(Table T2) Stable carbon and oxygen isotopes of DSDP Leg 30, Leg 33, ODP Leg 130 and Leg 192 sites, Ontong Java Plateau

Stable isotopic analyses of bulk carbonates recovered from Ontong Java Plateau during Ocean Drilling Program (ODP) Leg 192 (Holes 1183A and 1186A) show an ~0.5 per mil increase in d18O values from the upper Campanian/lower Maastrichtian to the upper Maastrichtian. This shift is consistent with widespread evidence for cooling at this time. Similar shifts were found at other localities on Ontong Java Plateau (Deep Sea Drilling Project [DSDP] Sites 288 and 289 and ODP Site 807) and at DSDP Site 317 on Manihiki Plateau. These data extend evidence for Maastrichtian cooling into the southwestern tropical and subtropical Pacific. The record of apparent cooling survives despite a significant diagenetic overprint at all sites. Comparing average Maastrichtian d18O values among sites suggests that diagenesis caused d18O to first be shifted toward higher values and then back toward lower values as burial depth increased. Carbon isotopes at the six sites show no apparent primary shifts, but at four sites, the Cretaceous/Tertiary boundary interval coincides with a negative excursion attributed to alteration of sediments near the boundary.

La edición de glosas y comentarios medievales: el caso del “Commentum Monacense” a Terencio

[ES] Los conjuntos de glosas conservados en los mss. medievales representan a menudo la culminación de sucesivos y complicados procesos de fragmentación, reagrupación o/y reelaboración. Debido a su inestabilidad, estos conjuntos constituyen composiciones únicas, por lo que a la hora de editarlos la mejor opción consiste en partir del material conservado en un solo ms., sin utilizar varios testimonios para tratar de reconstruir un texto cuya existencia es meramente conjetural. Este proceder no excluye la posibilidad de recurrir al contenido de otros mss. que transmiten glosas similares a aquellas que forman el conjunto que uno trata de editar y, de hecho, en los casos en los que el contenido de este último resulta problemático o manifiestamente defectuoso, el recurso a estos testimonios paralelos es, más que una posibilidad, una exigencia. El análisis de las dos ediciones del "Commentum Monacense" a Terencio confeccionadas hasta la fecha —la de F. Schlee y la de F. Schorsch— nos proporciona algunos ejemplos concretos al respecto.

Development of the morpholino gene knockdown technique in Fundulus heteroclitus: a tool for studying molecular mechanisms in an established environmental model.

A significant challenge in environmental toxicology is that many genetic and genomic tools available in laboratory models are not developed for commonly used environmental models. The Atlantic killifish (Fundulus heteroclitus) is one of the most studied teleost environmental models, yet few genetic or genomic tools have been developed for use in this species. The advancement of genetic and evolutionary toxicology will require that many of the tools developed in laboratory models be transferred into species more applicable to environmental toxicology. Antisense morpholino oligonucleotide (MO) gene knockdown technology has been widely utilized to study development in zebrafish and has been proven to be a powerful tool in toxicological investigations through direct manipulation of molecular pathways. To expand the utility of killifish as an environmental model, MO gene knockdown technology was adapted for use in Fundulus. Morpholino microinjection methods were altered to overcome the significant differences between these two species. Morpholino efficacy and functional duration were evaluated with molecular and phenotypic methods. A cytochrome P450-1A (CYP1A) MO was used to confirm effectiveness of the methodology. For CYP1A MO-injected embryos, a 70% reduction in CYP1A activity, a 86% reduction in total CYP1A protein, a significant increase in beta-naphthoflavone-induced teratogenicity, and estimates of functional duration (50% reduction in activity 10 dpf, and 86% reduction in total protein 12 dpf) conclusively demonstrated that MO technologies can be used effectively in killifish and will likely be just as informative as they have been in zebrafish.

