Explicação e argumentação na linguagem da criança: diferenças e intersecções em dois estudos de caso

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Las ascensión de los Iriarte. A propósito de la relación entre políticos y literatos en la España del absolutismo borbónico

[ES]Al amparo del tío paterno, que llamó a los sobrinos a la corte y supo servirse de sus influencias para situarlos convenientemente, los Iriarte constituyeron un importante núcleo intelectual y político en la España del siglo XVIII. A pesar de su singularidad, la formidable ascensión del clan familiar no ha sido suficientemente explicada, a pesar de que en ella se concitan de manera ejemplar las particulares circunstancias que orientaron las relaciones entre políticos y literatos en la España del absolutismo borbónico.

Research on the vocal culture of Orcinus orca in the Loro Parque, Tenerife: a pilot study

This research is focussed on the study of Orcinus orca's communication system. The analysis of vocalizations emitted by marine mammals has started in the '80s and most studies have been carried out in the wild. In this regard the most studied animal has been common dolphin (Tursiops truncatus) as the numerous presence of captive individuals worldwide made researches easier to be carried out. Studies about Orcinus orca's vocalizations have mainly been carried out in the wild (most in British Columbia) because its maintenance in a controlled environment results to be very difficult, only 17 among parks and oceanaria worldwide have some Orcinus orca (45 overall among which 64% born in captivity). These researches showed that Orcinus orca emit three main different types of sounds, classified as: whistles, clicks and calls. Besides, it was discovered that different groups (pods) produce sounds belonging only to the relevant pod (dialects). It is rare to find two pods sharing some calls. The two pods usually live in adjacent areas and can form a clan. This study was carried out in a controlled environment in the Orca ocean structure (Loro Parque, Tenerife, Spain) where, at the moment (March 2012) 6 individuals are hosted. Here it was developed an automatic sound recording system. Thanks to the use of suitable mathematical algorithms that allow to isolate only "interesting" sound events that differ from the "background noise", it was possible to create a database. The visualization of the sound events collected in the database is carried out with the use of a software. By looking at this output and at the observation register we could match the animal to the sound produced. Three situations were detected and studied: 1) Chosen alone: the animal chooses to go to the recording pool but it is free to move to another pool with other individuals. 2) Put alone: the animal is put alone in the recording pool. 3) With other orcas: more animals are together in the recording pool. The statistic analysis show that animals emit more vocalizations when they are in the situation "Chosen alone". The research will continue in order to observe eventual differences in the individual repertoire of each Orcinus orca.

Governing individual learning in the transition phase of software maintenance offshoring: a dynamic perspective

Prior studies suggest that clients need to actively govern knowledge transfer to vendor staff in offshore outsourcing. In this paper, we analyze longitudinal data from four software maintenance offshore out-sourcing projects to explore why governance may be needed for knowledge transfer and how governance and the individual learning of vendor engineers inter-act over time. Our results suggest that self-control is central to learning, but may be hampered by low levels of trust and expertise at the outset of projects. For these foundations to develop, clients initially need to exert high amounts of formal and clan controls to enforce learning activities against barriers to knowledge sharing. Once learning activities occur, trust and expertise increase and control portfolios may show greater emphases on self-control.

Managing Knowledge Transfer in Software-Maintenance Outsourcing Transitions: A System-Dynamics Perspective

The existing literature suggests that transitions in software-maintenance offshore outsourcing projects are prone to knowledge transfer blockades, i.e. situations in which the activities that would yield effective knowledge transfer do not occur, and that client management involvement is central to overcome them. However, the theoretical understanding of the knowledge transfer blockade is limited, and the reactive management behavior reported in case studies suggests that practitioners may frequently be astonished by the dynamics that may give rise to the blockade. Drawing on recent research from offshore sourcing and reference theories, this study proposes a system dynamics framework that may explain why knowledge transfer blockades emerge and how and why client management can overcome the blockade. The results suggest that blockades may emerge from a vicious circle of weak learning due to cognitive overload of vendor staff and resulting negative ability attributions that result in reduced helping behavior and thus aggravate cognitive load. Client management may avoid these vicious circles by selecting vendor staff with strong prior related experience. Longer phases of coexistence of vendor staff and subject matter experts and high formal and clan controls may also mitigate vicious circles.

