Detecção de herpesvírus bovino 5 (BoHV-5) em bovinos do Sudeste Brasileiro

This paper reports the detection of bovine herpesvirus type 5 (BoHV-5) by a specific nested PCR assay. Samples were collected from the central nervous system (CNS) of cattle from Minas Gerais and São Paulo States, Brazil. All animals died presenting neurological symptoms. Nineteen frozen CNS samples analyzed had been previously tested by fluorescence antibody test for rabies virus and showed negative results. Three paraffin-embedded brain tissue samples were examined by histopatology and the observed alterations suggested nonsuppurative meningoencephalitis. BoHV-5 was detected in five (22.7%) among 22 tested samples. The occurrence of BoHV-5 infection is reported in the Southeast region of Brazil, indicating that epidemiological studies should be carried out.

Avaliação do óleo de soja submetido ao processo de fritura de alimentos diversos

The main objective of this study was to evaluate the degree of degradation of the soybean oil used in frying processes. The quality of the oil during the frying process was evaluated by means of physical-chemical analyses, such: conjugated dienes (%) and TBA index (mmoles g(-1)), and determination of total polar compounds (%). It was observed that the values of conjugated dienes increasing significantly with time until 1.87% after 15 hours for fry pans I and 1.76% after 22.5 hours for fry pans II. The TBA indexes reached at the end the frying process values of 0.0265 and 0.0210 mmoles g(-1) for fry pans I and II, respectively. The percentages of total polar compounds have shown an increase of six and eight times in relation to the original sample starting from 5.9% and 3.7% and reaching values of 36.8% and 29.2%, for fry pan I and II, after 15 and 22.5 hours of heating, respectively. It has been concluded that the alterations of the soybean oil increased as the time of frying increased, for both fry pans. The oil used in fry pan I showed greater alteration than the that observed in the oil used in fry pan II, in spite of the time of heating being shorter. Taking into consideration that the levels of polar compounds should be less than 25% according to the legislation of several countries, the oils used in both fry pans were already inadequate conditions at the moment of disposal.

Energia e nutrientes digestíveis de alimentos para a tilápia do nilo

The utilization of energetic and protein feeds, highly digestible as well, becomes more and more necessary due to the quality of formulated rations, fish performance and relationship with the environment. Apparent digestibility coefficient (ADC) of dry matter, protein, energy, phosphorus, and amino acids of corn starch, corn, wheat, rice, soybean, and cottonseed meal, corn gluten and fish meal were determined for Nile tilapia. ADC was determined using a reference diet based on albumin, gelatin and corn starch, was used inert indicator chromium III oxide (Cr(2)O(3)). Each test diet composed by 70% of reference diet and 30% of the test diet. Feces were collected using mofied Guelph system. ADC values for protein and average ADC of amino acids were as follows: corn 89.76 and 96.43%, rice meal 95.88 and 92.26%, wheat meal 93.54 and 84.41%, fish meal 82.59 and 86.36%, corn gluten 89.82 and 87.98%, soybean meal 94.13 and 91.93%, cotton meal 87.10 and 77.47%, respectively. According to the results of this work, ADC of protein is not a reliable indicator of ADC values of amino acids, even more so for wheat meal, corn, and cotton meal. Among protein feeds, soybean meal was found to have the highest ADC for protein and amino acids, while corn was the energetic feed with the highest ADC (86.15%) for energy.

Investigação de parasitoses e/ou comensais intestinais em manipuladores de alimentos de escolas públicas

The objective of this work was to identify intestinal parasites and/or commensals among food handlers of public schools in the city of Patos of Minas (MG). The fecal samples were analyzed by Lutz's method (1919). The positive results were 21.7% (13 cases) for parasites and/or commensal. The frequence of intestinal parasites and/or commensal of food handlers in the public schools showed low positive, probably as a result of good habits of personal hygiene and prophylactic treatment of parasitic.

Mudança de concepção das políticas públicas para o campo brasileiro: o Programa de Aquisição de Alimentos, PAA

In the 90, the public politics for the Brazilian rural way incorporated the territorial perspective, besides they motivate the participation and the beneficiaries collective organization. In 2003 the Food Acquisition Program (FAP) was created with the intention of guaranteeing the feeding of people in social vulnerability situation or alimentary insecurity and of providing work and income in the field through the direct buying of the agriculturists foods. In a research made in two São Paulo State's towns: Tupi Paulista and Dracena, it was verified that the price paid to the farmers is higher to the regional average and because of this fact the participants have interest in enlarging the cultivated area and/or in diversifying the cultivations. Though, two problems were detected: 1) the reduced number of beneficiated producers; and, ii) the furtherance of the producers relatives already registered that try to increase their quota of products and the value to be received.

