Combination of expression levels of miR-21 and miR-126 is associated with cancer-specific survival in clear-cell renal cell carcinoma

BACKGROUND Renal cell carcinoma (RCC) is marked by high mortality rate. To date, no robust risk stratification by clinical or molecular prognosticators of cancer-specific survival (CSS) has been established for early stages. Transcriptional profiling of small non-coding RNA gene products (miRNAs) seems promising for prognostic stratification. The expression of miR-21 and miR-126 was analysed in a large cohort of RCC patients; a combined risk score (CRS)-model was constructed based on expression levels of both miRNAs. METHODS Expression of miR-21 and miR-126 was evaluated by qRT-PCR in tumour and adjacent non-neoplastic tissue in n = 139 clear cell RCC patients. Relation of miR-21 and miR-126 expression with various clinical parameters was assessed. Parameters were analysed by uni- and multivariate COX regression. A factor derived from the z-score resulting from the COX model was determined for both miRs separately and a combined risk score (CRS) was calculated multiplying the relative expression of miR-21 and miR-126 by this factor. The best fitting COX model was selected by relative goodness-of-fit with the Akaike information criterion (AIC). RESULTS RCC with and without miR-21 up- and miR-126 downregulation differed significantly in synchronous metastatic status and CSS. Upregulation of miR-21 and downregulation of miR-126 were independently prognostic. A combined risk score (CRS) based on the expression of both miRs showed high sensitivity and specificity in predicting CSS and prediction was independent from any other clinico-pathological parameter. Association of CRS with CSS was successfully validated in a testing cohort containing patients with high and low risk for progressive disease. CONCLUSIONS A combined expression level of miR-21 and miR-126 accurately predicted CSS in two independent RCC cohorts and seems feasible for clinical application in assessing prognosis.

Ms. hebr. oct. 126 - Perush masekhet Avot

Vorbesitzer: Eljāqīm Carmoly; Abraham Merzbacher

Ein Sabbathpsalm : Psalm 126

Dolorosa

Ms. lat. qu. 126 - Storia del Consiglio dei Dieci

Vorbesitzer: Johann Hieronymus Zum Jungen;

Psalm 126 : gewidmet Herrn Dr. Heinrich Loewe, der mich zum Zionismus geführt hat

Dolorosa

Ms. Barth. 126 - Doctrinale cum commento

Vorbesitzer: Brun zu Brunfels; Jungo Frosch; Brune-Kütze-Legat; Bartholomaeusstift Frankfurt am Main

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 126 - Grußnotiz aus dem Landsitz

u.a.: Heinrich Floris Schopenhauer;

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 791 - Vorverkaufsangebot ihrer Güter in Ohra

abgedruckt in: Schopenhauer-Jahrbuch 60 (1979), S. 230

Ms.Ff.F.Stoltze 3. 126 - Brief von Otto Hörth an Friedrich Stoltze

Ziegenhain, Mögliches Ende der Mitarbeit an der Frankfurter Latern, wegen Briefzensur

Ms. Praed. 126 - Sermones super Magnificat

Vorbesitzer: Dominikanerkloster Frankfurt am Main

Ms. Ff. Mus. 791 - Dom. Reminiscere

Telemann. [Text: Gottfried Simonis]

Ms. lat. oct. 126 - Vita Jesu Christi

Vorbesitzer: Hilarion a S. Francisco