Function of C1A barley peptidases and their inhibitors (cystatins) in barley seed germination : Funcion de las peptidasas C1A de cebada y sus inhibidores (cistatinas) en la germinacion de la semilla

Plant proteolysis is a metabolic process where specific enzymes called peptidases degrade proteins. In plants, this complex process involves broad metabolic networks and different sub-cellular compartments. Several types of peptidases take part in the proteolytic process, mainly cysteine-, serine-, aspartyl- and metallo- peptidases. Among the cysteine-peptidases, the papain-like or C1A peptidases (family C1, clan CA) are extensively present in land plants and are classified into catepsins L-, B-, H- and Flike. The catalytic mechanism of these C1A peptidases is highly conserved and involves the three amino acids Cys, His and Asn in the catalytic triad, and a Gln residue which seems essential for maintaining an active enzyme conformation. These proteins are synthesized as inactive precursors, which comprise an N-terminal signal peptide, a propeptide, and the mature protein. In barley, we have identified 33 cysteine-peptidases from the papain-like family, classifying them into 8 different groups. Five of them corresponded to cathepsins L-like (5 subgroups), 1 cathepsin B-like group, 1 cathepsin F-like group and 1 cathepsin H-like group. Besides, C1A peptidases are the specific targets of the plant proteinaceous inhibitors known as phytocystatins (PhyCys). The cystatin inhibitory mechanism is produced by a tight and reversible interaction with their target enzymes. In barley, the cystatin gene family is comprised by 13 members. In this work we have tried to elucidate the role of the C1A cysteine-peptidases and their specific inhibitors (cystatins) in the germination process of the barley grain. Therefore, we selected a representative member of each group/subgroup of C1A peptidases (1 cathepsin B-like, 1 cathepsin F-like, 1 cathepsin H-like and 5 cathepsins L-like). The molecular characterization of the cysteine-peptidases was done and the peptidase-inhibitor interaction was analyzed in vitro and in vivo. A study in the structural basis for specificity of pro-peptide/enzyme interaction in barley C1A cysteine-peptidases has been also carried out by inhibitory assays and the modeling of the three-dimensional structures. The barley grain maturation produces the accumulation of storage proteins (prolamins) in the endosperm which are mobilized during germination to supply the required nutrients until the photosynthesis is fully established. In this work, we have demonstrated the participation of the cysteine-peptidases and their inhibitors in the degradation of the different storage protein fractions (hordeins, albumins and globulins) present in the barley grain. Besides, transgenic barley plants overexpressing or silencing cysteine-peptidases or cystatins were obtained by Agrobacterium-mediated transformation of barley immature embryos to analyze their physiological function in vivo. Preliminary assays were carried out with the T1 grains of several transgenic lines. Comparing the knock-out and the overexpressing lines with the WT, alterations in the germination process were detected and were correlated with their grain hordein content. These data will be validated with the homozygous grains that are being produced through the double haploid technique by microspore culture. Resumen La proteólisis