Nonlinearity in genetic decoding: Homologous DNA replicase genes use alternatives of transcriptional slippage or translational frameshifting

The τ and γ subunits of DNA polymerase III are both encoded by a single gene in Escherichia coli and Thermus thermophilus. γ is two-thirds the size of τ and shares virtually all its amino acid sequence with τ. E. coli and T. thermophilus have evolved very different mechanisms for setting the approximate 1:1 ratio between τ and γ. Both mechanisms put ribosomes into alternate reading frames so that stop codons in the new frame serve to make the smaller γ protein. In E. coli, ≈50% of initiating ribosomes translate the dnaX mRNA conventionally to give τ, but the other 50% shift into the −1 reading frame at a specific site (A AAA AAG) in the mRNA to produce γ. In T. thermophilus ribosomal frameshifting is not required: the dnaX mRNA is a heterogeneous population of molecules with different numbers of A residues arising from transcriptional slippage on a run of nine T residues in the DNA template. Translation of the subpopulation containing nine As (or +/− multiples of three As) yields τ. The rest of the population of mRNAs (containing nine +/− nonmultiples of three As) puts ribosomes into the alternate reading frames to produce the γ protein(s). It is surprising that two rather similar dnaX sequences in E. coli and T. thermophilus lead to very different mechanisms of expression.

From pre-Hispanic to future conservation alternatives: Lessons from Mexico

In this paper, we review some past and present trends in biodiversity conservation in Mexico and explore possible explanations of why, in spite of this long history of depredation and ineffective conservation policies, the ecosystems have been able to cope with and retain most of their biological components. We suggest a hypothesis based on the persistence of a complex mosaic of past and present traditional land uses as a possible explanation for this resilience. We propose an agenda for the scope of future conservation research and policy, particularly the need to take the socioeconomic context of environmental degradation into account. We put forth a series of questions that we think need to be investigated if the conservation research community is to participate in developing solutions for the future welfare of the human species and of biodiversity on earth.

Inversion of the chordate body axis: Are there alternatives?

One major morphological difference between chordates and annelids or arthropods is the opposite orientation of the nerve cord and heart. A long-standing proposal is that the chordate axis evolved by inverting the body of an ancestor with the annelid/arthropod orientation. However, the data can also be explained by a common ancestor with diffuse dorsoventral organization, followed by oppositely directed condensation of the nerve cord and relocation of the heart in the two lines.

Suppression of O-Methyltransferase Gene by Homologous Sense Transgene in Quaking Aspen Causes Red-Brown Wood Phenotypes1

Homologous sense suppression of a gene encoding lignin pathway caffeic acid O-methyltransferase (CAOMT) in the xylem of quaking aspen (Populus tremuloides Michx.) resulted in transgenic plants exhibiting novel phenotypes with either mottled or complete red-brown coloration in their woody stems. These phenotypes appeared in all independent transgenic lines regenerated with a sense CAOMT construct but were absent from all plants produced with antisense CAOMT. The CAOMT sense transgene expression was undetectable, and the endogenous CAOMT transcript levels and enzyme activity were reduced in the xylem of some transgenic lines. In contrast, the sense transgene conferred overexpression of CAOMT and significant CAOMT activity in all of the transgenic plants' leaves and sclerenchyma, where normally the expression of the endogenous CAOMT gene is negligible. Thus, our results support the notion that the occurrence of sense cosuppression depends on the degree of sequence homology and endogene expression. Furthermore, the suppression of CAOMT in the xylem resulted in the incorporation of a higher amount of coniferyl aldehyde residues into the lignin in the wood of the sense plants. Characterization of the lignins isolated from these transgenic plants revealed that a high amount of coniferyl aldehyde is the origin of the red-brown coloration—a phenotype correlated with CAOMT-deficient maize (Zea mays L.) brown-midrib mutants.

Autoconstrução em madeira - estudo de caso: Florianópolis/SC

O tema desta pesquisa consiste no desenvolvimento das técnicas de aplicação da madeira de reflorestamento como base na construção habitacional de interesse social. Considera-se suas potencialidades para a realização de uma arquitetura que seja socialmente justa, tecnicamente viável, e acessível economicamente através de uma sistemática por autoconstrução. A pesquisa apresenta, de maneira geral, o estudo e sistematização de tecnologias construtivas em madeira, aplicadas na região sul do país, em especial na cidade de Florianópolis/SC. Buscam-se, portanto, indicadores de desempenho no processo de produção da habitação, para contribuir no aperfeiçoamento dos sistemas construtivos, do ponto de vista da durabilidade, e também na proposição de novas alternativas.

Best Alternatives to Cronbach's Alpha Reliability in Realistic Conditions: Congeneric and Asymmetrical Measurements

The Cronbach's alpha is the most widely used method for estimating internal consistency reliability. This procedure has proved very resistant to the passage of time, even if its limitations are well documented and although there are better options as omega coefficient or the different versions of glb, with obvious advantages especially for applied research in which the ítems differ in quality or have skewed distributions. In this paper, using Monte Carlo simulation, the performance of these reliability coefficients under a one-dimensional model is evaluated in terms of skewness and no tau-equivalence. The results show that omega coefficient is always better choice than alpha and in the presence of skew items is preferable to use omega and glb coefficients even in small samples.

Beyond megaprojects?. Water alternatives for mass tourism in coastal Mediterranean Spain

Water availability in adequate quantities and qualities is a fundamental requirement for tourism. In the Mediterranean, one of the world’s leading tourist destinations, water availability is subject to modest and erratic precipitation figures which may decline with climate change. The tourist industry therefore may have to assure future supplies by either recurring to new technologies such as desalination or increasing efficiency in water use. A third and yet little explored alternative would be to seek for complementary of uses with irrigation, the traditional user in many coastal Mediterranean areas and holder of substantial amounts of water. In this paper we present the example of the Consorcio de Aguas de la Marina Baja to show how Benidorm, in Mediterranean Spain and one of the most important tourist centers of the Mediterranean, obtains part of its water through agreements with farmers by which these trade their water with Benidorm and other towns’ treated wastewater of enough quality to be used for irrigation, and obtain several compensations in return. The advantages and disadvantages of the water trade between farmers and tourist interests in the Benidorm area are discussed and we argue that solutions to the pending water crisis of many coastal Mediterranean tourist areas may not need to rely uniquely on expensive technologies to generate new resources but may attempt other alternatives.

Prediction of blackwood wood and pulp quality parameters using NIR-PLS-R spectral modelling : wavenumber ranges and smoothing points

Só está disponível o resumo.

Biological lectures delivered at the Marine Biological Laboratory of Wood's Holl [sic].

1895

Time factors of differentially preserved wood in two calcitic concretions in Pennsylvanian black shale from Indiana / Bertram G. Woodland -- and Catherine K. Richardson --.

v.33:no.10(1975)

Benjamin Wood, AB, 1797

This folder contains an original (20.5 x 12 in.), a typewritten copy (14.75 x 8.5 in.), and a photostat copy (21.5 x 13.75 in.) of the diploma of Benjamin Wood.

Certificate of the sale of Cyrus' freight of wood, 1793 September 9

Accounting records related to the wood brought by the sloop between May and September 1793 on three slips of paper bound with thread. The last page contains a certification signed by William Winthrop on September 9, 1793 of the sale of wood in Charlestown.

Biological lectures delivered at the Marine Biological Laboratory of Wood's Holl [sic].

1899