Comparação entre pericárdio bovino preservado em glicerina e malha de poliéster no reparo de falhas da parede abdominal em ratos

OBJETIVO: Comparar uma malha comercial de poliéster com o pericárdio bovino preservado em glicerina na reconstituição de defeitos da parede abdominal. MÉTODOS: Foram utilizados 30 ratos, divididos em dois grupos eqüitativos. Efetuou-se uma excisão retangular de 2,5 x 2 cm, incluindo toda a musculatura abdominal e peritônio. No grupo I a parede abdominal foi reparada com malha de poliéster e no grupo II com pericárdio bovino conservado em glicerina. Os animais foram sacrificados aos 15, 60 e 90 dias de pós-operatório, sendo o local cirúrgico avaliado macroscópica e histologicamente. RESULTADOS: Os animais do grupo I apresentaram aderências mais severas e em maior número quando comparados aos do grupo II; porém, sem comprometimento funcional. A análise histológica revelou incorporação dos tecidos aos implantes, com maior resposta fibroblástica nos animais do grupo I. CONCLUSÃO: A malha de poliéster oferece maior resistência estrutural e resposta fibroblástica mais intensa; contudo, promove grande quantidade de aderências às vísceras abdominais, quando comparada ao pericárdio bovino.

Efeito do diclofenaco de sódio na cicatrização da parede abdominal de ratos. Estudo histopatológico, da força de ruptura e do colágeno tecidual

Com o objetivo de estudar os efeitos do diclofenaco sódico sobre a cicatrização da parede abdominal de ratos, foram utilizados 80 animais da linhagem Wistar divididos em dois grupos: Grupo 1: formado por 40 animais submetidos à laparotomia mediana e à injeção intramuscular de soro fisiológico durante quatro dias. Grupo 2: formado por 40 animais submetidos à laparotomia mediana e à injeção intramuscular de diclofenaco sódico durante quatro dias. Animais de ambos os grupos foram analisados no 5°, 7°, 14° e 21° dias de pós-operatório, correspondendo, respectivamente à M1, M2, M3 e M4. em cada momento foram estudados 10 animais e os parâmetros analisados foram a evolução clínica, a força de ruptura, estudo histológico e o conteúdo de colágeno tecidual da parede abdominal. Os animais do grupo tratado apresentaram como complicações, deiscência e/ou hérnia incisional, perda ponderal e taxa de mortalidade, complicações estas não evidenciadas no grupo controle. Observamos também neste grupo, diminuição da força de ruptura no 7° e 14° dia de pós-operatório e diminuição da concentração de colágeno tecidual no 5°, 7° e 14° dia de pós-operatório. Ambos os parâmetros retornaram a valores normais no 21° dia de pós-operatório. Quanto ao estudo histológico, concluímos que a cicatriz da parede abdominal dos animais tratados com D.S. apresentam retardo do processo cicatricial em relação aos seus controles, caracterizado por uma menor fibrogênese, menor densidade de fibras colágenas, além de um número maior de complicações, como microabscessos, em torno dos fios de sutura.

O Gênero Cyathus haller: pers. (Agaricales, Basidiomycota) em áreas de caatinga do Nordeste brasileiro