es un proceso metabólico por el cual se lleva a cabo la degradación de las proteínas de un organismo a través de enzimas específicas llamadas proteasas. En plantas, este complejo proceso comprende un entramado de rutas metabólicas que implican, además, diferentes compartimentos subcelulares. En la proteólisis participan numerosas proteasas, principalmente cisteín-, serín-, aspartil-, y metalo-proteasas. Dentro de las cisteín-proteasas, las proteasas tipo papaína o C1A (familia C1, clan CA) están extensamente representadas en plantas terrestres, y se clasifican en catepsinas tipo L, B, H y F. El mecanismo catalítico de estas proteasas está altamente conservado y la triada catalítica formada por los aminoácidos Cys, His y Asn, y a un aminoácido Gln, que parece esencial para el mantenimiento de la conformación activa de la proteína. Las proteasas C1A se sintetizan como precursores inactivos y comprenden un péptido señal en el extremo N-terminal, un pro-péptido y la proteína madura. En cebada hemos identificado 33 cisteín-proteasas de tipo papaína y las hemos clasificado filogenéticamente en 8 grupos diferentes. Cinco de ellos pertenecen a las catepsinas tipo L (5 subgrupos), un grupo a las catepsinas tipo-B, otro a las catepsinas tipo-F y un último a las catepsinas tipo-H. Las proteasas C1A son además las dianas específicas de los inhibidores protéicos de plantas denominados fitocistatinas. El mecanismo de inhibición de las cistatinas está basado en una fuerte interacción reversible. En cebada, se conoce la familia génica completa de las cistatinas, que está formada por 13 miembros. En el presente trabajo se ha investigado el papel de las cisteín-proteasas de cebada y sus inhibidores específicos en el proceso de la germinación de la semilla. Para ello, se seleccionó una proteasa representante de cada grupo/subgrupo (1 catepsina tipo- B, 1 tipo-F, 1 tipo-H, y 5 tipo-L, una por cada subgrupo). Se ha llevado a cabo su caracterización molecular y se ha analizado la interacción enzima-inhibidor tanto in vivo como in vitro. También se han realizado estudios sobre las bases estructurales que demuestran la especificidad en la interacción enzima/propéptido en las proteasas C1A de cebada, mediante ensayos de inhibición y la predicción de modelos estructurales de la interacción. Finalmente, y dado que durante la maduración de la semilla se almacenan proteínas de reserva (prolaminas) en el endospermo que son movilizadas durante la germinación para suministrar los nutrientes necesarios hasta que la nueva planta pueda realizar la fotosíntesis, en este trabajo se ha demostrado la participación de las cisteínproteasas y sus inhibidores en la degradación de las diferentes tipos de proteínas de reserva (hordeinas, albúmins y globulinas) presentes en el grano de cebada. Además, se han obtenido plantas transgénicas de cebada que sobre-expresan o silencian cistatinas y cisteín-proteasas con el fin de analizar la función fisiológica in vivo. Se han realizado análisis preliminares en las semillas T1 de varias líneas tránsgenicas de cebada y al comparar las líneas knock-out y las líneas de sobre-expresión con las silvestres, se han detectado alteraciones en la germinación que están además correlacionadas con el contenido de hordeinas de las semillas. Estos datos serán validados en las semillas homocigotas que se están generando mediante la técnica de dobles haploides a partir del cultivo de microesporas.

