Seroprevalence estimation and risk factors for A. marginale on smallholder dairy farms in Tanzania

A cross-sectional serological survey of A. marginale was conducted on 200 randomly selected smallholder farms in each of the Tanga and Iringa Regions of Tanzania between January and April 1999. Sera, from dairy cattle of all ages, sexes and breeds were tested for antibodies against A. marginale using an indirect enzyme-linked immunosorbent assay. Antibodies to A. marginale were present in cattle throughout the study areas and the overall prevalence was 20% for Tanga and 37% for Iringa. The forces of infection based on the age seroprevalence profile were estimated at 8 for Tanga and 15 for Iringa per 100 cattle years-risk, respectively. In both regions, seroprevalence increased with age (β = 0.01 and 0.017 per year of age, p < 0.005, in Tanga and Iringa, respectively). Older animals in Iringa were significantly and negatively associated with decreased seropositivity (β = −0.002, p = 0.0029). Further results of logistic regression models reveal that geographic location of animals in Tanga was associated with seropositivity (odds ratio (OR) = 2.94, p = 0.005, for Tanga Rural and OR = 2.38, p = 0.066, for Muheza). Animals acquired as a gift in Iringa had higher odds for seropositivity than brought-in cattle (OR = 2.44, p = 0.005). Our study has identified and quantified some key risk factors that can guide planners devising disease control strategies.

Seroprevalence estimation and risk factors for A-marginale on smallholder dairy farms in Tanzania

A cross-sectional serological survey of A. marginale was conducted on 200 randomly selected smallholder farms in each of the Tanga and Iringa Regions of Tanzania between January and April 1999. Sera, from dairy cattle of all ages, sexes and breeds were tested for antibodies against A. marginale using an indirect enzyme-linked immunosorbent assay. Antibodies to A. marginale were present in cattle throughout the study areas and the overall prevalence was 20% for Tanga and 37% for Iringa. The forces of infection based on the age seroprevalence profile were estimated at 8 for Tanga and 15 for Iringa per 100 cattle years-risk, respectively. In both regions, seroprevalence increased with age (beta = 0.01 and 0.017 per year of age, p < 0.005, in Tanga and Iringa, respectively). Older animals in Iringa were significantly and negatively associated with decreased seropositivity (beta = -0.002, p = 0.0029). Further results of logistic regression models reveal that geographic location of animals in Tanga was associated with seropositivity (odds ratio (OR) = 2.94, p = 0.005, for Tanga Rural and OR = 2.38, p = 0.066, for Muheza). Animals acquired as a gift in Iringa had higher odds for seropositivity than brought-in cattle (OR = 2.44, p = 0.005). Our study has identified and quantified some key risk factors that can guide planners devising disease control strategies.

L’illustration marginale d’un ouvrage profane : étude du manuscrit Paris, Bibliothèque nationale de France, français 95, xiiie siècle (1290)

BnF fr. 95 is a late 13th century manuscript containing Arthurian romances and other fictional and didactic texts. The Estoire del saint Graal and Merlin section is the most highly illuminated, with a rich marginal iconography, an unusual feature in the illustration of lay works and in these texts’ manuscript tradition. This article shows how in Merlin and its Vulgate Sequel marginal scenes overlap with widespread subjects in courtly and chivalric vernacular romances, in contrast with Latin and religious works. The reuse of similar patterns in principal and marginal miniatures, examined in the episode of the Battle of Danablaise, where King Arthur fights the Saxon King Rion, highlights the need for a comprehensive reading of text and images, taking into account the mise en page and the different levels of illustration in the manuscript.

Migratory and Carnivorous Birds in Brazil: Reservoirs for Anaplasma and Ehrlichia Species?

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificação e Prevalência de Agentes Rickettsiais (Rickettsiales: Anaplasmataceae) em Cervo-do-pantanal (Blastocerus dichotomus) utilizando métodos sorológico e molecular

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

La molecola IRTA1 (immunoglobulin superfamily receptor trans location associated 1) risulta selettivamente espressa nei linfomi non Hodgkin B della zona marginale

