2—酮基—L—古龙酸高产融合子15的形态学及生理生化特征的研究

由地衣芽孢杆菌（Ｂａｃｉｌｌｕｓｌｉｃｈｅｎｉｆｏｒｍｉｓ）Ｈ１９和２—酮基—Ｌ—古龙酸产生菌Ｓ１９的原生质体融合，得到了既能独立生长传代，又能产生２—酮基—Ｌ—古龙酸的１５号融合子。其菌落和菌体形态类似于Ｈ１９亲本，其生理生化特性也大多酷似Ｈ１９亲本而不同于Ｓ１９亲本。但其产生２—酮基—Ｌ—古龙酸的特性、乙醇氧化反应、石蕊牛奶产酸以及精氨酸双水解酶阴性等特点又酷似Ｓ１９亲本而不同于Ｈ１９亲本。其氧化酶反应、初始生长ｐＨ范围、苯丙氨酸和色氨酸脱氨酶反应则不同于双亲本。其菌体的氨基酸组份及含量也与双亲有一定差异。

Vc二步发酵新组合菌系B_(15-14)的筛选及其条件优化

筛选出了一组Vc二步发酵的新组合菌系(命名为B15-14),研究了环境因子对新菌系产酸的影响。结果显示:新组合菌系的最适底糖浓度为7%;温度为29~33℃,31℃为最适;适当增加通气量(20~30 mL装液量,250 mL三角瓶)有利于产酸;起始pH值范围为6.0~7.5;以B t菌做伴生菌时,发酵40 h就已达到终点,明显缩短了发酵时间。

北方农牧交错带耕地面积变化驱动力研究——以沽源县近15年为例

土地利用/土地覆被变化研究中,驱动力的研究是一个重要方面。从地理学的角度入手,将耕地面积变化的驱动因子归为降水量、经济、人口、技术、政策等方面,得到定量度量各驱动因子影响的多元线性模型,并用路径分析方法探讨驱动因子与耕地面积变化以及各驱动因子之间的关系。结论主要是:税费负担的加重会迫使农民开垦更多的耕地,取消农业税有利于生态建设;人口压力使得耕地面积扩大,不利于退耕还林草,因此要注意控制当地的人口数量;提高种植业人均纯收入水平,有利于控制开垦面积;在各驱动因子中,技术因素和经济因素对耕地面积变化最具影响。

Structure and acidity of Mo/H-MCM-22 catalysts studied by NMR spectroscopy

A comprehensive study on physical and chemical properties of Mo/MCM-22 bifunctional catalysts has been made by using combined analytic and spectroscopic techniques, such as adsorption, elemental analysis, and Xe-129 and P-31 NMR of adsorbed trialkylphosphine oxide probe molecules. Samples prepared by the impregnation method with Mo loadings ranging from 2-10 wt.% have been examined and the results are compared with that obtained from samples prepared by mechanical mixing using MoO3 or Mo2C as agents. Sample calcination treatment is essential in achieving a well-dispersed metal species in Mo/MCM-22. It was found that, upon initial incorporation, the Mo species tend to inactivate both Bronsted and Lewis sites locate predominantly in the supercages rather than the 10-membered ring channels of MCM-22. However, as the Mo loading exceeds 6 wt.%, the excessive Mo species tend to migrate toward extracrystalline surfaces of the catalyst. A consistent decrease in concentrations of acid sites with increasing Mo loading < 6 wt.% was found, especially for those with higher acid strengths. Upon loading of Mo > 6 wt.%, further decreases in both Bronsted and Lewis acidities were observed. These results provide crucial supports for interpreting the peculiar behaviors previously observed during the conversion of methane to benzene over Mo/MCM-22 catalyst under non-oxidative conditions, in which an optimal performance was achieved with a Mo loading of 6 wt.%. The effects of Mo incorporation on porosity and acidity features of the catalyst are discussed. (C) 2004 Published by Elsevier B.V.

凋落叶多样性对杉木幼苗生长及吸收~(15)N标记硫铵的影响

利用15N硫铵研究了凋落叶多样性对杉木幼苗生长及养分吸收的影响 .结果表明 ,凋落叶多样性的增加有利于盆栽杉木幼苗的生长 .杉木、火力楠、红栲和刺楸 4种凋落叶混合处理后 ,杉木幼苗的生长量最大 ;杉木、火力楠、刺楸 3种凋落叶混合处理后的杉木幼苗生长量次之 ,其它依次为杉木、火力楠、红栲 3种凋落叶混合处理 >杉木和刺楸凋落叶处理 >杉木和红栲凋落叶处理 >对照 >杉木和火力楠 2种凋落叶混合处理 >杉木凋落叶处理 .就杉木幼苗对硫铵氮的吸收率而言 ,不作任何处理的杉木幼苗吸收率最高 ,其次为杉木、火力楠、红栲和刺楸 4种凋落叶混合处理 ,其它依次为杉木、火力楠、刺楸 3种凋落叶混合处理和杉木、火力楠、红栲 3种凋落叶混合处理 >杉木和刺楸凋落叶处理 >杉木和红栲凋落叶处理>杉木和火力楠 2种凋落叶混合处理 >杉木凋落叶处理 .另外 ,用凋落叶处理后 ,土壤中硫铵氮的残留量比不作凋落叶处理的土壤多 ,硫铵氮的总回收量也比不作凋落叶处理的土壤大幅增加 ,而且凋落叶多样性的增加也会增加硫铵氮的残留量 .