving to determine the labor camp in the metrology hospital, sorting equipment and determining how are defined the methods that lead to reality the metrological traceability, in the standardization. A group not too worked for medical electrical equipment are found within the rehabilitation treatment of traumatology, being equipment of very common use in the health centers for muscle injuries and bone. In this matter has been dedicated an extra effort, with a report history of its origin and where they are now. We have studied within these teams about the treatment of ultrasound, diathermy, short wave, which are the most representative of the equipment that is destined to electro therapy, but it must be borne in mind that there are fields that have been mentioned globally of rehabilitation, as are the therapy or the biomechanics, similar to other health technologies from other specialties (radiology or clinical analysis). There is, also, within the therapy, all these treatments own gym, such as pulleys, weights, tables of massages, balls, etc… that its metrology is simple, are computers that there are means of verification of the magnitudes that used by the existence in other activities of the industry (scales, weights, etc). The research carried out has tried to assess the entire global and reach specific cases to determine the situation as it is. Arriving in the development of a classification of equipment and standards, which give us a glimpse of the metrological needs and establish a Management of the Metrological Traceability, concluding in the study for computers that have been mentioned.

Estudo morfológico e eletrofisiológico dos efeitos da injeção intravítrea de ácido micofenólico em coelhos utilizando um modelo de uveíte crônica experimental

Uveítes são inflamações intra-oculares geralmente crônicas e constituem uma das principais causas de cegueira no mundo. Os corticosteroides são a droga de primeira escolha para o tratamento das uveítes não infecciosas, mas muitas vezes há necessidade do uso de outras drogas imunossupressoras. O micofenolato de mofetila (MMF) é um potente imunossupressor administrado por via oral que vem sendo utilizado com sucesso no tratamento das uveítes, mas cujos efeitos colaterais muitas vezes tornam necessária sua suspensão. O MMF é uma pró-droga, que é transformada no fígado em ácido micofenólico (MPA), o imunossupressor ativo. Para minimizar os efeitos colaterais do uso do MPA e permitir que o olho receba uma dose maior da droga, testamos os efeitos da injeção intravítrea do MPA em um modelo de uveíte crônica experimental (UCE) em olhos de coelhos. Os objetivos deste estudo foram: 1) reproduzir um modelo de UCE em coelhos através da injeção intravítrea de M. tuberculosis; 2) estabelecer uma dose segura de MPA a ser injetada no vítreo; e 3) analisar os efeitos morfológicos, clínicos e eletrofisiológicos da injeção intravítrea de MPA em coelhos utilizados como modelo de UCE. O modelo de UCE reproduzido apresentou uma inflamação autolimitada, possuindo um pico de inflamação no 17° dia após a indução da uveíte. As doses de MPA testadas (0,1 e 1mg) não foram toxicas para a retina do coelho. O modelo de UCE recebeu uma injeção intravítrea de 0,1mg de MPA e as análises clinicas demonstraram uma redução na inflamação. As análises realizadas com o eletrorretinograma (ERG) também apontaram uma melhora na inflamação através da recuperação da latência das ondas-a e b (fotópicas e escotópica) e recuperação da amplitude da onda-a (fotópica). As análises morfológicas com HE não apresentaram alterações na estrutura retinia, porem a imunohistoquimica para proteína GFAP evidenciou gliose das células de Müller, sinalizando um processo inflamatório. Concluímos que o modelo de UCE reproduziu uma uveíte anterior semelhante à uveíte causada em humanos e a dose de MPA utilizada apresentou efeitos terapêuticos durante o pico de inflamação, mostrando uma diminuição da inflamação e promovendo a recuperação de fotorreceptores e células bipolares-ON. Este resultado faz das injeções intravítreas de MPA um recurso promissor no tratamento de uveítes. Porém, novos experimentos são necessários para padronizar os resultados encontrados

Novel glucose sensor based on enzymeimmobilized 81° tilted fiber grating

We demonstrate a novel glucose sensor based on an optical fiber grating with an excessively tilted index fringe structure and its surface modified by glucose oxidase (GOD). The aminopropyltriethoxysilane (APTES) was utilized as binding site for the subsequent GOD immobilization. Confocal microscopy and fluorescence microscope were used to provide the assessment of the effectiveness in modifying the fiber surface. The resonance wavelength of the sensor exhibited red-shift after the binding of the APTES and GOD to the fiber surface and also in the glucose detection process. The red-shift of the resonance wavelength showed a good linear response to the glucose concentration with a sensitivity of 0.298nm(mg/ml)-1 in the very low concentration range of 0.0∼3.0mg/ml. Compared to the previously reported glucose sensor based on the GOD-immobilized long period grating (LPG), the 81° tilted fiber grating (81°-TFG) based sensor has shown a lower thermal cross-talk effect, better linearity and higher Q-factor in sensing response. In addition, its sensitivity for glucose concentration can be further improved by increasing the grating length and/or choosing a higher-order cladding mode for detection. Potentially, the proposed techniques based on 81°-TFG can be developed as sensitive, label free and micro-structural sensors for applications in food safety, disease diagnosis, clinical analysis and environmental monitoring.

