Determinação da sensibilidade elementar de amostras-padrão pelo método de flurescência de raios X por reflexão total

To determinate the multielemental concentrations in biological samples using the technique of total reflection X-ray fluorescence (TRFX) is essential the use of standard samples. The aim of this study is the determination of the sensitivities of the relevant elements in standard samples (Multi Element atomic spectroscopy standard solution in HNO3 I) prepared in three solutions with different and known concentrations. Based on the sensitivities of the elements contained in the multilelementar standard sample, it can estimate the sensitivity for an element found in the biological sample and consequently it is possible to determinate the concentration, even though the standard does not contain this element. The measurement of fluorescence was performed at the Brazilian Synchrotron Light Laboratory (LNLS) in Campinas

Padrão de atividade da jaguatirica (Leopardus pardalis), cutia (Dasyprocta leporina) e paca (Cuniculus paca) no sul da Amazônia brasileira

Não disponível

Clonagem de sequências codificantes de citocinas felinas para construção de curva padrão para PCR em tempo real

A quantificação de citocinas felinas possibilita uma avaliação do sistema imune do animal em diferentes doenças, permite também a identificação de diferentes fatores que alteram sua secreção, e colabora na compreensão da patogênese das enfermidades. Em humanos e camundongos essa mensuração é feita principalmente pelo método de ELISA, mas para os felinos há uma menor disponibilidade de kits específicos para determinadas citocinas, e estes possuem um custo elevado. Assim, uma alternativa é utilizar a reação de PCR em tempo real com transcrição reversa (RT-qPCR) para quantificação absoluta dos RNAs mensageiros das citocinas felinas, uma técnica bastante sensível e específica para analisar a expressão desses genes. Com esse intuito, esse trabalho teve como objetivo clonar as sequências gênicas codificantes de citocinas, para construir uma curva padrão para a qPCR. Assim, foi feita extração de RNA de amostras de sangue total de gatos domésticos, e estes foram tratados com DNAse para evitar contaminação por DNA genômico. O cDNA foi sintetizado, e amplificado com os primers específicos para cada fragmento codificante de citocina e o GAPDH felino. Cada amplicon purificado foi inserido em um plasmídeo comercial, e introduzido em bactérias E. coli cepa DH5. Os clones recombinantes foram sequenciados para confirmar a inserção do fragmento no vetor. As curvas padrão da qPCR foram construídas com cada plasmídeo recombinante numa de 106 a 101 cópias por reação. O sequenciamento mostrou que todos os clones eram semelhantes àqueles encontrados no GenBank. A eficiência das amplificações, baseado no slope das curvas padrão foram: 101,3% para GAPDH, 99,1% para IL-1, 83,2% para IL-6, 94,4% para IL-10, 105,5% para IL-12, 83,4% para IFN- e 83,8% para TNF-. O valor de r2 foi de 0,99 em todas as reações. Dessa forma, com a clonagem dos fragmentos... (Resumo completo, clicar acesso eletrônico abaixo)

Reconhecimento de contorno de edifício em imagens de alta resolução usando os momentos complexos de Zernike

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Hip Hop na aula de FLE: aprendendo e ensinando o léxico não-padrão

Appuyé sur le concept de lexiculture, proposé par R. Galisson (2000), et en reconnaissance de l’existence et de la dimension du mouvement Hip Hop en France, le but de cet article est d’examiner l’importance de cette culture et de son lexique dans le contexte français, soulignant aussi la nécessité de son insertion dans les matériels éducatifs destinés à l’enseignement du français langue étrangère (FLE). À cet effet, nous avons elaboré, à l’aide d’un système de traitement de corpus, une liste bilingue français-portugais brésilien de ce lexique, à partir des paroles des trois principaux artistes français. Il faut noter que la plupart de ces unités lexicales ne sont pas présentes dans les dictionnaires généraux bilingues, ce qui crée des difficultés aux apprenants ainsi qu’aux enseignants d’accès à ce lexique, qui coexiste avec la langue standard étudiée et qui représente une grande richesse linguistique.

Expectativas sobre os efeitos do uso de álcool e padrão de beber em alunos de ensino médio

Studies have shown that adolescents begin to make use of alcoholic beverages earlier and excessively, a behavior which has several negative consequences. Thus, the present study aims at investigating whether the expectations they have for the effects of alcohol consumption are high or low and if there is a relationship between expectation and consumption pattern. AUDIT and IECPA were applied as data collection instruments. The first indentifies the pattern of alcohol use and the second investigates the expectations the subjects have in relation to the use of alcohol. The results of this study, differently than others, do not evidence the positive relationship between binge-drinking and high expectations about the use of alcohol.

