Análises clínicas, radiográfica e microscópica de molares permanentes sem ou com lesão de cárie na superfície oclusal

O presente trabalho, objetivou avaliar comparativamente o diagnóstico clínico, radiógráfico e histológico de molares permanentes humanos sem ou com lesão de cárie na superfície oclusa!. Vinte e quatro dentes que estavam armazenados em formalina a 10% foram selecionados e em seguida submetidos a um exame clínico, através da inspeção visual, onde foram separados em 3 grupos: grupo 1 (G1), formado por dentes hígidos; grupo 2(G2), por dentes com pigmento na superfície oclusal e grupo 3(G3), aqueles com lesão de cárie na oclusal. Este exame foi realizado com auxílio de luz artificial proveniente de um refletor odontológico, sem qualquer manipulação da superfície. Para cada dente foram realizados duas tomadas radiográficas com e sem tela milimetrada, no sentido vestibulo lingual por um dos avaliadores, simulando uma radiografia interproximal, onde o feixe incidiu perpendicular ao longo eixo do dente. Em seguida os dentes foram preparados histomorfologicamente para coloração com hematoxilina e eosina e análise microscópica. Pela avaliação dos resultados a partir da amostra de 100%, foi possível concluir: 1) Os dentes clinicamente considerados hígidos (G1) apresentaram radiograficamente 74,99% de ausência de imagem radiolúcida e em 25% presença desta imagem na Junção Amelo¬dentinária e microscopicamente ausência de cárie em 100%; 2) os dentes que clinicamente apresentaram fissura pigmentada' (G2) notou-se em 33,33%, ausência da imagem radiolúcida e em 66,66% presente esta imagem na junção amelodentinária, 100% de ausência de cárie através da análise microscópica; 3) clinicamente, os dentes que apresentaram lesão de cárie (G3), radiograficamente foram comprovados 100% de imagem radiolúcida sugestiva de cárie e microscopicamente dos 100% destas, foram considerados cáries rasa (16,66%), média (61,10%) e profunda (22,22%) respectivamente

Análises estratigráfica e estrutural da Seção Rifte (Valanginiano ao Barremiano) na área do levantamento sísmico 3D de baixo vermelho, Bacia Potiguar emersa

Baixo Vermelho area, situated on the northern portion of Umbuzeiro Graben (onshore Potiguar Basin), represents a typical example of a rift basin, characterized, in subsurface, by the sedimentary rift sequence, correlated to Pendência Formation (Valanginian-Barremian), and by the Carnaubais fault system. In this context, two main goals, the stratigraphic and the structural analysis, had guided the research. For this purpose, it was used the 3D seismic volume and eight wells located in the study area and adjacencies. The stratigraphic analysis of the Valanginian-Barremian interval was carried through in two distinct phases, 1D and 2D, in which the basic concepts of the sequence stratigraphy had been adapted. In these phases, the individual analysis of each well and the correlation between them, allowed to recognize the main lithofacies, to interpret the effective depositional systems and to identify the genetic units and key-surfaces of chronostratigraphic character. The analyzed lithofacies are represented predominantly by conglomerates, sandstones, siltites and shales, with carbonate rocks and marls occurring subordinately. According to these lithofacies associations, it is possible to interpret the following depositional systems: alluvial fan, fluvio-deltaic and lacustrine depositional systems. The alluvial fan system is mainly composed by conglomerates deposits, which had developed, preferentially in the south portion of the area, being directly associated to Carnaubais fault system. The fluvial-deltaic system, in turn, was mainly developed in the northwest portion of the area, at the flexural edge, being characterized by coarse sandstones with shales and siltites intercalated. On the other hand, the lacustrine system, the most dominant one in the study area, is formed mainly by shales that could occur intercalated with thin layers of fine to very fine sandstones, interpreted as turbidite deposits. The recognized sequence stratigraphy units in the wells are represented by parasequence sets, systems tracts and depositional sequences. The parasequence sets, which are progradational or retrogradational, had been grouped and related to the systems tracts. The predominance of the progradation parasequence sets (general trend with coarsening-upward) characterizes the Regressive Systems Tract, while the occurrence, more frequently, of the retrogradation parasequence sets (general trend with finning-upward) represents the Transgressive System Tract. In the seismic stratigraphic analysis, the lithofacies described in the wells had been related to chaotic, progradational and parallel/subparallel seismic facies, which are associated, frequently, to the alluvial fans, fluvial-deltaic and lacustrine depositional systems, respectively. In this analysis, it was possible to recognize fifteen seismic horizons that correspond to sequence boundaries and to maximum flooding surfaces, which separates Transgressive to Regressive systems tracts. The recognition of transgressive-regressive cycles allowed to identify nine, possibly, 3a order deposicional sequences, related to the tectonic-sedimentary cycles. The structural analysis, in turn, was done at Baixo Vermelho seismic volume, which shows, clearly, the structural complexity printed in the area, mainly related to Carnaubais fault system, acting as an important fault system of the rift edge. This fault system is characterized by a main arrangement of normal faults with trend NE-SO, where Carnaubais Fault represents the maximum expression of these lineations. Carnaubais Fault corresponds to a fault with typically listric geometry, with general trend N70°E, dipping to northwest. It is observed, throughout all the seismic volume, with variations in its surface, which had conditioned, in its evolutive stages, the formation of innumerable structural features that normally are identified in Pendencia Formation. In this unit, part of these features is related to the formation of longitudinal foldings (rollover structures and distentional folding associated), originated by the displacement of the main fault plan, propitiating variations in geometry and thickness of the adjacent layers, which had been deposited at the same time. Other structural features are related to the secondary faultings, which could be synthetic or antithetic to Carnaubais Fault. In a general way, these faults have limited lateral continuity, with listric planar format and, apparently, they play the role of the accomodation of the distentional deformation printed in the area. Thus, the interaction between the stratigraphic and structural analysis, based on an excellent quality of the used data, allowed to get one better agreement on the tectonicsedimentary evolution of the Valanginian-Barremian interval (Pendência Formation) in the studied area

