Sporothrix schenckii sensu stricto Isolated from Soil in an Armadillo's Burrow

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Decay of interspecific avian flock networks along a disturbance gradient in Amazonia

Our understanding of how anthropogenic habitat change shapes species interactions is in its infancy. This is in large part because analytical approaches such as network theory have only recently been applied to characterize complex community dynamics. Network models are a powerful tool for quantifying how ecological interactions are affected by habitat modification because they provide metrics that quantify community structure and function. Here, we examine how large-scale habitat alteration has affected ecological interactions among mixed-species flocking birds in Amazonian rainforest. These flocks provide a model system for investigating how habitat heterogeneity influences non-trophic interactions and the subsequent social structure of forest-dependent mixed-species bird flocks. We analyse 21 flock interaction networks throughout a mosaic of primary forest, fragments of varying sizes and secondary forest (SF) at the Biological Dynamics of Forest Fragments Project in central Amazonian Brazil. Habitat type had a strong effect on network structure at the levels of both species and flock. Frequency of associations among species, as summarized by weighted degree, declined with increasing levels of forest fragmentation and SF. At the flock level, clustering coefficients and overall attendance positively correlated with mean vegetation height, indicating a strong effect of habitat structure on flock cohesion and stability. Prior research has shown that trophic interactions are often resilient to large-scale changes in habitat structure because species are ecologically redundant. By contrast, our results suggest that behavioural interactions and the structure of non-trophic networks are highly sensitive to environmental change. Thus, a more nuanced, system-by-system approach may be needed when thinking about the resiliency of ecological networks.

Morphological traits and type of dairy goats registered in Brazil from 1976 to 2009

Data from 2439 goats of the Saanen, Alpine, Anglo Nubian and Toggenburg breeds recorded from 1976 to 2009 by the Association of Goats and Sheep Breeders of Minas Gerais were used in principal component analysis. After consistency of data, six morphological variables (thorax perimeter, body length, withers height, height, width and length of the rump) and 12 variables related to breed standard score and fitness (breed characteristic, head, palette and topline, feet and legs, dairy type, body capacity, udder, rear and front ligament, udder texture, teat and final score) were analyzed. Based on the magnitude of the eigenvalue (lower than 0.7), eleven variables considered redundant were discarded, resulting in reduced costs of technician labor to evaluate the animals. Maintenance of records on height, length, rump width, breed characteristic, dairy type, front ligament and udder texture is recommended.

Polydomy in the ant Ectatomma opaciventre

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ocorrência de Escherichia coli O157:H7 e O26 sorbitol negativas em matadouro frigorífico de bovino e suscetibilidade a antimicrobianos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Transgressões de grafia em produções textuais de escolares do programa educacional de resistência às drogas e à violência (PROERD)

Presence of the orthographic mistakes on the writting of the students with or without learning difficulties still is a concern. The focus of these study is the classification of orthographic mistakes on the writting of the students who had participated of the Educational Program of Resistance to the Drugs and the Violence (Proerd). Sixty four grade children from a public school were individually analyzed in this study. The aim of this study was to verify the frequency and characterization of the orthographic mistakes on the texts production these students. We had as base for this analysis the studies of Zorzi (1998). The most frequent mistakes made by students were: irregular phonographemic relation (24%), letters omission (18%), similar letters (13%) and hypo-segmentation and hyper-segmentation (11,0%), amongst others. The characterization of the mistakes writing makes possible the planning of the interventions, as well as, the use of the prevention strategies.