Cysteine-proteases and cystatins from barley: molecular and functional characterization in housekeeping and defense processes

Plant cysteine-proteases (CysProt) represent a well-characterized type of proteolytic enzymes that fulfill tightly regulated physiological functions (senescence and seed germination among others) and defense roles. This article is focused on the group of papain-proteases C1A (family C1, clan CA) and their inhibitors, phytocystatins (PhyCys). In particular, the protease–inhibitor interaction and their mutual participation in specific pathways throughout the plant's life are reviewed. C1A CysProt and PhyCys have been molecularly characterized, and comparative sequence analyses have identified consensus functional motifs. A correlation can be established between the number of identified CysProt and PhyCys in angiosperms. Thus, evolutionary forces may have determined a control role of cystatins on both endogenous and pest-exogenous proteases in these species. Tagging the proteases and inhibitors with fluorescence proteins revealed common patterns of subcellular localization in the endoplasmic reticulum–Golgi network in transiently transformed onion epidermal cells. Further in vivo interactions were demonstrated by bimolecular fluorescent complementation, suggesting their participation in the same physiological processes.

A VIOLÊNCIA CONTRA AS MULHERES NA VIDA COTIDIANA Um estudo do Livro da Aliança a partir de Êxodo 20,22-23,19

O espaço social subjacente à unidade literária de Êxodo 20,22-23,19 pressupõe uma sociedade agrária. Aparentemente é monótona, marcada por inúmeros conflitos sociais. O contexto social é de empobrecimento das famílias clânico-tribais israelitas. A nova economia se organiza em torno do santuário. Na pesquisa, há bastante consenso quanto à origem desta unidade literária conhecida como Livro da Aliança. É literatura jurídica de caráter religioso. Uma prescrição jurídica não precede as condições da realidade a que vai se referir, mas prescreve sobre as condições e situações já existentes. Na pesquisa clássica atual, encontram-se duas grandes correntes sobre a origem e a época desta literatura. Uma defende que o Livro da Aliança remonta à época pré-estatal, passagem do tribalismo para a monarquia; a outra argumenta que o Livro da Aliança, enquanto corpus codificado de leis, é um produto tardio , possivelmente surgido no final do século VIII ou início do século VII a.C. O Livro da Aliança é a base literária da presente pesquisa. Quanto à origem e à época do Livro da Aliança, sigo a corrente que defende ser o texto da época final do período tribal, anterior à monarquia. Esta época foi marcada por grandes mudanças econômicas: passagem de uma economia solidária de subsistência para uma economia de concentração do produto. A tese consiste em analisar a violência contra as mulheres, estruturada no discurso jurídico do Livro da Aliança. Busca-se desvendar os mecanismos que justificam e naturalizam as práticas de violência. O destaque é a violência contra as mulheres escravas, contra as filhas e, de modo especial, enfatizo as violências contra as mulheres feiticeiras. Evidencio três categorias de escravas prescritas no texto: as escravas domésticas, que sofrem violências físicas, podendo chegar até à morte debaixo do castigo da vara; as escravas temporárias, que têm seus olhos destruídos e os seus dentes quebrados; e as filhas que são vendidas como escravas. Sua sexualidade é transformada em mercadoria. Há filhas que são seduzidas, violadas e submetidas como mulher ao seu estuprador. O único grupo social descrito a partir da sua função pública são as feiticeiras. As violências são institucionais e sexistas. O patriarcado é o princípio organizador da sociedade. A característica do Livro da Aliança é marcadamente androcêntrica.(AU)

A VIOLÊNCIA CONTRA AS MULHERES NA VIDA COTIDIANA Um estudo do Livro da Aliança a partir de Êxodo 20,22-23,19

O espaço social subjacente à unidade literária de Êxodo 20,22-23,19 pressupõe uma sociedade agrária. Aparentemente é monótona, marcada por inúmeros conflitos sociais. O contexto social é de empobrecimento das famílias clânico-tribais israelitas. A nova economia se organiza em torno do santuário. Na pesquisa, há bastante consenso quanto à origem desta unidade literária conhecida como Livro da Aliança. É literatura jurídica de caráter religioso. Uma prescrição jurídica não precede as condições da realidade a que vai se referir, mas prescreve sobre as condições e situações já existentes. Na pesquisa clássica atual, encontram-se duas grandes correntes sobre a origem e a época desta literatura. Uma defende que o Livro da Aliança remonta à época pré-estatal, passagem do tribalismo para a monarquia; a outra argumenta que o Livro da Aliança, enquanto corpus codificado de leis, é um produto tardio , possivelmente surgido no final do século VIII ou início do século VII a.C. O Livro da Aliança é a base literária da presente pesquisa. Quanto à origem e à época do Livro da Aliança, sigo a corrente que defende ser o texto da época final do período tribal, anterior à monarquia. Esta época foi marcada por grandes mudanças econômicas: passagem de uma economia solidária de subsistência para uma economia de concentração do produto. A tese consiste em analisar a violência contra as mulheres, estruturada no discurso jurídico do Livro da Aliança. Busca-se desvendar os mecanismos que justificam e naturalizam as práticas de violência. O destaque é a violência contra as mulheres escravas, contra as filhas e, de modo especial, enfatizo as violências contra as mulheres feiticeiras. Evidencio três categorias de escravas prescritas no texto: as escravas domésticas, que sofrem violências físicas, podendo chegar até à morte debaixo do castigo da vara; as escravas temporárias, que têm seus olhos destruídos e os seus dentes quebrados; e as filhas que são vendidas como escravas. Sua sexualidade é transformada em mercadoria. Há filhas que são seduzidas, violadas e submetidas como mulher ao seu estuprador. O único grupo social descrito a partir da sua função pública são as feiticeiras. As violências são institucionais e sexistas. O patriarcado é o princípio organizador da sociedade. A característica do Livro da Aliança é marcadamente androcêntrica.(AU)

2002 Parliamentary elections in Ukraine: Events, results and consequences; Ukraine's Parliamentary system after the elections. OSW Study 8/2003

