Combination of expression levels of miR-21 and miR-126 is associated with cancer-specific survival in clear-cell renal cell carcinoma

BACKGROUND Renal cell carcinoma (RCC) is marked by high mortality rate. To date, no robust risk stratification by clinical or molecular prognosticators of cancer-specific survival (CSS) has been established for early stages. Transcriptional profiling of small non-coding RNA gene products (miRNAs) seems promising for prognostic stratification. The expression of miR-21 and miR-126 was analysed in a large cohort of RCC patients; a combined risk score (CRS)-model was constructed based on expression levels of both miRNAs. METHODS Expression of miR-21 and miR-126 was evaluated by qRT-PCR in tumour and adjacent non-neoplastic tissue in n = 139 clear cell RCC patients. Relation of miR-21 and miR-126 expression with various clinical parameters was assessed. Parameters were analysed by uni- and multivariate COX regression. A factor derived from the z-score resulting from the COX model was determined for both miRs separately and a combined risk score (CRS) was calculated multiplying the relative expression of miR-21 and miR-126 by this factor. The best fitting COX model was selected by relative goodness-of-fit with the Akaike information criterion (AIC). RESULTS RCC with and without miR-21 up- and miR-126 downregulation differed significantly in synchronous metastatic status and CSS. Upregulation of miR-21 and downregulation of miR-126 were independently prognostic. A combined risk score (CRS) based on the expression of both miRs showed high sensitivity and specificity in predicting CSS and prediction was independent from any other clinico-pathological parameter. Association of CRS with CSS was successfully validated in a testing cohort containing patients with high and low risk for progressive disease. CONCLUSIONS A combined expression level of miR-21 and miR-126 accurately predicted CSS in two independent RCC cohorts and seems feasible for clinical application in assessing prognosis.

Ms. hebr. oct. 126 - Perush masekhet Avot

Vorbesitzer: Eljāqīm Carmoly; Abraham Merzbacher

Ein Sabbathpsalm : Psalm 126

Dolorosa

Ms. lat. qu. 126 - Storia del Consiglio dei Dieci

Vorbesitzer: Johann Hieronymus Zum Jungen;

Psalm 126 : gewidmet Herrn Dr. Heinrich Loewe, der mich zum Zionismus geführt hat

Dolorosa

Ms. Barth. 126 - Doctrinale cum commento

Vorbesitzer: Brun zu Brunfels; Jungo Frosch; Brune-Kütze-Legat; Bartholomaeusstift Frankfurt am Main

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 126 - Grußnotiz aus dem Landsitz

u.a.: Heinrich Floris Schopenhauer;

Ms.Ff.F.Stoltze 3. 126 - Brief von Otto Hörth an Friedrich Stoltze

Ziegenhain, Mögliches Ende der Mitarbeit an der Frankfurter Latern, wegen Briefzensur

Ms. Praed. 126 - Sermones super Magnificat

Vorbesitzer: Dominikanerkloster Frankfurt am Main

Ms. lat. oct. 126 - Vita Jesu Christi

Vorbesitzer: Hilarion a S. Francisco

(Table 1) Sulphur concentration and isotopic composition of ODP Leg 126 igneous rocks

Sulfur isotope ratios have been determined in 19 selected igneous rocks from Leg 126. The d34S of the analyzed rocks ranges from -0.1 â to +19.60 â. The overall variation in sulfur isotope composition of the rocks is caused by varying degrees of seawater alteration. Most of the samples are altered by seawater and only five of them are considered to have maintained their magmatic sulfur isotope composition. These samples are all from the backarc sites and have d34S values varying from +0.2 â to +1.6 â, of which the high d34S values suggest that the earliest magmas in the rift are more arc-like in their sulfur isotope composition than the later magmas. The d34S values from the forearc sites are similar to or heavier than the sulfur isotope composition of the present arc.