Método para reconhecimento de íris baseado na sua região interna

Recognition of individuals through the characteristics of the iris has in recent years become a well accepted technique due to both the high reliability of this procedure and the its non invasiveness. The methods used in such procedures seek information all over the iris, which depending on the algorithm used may result in high computational costs. Considering that most characteristics of the iris are in its inner region the goal of this work is to develop an algorithm for the recognition of individuals using only this region. To bring the outcome of our approach to the level of the best techniques described in the literature this technique has to be further elaborated, even so the results show a promising technique.

O bóson de Higgs no modelo padrão das interações eletrofracas: aspectos fenomenológicos do setor escalar e resultados experimentais

Pós-graduação em Física - FEG

Análise de agrupamentos para o reconhecimento de padrões de infestação de aracnídeos em zonas urbanas

Pós-graduação em Biociências - FCLAS

Segurança em redes sem fio: estudo sobre o desenvolvimento de conjuntos de dados para comparação de IDS

Pós-graduação em Engenharia Elétrica - FEIS

Análise de impactos ambientais gerados pela implantação de loteamentos fechados de alto padrão

Pós-graduação em Engenharia Civil - FEIS

Sobre o ritmo do português brasileiro: evidências de um padrão acentual

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A norma-padrão Europeia e a mudança linguística na Escola Moçambicana

In Mozambique, the portuguese language is considered the official language, second language, national language and competes with more than twenty Bantu languages spoken by the majority of population. The standard norm lose away their floor to the Mozambican Portuguese which carries own characteristics pertaining to the sociolinguistic context of the country. Schools attempt their best, but they cannot teach the European standard due the multilingual and Portuguese contact with African languages, a fact that is reflected in the media and in the literature through their oral and written forms. These difficulties result in high rates of failures due to problems encountered in using the European standard by teachers and writers who prepare the school books. This research suggests the standardization of the Mozambican variant as well as the preparation of dictionaries and grammars illustrating the sociolinguistic reality of Mozambique in order to improve the quality of education. It also emphasizes the need for a self-esteem spirit on Mozambicans in general as a conduit to eliminate the soaring bias that Mozambicans can not speak portuguese language

Aparelho Herbst x osteotomia sagital mandibular bilateral no tratamento do padrão II, classe II, divisão 1 em adultos: relato de casos

Two treatment options are available for adult patients with skeletal Class II malocclusion caused by mandibular deficiency: combined mandibular advancement surgery and orthodontic treatment or mandibular advancement appliance. This study aimed to analyze the effects of two therapeutic modalities of Class II malocclusion treatment with mandibular deficiency. Two distinct individuals with Class II malocclusion division 1 and mandibular deficiency were treated after growth spurt. The first individual used the Herbst appliance as a therapeutic option and the second individual was treated with bilateral sagittal osteotomy. The cephalometric, occlusion and face results were evaluated for both individuals. Correction of Class II malocclusion was observed on both Herbst and surgery patients resulting on a normal occlusal relationship with normal overjet and overbite. Therefore it was concluded that Herbst appliance can be used to treat borderline skeletal Class II in adult patients.

Identidade e reconhecimento na cultura de rua

Direct descendant of the music of the Black Atlantic, hip hop became the spokesman of excluded minorities, for example setting up of the reunification that is geographically separated through communication. The hip hop appropriated new forms of mass communication, allowing the reworking of the story through a counter-hegemonic discourse that seeks self-knowledge and appreciation of the roots of black people. The street culture is the narrative of disenfranchised youth, generating narrative of inclusion, which gave a space of enunciation periphery enabling them to intersubjective recognition. This project aims to discuss the role of hip hop as an agent conscientizing youth peripherals in order to bring to the debate the form of identity construction around the peripheral street culture since, as an intervention that acts as a symbolic system guiding cultural practices and attitudes of these young people. Methodologically, we analyzed specific bibliographical about hip hop and literature originating from the periphery itself. Selected discography of some rap groups. They are Racionais Mc's, 509-E and DJ Hum and Thaíde that constitute the vanguard of hip hop in Brazil. We use music from rapper Emicida this while some contemporary exponents of rap, with national and international. State of São Paulo in order to demonstrate that there is similarity between the questions proposed by the hip hop and the interior of the capital, the group selected Survey. We have used also press material, raising the issue in research on the internet, periodicals include the Rolling Stones and Caros Amigos