Dietary energy requirement of piracanjuba fingerlings, Brycon orbignyanus, and relative utilization of dietary carbohydrate and lipid

Ten isonitrogenous casein-gelatin-based diets were formulated to contain five estimated metabolizable energy concentrations (10.92, 12.29, 13.63, 14.82 and 16.16 kJ g -1) at two carbohydrate-to-lipid ratios (CHO : L, 5.3 and 12.8, g : g) in a 5 × 2 factorial arrangement. Each diet was assigned to triplicate groups of 11 piracanjuba fingerlings (5.25 ± 0.14 g) and fed to apparent satiation twice a day for 90 days. Higher daily weight gain was obtained by fish fed the 13.63 kJ g -1 diets for both CHO : L ratios. There was a significant reduction of feed consumption when dietary energy concentration increased above 13.63 kJ g -1. Feed conversion ratio and apparent net energy retention improved as dietary energy increased. Apparent net protein retention tended to be lower in the highest and lowest dietary energy concentrations. The results suggest that dietary lipid energy was more efficiently utilized by piracanjuba fingerlings than carbohydrate energy. Body composition and hepatosomatic index (HSI) were not influenced by dietary CHO : L ratio. However, an increase in dietary energy concentration beyond 13.63 kJ g -1 resulted in a significant increment in lipid deposition, while body moisture and HSI decreased. Our findings indicate that at 300 g kg -1 dietary crude protein, a CHO : L ratio of 5.3 is recommended for piracanjuba, and the required energy is either 13.63 kJ g -1 if raised for aquaculture or 14.82 kJ g -1 if destined to stock enhancement. © 2006 Blackwell Publishing Ltd.

Morphological, cytochemical, and ultrastructural study of thrombocytes and leukocytes in neotropical fish, Brycon orbignyanus Valenciennes, 1850 (Characidae, Bryconinae)

Morphological, cytochemical and ultrastructural studies are important to demonstrate the function of the blood cells, which is very little understood in teleosts. In peripheral blood of piracanjuba' Brycon orbignyanus, thrombocytes, lymphocytes, monocytes, neutrophils and heterophils were studied and characterized. Thrombocytes had a fusiform or oval shape with PAS-positive granules. Lymphocytes presented small size with sparse basophilic cytoplasm. Monocytes were large in size, presented basophilic cytoplasm that may be foamy or vacuolated, with non-specific esterase staining. The neutrophils presented lightly neutrophilic granule cytoplasm, with positivity for PAS and peroxidase. The heterophils were large in size, with eosinophilic and basophilic granules cytoplasm and PAS-positive. Transmission electron microscopy study demonstrated that the thrombocytes, lymphocytes and monocytes features were similar to other teleosts. In ultrastructural study only one type of neutrophils was observed. Cytochemical findings indicated that neutrophils and monocytes of B. orbignyanus may be involved in phagocytosis, and neutrophils play an important microbicidal role.

Mitigating stress effects during transportation of matrinxã(Brycon amazonicus Günther, 1869; Characidae) through the application of calcium sulfate

This study verified the effects of CaSO4 on physiological responses of the tropical fish matrinxãBrycon amazonicus(200.2 ± 51.1 g) in water containing CaSO4 after a 4-h transportation at concentrations of: 0, 75, 150, and 300 mg L-1. Blood samples were collected prior to transportation (initial levels), immediately after packaging, at arrival, and 24 h and 96 h after transportation (recovery). Cortisol levels increased after ackaging (118.2 ± 14.2 ng ml-1), and decreased slightly after transportation in water containing CaSO4 (106.8 ± 14.1), but remained higher than initial levels (21.0 ± 2.6 ng ml)1). Fish kept at 150 mg L-1 CaSO4 reached the pre-transportation levels at 24 h of recovery. Blood glucose increased after transportation in all treatments (8.2 ± 0.2 mmol L-1) and declined after full recovery to values below initial levels (4.8 ± 0.1 mmol L-1). Chloride levels did not change in CaSO4 treatments; serum sodium concentrations decreased after packaging and after transportation. Serum calcium levels did not differ among treatments, but decreased after packaging and increased at 96 h of recovery. Hematocrit and the number of red blood cells were higher in all treatments after packaging and arrival, except in fish exposed to 300 mg L-1 CaSO4. Mean corpuscular volume increased in 75 mg L-1 CaSO4, which reached the higher VCM after transportation. Hemoglobin levels increased only after transportation, regardless of calcium sulfate levels. Handling before transportation and transportation itself were both stressful to fish; calcium sulfate at concentrations tested in the present work had a moderate influence in the reduction of stress responses. © 2009 Blackwell Verlag, Berlin.

Improvement of Matrinxã, Brycon amazonicus, Larviculture by Exposing Eggs to Triiodothyronine

This work evaluated the effect of triiodothyronine (T3) on larviculture of matrinxã, Brycon amazonicus. Oocytes of three females were pooled, fertilized with pooled semen of two males and separated in four batches that were immersed in triiodothyronine solutions as follows: M1 (control - water); M2 (0.01mg/L T3); M3 (0.05mg/L T3); and M4 (0.1mg/L T3). Triiodothyronine did not affect fertilization rate and number of hatched larvae. Weight of hatched larvae was significantly higher in treatments M3 and M4, as well as among larvae sampled at Day 12 in all treatments. After 12d of rearing, biomass gain was higher in the hormone treatments (M1 688±569mg; M2 2436±562mg; M3 3572±569mg; and M4 4129±770mg). In general, coefficients of variation of weight (CVw) and length (CVl) did not differ among treatments and cannibalism was registered between 36 and 72hours post-hatching (h.p.h.) without differences among treatments. Larval survival increased in the hormone treatments (M1 26.5%; M2 37.6%; M3 40.6%; and M4 40.8%). The results indicate that the immersion of matrinxã eggs in triiodothyronine can promote beneficial effects to its larviculture and indicate promising perspectives for culture of this tropical species. © by the World Aquaculture Society 2013.

Efeito do L-triptofano na mediação do estresse de transporte e social do matrinxã (Brycon amazonicus)

Pós-graduação em Aquicultura - FCAV

Desenvolvimento embrionário e larval de Brycon gouldingi (Teleostei, Characidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Elevação plasmática do cortisol aumenta a agressividade em juvenis de matrinxã, Brycon amazonicus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Controle neuroendócrino do comportamento agressivo de juvenis de matrinxã (Brycon amazonicus)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Uso da Aloe vera L. no manejo de matrinxã (Brycon amazonicus)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cortisol na reprodução e no desenvolvimento inicial do matrinxã (Brycon amazonicus)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Isolamento e caracterização de elementos transponíveis em espécies do gênero Brycon (Characidae, Bryconinae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Isolamento e caracterização de marcadores de DNA associados ao sexo e segmentos de DNA repetitivo em espécies do gênero Brycon (Characidae, Bryconinae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Enriquecimento da alimentação das larvas de matrinxã (Brycon amazonicus) com aminoácidos: influência no crescimento inicial e sobrevivência das larvas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização citogenética das espécies matrinxã (Brycon amazonicus), piraputanga (Brycon hilarii) e sua geração filial, utilizados na piscicultura brasileira

Pós-graduação em Ciências Biológicas (Zoologia) - IBB

Viabilidade de embriões de piracanjuba (Brycon orbignyanus) submetidos a diferentes protocolos de resfriamento

Pós-graduação em Aquicultura - FCAV