Informações nutricionais de carnes ovinas em rótulos comerciais, comparativamente às obtidas em análises laboratoriais

Objetivou-se estudar as informações nutricionais de carnes ovinas comercializadas, comparativamente às determinadas por análises laboratoriais, utilizando-se 18 ovinos Ile de France x Ideal (cordeiros não castrados, ovelhas adultas de descarte e capões adultos de descarte). Os cordeiros foram desmamados com 17 kg e abatidos aos 32 kg. As ovelhas e capões, com 55 kg. As informações nutricionais dos rótulos de carne ovina embalada foram obtidas através do contato direto com 19 empresas que comercializam carne ovina. A maioria das empresas avaliadas não utiliza a informação nutricional na rotulagem da carne ovina embalada e também não descrimina a categoria ovina dos cortes da carcaça disponibilizados para venda. Os valores das informações nutricionais, analisadas laboratorialmente, da carne ovina evidenciaram, de modo geral, diferenças entre as categorias ovinas e seus respectivos cortes da carcaça para: valor calórico, teor de carboidratos, proteínas, gorduras totais, colesterol, cálcio e ferro. As empresas estudadas, de maneira geral, desconhecem ou ignoram tais diferenças entre as categorias e os cortes da carcaça ovina quanto às informações nutricionais, sendo necessário melhorar os rótulos comerciais dos cortes da carcaça ovina, a fim de informar o consumidor, de maneira mais simples, sobre o produto que está sendo adquirido para o consumo.

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

This paper provides a review on the latest advances and applications of the luminescence spectroscopy for the development of pharmaceuticals analyses methods, basically based on the photo- and chemiluminescence. The different forms of the drugs determination on pharmaceuticals through the fluorescence and chemiluminescence are discussed. The analyses include the drugs native fluorescence (liquid and solid-phases); the fluorescence from the oxidizing or reducing forms of the drug; the fluorescence from the chemical derivatization and their photochemistry and hydrolysis reactions. The quenching of luminescence and chemiluminescence generation for the pharmaceutical quantification are also shown. Finally, the trends and future perspectives of the luminescence spectroscopy in the field of the pharmaceutical research are discussed.

Potencialidades e aplicações do ligante dietilditiofosfato de amônio em análises químicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análises macro e microscópicas de enxertos cutâneos por semeadura após laserterapia de baixa intensidade

OBJETIVO: observar se a laserterapia de baixa intensidade acelera o processo inflamatório, a cicatrização e epitelização de enxertos cutâneos por semeadura. MÉTODOS: vinte ratos foram submetidos a esta técnica de enxertia e divididos em dois grupos iguais, um tratado com laser e outro controle. RESULTADOS: houve menor tempo de reação inflamatória, maior velocidade de cicatrização, epitelização e queratinização nos animais tratados com laser em relação aos não tratados. CONCLUSÃO: a laserterapia de baixa intensidade é efetiva no auxílio ao tratamento de enxertos por semeadura.