Age models and stable isotope ratios from the Mid-Miocene

Mid-Miocene pelagic sedimentary sections can be correlated using intermediate and high resolution oxygen and carbon isotopic records of benthic foraminifera. Precision of a few tens of thousands of years is readily achievable at sites with high sedimentation rates, for example, Deep Sea Drilling Project sites 289 and 574. The mid-Miocene carbon isotope records are characterized by an interval of high d13C values between 17 and 13.5 Ma (the Monterey Excursion of Vincent and Berger 1985) upon which are superimposed a series of periodic or quasi-periodic fluctuations in d13C values. These fluctuations have a period of approximately 440 kyr, suggestive of the 413 kyr cycle predicted by Milankovitch theory. Vincent and Berger proposed that the Monterey Excursion was the result of increased organic carbon burial in continental margins sediments. The increased d13C values (called 13C maxima) superimposed on the generally high mid-Miocene signal coincide with increases in d18O values suggesting that periods of cooling and/or ice buildup were associated with exceptionally rapid burial of organic carbon and lowered atmospheric CO2 levels. It is likely that during the Monterey Excursion the ocean/atmosphere system became progressively more sensitive to small changes in insolation, ultimately leading to major cooling of deep water and expansion of continental ice. We have assigned an absolute chronology, based on biostratigraphic and magneto-biostratigraphic datum levels, to the isotope stratigraphy and have used that chronology to correlate unconformities, seismic reflectors, carbonate minima, and dissolution intervals. Intervals of sediment containing 13C maxima are usually better preserved than the overlying and underlying sediments, indicating that the d13C values of TCO2 in deep water and the corrosiveness of seawater are inversely correlated. This again suggests that the 13C maxima were associated with rapid burial of organic carbon and reduced levels of atmospheric CO2. The absolute chronology we have assigned to the isotopic record indicates that the major mid-Miocene deepwater cooling/ice volume expansion took 2 m.y. and was not abrupt as had been reported previously. The cooling appears abrupt at many sites because the interval is characterized by a number of dissolution intervals. The cooling was not monotonic, and the 2 m.y. interval included an episode of especially rapid cooling as well as a brief return to warmer conditions before the final phase of the cooling period. The increase in d18O values of benthic foraminifera between 14.9 and 12.9 Ma was greatest at deeper water sites and at sites closest to Antarctica. The data suggest that the d18O value of seawater increased by no more than about 1.1 per mil during this interval and that the remainder of the change in benthic d18O values resulted from cooling in Antarctic regions of deepwater formation. Equatorial planktonic foraminifera from sites 237 and 289 exhibit a series of 0.4 per mil steplike increases in d13C values. Only one of these increases in planktonic d13C is correlated with any of the features in the mid-Miocene benthic carbon isotope record.

(Table DR1) Biogenic silica and chert in the Pacific Ocean, DSDP and ODP data

Evidence for the dissolution of biogenic silica at the base of pelagic sections supports the hypothesis that much of the chert formed in the Pacific derives from the dissolution and reprecipitation of this silica by hydrothermal waters. As ocean bottom waters flow into and through the crust, they become warmer. Initially they remain less saturated with respect to dissolved silica than pore water in the overlying sediments. With the diffusion of heat, dissolved ions, and to some extent the advection of water itself, biogenic silica in the basal part of the sedimentary section is dissolved. Upon conductively cooling, these pore waters precipitate chert layers. The most common thickness for the basal silica-free zone (20 m) lies below the most common height of the top of the chert interval above basement (50 m). This mode of chert formation explains the frequent occurrence of chert layers at very shallow subbottom depths in pelagic sections of the Pacific. It is also consistent with the common occurrence of cherts

Geochemistry of planktonic foraminifera from DSDP sites in the Atlantic and Pacific Ocean