La diagnosi di linfoma non Hodgkin B della zona marginale si basa su criteri morfologici e sulla sostanziale negatività per marcatori immunoistochimici espressi in altri sottotipi di linfoma B. L’ obiettivo di questo lavoro è stato, quindi, quello di ricercare una molecola specifica associata ai linfomi della zona marginale. Materiali e Metodi. Sono stati esaminati 2.104 linfomi periferici di entità nosologia eterogenea mediante un anticorpo monoclonale, diretto contro la molecola IRTA1, che riconosce la zona marginale nei tessuti linfoidi umani. Risultati. Si è riscontrata espressione di IRTA1 nel 93% dei linfomi della zona marginale ad insorgenza extranodale e nel 74% di quelli primitivi linfonodali suggerendo la possibilità che questi linfomi possano originare dalle cellule perifollicolari o monocitoidi IRTA1+ riscontrabili nei linfonodi reattivi. La valutazione immunoistochimica mediante doppia colorazione (IRTA1/bcl6), ha inoltre dimostrato come vi sia una modulazione fenotipica nelle cellule marginali neoplastiche nel momento in cui esse colonizzano i follicoli linfoidi e durante la loro circolazione nei centri germinativi. Le cellule marginali neoplastiche che differenziano in senso plasmacellulare perdono l’ espressione di IRTA1 Discussione. In conclusione, tali evidenze hanno permesso di ampliare la conoscenza sulla biologia dei linfomi marginali e sottolineano come IRTA1 sia il primo marcatore diagnostico positivo per queste neoplasie.

Cloning and expression of the gene encoding the major surface protein 5 (MSP5) of Anaplasma phagocytophilum and potential application for serodiagnosis

BACKGROUND: Anaplasma phagocytophilum (formerly known as the human granulocytic ehrlichia, Ehrlichia equi and Ehrlichia phagocytophila) is an obligate intracellular organism causing clinical disease in humans and various species of domestic animals. OBJECTIVES: The objectives of this investigation were to sequence and clone the major surface protein 5 (MSP5) of A phagocytophilum and to evaluate the suitability of this antigen in the serologic diagnosis of anaplasmosis in humans and dogs. METHODS: The msp5 gene of A phagocytophilum was sequenced, cloned, and expressed in Escherichia coli. The predicted amino acid sequence homology of the various MSP5/major antigenic protein 2 orthologs was compared among various Anaplasma and Ehrlichia species. Recombinant MSP5 of A phagocytophilum was used in an ELISA to detect antibodies in serum samples from humans and dogs infected with the organism. RESULTS: Serum samples from 104 individuals previously diagnosed with A phagocytophilum infection, as well as samples from clinically healthy humans, were tested. In addition, multiple samples from 4 dogs experimentally infected with 2 different geographic isolates of A phagocytophilum and 5 dogs naturally infected with a Swiss isolate were tested using ELISA. Using this group of immunofluorescent antibody test-positive and immunofluorescent antibody test-negative samples, we found the overall agreement between assays to be >90%. CONCLUSIONS: These results indicate that recombinant MSP5 has potential for use as a diagnostic test antigen to detect infection with A phagocytophilum in both dogs and humans. However, sequence similarities among orthologs of MSP5 in related species of anaplasma and ehrlichia suggest that cross-reactivity among these pathogens is likely if the entire peptide is used as a test antigen.

Anaplasma phagocytophilum DNA amplified from lesional skin of seropositive dogs.

Canine granulocytic anaplasmosis (CGA) is caused by the rickettsial microorganism Anaplasma phagocytophilum. CGA is typically characterized by fever, thrombocytopenia, lethargy, anorexia, arthropy, and other nonspecific clinical signs. Skin lesions have been described in naturally infected lambs and humans. The pathophysiology of CGA is not entirely clear, and the persistence of the organism after the resolution of clinical signs has been described. The aim of the study was to investigate if A. phagocytophilum can be detected in canine lesional skin biopsies from A. phagocytophilum-seropositive dogs with etiologically unclear skin lesions that improved after the treatment with doxycycline. Paraffin-embedded lesional skin biopsies were allocated into separate groups: biopsies from A. phagocytophilum-seropositive dogs responsive to treatment with doxycycline (n=12), biopsies from A. phagocytophilum-seronegative dogs (n=2), and biopsies in which skin lesions histopathologically resembled a tick bite (n=10). The serological status of the latter group was unknown. Histology of the seropositive and seronegative dog skin lesions did not indicate an etiology. DNA was extracted, and a conventional PCR for partial 16S rRNA gene was performed. Anaplasma phagocytophilum DNA was amplified from 4/12 seropositive dogs' skin biopsies. All sequences were 100% identical to the prototype A. phagocytophilum human strain (GenBank accession number U02521). Anaplasma phagocytophilum was not amplified from the 2 seronegative and 10 suspected tick bite dogs. Serum antibody titers of the PCR-positive dogs ranged from 1:200 to 1:2048. Histopathologically, a mild-to-moderate perivascular to interstitial dermatitis composed of a mixed cellular infiltrate and mild-to-moderate edema was seen in all seropositive dogs. In 8/12 seropositive dogs, vascular changes as vasculopathy, fibrinoid necrosis of the vessel walls, and leukocytoclastic changes were observed. In summary, our results support the hypothesis that the persistence of A. phagocytophilum in the skin may be causative for otherwise unexplained skin lesions in seropositive dogs.