Fungi of the family Nidulariaceae or bird s nest fungi present a vase-shaped basidiomata with the wall surface smooth or striated lengthwise and internal structures resembling small bird eggs in a nest, the peridioles. Among the five genera that comprise the group, the more representative is Cyathus Haller: Pers. The Brazilian Northeast region shows major importance for the world s diversity by containing a Brazilian unique and exclusively phytoecological domain, the Caatinga, which lacks particular attention regarding macrofungi. Although the gradual growth of knowledge in recent years, studies are still insipient in the Caatinga domain. Between their various vegetal formations are the Brejos de Altitude , described as islands with moist perennial vegetation, low soil fertility and annual precipitation of 900 1.300 mm. This study aimed to evaluate the taxonomic richness of Nidulariaceae fungi in Caatinga s areas of Northeastern Brazil, describing and identifying species, as well as expanding the collection of Herbarium UFRN Fungos. For description of Nidulariaceae fungi was followed the proposed by specialized literature in the group. We have found and described 10 species of the genus Cyathus, namely: C. earlei Lloyd, C. gayanus Tul. & C. Tul., C. gracilis H.J. Brodie, C. intermedius (Mont.) Tul. & C. Tul., C. limbatus Tul. & C. Tul., C. montagnei Tul. & C. Tul., C. pallidus Berk. & M.A. Curtis, C. poeppigii Tul. & C. Tul., C. striatus (Huds.) Willd. and C. tríplex Lloyd. Beyond these have been described four new species to science: Cyathus calvescens R. Cruz & Baseia sp. nov., C. hortum R. Cruz & Baseia sp. nov., C. magnomuralis R. Cruz & Baseia sp. nov. and C. parvocinereus R. Cruz & Baseia sp. nov. Two samples were identified only to genus level. No member of Mycocalia J.T. Palmer, Nidula V.S. White, Nidularia Fr. and Crucibulum Tul. & C. Tul. has been found, however the results were extremely valuable for being an unprecedented work in Caatinga, and importante tool for implementation of conservation projects and sustainable utilization of this domain

A contribuição da biotecnologia vegetal para o desenvolvimento da cajucultura familiar no município de Serra do Mel (RN)

This work focuses the familiar cajuculture at Serra of Mel (RN) that presents a geographic and climatic structure favourable to the development of the cajuculture; the interaction between the ambient factors and agriculture in the region provides a propitious environment to the culture of the cashew; the objective of present work was associate ambient, economic, social and cultural factors of this municipy with the possibility of a sustainable agriculture associated with the vegetal biotechnology

Análise fitoecológica do manguezal e ocupação das margens do estuário hipersalino apodi/mossoró (rn brasil) a biotecnologia vegetal como alternativa para a cotonicultura

The mangrove is a coastal ecosystem of the big ecological importance, showing high fragility front by natural process and the human interventions in the coastal zone. This research has objective to analyses the relation between mangrove species distribution and geochemical parameters variation of the water and soil in Apodi/Mossoro estuary, located in the Rio Grande do Norte state north coastline. The results were obtained from floristic and structural analysis of the vegetation and Quick Bird satellite images interpretation (collected in 2006 year), manipulated with ENVI 4.3 and ArcGIS 9.2 software s. This estuary was characterized by to presents a gradient of the salinity around 40 kilometers extension, finding amount between 50 and 90 g/l-1. Will be identified the formation of the mix vegetation formation in the estuary mount, where the water salinity no show express wide variation on seawater (36 g/l-1), finding species: Rhizophora mangle L., Laguncularia racemosa (L.) C. F. Gaertn, Avicennia schaueriana Stap. & Leechman e Avicennia germinans L. Along of the estuary, have a streak formation of the vegetation composed by Avicennia spp. and L. racemosa. In high estuary, where the salinities value stay above 60 g/l-1, only A. germinans predominate in dwarf form. In this sense, the salinity is as a limiting factor of stress on the mangrove vegetation as it enters the estuary, this parameter should be taken into account when drawing up management plans and environmental restoration in the estuary in question

Vasculite necrosante na glomerulonefrite difusa aguda pós-infecciosa

Os autores relatam dois casos de glomerulonefrite difusa aguda pós-infecciosa com evolução clinicomorfológica incomum. As biópsias renais mostraram alterações características de glomerulonefrite difusa aguda associada à extensa necrose fibrinóide e infiltrado inflamatório leucocitário na parede de arteríolas e artérias interlobulares. Foram também observadas crescentes. Ambos os pacientes cursaram com insuficiência renal aguda severa, sendo que um dos pacientes recuperou a função renal e outro evoluiu para insuficiência renal crônica e óbito.

