Relative contribution of natural productivity and compound feed to tissue growth in blue shrimp (Litopenaeus stylirostris) reared in biofloc: Assessment by C and N stable isotope ratios and effect on key digestive enzymes

The aim of this study was to assess the relative contribution of natural productivity and compound food to the growth of the juvenile blue shrimp Litopenaeus stylirostris reared in a biofloc system. Two experiments were carried out based on the same protocol with three treatments: clear water with experimental diet (CW), biofloc with experimental diet (BF) and biofloc unfed (BU). Shrimp survival was significantly higher in biofloc rearing than in CW rearing. The contribution of the biofloc to shrimp diet was estimated through measurement of carbon and nitrogen stable isotope ratios in shrimp and food sources. Different isotopic compositions between feeds were obtained by feeding natural productivity with a mixture rich in fish meal and the shrimps with a pellet containing a high level of soy protein concentrate. Using a two source one-isotope mixing model, we found that the natural productivity of the biofloc system contributed to shrimp growth at a level of 39.8% and 36.9%, for C and N, respectively. The natural food consumed by the shrimps reared in the biofloc system resulted in higher gene expression (mRNA transcript abundance) and activities of two digestive enzymes in their digestive gland: α-amylase and trypsin. The growth of shrimp biomass reared in biofloc was, on average, 4.4 times that of those grown in clear water. Our results confirmed the best survival and promoted growth of shrimps using biofloc technology and highlighted the key role of the biofloc in the nutrition of rearing shrimps. Statement of relevance In this study, we have applied an original protocol to determine the respective contribution of natural productivity and artificial feeds on the alimentation of the juvenile blue shrimp L. stylirostris reared in biofloc system by using C and N natural stable isotope analysis. Moreover, we have compared, in shrimp digestive gland, the α-amylase and trypsin enzyme activities at biochemical and molecular levels for two different shrimp rearing systems, biofloc and clear water. In our knowledge, the use of molecular tool to study the influence of biofloc consumption on digest process of shrimp was never carried out. We think that our research is new and important to increase knowledge on biofloc topic.

Mixture of time-dependent growth models with an application to blue swimmer crab length-frequency data

Understanding how aquatic species grow is fundamental in fisheries because stock assessment often relies on growth dependent statistical models. Length-frequency-based methods become important when more applicable data for growth model estimation are either not available or very expensive. In this article, we develop a new framework for growth estimation from length-frequency data using a generalized von Bertalanffy growth model (VBGM) framework that allows for time-dependent covariates to be incorporated. A finite mixture of normal distributions is used to model the length-frequency cohorts of each month with the means constrained to follow a VBGM. The variances of the finite mixture components are constrained to be a function of mean length, reducing the number of parameters and allowing for an estimate of the variance at any length. To optimize the likelihood, we use a minorization–maximization (MM) algorithm with a Nelder–Mead sub-step. This work was motivated by the decline in catches of the blue swimmer crab (BSC) (Portunus armatus) off the east coast of Queensland, Australia. We test the method with a simulation study and then apply it to the BSC fishery data.

Transient Blue Skin: Pseudochromhidrosis

Objectives: Pseudochromhidrosis is a rare condition where colours due to chromogenic microbial products or extrinsic chemicals are excreted with sweat. Chromhidrosis is the production of coloured sweat from apocrine or eccrine sweat glands. The aim of this case report is to illustrate all the steps involved in the diagnosis of pseudochromhidrosis. Materials and methods: A 17-year-old patient with pseudochromhidrosis is presented. Results: Clinical features of the patient were consistent with pseudochromhidrosis. Conclusions: The distinction between chromhidrosis and pseudochromhidrosis can be made based on a detailed history, skin biopsy and empiric treatment

Effects of density dependence, zooplankton and temperature on blue whiting Micromesistius poutassou growth

Blue whiting Micromesistius poutassou mean total length at age in the north-east Atlantic Ocean was found to vary by around ±6% during the period 2004–2011 and mean mass at age by ±22% during the years 1981–2013. Linear modelling provided strong evidence that these phenotypic growth variations can be explained by trophic conditions, mainly negative density dependence and also food availability, and a negative long-term temperature effect on asymptotic siz

SSF guidelines: towards blue growth

A consultation workshop on East Africa recognized that, if applied well, the principles of the SSF Guidelines can advance small-scale fisheries in the region.

Blue Streak

The Blue Streak, named for the Sandusky, Ohio athletic teams, opened in 1964. It was the first new wooden roller coaster at the park since the Cyclone in 1929. A classic out-and-back coaster with a fast and hilly ride, the 78-foot-high Blue Streak is a long time guest favorite. It celebrated its 50th anniversary in 2014.

Crabbing into an uncertain future: the blue swimming crab fisher in coastal town of Eastern Philippines

This research project sought to find out the socio-economic status of the small-scale fishers of the blue swimming crab (Portunuspelagicus) in Samar, considering the diminishing volume of catch of the species in the recent years. Using a blend of quantitative and qualitative methods, the study employed an interview schedule, focus group discussion (FGD) and observation in collecting data not only from the fishers but also from other sectors directly involved in the blue swimming crab industry.

The importance of novelty: Male–female interactions among blue-black grassquits in captivity

Mate choice is a primary mechanism driving the evolution of sexually selected traits such as elabo-rate displays and ornaments. In a majority of taxa studied to date, females are seen to actively sampleand evaluate multiple males, presumably to optimize mating opportunities. During this process femalesmay encounter males both familiar and novel, a distinction that might influence how mate choice pro-ceeds. Using a socially monogamous passerine, the blue-black grassquit (Volatinia jacarina), we studiedhow females respond to novel versus familiar (“paired”) males, and how encounters with novel malesinfluence subsequent interactions with their paired males. Additionally, we measured the hormonalresponse of males after visualizing their paired females interacting with novel males. We found thatfemales were attentive to novel males irrespective of these males’ phenotypic attributes, suggesting thatin these interactions novelty is highly relevant. After exposure to novel males, females tended to respondaggressively towards their paired males; by contrast, the behaviour of males towards their paired femalesdid not change. Moreover, we did not detect any hormonal responses of males to viewing their pairedfemales interacting with novel males. Together these results suggest that the distinction between famil-iarity and novelty may hold special relevance for females in mate choice, a finding that bears upon ourunderstanding of the evolution of extra-pair paternity and reproductive behaviour.