Infection with Anaplasma phagocytophilum activates the phosphatidylinositol 3-Kinase/Akt and NF-κB survival pathways in neutrophil granulocytes

Anaplasma phagocytophilum, a Gram-negative, obligate intracellular bacterium infects primarily neutrophil granulocytes. Infection with A. phagocytophilum leads to inhibition of neutrophil apoptosis and consequently contributes to the longevity of the host cells. Previous studies demonstrated that the infection inhibits the executionary apoptotic machinery in neutrophils. However, little attempt has been made to explore which survival signals are modulated by the pathogen. The aim of the present study was to clarify whether the phosphatidylinositol 3-kinase (PI3K)/Akt and NF-?B signaling pathways, which are considered as important survival pathways in neutrophils, are involved in A. phagocytophilum-induced apoptosis delay. Our data show that infection of neutrophils with A. phagocytophilum activates the PI3K/Akt pathway and suggest that this pathway, which in turn maintains the expression of the antiapoptotic protein Mcl-1, contributes to the infection-induced apoptosis delay. In addition, the PI3K/Akt pathway is involved in the activation of NF-?B in A. phagocytophilum-infected neutrophils. Activation of NF-?B leads to the release of interleukin-8 (IL-8) from infected neutrophils, which, in an autocrine manner, delays neutrophil apoptosis. In addition, enhanced expression of the antiapoptotic protein cIAP2 was observed in A. phagocytophilum-infected neutrophils. Taken together, the data indicate that upstream of the apoptotic cascade, signaling via the PI3K/Akt pathway plays a major role for apoptosis delay in A. phagocytophilum-infected neutrophils.

Analyse exploratoire trimestrielle du marché canadien sur la valeur marginale que place l'investisseur à l'ajout d'1$ d'encaisse (trésorerie)

Cette recherche a pour objectif de mesurer la valeur marginale estimée par les investisseurs canadiens à la suite de l’ajout de 1 dollar d’encaisse, pour la période trimestrielle s’étalant de 2001 à 2013. Autrement dit, nous tentons d’évaluer si l’investisseur déprécie, estime à sa juste valeur nominale ou apprécie la valeur de 1 dollar de trésorerie, et ce à travers son impact sur la valeur marchande de l’équité. Parallèlement, nous tentons d’analyser l’influence des variables liées à la rentabilité, le financement, la croissance et le risque sur la valeur que place l’investisseur à l’ajout de 1 dollar d’encaisse. L’effet sectoriel sera notamment exploré sur l’ensemble de ces périodes. Des études passées ont démontré qu’il existe une asymétrie entre la valeur nominale de 1 dollar d’encaisse et celle estimée par les investisseurs. Plus précisément que l’ajout de 1 dollar de liquidité est évalué à prime. Somme toute, ces analyses affinent leurs résultats en tenant compte de l’influence des autres variables telles que mentionnées plus haut, afin de déterminer celles qui entraînent une appréciation de l’encaisse de celles qui conduisent à sa dépréciation. Nous avons conclu qu’en moyenne la valeur marginale de 1 dollar de liquidité est évaluée à 2,13$ par les investisseurs. Nous avons également pu prouver que les anticipations des investisseurs et le risque total influencent significativement cette estimation. Pour tenter de mesurer la valeur que place l’investisseur à l’ajout de 1 dollar d’encaisse, nous avons utilisé la méthodologie de Pinkowitz et Williamson (2002). Ce faisant, nous avons dans un premier temps mesuré l’impact net de l’encaisse sur la valeur marchande de l’équité en élimant du modèle les variables tenant compte des variations trimestrielles t-2 et t+2. L’hypothèse à vérifier en ce qui concerne l’impact net de l’encaisse est qu’il existe une asymétrie significative entre la valeur de 1 dollar et celle estimée par l’investisseur qui n’est pas uniquement provoquée par l’influence des variations passées et futures de l’encaisse. Les résultats obtenus nous ont permis de prouver que la valeur de 1 dollar d’encaisse est appréciée par l’investisseur, et ce malgré la suppression ou la présence des variables tenant compte des variations. Dans la même ligné, nous avons pu démontrer que, conjointement à l’encaisse au temps t, seules les variations liées aux anticipations des investisseurs impactaient significativement la valeur marchande de l’équité. En ce qui concerne le risque dont l’analyse s’est effectuée en dissociant le risque systématique du risque systémique. L’hypothèse à vérifier est qu’une augmentation du risque systématique entraîne une augmentation de la valeur de l’encaisse estimée par l’investisseur. Les résultats obtenus viennent appuyer cette affirmation. Quant au risque systémique, l’hypothèse à vérifier tente à affirmer qu’en période de crise l’encaisse est sous-estimée par l’investisseur et son impact sur la VME non significatif. Nos résultats nous conduisent à démontrer que pour la crise financière de 2006, cette hypothèse est justifiée, mais nous ne permettent pas de la vérifier pour chaque crise. Finalement, nous tenions à vérifier que dépendamment du secteur étudié, l’encaisse pouvait être plus appréciée par les investisseurs. Or, nos résultats ont montré qu’en présence de la liquidité aucun secteur ne se distingue significativement de ses pairs.