A biotecnologia vegetal como alternativa para a cotonicultura familiar sustentável

This paper aims to describe the familiar cotton culture in the districts of Tangará and Triunfo Potiguar, located in the state of Rio Grande do Norte. It relates specific problems that small cotton agriculturists face in the cotton production and sale as well as their perception in relation to public policies that have been put into practice in these districts. The research revealed soils with potentiality for the cotton culture, but that are not being cultivated for anything else but subsistence, not being able to become productive, specifically through the cotton culture. That activity should be practiced so that it preserves the environment and the familiar farmers themselves, since it was verified they are constant1y exposed to health problems due to insecticides use. In what refers to fomentation policies it was observed that bureaucracy delays the liberation of the money destined to production, seeds delivery and other actions related to production and commercialization. Some procedures such as information and training about productivity development and attention with the environrnent and infrastructure are recommended. The results indicate lack of investments, rural credit and technical help, main1y in Triunfo Potiguar. There is unanimity, from the rural farmers of that district in what refers to the desire of improving the production and remaining in that activity. In this sense, the contribution of this research for the most appropriate culture production can be materialized through the technique of tissue's culture, aimed at obtaining plants that are resistant to diseases. The data indicated that the hormonal supplementations used induced regenerative calluses making in vitro morphogenesis possible in alI tested varieties. The use of the activated coal antioxidant was efficient, reducing oxidation, however not suppressing it. The results lead to an opportunity for the familiar tàrmers from Rio Grande do Norte enlarge the cotton culture, because to obtain plants that are resistant to diseases implies in insecticides reduction, propitiating smaller impact to the atmosphere and less cost

Relação da expressão de fatores de crescimento celular (IGF-1) e (SCF) com fatores prognósticos e o alvo da rapamicina em mamíferos (m-TOR) em mastocitomas cutâneos caninos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Vegetal polymer in repair of defects of the orbital floor: an experimental study in rabbits

Background: An experimental study was done to assess the ability of the vegetal polymer miniplates and screws to repair defects of the orbital floor.Methods: An artificial standard-sized defect was created in the bony floor of right orbit of 45 albino rabbits. The animals were divided into three experimental groups: control group (G1) involving animals with orbital floor defect and no treatment; titanium group (G2) containing animals with orbital floor defect repaired by titanium miniplates and screws; vegetal polymer group (G3) composed of animals with similar orbital floor defects repaired by vegetal polymer miniplates and screws. Throughout the course of the experiment, the animals were clinically evaluated. At 15, 30 and 60 days after surgery, the animals were killed. They were X-rayed immediately after the floor defect and at the moment of sacrifice. Histological and morphometric evaluation of inflammatory reaction and bone healing was done. Data were statistically evaluated.Results: No implants were extruded. Bone consolidation was similar in G2 and G3 and better than in G1 group animals. Inflammatory reaction was most pronounced in animals of G3 15 days after surgery, and it subsided over time.Conclusion: Vegetal polymer miniplates and screws induces small inflammatory reaction and had the ability to stimulate bone growth with good integration in the orbital floor defect allowing to consider the vegetal polymer adequate option to treat orbital floor defects. Future studies involving long-term follow-up and biomechanical tests to evaluate material resistance to traction are needed.

Própolis e cera vegetal na conservação de abacate Hass

The aim of this work was to evaluate the quality of Hass avocado submitted to application of propolis and vegetable wax. The selected fruits were submitted to the treatments (1) without wax and without propolis, (2) with vegetable wax, (3) propolis alcoholic extract 100%, (4) wax and etanol 70%, (5) propolis alcoholic extract 2% and wax, (6) propolis alcoholic extract 4% and wax, (7) propolis alcoholic extract 6% and wax, (8) propolis alcoholic extract 8% and wax, and (9) propolis alcoholic extract 10% and wax. The fruits were maintained under refrigeration (10 +/- 1 degrees C and 90 +/- 5% relative humidity), being appraised every three days for 15 days and more 3 days of commercialization simulation at room temperature (23 +/- 1 degrees C and 18% relative humidity). The evaluation parameters were the weight loss, respiratory activity, pH, firmness, tritable acidity (AT), soluble solids (SS), and ratio. The results of weigth loss, respiratory activity and firmness were the most significant to show the difference among the treatments. 'Hass' avocado fruits submitted the application of propolis associated the vegetable wax present smaller weight loss, CO2 production and firmness in relation to the control fruits. The fruits of the treatment with propolis alcoholic extract 2% and wax presented the most promising results.