2002 elections: On 31 March 2002, parliamentary elections were held in Ukraine. As expected, they were a major success for the centrist-rightist coalition focused around former Prime Minister Viktor Yuschenko. The communists emerged significantly weaker from the vote, and the "party of power" achieved a poor result. Yet, due to the mixed electoral law (half of the deputies were elected in single-mandate districts), the latter block, firmly supported by President Leonid Kuchma, resulted as the main force in Parliament. The results of particular parties and blocks were as follows: Viktor Yuschenko's Block received 23.57% of votes and 112 seats, the Communist Party of Ukraine - 19.98% of votes and 66 seats, the "For One Ukraine" block - 11.77% of votes and 101 seats, Yulia Tymoshenko's Block - 7.26% of votes and 22 seats, the Socialist Party of Ukraine - 6.87% of votes and 22 seats, and the Social Democratic Party of Ukraine (united) - 6.27% of votes and 24 seats. This shows how the mixed electoral regulations favour "For One Ukraine" and act against Yuschenko's block. One should note, however, that the latter gained the support of less than one quarter of voters. After the election: The dominant force in Ukraine's Verkhovna Rada, elected in March 2002, are the deputies of "One Ukraine", a fraction of the pro-presidential centre. "One Ukraine" has refused to admit any of the opposition's representatives (either from the right or left wings) into the parliament's presidium, but has accepted opposition-appointed heads of many parliamentary commissions. Viktor Yuschenko's "Our Ukraine", which has been the largest parliamentary fraction since June, attempted to proclaim itself the centre of the parliamentary majority, but its policy was awkward and inconsistent, and the main success of this club was that it didn't break up. Viktor Yuschenko's moves have been particularly incoherent and they undermined the image of Yuschenko as Ukraine's future leader, created throughout the course of the electoral campaign. In autumn, the main oligarchic groups and their representative fractions ("One Ukraine", which proved to be a useless instrument, was dissolved in June), reached a compromise with the president. It was agreed that the new prime minister should be a Donetsk clan representative (Viktor Yanukovych), and that the Dnipropetrovsk clan should appoint the president of the National Bank of Ukraine (this position went to Serhij Tihipko). The Kyiv clan obtained the President's Administration (Viktor Medvedchuk was appointed in spring) and a considerable number of parliamentary commissions. The president's interests in the government are to be protected by Mykola Azarov, former Head of the State Tax Administration. This compromise "package" was designed to secure the shares of the main oligarchic clans in the power and the president's strong position as mediator.

The Limited Influence of the European Union in Armenia and Azerbaijan: A Domestic Explanation. EU Diplomacy Paper 9/2015

This paper seeks to explain why the European Union (EU) has had limited influence in Armenia and Azerbaijan in the framework of the European Neighbourhood Policy (ENP). Combining approaches from external governance, norm diffusion and structural foreign policy, it offers an explanation based on domestic factors in the two countries: the political regime, state capacity, political structures, domestic incentives and the perceived legitimacy of EU rules. Although willingness to reform appears to exist in Armenia, such willingness remains constrained by the country’s vulnerable geopolitical location and high dependence on Russia. By contrast, none of the domestic preconditions for EU influence identified by the analytical framework were found in Azerbaijan. The author argues that the Eastern Partnership has not properly addressed the extent to which the clan structures feed into informal political practices and enforce the sustainability of an existing regime in both countries, and that, in addition, the EU has underestimated the multipolar environment which the two countries have to operate in, making it unlikely that the current policy can reach its objectives in Armenia and Azerbaijan.

The yellow bittern, and other plays.

Title on spine: Three plays.

Waifs and strays of Celtic tradition... Argyllshire series.

"Initiated and directed by Lord Archibald Campbell."

Wounds in the rain: war stories,

The price of the harness.--The lone charge of William B. Perkins.--The clan of No-name.--God rest ye, merry gentlemen.--The revenge of the Adolphus.--The sergeant's private madhouse.--Virtue in war.--Marines signalling under fire at Guantanamo.--This majestic lie.--War memories.--The second generation.

A minister of grace,

Of the clan of God.--As one having authority.--Wild woodland.--A moment of revolt.--"Noblesse oblige."--That other Eileen.--Adjustment.--The little queens of death.--Powers of darkness.