Determinação de genes mórbidos e drogáveis a partir da construção e análise da rede integrada de interações moleculares entre genes humanos

The discovering process of new morbid genes and new target proteins for drugs have been shown to be very costly and laborious. Having in view cutting costs and speeding up this process, we propose, in this work, a new method to determine the gene druggability score and morbidity score, the probabilities of the protein encoded by the gene have the characteristics that make it a new target for drugs and in case of an alteration in that gene, we observed a phenotype that characterizes a genetic based illness. To determine these characteristics, we built, analyzed and determined the characteristics of the topology of the integrated molecular interactions network among human genes containing physical interactions between proteins, metabolic interactions and interactions of transcriptional regulation, and included other data such as level of gene transcription and cellular localization of the protein encoded by the gene. We tested our model in training sets and achieved results equal or better than the ones achieved by similar methods in the literature. Finally, with the purpose of investigating whether the assigned scores resembles the potential druggabilities and morbities of the previously unclassi ed genes, we looked for evidences in biomedical literature supporting the potential druggability and morbidity status of genes with the 10 highest scores. We found clear evidences for 73% and 90% of potential druggable and morbid genes respectively

Concentrações de nitrogênio e potássio na solução nutritiva do pimentão cultivado em substrato sem drenagem

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Resposta da soqueira da cana-de-açúcar à aplicação de nitrogênio na ausência e na presença de silício

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Caracterização biométrica e germinação de mabolo (Diospyros blancoi Willd)

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Amostragem diurna versus noturna de ácaros (Arachnida: Acari) associados a Genipa americana L. (Rubiaceae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação de biofertilizante, composto e uréia na produção de capim tanzânia (Panicum maximum, Jacq.) sob irrigação

Pós-graduação em Zootecnia - FCAV

Doses de potássio e umidades de solo para o crescimento de plantas de mogno-africano

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Influência de diferentes métodos de limpeza sobre a eficiência de corte e as características morfológicas de fresas Carbide

Introduction: Currently, there are many questions regarding the cleaning methods seeking greater efficiency and less loss of burs. Aim: the aim of this study was to evaluate the influence of cleaning methods on the cutting efficiency and morphological characteristics of stainless steel burs tungsten carbide (carbide). Materials and method: Thirty burs were divided into five groups (n = 5) according with the cleaning method: L1 - steel brush, L2 - nylon brush, L3 - ultrasound  +  distilled water, L4 - ultrasound + descaling solution and L5 - no cleaning method (control). The burs were used for the cutting of bovine enamel during six periods of 12 minutes each. After each period, the burs were cleaned (except L5 ) following the protocol established for each group. The cutting efficiency was determined by mass loss and morphological characteristics. Result: The average amount of wear after 72 minutes of use were L1  = 0.3558 g; L2  = 0.4275 g; L3  = 0.4652 g; L4  = 0.4396 g e L5  = 0.4854 g; significant differences in the time of use (p  <  0.001) and cleaning method (p  <  0.001). The L1 group showed the worst performance. Regardless of the experimental group, morphological analysis revealed alterations in the cutting blades soon after the first 12 minutes, being L1 the most affected group. Conclusion: The cleaning with wire brush was the most damaging method to the cutting efficiency and to the morphology of carbide burs.

Deformación del área de corte de las limas endodónticas sometidas a diferentes métodos de precurvatura

Objetive: Evaluate the deformations caused in the cutting edges in the K-file #25 (LK), subjected to pre-beinding of the apcial third using the following methods: finder-bending, cotton-pliers bendin and Flexobend, observed under scanning electron microscopy (SEM). Material and methods: Forty-eight LK#25 were divides into 4 groups (n=12); G1, control; G2, finger-bending; G3, cotton-plier bending, G4, Flexobend. The curvature was standardized in the apical segment of the file at 2 mm of D!. Was constructed a model of acrylic resin that correspond to a canal of 20º of curvature. The files were examined under SEM (260X). Data were analyzed using Fisher´s statistical test with a significance level of 5%. Results: The images showed no alterations in the LK, similar to G3, with no statistical difference. G3 and G4 were statistically different form the other groups (p<0.05). Conclusions: the finger-bending method to curve apical third of LK #25 did not alter the cutting edges, the cotton-pliers bending and Flexobend did alter the cutting edges of the files (AU)

Doses de fósforo na produção das mudas e sua influência na produção e qualidade de tomate

Pós-graduação em Agronomia (Horticultura) - FCA

Análise comparativa dos métodos MQL, MQL/Água e convencional na retificação cilíndrica do aço ABNT 52100, com rebolo de óxido de alumínio

Pós-graduação em Engenharia Mecânica - FEB

Respostas bioquímicas e fisiológicas de plantas jovens de cana-de-açúcar sob diferentes concentrações de NaCl no solo

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Expression Pattern and Promoter Analysis of a Eucalyptus grandis Germin-like Gene

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Determinação dos tipos de resistência a Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) em genótipos de soja

The aim of this work was to evaluate the resistance types in soybean genotypes to Spodoptera cosmioides (Walker) in laboratory. Soybean genotypes assessed were as follows: 'IAC 100'(resistance standard), 'BR16'(susceptible standard), 'Dowling', PI 227687, PI 274454, 'IGRA RA 626 RR', PI 227682, 'BRSGO 8360', 'IGRA RA 516 RR'and 'P 98Y11 RR'. Free-choice and no-choice feeding non-preference tests were done using two newly-hatched larvae per genotype or one third-instar larva per genotype in both tests. Larvae attractiveness was evaluated in different times, and at the end of the experiments the leaf area consumed was quantified. In the antibiosis test, newly-hatched larvae were individualized into Petri dishes, where leaflets of the genotypes were offered over the larval stage, and the following biological parameters were assessed: period and viability of larvae, pupae and overall (larvae + pupae), weight of larvae and pupae, sex ratio and adults longevity. Overall, in the feeding preference tests, significant differences were not found in leaf consumption among the genotypes. In the antibiosis assay, genotypes PI 227687, PI 227682 and 'IAC 100'caused 100% larval mortality and the lowest weight of larvae, ranging between 37.65 and 85.56 mg. All soybean genotypes evaluated do not exhibit feeding non-preference type resistance to S. cosmioides, and PI 227687, PI 227682 and 'IAC 100'highlighted for possessing antibiosis.

Características agronômicas, bromatológicas e econômicas de alturas de corte para ensilagem da cultura do milho

The aim was to evaluate agronomic, bromatologic and economical characteristics harvest heights for ensiling of corn. The trial was conducted in randomized block design and treatments corresponded to harvest heights of 20; 50; 80 and 110 cm from the soil surface. The agronomic evaluations were conducted 114 days after planting. The percentage of dry matter (DM) and productivity of stem, grain and participation of potassium remaining in the stem increased linearly with increasing cutting height (p<0.05). Therefore, the mass used at ensiling decreased proportionally with increasing cutting height (p<0.05). The silage quality was improved because of the increase in the cutting height. We observed higher crude protein (CP) content and energetic value in the silage harvested at 110 cm from the soil surface, while there was lower fiber content. However, silages produced with plants harvested at 110 cm from the soil surface showed the highest production cost (4.47 times greater than cycling through K in the remainder of the stem). The agronomic and bromatologic characteristics of corn silage improves with increasing cutting height, but the increase cutting height unfeasible economically the practice silage when the generation of final products is not computed. Thus, it is recommended that the cutting height of the corn plant is at most 50 cm.