Implantes de resina de poliuretana vegetal (Ricinus communis) na tração linear, fixação e fusão vertebral no cão: estudo experimental

Placa e espaçador de polímero derivado do óleo de mamona (PDOM) (Ricinus communis) foram avaliados clínica, radiográfica e histologicamente na tração linear, fixação e fusão vertebral cervical em 20 cães adultos, sem raça definida, pesando entre 17 e 22kg. Foram sacrificados quatro animais aos 10, 30, 60, 90 e 120 dias de pós-operatório. Após exposição da coluna cervical, por acesso ventral, o disco intervertebral de C4-C5 foi fenestrado e a abordagem ao canal medular foi feita por meio de fenda óssea. Um espaçador de PDOM foi colocado preenchendo o defeito ósseo. Os corpos vertebrais C4-C5 foram fixados com placa do mesmo material, utilizando-se dois parafusos corticais em cada corpo vertebral. Apenas um animal apresentou déficit neurológico no pós-operatório imediato. Radiograficamente as vértebras mostravam-se normais e alinhadas, sem colapso do espaço intervertebral, porém não houve neoformação óssea entre as vértebras. Ao exame mielográfico, não houve compressão da medula espinhal. Os implantes foram efetivos em manter a tração linear e fixação das vértebras cervicais e não ocorreu a fusão vertebral.

Morfoanatomia e ontogênese do fruto e semente de Vernonia platensis (Spreng.) Less. (Asteraceae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crescimento de plantas de Salvia officinalis sob ação de reguladores de crescimento vegetal

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Controle do crescimento vegetativo e floração de mangueiras cv. Kent com reguladores de crescimento vegetal

Paclobutrazol applied to the soil is used for the production management of mangoes in most of the orchards, but it presents as disadvantage the greater persistence in plant and soil. Three growth regulators (prohexadione-Ca, trinexapac-ethyl and chlormequat chloride) were foliar applied, at two dosages and compared to paclobutrazol applied as soil-drench. The experiment was conducted in the region of the Sao Francisco Valley, Petrolina, PE, from December 2007 to July 2008, in a mango orchard that has a spacing of 8 m between lines and 5 m between plants. In order to compare the effects of the treatment, data were evaluated regarding to vegetative growth (number, length and leaf number of vegetative branches) and flowering (percentage and panicles length). The combination of PBZ, soil applied with ProCa, foliar applied was the most efficient in controlling vegetative growth of branches of the mango 'Kent', but not regarding to flowering, where there were no differences between treatments.

Substratos e regulador vegetal no enraizamento de estacas de pitaya

A pitaya é uma cactácea originária das florestas tropicais americanas que produz frutos exóticos bem apreciados e comercializados, principalmente no continente asiático. Contudo, ainda é uma espécie vegetal que demanda informações técnicas de cultivo nas áreas agrícolas brasileiras, motivando pesquisas em várias áreas do conhecimento. Objetivou-se avaliar diferentes substratos no enraizamento de cladódios imersos e não imersos no regulador de crescimento, ácido naftalenacético (ANA), na formação e desenvolvimento inicial de raízes em estacas de Hylocereus undatus, resultando em mudas mais vigorosas e de melhor qualidade. O experimento foi conduzido sob ripado no Departamento de Produção Vegetal - setor de Horticultura da Faculdade de Ciências Agronômicas no campus de Botucatu - UNESP. O delineamento experimental foi o inteiramente aleatorizado em esquema fatorial 4 x 2 (substratos x condições) com quatro repetições. Após 60 dias da instalação do experimento foram avaliados: massa fresca e seca da parte aérea, massa fresca e seca das raízes e o comprimento da maior raiz. O crescimento e desenvolvimento radicular inicial de mudas não são influenciados pelo regulador vegetal ANA. Os substratos com a presença de areia são os mais adequados para a formação de mudas vigorosas e de boa qualidade. A mistura areia + esterco bovino proporciona maior acúmulo de fitomassa no sistema radicular de pitaya (H. undatus).