Comparação entre análises para permanência no rebanho de vacas Nelore utilizando modelo linear e modelo de limiar

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Merleau-Ponty e a psicologia infantil: análises da psicogênese em Wallon

Neste artigo tratamos das análises do filósofo Maurice Merleau-Ponty acerca das teorias da psicogênese de Henri Wallon. Focalizamos os cursos que Merleau-Ponty dedicara à psicologia da criança entre 1949 e 1952. Para o filósofo, ao longo da psicogênese, a distinção do indivíduo em relação ao mundo e a outrem jamais é terminada. A infância jamais é liquidada radicalmente, pelo simples fato de que não abandonamos nossa condição corporal, nosso poder de retomar as condutas e expressões fisionômicas dos outros e das coisas. Merleau-Ponty encontra indicações neste sentido nas teorias de Wallon, principalmente nos conceitos de sociabilidade sincrética e de ultracoisas.

Fezes de galinhas poedeiras na alimentação de ruminantes análises microbiológicas

The objectives of the present study were: to evaluate the variation in total number of microorganisms in laying hen fecal samples and to identify the pathogenic microorganisms possibly present such as Staphylococcus aureus, Salmonella sp., Clostridium botulinum, C chauvoei, Campylobacter sp., Escherichia coli, and Corynebacterium sp. The experiment was conducted at the School of Agrarian and Veterinarian Sciences of Jaboticabal, - UNESP. Fecal samples were collected from the aviary. A fully randomized experimental design was used with 7 treatments and 4 replications, for a total of 28 samples. A decrease in total number of microorganisms was detected when treatment T0 (zero days of storage) was compared with treatment T6 (42 days of storage), with oscillations in numbers being observed during this period of time. Pathogenic bacteria such as Clostridium noyvi, C perfringens, C sordelli, C septicum and C noyvi type B were detected, as well as non-pathogenic bacteria such as Bacillus sp. and Staphylococcus epidermidis.

Variação genética para compostos bioquímicos em sementes de duas populações naturais de Genipa americana L.: 1-análises individuais e univariada

Brazil is one of the world's richest countries in forest biodiversity. This has a great importance for humanity for sheltering essential scientific potential for the improvement in the quality of living beings. It also contributes to the preservation of natural resources and the environment. Among Brazil's forest species, jenipapo (Genipa americana L.) is commonly found in riparian vegetation. The objective of this work was to quantify the genetic variability of biochemical traits of seeds, as a support for in situ and ex situ conservation of natural populations of G. americana L. Two populations of the species were studied: one from Ilha Solteira, SP region (ISA) and other from Mogi Guaçu, SP (MOG). In the ISA population, seeds were collected from 30 trees, and in the MOG population, from 22 trees. These seeds were analyzed for chemical composition: content of proteins, carbohydrates, lipids and starch. The two populations of G. americana studied had a great genetic variation for these traits. The population ISA had an additive and phenotypic correlation, which was negative and high for starch with lipid; and positive and medium, for lipid with prolamine, as well as for globulin with prolamine. The population MOG presented additive and phenotypic correlations: positive and medium for gluteline with globulin. In these populations, narrow sense heritability estimates of progenies were moderate (0.69 for carbohydrates in the population ISA) to high (0.81 to 0.99 for the other traits in the two populations), indicating that much progress can be expected with selection strategies.