Herpes Virus Bovino tipo 1 (BoHV-1) como posible causa de encefalitis en bovinos de la región del Magdalena Medio Colombiano. Estudio serológico y análisis epidemiológico

The bovine Herpesvirus type 1 and type 5 (BoHV-1 and BoHV-5), causing diseases and significant economic losses in farms of worldwide. Both affect the nervous system of cattle, although BoHV-5 has been the most associated with this type of pathogenesis. Given the death of animals with nervous symptoms and negative diagnoses for rabies virus in the area of study, this research focused on the detection of positive reactors to bovine herpes virus serum neutralization. We collected 518 blood samples from animals without Herpesvirus vaccine, in the municipalities of Caparrapi, Cimitarra, Honda and Victoria, in the Middle Magdalena River Region. In addition, epidemiological information useful to discuss neurological disease was collected through primary and secondary sources. For the analysis of data was used chi-square test by identification of relationship between evidence of viral infection and the variables recorded. The results revealed that 286 cases were positive for Herpesvirus infection, corresponding to a prevalence of 55.5%, however, there was no statistical relationship (p < 0.05) between the presence of antibodies and the variables analyzed. In conclusion, some cases of neurological disease in cattle in this region could be due to infection with herpes viruses. We discussed about the presence of BoHV-1 and BoHV-5 in the ambient, diagnosis and monitoring plans, as well as economic losses, which may cause in herds in this area.

Prevalência de reagentes ao teste de tuberculinização no rebanho bovino leiteiro da região de São Carlos - SP

Tuberculosis (TB), an infectious disease readily transmitted between animals and humans, is an great threat to public and animal health a like and has been declared an international emergency. According to the WHO, animal TB is a source of much concern, especially in the poorer countries where there is little awareness of the problem. Thus, improvements in veterinary health are essential, with regard to infection by Mycobacterium bovis, particularly in areas where the public is at risk. The prevalence of infected animals in Brazil is about 1%, whereas, in the dairy cattle region of São Carlos, the average proportion of positive tests was found to be 3. 1% from 1987 to 1996. The current research was carried out to investigate the prevalence, in the region, of dairy cattle reacting positively to the tuberculin test, from 1997 to the first half of 2001. The results were obtained by searching through the data collected by the Federal Inspection Authority, and they indicated an average prevalence of 1.3% over the period, similar to the official national average. It was concluded that bovine TB continues to be present in the region, posing a potential threat of infection to the human population.

Peso de abate de machos não-castrados para produção do bovino jovem. 1. Desempenho em confinamento e custos de produção

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Peso de abate de machos não-castrados para produção do bovino jovem. 2. Peso, Idade e caracteristicas da carcaça

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Métodos de coleta de fezes e determinação dos coeficientes de digestibilidade da fração protéica e da energia de alimentos para o pacu, Piaractus mesopotamicus (Holmberg, 1887)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção de Leptospira pomona em sêmen bovino por eletroforese capilar fluorescente

This study was performed in order to evaluate the detection limit of PCR with fluorescent capillary electrophoresis for Leptospira pomona diagnosis in bovine semen. Negative bovine semen samples were artificially contaminated with Leptospira pomona (10 0 to 10 7 bacteria/ ml) and DNA was extracted by phenol/chloroform protocol. DNA fragments visualization was done by three electrophoresis methods: under UV light in 2 % agarose gel, silver staining 8% polyacrylamide gel and fluorescent capillary electrophoresis. The detection limit of capillary electrophoresis for Leptospira pomona was 10 2bacteria/ml. Under UV light, in 2 % agarose gel, the detection limit was of 10 4 bacteria/ ml while for silver stained 8 % polyacrylamide gel it was 10 2 bacteria/ ml. PCR with fluorescent capillary electrophoresis is an efficient and rapid diagnostic test for DNA detection of Leptospira in bovine semen and this can be an important tool for herd and semen sanitary control in artificial insemination centers.