Avaliação de Técnicas de Interpolação de Imagens Digitais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O Método de Kane aplicado a um modelo de corpo humano para obtenção da pressão intradiscal na coluna lombar

Nos últimos anos tem surgido vários métodos para se estudar sistemas multicorpos. A vantagem destes métodos é a obtenção das equações do movimento de forma mais fácil e precisa. Robôs, manipuladores, mecanismos, satélites, máquinas em geral e sistemas biomcânicos, pelas suas características, podem ser vantajosamente tratados por formalismo multicorpo, mas precisamente, o método de Kane a um modelo do corpo humano, com a finalidade de se determinar a pressão intradiscal entre as vértebras L4/L5 da coluna lombar, em algumas atividades normais de vida diária, como: sentar, abaixar, pegar e elevar cargas, realizar movimentos de flexão e extensão. Pretendeu-se, também obter os esforços internos nas diversas articulações deste modelo. A pressão intradiscal representa um parâmetro importante para caracterizar a sobrecarga na coluna, podendo ter relação com a degeneração do disco intervertebral, o que produz muita dor em indivíduos. Inicialmente, o modelo foi proposto e o método de Kane foi aplicado para se obter as equações do movimento. Para resolvê-las, foi necessário realiza a filmagem de um sujeito realizando as atividades citadas, a fim de se obter as equações das trajetórias dos diversos segmentos do corpo humano. Após a simulação, os parâmetros desejados foram obtidos e comparados com valores experimentais citados na literatura.

Building the sugarcane genome for biotechnology and identifying evolutionary trends

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diagnóstico molecular da alteração mutagênica nt 230 (del4) no gene MDR1 em cães

P-glycoprotein is an adenosine triphosphate (ATP)-driven drug efflux carrier responsible for transport of xenobiotics and multiple classes of drugs, many usually use in veterinary medicine. Encoded by MDR1 gene, also referred to as ABCB1, located on chromosome 14, is expressed in many tissues with secretory or excretory functions, such as liver, kidney and intestine, where it limits drug absorption from the gut and promotes drug excretion into the bile and urine of their substrates. In 2001, a 4 base pair gene deletion mutation in the canine MDR1 gene was identified as MDR1-1▲, ABCB1-1▲, MDR1 MDR1 nt 230 (del4) and associated with an non-functional Pglycoprotein. The clinical correlation is the (hyper) sensitivity of certain dogs breeds, mostly collies, to a few classes of drugs such as anticancer drugs (doxorubicin, vincristine, vinblastine), immunosuppressants (cyclosporine), antiparasitic drugs (ivermectin, moxidectin), steroids hormones (aldosterone, cortisol, dexamethasone), antimicrobial agents (tetracycline, doxycycline, levofloxacin, ketoconazole, itraconazole), analgesics (morphine, methadone), antidiarrheals (loperamide), antiepileptic agents (phenothiazine), cardiac drugs (digoxin, diltiazem, verapamil, talinolol) and others. Dogs with homozygous MDR1 nt 230 (del4) MDR1 mutations (MDR1 - / -) have a higher predisposition to intoxication with substrates of P-gp than heterozygous (MDR1 + / -) and these are more likely than dogs homozygous nonmutant (MDR1 +/ +). After the identification of nt230 (del4) mutation, several molecular techniques have been developed for identification of mutant animals as a diagnostic method. The importance of molecular diagnosis is, after the identification of mutant animals, establish treatment protocols safe, exclude this animals from reproduction (genetic selection program) and investigating the history of adverse drugs reactions... (Complete abstract click electronic access below)

Staphylococcus sp na produção primária de leite: aspectos de saúde pública

Staphylococcus is one of the more important causes of the called Foodborne Disease(FD), being that from the 40 species described from genus, the more important is Staphylococcus aureus. During years believed that the S. aureus was the only specie from genus able to produce enterotoxins, responsable for the clinical frame in humans, but latest studies report the isolation of other species both positive coagulase (PC) as negative with enterotoxigenic potential. The symptoms of this intoxication appear after a short period of incubation (2-6 hours) and usually characterized by nausea, vomits, abdominal ache, diarrhea, and rarely is fatal. For the toxin to be formed in food is necessary that bacteria population to be at least 105 UFC/g, being that such toxins characterized by presenting great resistance front of gastrointestinal proteases and of homemade termical treatment. Among the main foods that might carry the microorganism, the milk and its derivatives have highlights. The contamination of the product might happen as from the milk from cows with clinical and/or subclinical mastitis, as the Staphylococcus genus is one of the main agents etiologic from this disease, equipments utensils badly sanitized equipments and utensils and from the manipulators. The control of these factors configures as fundamental condition for the achievement of a safe, quality product, which doesn’t offer risk to the consumers

