Caracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos

The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1 M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1 M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.

Potencial de lixiviação de herbicidas utilizados na cana-de-açúcar em solos contrastantes

The increased use of herbicides, coupled with inadequate use of these molecules, also increased concerns about the risks of environmental contamination and its effects on humans and animals. The objective of this work evaluates the potential for leaching of herbicides in the crop of cane sugar, in contrasting soils. Used samples were used Psament (NR - sandy texture) and Oxisol Red (LR - clay texture). After application of herbicides ametryne (NR 1.60 kg ha(-1) and LR 2.40 kg ha(-1)), clomazone (NR 0.90 kg ha(-1) and LR 1.10 kg ha(-1)) and diuron (NR 1.60 kg ha(-1) and LR 3.20 kg ha(-1)), were irrigated blades of 0, 20, 40, 60, 80 and 100 mm of water in soil columns. Results indicate that ametryne in NR samples submitted to the handling layer of 5-10 cm when applied blades of 20 and 40 mm of water. It was evident that the clomazone, regardless of the soil, did not exceed 0-5 cm water depths of 0 to 20 mm of water and 5-10 cm deep in the blades above 40 mm of water. With blades up to 80 mm, the diuron leaching only in layers 0-5 cm deep in LR. Conclude that the potential of leaching of diuron and ametryne was influenced by soil texture together with the organic matter content, the same was not true for clomazone.

Greater expression of the human leukocyte antigen-G (HLA-G) and interleukin-17 (IL-17) in cervical intraepithelial neoplasia: analytical cross-sectional study

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo do extrato de glândulas salivares de fêmeas de carrapatos Rhipicephalus sanguineos sobre a morfologia de células tumorais da linhagem Walker 256

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Exometaboloma do tegumento da vespa social Polybia paulista por análises GC-MS: bases moleculares do reconhecimento de tipos parentais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Controle de fotoassociação caótica com campos externos

The objective of the present work was to study the control of the dynamics of diatomic heteronuclear molecules interacting with electric fields created by lasers. Specifically in this work, the molecular photoassociation phenomenon will be analyzed. At this phenomenon, the atom's relative movement is described by a particle that moves in a morse potential well under the influence of an external time dependant force related to the external field. Based on the optimum control theory (OCT), it is presented at the present work laser pulses that alternate a given initial molecular state to a desirable end state, wich in this work was represented by the minimization of a cost functional that indicates how close. To do so, a computational sistem know as Genetic Algorithm (GA) was developed that can be characterizes as an extremelly eficient technique capable of scanning the solutions space and find results close to the optimum solutions

Apoptose no carcinoma de próstata

Pós-graduação em Patologia - FMB

Aspectos técnicos, econômicos e de sustentabilidade da produção de hidrogênio renovável

Pós-graduação em Engenharia Mecânica - FEG

Determinação de sólidos solúveis e firmeza em frutos íntegros de pessegueiro por meio da espectroscopia do infravermelho próximo (NIR)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da doxicilina na hipertensão arterial induzida pela exposiçao ao chumbo: quantificação de pepídeos vasoativos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Migração e invasão celular in vitro de células humanas expressando variantes da oncoproteína LMP1 do vírus de Epstein-Barr (EBV)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Transcrição cooperativa de genes ribossomais em Escherichia coli usando um modelo estocástico e dependente de sequência

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo dos mecanismos de resistência e virulência de isolados de Cryptococcus neoformans e Cryptococcus gattii e quantificação de genes de bomba de efluxo pós tratamento com Galatos de Alquila

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Funcionalidade das proteínas EF-Tu e 14-3-3 na virulência de Paracoccidioides brasiliensis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Exploração racional da rede metabólica de Xylaria sp. visando à produção de metabólitos de interesse farmacológico através de ferramentas quimiométricas e técnicas de desreplicação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)