Motion correction algorithm of lung tumors for respiratory gated PET images

Respiratory gating in lung PET imaging to compensate for respiratory motion artifacts is a current research issue with broad potential impact on quantitation, diagnosis and clinical management of lung tumors. However, PET images collected at discrete bins can be significantly affected by noise as there are lower activity counts in each gated bin unless the total PET acquisition time is prolonged, so that gating methods should be combined with imaging-based motion correction and registration methods. The aim of this study was to develop and validate a fast and practical solution to the problem of respiratory motion for the detection and accurate quantitation of lung tumors in PET images. This included: (1) developing a computer-assisted algorithm for PET/CT images that automatically segments lung regions in CT images, identifies and localizes lung tumors of PET images; (2) developing and comparing different registration algorithms which processes all the information within the entire respiratory cycle and integrate all the tumor in different gated bins into a single reference bin. Four registration/integration algorithms: Centroid Based, Intensity Based, Rigid Body and Optical Flow registration were compared as well as two registration schemes: Direct Scheme and Successive Scheme. Validation was demonstrated by conducting experiments with the computerized 4D NCAT phantom and with a dynamic lung-chest phantom imaged using a GE PET/CT System. Iterations were conducted on different size simulated tumors and different noise levels. Static tumors without respiratory motion were used as gold standard; quantitative results were compared with respect to tumor activity concentration, cross-correlation coefficient, relative noise level and computation time. Comparing the results of the tumors before and after correction, the tumor activity values and tumor volumes were closer to the static tumors (gold standard). Higher correlation values and lower noise were also achieved after applying the correction algorithms. With this method the compromise between short PET scan time and reduced image noise can be achieved, while quantification and clinical analysis become fast and precise.

Motion Correction Algorithm of Lung Tumors for Respiratory Gated PET Images

Respiratory gating in lung PET imaging to compensate for respiratory motion artifacts is a current research issue with broad potential impact on quantitation, diagnosis and clinical management of lung tumors. However, PET images collected at discrete bins can be significantly affected by noise as there are lower activity counts in each gated bin unless the total PET acquisition time is prolonged, so that gating methods should be combined with imaging-based motion correction and registration methods. The aim of this study was to develop and validate a fast and practical solution to the problem of respiratory motion for the detection and accurate quantitation of lung tumors in PET images. This included: (1) developing a computer-assisted algorithm for PET/CT images that automatically segments lung regions in CT images, identifies and localizes lung tumors of PET images; (2) developing and comparing different registration algorithms which processes all the information within the entire respiratory cycle and integrate all the tumor in different gated bins into a single reference bin. Four registration/integration algorithms: Centroid Based, Intensity Based, Rigid Body and Optical Flow registration were compared as well as two registration schemes: Direct Scheme and Successive Scheme. Validation was demonstrated by conducting experiments with the computerized 4D NCAT phantom and with a dynamic lung-chest phantom imaged using a GE PET/CT System. Iterations were conducted on different size simulated tumors and different noise levels. Static tumors without respiratory motion were used as gold standard; quantitative results were compared with respect to tumor activity concentration, cross-correlation coefficient, relative noise level and computation time. Comparing the results of the tumors before and after correction, the tumor activity values and tumor volumes were closer to the static tumors (gold standard). Higher correlation values and lower noise were also achieved after applying the correction algorithms. With this method the compromise between short PET scan time and reduced image noise can be achieved, while quantification and clinical analysis become fast and precise.