Análise Prosódica de segmentações não-convecionais de palavras em textos do sexto ano do EF

This paper analyzes unconventional segmentation of word found in texts of the sixth grade of Elementary School. Through quantitative and qualitative analysis, we describe the prosodic characteristics that may be motivated the hyper and hippossegmentation of words found in 27,4% of 606 investigated texts. We identified a tendency toward a more text with hippossegmentation than hypersegmentation, characteristic similar to what is reported about children’s text. Taking into account the theoretical framework of Prosodic Phonology, we argue about the relevance of prosodic word and clitic group in the description of the regularities observed in data of unconventional segmentation of word. We note that (i) in cases of hipposegmentation, it predominates the hollow of a clitic followed by a phonological word, (ii) in cases of hypersegmentation, it predominates a segmentation of a prosodic word into a clitic group. We present evidences to be the spelling of clitic elements a challenge to students analyzed. By investigating in the grammatical class that owned the clitics spelled unconventionally, we verified that they are prepositions (“em, de, com”) and pronouns (“me, lhe, lo”), a characteristic that particularize these data in relation to data from students in the early stage of language acquisition.

Práticas de leitura e escrita em contexto acadêmico: relações (hiper)textuais singulares

The present article aims at reflecting about the discursive practices of writing and reading on the net, more specifically about the writing and reading methods used by the undergraduate and graduated teachers, based on an internet search engine. It’s of interest to investigate: i) the (hyper) textual relations established in the context thought as permitted by the electronic resources; ii) the discursive marks that arise (are arisen) in a singular way of reading (and/or writing). The set of material was produced during a university extension course about reading and cyberspace, whose context consisted of a drawing production of the reading process on an internet search engine, on the basis of the signifier “apple”. Based on the French Discourse Analysis and assumptions from the New Literacy Studies, we intended to discuss the operating procedures of the internet search and the effects of meanings produced by the subject during his/her reading/writing process.

Partial purification, immobilization and preliminary biochemical characterization of lipases from Rhizomucor pusillus

Lipases have important applications in biotechnological processes, motivating us to produce, purify, immobilize and perform a biochemical characterization of the lipase from Rhizomucor pusillus. The fungus was cultivated by solid state fermentation producing lipolytic activity of about 0.5 U/mL(4U/g). A partial purification by gel filtration chromatography in Se-phacryl S-100 allowed obtaining a yield of about 85% and a purification factor of 5.7. Our results revealed that the purified enzyme is very stable with some significant differences in its properties when compared to crude extract. The crude enzyme extract has an optimum pH and temperature of 7.5 ° C and 40 ° C, respectively. After purification, a shift of the optimum pH from 7 to 8 was observed, as well as a rise in optimumtemperature to 60 ° C and an increase in stability. The enzyme was immobilized on CNBr-Agarose and Octyl-Agarose supports, having the highest immobilization yield of 94% in the second resin. The major advantage of immobilization in hydrophobic media such as Octyl is in its hyper activation, which in this case was over 200%, a very interesting finding. Another advantage of this type of immobilization is the possibility of using the derivatives in biotechnological applications, such as in oil enriched with omega-3 as the results obtained in this study display the hydrolysis of 40% EPA and 7% DHA from sardine oil, promising results compared to the literature.

A narrativa audiovisual da transmissão direta e ao vivo da copa do mundo da FIFA: comparação entre a televisão analógica e a digital

Pós-graduação em Comunicação - FAAC