Seleção de genótipos resistentes de amendoinzeiro a Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) com base em análises multivariadas

The velvetbean caterpillar Anticarsia gemmatalis Hübner attacks peanut leaves, and the use of resistant varieties has directly contributed to ecological and economic aspects of pest control. The aim of this work was to select resistant peanut genotypes to A. gemmatalis using cluster analyses (dendogram obtained by Ward's methods and K-means) and Principal Components analysis for data interpretation. The evaluated genotypes were: IAC 5, IAC 8112, IAC 22 and IAC Tatu ST with upright growth habit, and IAC 147, IAC 125, IAC Caiapó and IAC Runner 886 with runner growth habit, and soybean genotype BR 16 as a susceptible control. The biological parameters: leaf consumption, larval (4o instar) and pupal (24h old) weight, larval and pupal development time and adult longevity were evaluated at laboratory conditions. The genotypes IAC 147 and IAC Runner 886 were resistant to A. gemmatalis in both cluster tests, grouping apart from most of the other genotypes. Both dendrogram and K-means methods provided satisfactory biological explanation, and they can be complementary used together with Principal Component and vice-versa. These results suggest that cluster analyses may be an important statistical tool in the selection of host plant resistance.

Determinação da toxicidade do selênio por meio de análises hematológicas em Oreochromis niloticus

Selenium (Se) is described as an essential micronutrient and participates in different biological functions, as the antioxidant defense systems maintenance and regulation. However, when in high concentrations, Se may cause toxic effects as well as hematological changes in fish. The aim of the present study was to determine the toxicity of selenium in the form of sodium selenate (Na 2Se 6+O 4) in Oreochromis niloticus based on hematological parameters, after exposure to different concentrations (0.01, 0.14 and 1.4 mg Se 6+ L -1). The erythrocytic and leukocytic series were examined over 14 days at intervals of 0, 3, 5, 7,10 and 14 days. The erythrocytic series showed significant alterations in the first 7 days, including the control group. Neutrophils and monocytes showed variations in the first 3 days at a concentration of 1.40 mgSe 6+ L -1 characterizing an acute response. The total number of leukocytes was different in relation to time zero on all Se concentrations. The thrombocyte count also differed statistically from time zero and control in the first 3 days at 0.14 mgSe 6+ L -1. These results indicate that different concentrations induce an acute response with diminution of total leukocytes, neutrophilia, monocytosis and thrombocytosis.

Análises técnicas e econômicas no sistema de integração lavoura-pecuária submetido à adubação nitrogenada

Sustainable agriculture, reduced production costs and adding value to the system are achieved through the use of agricultural areas throughout the year, in integrated or mixed system. The study aimed to determine the technical coefficients to estimate and evaluate the operational cost and profitability indicators of the consortium of corn with two species of Brachiaria (B. brizantha, MG-5 and B. ruziziensis). The crops were submitted to N rates (0, 100, 200, 300 e 400kg ha-1year-1) at sidedressing, using urea, and winter common bean in succession, in crop-livestock integration under no-tillage, in irrigated conditions, in 2008/2009 agricultural year in Cerrado region (Selvíria - MS). The experiment was conducted in a randomized complete block design with four replications in a 2 x 5factorial. It was concluded thatcathe inputs were the components that most increased the producing cost of corn and common bean in the ILP study. The total operational cost has increased as a function of increasing doses of N. In all treatments the levels of profitability were positive indicating the viability of the system. The treatment that showed the best technical and economic performance was intercropped with corn and B. brizantha, fertilized with 100kg ha-1year-1N and common bean in succession.

Avaliação in vitro de diferentes métodos de análises de fungitoxicidade de óleos essenciais

Environmental problems caused by synthetic fungicides have increased the search for alternative methods of control of plant diseases. The objective was to evaluate the effect of essential oil of citronella grass, on the fungus Rhizoctonia solani, in different methods of in vitro fungitoxicity. We used a randomized design in a factorial design with four replications, where the factors were composed of four methods for assessing the in vitro fungitoxicity of the essential oil of citronella grass (essential oil diluted in Tween 80 (0.5%) and embedded in the culture medium PDA (potato dextrose agar) still melting, essential oil diluted in Tween 80 (0.5%) and distributed on the surface of the PDA; oil essential diluted in Tween 80 (0.5%) and distributed on filter paper attached to the inner surface of the lid of the Petri dish, pure essential oil and distributed on the surface of the culture medium, and control) and five evaluation periods (2, 4, 6, 8 and 10 days of incubation). Was used 0.25μL mL-1 of citronella oil in all treatments. Of the treatments evaluated the use of pure oil distributed on the surface of the culture medium was more effective in reducing the mycelial diameter in all evaluations. In this method the rate of mycelial growth was 9,02 mm day-1, reaching in last evaluation 79,77 mm.

O clima do oeste do Paraná: análises da presença do lago de Itaipu

Pós-graduação em Geografia - IGCE