Ocorrência da infecção por Ehrlichia spp e Anaplasma platys em canídeos e felídeos selvagens mantidos em cativeiro no Distrito Federal e Goiás

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, Programa de Pós-Graduação em Saúde Animal, 2015.

Gene expression profile suggests that pigs (Sus scrofa) are susceptible to Anaplasma phagocytophilum but control infection

BACKGROUND Anaplasma phagocytophilum infects a wide variety of hosts and causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever in ruminants. Infection with A. phagocytophilum results in the modification of host gene expression and immune response. The objective of this research was to characterize gene expression in pigs (Sus scrofa) naturally and experimentally infected with A. phagocytophilum trying to identify mechanisms that help to explain low infection prevalence in this species. RESULTS For gene expression analysis in naturally infected pigs, microarray hybridization was used. The expression of differentially expressed immune response genes was analyzed by real-time RT-PCR in naturally and experimentally infected pigs. Results suggested that A. phagocytophilum infection affected cytoskeleton rearrangement and increased both innate and adaptive immune responses by up regulation of interleukin 1 receptor accessory protein-like 1 (IL1RAPL1), T-cell receptor alpha chain (TCR-alpha), thrombospondin 4 (TSP-4) and Gap junction protein alpha 1 (GJA1) genes. Higher serum levels of IL-1 beta, IL-8 and TNF-alpha in infected pigs when compared to controls supported data obtained at the mRNA level. CONCLUSIONS These results suggested that pigs are susceptible to A. phagocytophilum but control infection, particularly through activation of innate immune responses, phagocytosis and autophagy. This fact may account for the low infection prevalence detected in pigs in some regions and thus their low or no impact as a reservoir host for this pathogen. These results advanced our understanding of the molecular mechanisms at the host-pathogen interface and suggested a role for newly reported genes in the protection of pigs against A. phagocytophilum.

La poesia e l'infanzia: incontri, intrecci, possibilità nella terra marginale della "parola magica"

Il presente lavoro di tesi, inscritto in un’ottica pedagogica interdisciplinare che si sviluppa attraverso il paradigma indiziario, si propone di svolgere una riflessione attorno alla «parola poetica» nell’ottica di un’educazione estetica dell’infanzia che passi anche dalla scoperta di un linguaggio polisemico, metaforico, complesso, lieve quale quello poetico. Concetti quali la marginalità e l’alterità del dire poetico e della figura stessa del poeta, che dà voce al momento liminale della communitas, si fanno rivelatori di quella che si mostra come vera e propria “parola magica” capace di dare senso, nel mito e nel rito che costantemente lo rigenera, all’esperienza umana. La ricerca prende quindi in esame alcuni momenti paradigmatici, nella storia dell’immaginario e nella riflessione letteraria occidentale, che hanno favorito l’incontro fra poesia e infanzia nel terreno di una parola che dà voce alla liminalità: da Rousseau a Pascoli, da Baudelaire a Stevenson, numerosi sono gli autori che conducono la riflessione fino a quel territorio di soglia proficuamente spaesante che è la poesia «autentica» per l’infanzia contemporanea. In particolare, questa ricerca prende in esame la collana di poesia della casa editrice Topipittori significativamente denominata “Parola magica”. Ventidue titoli, riconducibili alle due tipologie testuali che C. Boutevin definisce «raccolta di poesie illustrata» e «albo-poesia», permettono un affondo nella poesia contemporanea per l’infanzia, che esprime in questa collana la propria dimensione di soglia: l’interdipendenza fra parola e immagine e la diffusa presenza di «tracce di fiaba» ne sono i principali indizi analizzati in questo lavoro.

Francisella-like endosymbionts of ticks

Ticks affect human and animal health both directly by their blood feeding and indirectly by transmission of many disease-causing bacteria, such as Rickettsia, Ehrlichia, Borrelia, Coxiella, Cowdria, Anaplasma, Aegyptionella, and Tularemia, as well as many viruses (Piesman and Gage, 1996). In addition to these infectious agents, ticks harbor bacterial endosymbionts, such as Wolbachia persica, which was first isolated from the soft tick now classified as Argus arboreus (Suitor and Weiss, 1961).