The lithium content of planktonic foraminiferal calcite has been determined to evaluate temporal variability of seawater Li concentrations over the past 116 m.y. Mean foraminiferal calcite lithium/calcium in each time interval is no more than 16% greater nor 25% less than the mean Li/Ca of all samples. Li/Ca minima are observed for samples from 50-60 m.y. and 80-90 m.y., with Li/Ca about 25% lower than in adjacent time intervals. At no time during the past 40 m.y does mean Li/Ca appear to be higher than that at present. Subject to the limitations imposed by sample coverage and diagenesis, a similar conclusion holds for the past 116 m.y. Coupled with an oceanic mass balance model for Li, these data suggest that: (1) oceanic Li concentrations and, therefore, high-temperature hydrothermal circulation fluxes during the past 40 m.y. (and perhaps the past 100 m.y.) have not been more than perhaps 30-40% greater than at present for intervals any longer than a million years at most, and (2) these fluxes were not a factor of two higher 100 m.y. ago. By inference, variations in oceanic crustal generation rates over these time periods are similarly limited. Decreases in hydrothermal circulation fluxes and crustal generation rates or fluctuations up to 20% in these rates of a few million years duration are not necessarily ruled out by the Li/Ca data. The lack of variability in Li/Ca over time is not unequivocal evidence that hydrothermal fluxes have not varied because the rates of removal processes may be linked to changes in input fluxes.

(Table 1) Geochemistry of basalts of Nauru Basin

Leg 61 of the Deep Sea Drilling Project (DSDP) was concerned with drilling a single continuously cored multiple re-entry hole at site 462 in the Central Nauru Basin (Fig. 1). Preliminary results of this drilling, which penetrated more than 1 km beneath the sea floor, were presented earlier. One major result was the discovery of a late Cretaceous off-ridge volcanic/intrusive complex of basaltic composition and great thickness (>500 m). We now present trace element abundance data for these basalts. Results of the drilling provide further support for a relatively long-lived thermal and magmatic event in the late Cretaceous resulting in voluminous and widespread magmatism in the central and western Pacific consistent with earlier suggestions. The trace element data show that most of the rocks produced during this event have trace element characteristics intermediate between those of normal and transitional mid-ocean ridge basalts (N- and T-type MORB) and different from Hawaiian basalts. These results indicate that basalts which are depleted in light rare earth elements (LREE) relative to the heavy REE may, in certain conditions, be erupted as voluminous intra-plate eruptions far from active ridge crests.

Distribution of radiolaria in Oligocene to Pleistocene sediments of the North-West Pacific

Neogene stratigraphy of the tropical and subtropical Pacific on radiolaria is studied in the book. A detailed comparison of coeval systems from tropics and subtropics is given. A possibility of use of a uniform zonal scale in these areas is proved. Magnitude of changes of complexes on borders of Neogene zones is studied in detail. Six stages in development of radiolarians are identified in the tropics in Neogene. Stratigraphic levels, where the greatest changes of fauna occurred, are natural boundaries of these stages. 72 species of radiolarians (two of which are new) are described in the book.

Radiolarian stratigraphy across the Oligocene/Miocene transition in low latitudes

Recently the International Union of Geological Sciences (Commission on Stratigraphy, Working Group on the Paleogene/Neogene Boundary) proposed that the Oligocene/Miocene boundary be placed at the base of Chron C6Cn2n at 23.8 Ma on the Cande and Kent (1992) magnetic time scale, where it is approximated by planktic foraminifera at the first occurrence of Globorotulia kugleri, and by calcareous nannofossils at the last occurrence of Sphenolithus ciperoensis and the first and last occurrences of Sphenolithus delphix and S. capricornutus. Herein we show that, in terms of radiolarians, the base of Chron C6Cn2n can be correlated with the upper part of the Lychnocanoma elongata Zone between the last occurrence of Artophormis gracilis (23.94 Ma) and the first occurrence of Cyrtocapsella tetrapera (23.69 Ma). Since the proposed stratotype at Lemme-Carrosio (Italy) does not contain radiolarians at the boundary, we re-examined 13 DSDP sites and established the stratigraphic sequence of 29 first and last radiolarian occurrences and one evolutionary transition across the boundary. Nine of these sites contain both calcareous and siliceous microfossils and thus allow for an integrated biostratigraphy. Paleomagnetic stratigraphy is not available for any of the DSDP cores examined. However, use of Hodell and Woodruff's (1994) strontium isotope curve from DSDP Site 289 has permitted calibration of several low latitude microfossil datum levels against the geomagnetic polarity scale. Two new species, Lychnocanoma apodora and Eucyrtidium plesiodiaphanes, are described.