Distribution and co-occurrence of rockfishes (family: Sebastidae) over trawlable shelf and slope habitats of California and southern Oregon

The rockfishes of the sebastid genus Sebastes are a very important fishery resource off the coasts of California and southern Oregon. How-ever, many of the 54 managed stocks of west coast rockfish have recently reached historically low population levels, leading fishery managers to re-examine current management practices. Management of rockfish stocks as multispecies aggregates, as opposed to independent stocks within the ground-fish fishery, can be more desirable when nontargeted bycatch, discard, and management complexity are considered. Rockfish assemblage structure and species co-occurrences were determined by using data from the Alaska Fisheries Science Center triennial continental shelf bottom trawl survey. The weight of rockfish species in trawl catches was expressed as a catch-per-unit-of-effort (CPUE) statistic, from which species spatial distributions, overlaps, diversity, and richness were analyzed. Multidimensional scaling of transformed CPUE data was employed in indirect gradient and multivariate partitioning analyses to quantify assemblage relationships. Results indicated that rockfish distributions closely match the bathymetry of coastal waters. Indirect gradient analysis suggested that depth and latitude are the principal factors in structuring the spatial distributions of rockfish on trawlable habitat. In addition, four assemblages were identified through the joint evaluation of species’ distributions and multivariate partitioning analyses: 1) deep-water slope; 2) northern shelf; 3) southern shelf; and 4) nearshore. The slope, shelf, and near-shore groups are found in depth ranges of 200–500 m, 100–250 m, and 50–150 m, respectively. The division of northern and southern shelf assemblages occurs over a broad area between Cape Mendocino and Monterey Canyon. The results of this analysis are likely to have direct application in the management of rockfish stocks off the coasts of southern Oregon and California.

Co-expression of CD173 (H2) and CD174 (Lewis Y) with CD44 suggests that fucosylated histo-blood group antigens are markers of breast cancer-initiating cells

Histo-blood group antigens CD173 (H2) and CD174 (Lewis Y) are known to be developmentally regulated carbohydrate antigens which are expressed to a varying degree on many human carcinomas. We hypothesized that they might represent markers of cancer-initiating cells (or cancer stem cells, CSC). In order to test this hypothesis, we examined the co-expression of CD173 and CD174 with stem cell markers CD44 and CD133 by flow cytometry analysis, immunocytochemistry, and immunohistochemistry on cell lines and tissue sections from breast cancer. In three breast cancer cell lines, the percentage of CD173(+)/CD44(+) cells ranged from 17% to > 60% and of CD174(+)/CD44(+) from 21% to 57%. In breast cancer tissue sections from 15 patients, up to 50% of tumor cells simultaneously expressed CD173, CD174, and CD44 antigens. Co-expression of CD173 and CD174 with CD133 was also observed, but to a lesser percentage. Co-immunoprecipitation and sandwich ELISA experiments on breast cancer cell lines suggested that CD173 and CD174 are carried on the CD44 molecule. The results show that in these tissues CD173 (H2) and CD174 (LeY) are associated with CD44 expression, suggesting that these carbohydrate antigens are markers of cancer-initiating cells or of early progenitors of breast carcinomas.

南极隐兆线粒体CO II基因序列及无翅类昆虫分子进化

测定了采自南极隐兆（Cryptopygus nanjiensis）的线粒体细胞色素氧化酶亚基II（CO II）基因序列，并测定了拉提疣兆（Neanura latior）、梅坞格蚖（Gracilentulus maijiawensis）和韦氏鳞八（Lepidocampa weberi）的CO II基因序列，对序列的A + T含量、核苷酸取代和转换/颠换（TS/TV）频率进行了统计分析。计算了种间Tamura-Nei遗传距离，以甲壳类无甲目（Anostraca）的一种卤虫Artemia franciscana作为外群构建了分子系统树。对无翅类昆虫线粒体CO II基因序列A + T含量的进化倾向性、类群间的遗传分歧和系统进化关系进行了探讨。

Global linear and nonlinear stability of viscous confined plane wakes with co-flow

The global stability of confined uniform density wakes is studied numerically, using two-dimensional linear global modes and nonlinear direct numerical simulations. The wake inflow velocity is varied between different amounts of co-flow (base bleed). In accordance with previous studies, we find that the frequencies of both the most unstable linear and the saturated nonlinear global mode increase with confinement. For wake Reynolds number Re = 100 we find the confinement to be stabilising, decreasing the growth rate of the linear and the saturation amplitude of the nonlinear modes. The dampening effect is connected to the streamwise development of the base flow, and decreases for more parallel flows at higher Re. The linear analysis reveals that the critical wake velocities are almost identical for unconfined and confined wakes at Re ≈ 400. Further, the results are compared with literature data for an inviscid parallel wake. The confined wake is found to be more stable than its inviscid counterpart, whereas the unconfined wake is more unstable than the inviscid wake. The main reason for both is the base flow development. A detailed comparison of the linear and nonlinear results reveals that the most unstable linear global mode gives in all cases an excellent prediction of the initial nonlinear behaviour and therefore the stability boundary. However, the nonlinear saturated state is different, mainly for higher Re. For Re = 100, the saturated frequency differs less than 5% from the linear frequency, and trends regarding confinement observed in the linear analysis are confirmed.

Measurement-Integrated simulations and Kalman filter applied to a co-flowing jet

This paper deals with the experimental evaluation of a flow analysis system based on the integration between an under-resolved Navier-Stokes simulation and experimental measurements with the mechanism of feedback (referred to as Measurement-Integrated simulation), applied to the case of a planar turbulent co-flowing jet. The experiments are performed with inner-to-outer-jet velocity ratio around 2 and the Reynolds number based on the inner-jet heights about 10000. The measurement system is a high-speed PIV, which provides time-resolved data of the flow-field, on a field of view which extends to 20 jet heights downstream the jet outlet. The experimental data can thus be used both for providing the feedback data for the simulations and for validation of the MI-simulations over a wide region. The effect of reduced data-rate and spatial extent of the feedback (i.e. measurements are not available at each simulation time-step or discretization point) was investigated. At first simulations were run with full information in order to obtain an upper limit of the MI-simulations performance. The results show the potential of this methodology of reproducing first and second order statistics of the turbulent flow with good accuracy. Then, to deal with the reduced data different feedback strategies were tested. It was found that for small data-rate reduction the results are basically equivalent to the case of full-information feedback but as the feedback data-rate is reduced further the error increases and tend to be localized in regions of high turbulent activity. Moreover, it is found that the spatial distribution of the error looks qualitatively different for different feedback strategies. Feedback gain distributions calculated by optimal control theory are presented and proposed as a mean to make it possible to perform MI-simulations based on localized measurements only. So far, we have not been able to low error between measurements and simulations by using these gain distributions.