Construção de um Referencial de Competências dos Técnicos de Análises Clínicas e Saúde Pública

O presente estudo teve como objectivo construir e validar um referencial de competências dos Técnicos de Análises Clínicas e Saúde Pública. Para a concretização deste objectivo construi-se uma entrevista estruturada com 15 questões de resposta aberta aplicada a uma amostra de 10 Técnicos de Análises Clínicas e Saúde Pública. Os dados foram recolhidos, culminaram num questionário de resposta fechada constituído por 36 competências e aplicado a 100 técnicos. Dos resultados do tratamento deste, derivou um outro questionário com 26 competências, relativamente às quais, além da classificação das mesmas, era solicitado emissão de opinião técnica. Este instrumento foi aplicado a 10 peritos (professores que ministraram ou ministram na Licenciatura de Análises Clínicas e Saúde Pública e/ou investigadores desta área de estudos). Daí resultaram 22 competências que foram sujeitas a validação semântica e que constituíram o referencial de competências. O conjunto que competências a que se chegou foi trabalhado em clusters de competências pessoais, interpessoais e instrumentais e para se verificar a sua fiabilidade interna aplicou-se o Alfa de Cronbach. O referencial de competências constituído foi validado através de um Focus Group de técnicos. A construção do referencial de competências dos Técnicos de Análises Clínicas e Saúde Pública permitiu dar a conhecer as competências essenciais à apresentação de um bom desempenho profissional, quando sujeitos a avaliação por competências, e o desenvolvimento de estudos acerca desta profissão. / The following paper aimed to develop and validate a reference of the Clinical Analysis and Public Health technicians’ competences. In order to achieve this purpose it was put together a structured interview based on fifteen open-response questions. This interview was applied to a sample of ten Clinical Analysis and Public Health technicians. The collected data of this interview led to a closed response questionnaire including thirty-six competences, which was then administered to a hundred technicians. Subsequently, the data collected in this questionnaire let to another different questionnaire composed of twenty-six competences. This time, besides their classification it was required to issue a technical opinion. This research instrument was then administered to ten experts (teachers who minister or ministered the Degree of Clinical Analysis and Public Health and / or researchers in this field of study). This last resulted into twenty-two competences which were subjected to semantic validation and afterwards composed the reference of competences. The reached reference of competences was then worked on to clusters of personal, interpersonal and instrumental competences whose liability was checked through the Cronbach's Alpha. The reference of competences was then validated through a technician’s Focus Group. The building of this Clinical Analysis and Public Health technicians’ reference of competences was important to acknowledge the important competences necessary to perform this Job well and professionally.

Sonolência Diurna Excessiva e Otimismo em Estudantes do 1º Ano do Ensino Superior

Os principais objetivos do presente estudo são analisar a prevalência da sonolência diurna excessiva (SDE) em estudantes do Ensino Superior (ES), bem como os seus níveis de otimismo. Participaram no presente estudo 162 estudantes universitários de várias faculdades de ES privado, de ambos os sexos, com idades compreendidas entre os 17 e os 46 anos (M=20,49; DP=5,153) e que frequentavam diferentes cursos (Psicologia, Ciências da Comunicação, Análises Clínicas, Ciências Farmacêuticas e Fisioterapia). O protocolo de recolha de dados foi constituído por um questionário sociodemográfico, dois itens do Questionário do Estado de Saúde (SF-36), a Escala de Sonolência de Epworth (ESE) e a Escala de Orientação para a Vida (LOT-R). A ESE apresentou um valor de alfa de Cronbach de α=0,68 e o LOT-R um valor de alfa de Cronbach de α=0,84. Os principais resultados descritivos mostram uma pontuação média na ESE de 8,98 (DP=3,85), que corresponde a uma prevalência de 32,1% (n=52) de SDE. A pontuação média obtida com a LOT-R foi de 14,3 (DP=4,77), que corresponde a 65,4% (n=106) de estudantes otimistas. Foram encontradas correlações estatisticamente significativas entre a idade e a SDE, mas não entre a SDE e as variáveis de perceção de saúde. Os resultados diferenciais não apresentaram significância estatística na SDE, quando o sexo, o curso e a situação de residência foram tidos em consideração. A idade foi assumida, nas análises de regressão linear, como variável preditora explicativa de 3% da variância dos resultados da ESE. Relativamente ao otimismo, este mostrou-se correlacionado com a perceção do estado de saúde no momento, mas não com a SDE, idade e transição de saúde. Não foi encontrada significância estatística no otimismo quando o sexo, o curso e a situação de residência foram considerados. As análises de regressão linear identificaram como modelo preditor explicativo de cerca de 12% da variação total do LOT-R o modelo que integra a idade e a perceção do estado de saúde no momento. Estudos posteriores devem ser realizados para se aferir a prevalência de SDE e caraterizar o otimismo em estudantes universitários, bem como a relação entre ambos e com outras variáveis relevantes, de foma a apoiar, adequadamente a intervenção.