Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells

Mouse has become an increasingly important organism for modeling human diseases and for determining gene function in a mammalian context. Unfortunately, transposon-tagged mutagenesis, one of the most valuable tools for functional genomics, still is not available in this organism. On the other hand, it has long been speculated that members of the Tc1/mariner-like elements may be less dependent on host factors and, hence, can be introduced into heterologous organisms. However, this prediction has not been realized in mice. We report here the chromosomal transposition of the Sleeping Beauty (SB) element in mouse embryonic stem cells, providing evidence that it can be used as an in vivo mutagen in mice.

Regulated transposition of a fish transposon in the mouse germ line

Tc1/mariner elements are able to transpose in species other than the host from which they were isolated. As potential vectors for insertional mutagenesis and transgenesis of the mouse, these cut-and-paste transposons were tested for their ability to transpose in the mouse germ line. First, the levels of activity of several Tc1/mariner elements in mammalian cells were compared; the reconstructed fish transposon Sleeping Beauty (SB) was found to be an order of magnitude more efficient than the other tested transposons. SB then was introduced into the mouse germ line as a two-component system: one transgene for the expression of the transposase in the male germ line and a second transgene carrying a modified transposon. In 20% of the progeny of double transgenic male mice the transposon had jumped from the original chromosomal position into another locus. Analysis of the integration sites shows that these jumps indeed occurred through the action of SB transposase, and that SB has a strong preference for intrachromosomal transposition. Analysis of the excision sites suggests that double-strand breaks in haploid spermatids are repaired via nonhomologous end joining. The SB system may be a powerful tool for transposon mutagenesis of the mouse germ line.

Communicating strategically in the face of terrorism: The Spanish government’s response to the 2004 Madrid bombing attacks

With the re-emergence of insurgency tied to terrorism, governments need to strategically manage their communications. This paper analyzes the effect of the Spanish government’s messaging in the face of the Madrid bombing of March 11, 2004: unlike what happened with the 9/11 bombings in the USA and the 7/07 London attacks, the Spanish media did not support the government’s framing of the events. Taking framing as a strategic action in a discursive form (Pan & Kosicki, 2003), and in the context of the attribution theory of responsibilities, this research uses the “cascading activation” model (Entman, 2003, 2004) to explore how a framing contest was generated in the press. Analysis of the coverage shows that the intended government frame triggered a battle among the different major newspapers, leading editorials to shift their frame over the four days prior to the national elections. This research analyzes strategic contests in framing processes and contributes insight into the interactions among the different sides (government, parties, media, and citizens) to help bring about an understanding of the rebuttal effect of the government’s intended frame. It also helps to develop an understanding of the role of the media and the influence of citizens’ frames on media content.

Passarela estaiada com tabuleiro de madeira laminada protendida em módulos curvos

É inegável a importância do desenvolvimento de tecnologias de uso racional da madeira e de solução de problemas de transportes em nossas cidades, principalmente por causa do processo crescente de urbanização do mundo. Existe também, a necessidade estética de se unir à alta tecnologia, o belo. As passarelas estaiadas com tabuleiro de madeira laminada protendida, em módulos curvos, atendem a todas estas exigências. A construção de um protótipo permitiu que a realização de ensaios estáticos e dinâmicos, cujos resultados evidenciaram a viabilidade técnica e econômica de passarelas estaiadas usando madeira de reflorestamento, de tabuleiros compostos apenas por placas de madeira laminada protendida e da construção de placas curvas de madeira laminada protendida. Também foi possível comprovar que as vibrações induzidas por pedestres são a condição mais crítica de projeto. A investigação da perda de curvatura do tabuleiro demonstrou o sucesso do projeto, embora mais estudos ao longo do tempo sejam necessários. Baseando-se nos resultados experimentais, realizou-se a calibração do modelo numérico, que permitiu realizar simulações para determinar quais variáveis definem a resposta da passarela construída. Sugestões de procedimentos de elaboração e construção de passarelas, assim como de criação de norma brasileira específica para pontes e passarelas de madeira, são apresentadas.

Measurement of electrons from heavy-flavour hadron decays in p-Pb collisions at $\\sqrt{s_{NN}} = 5.02$ TeV using TPC and EMCal detectors with ALICE at LHC

Heavy-ion collisions are a powerful tool to study hot and dense QCD matter, the so-called Quark Gluon Plasma (QGP). Since heavy quarks (charm and beauty) are dominantly produced in the early stages of the collision, they experience the complete evolution of the system. Measurements of electrons from heavy-flavour hadron decay is one possible way to study the interaction of these particles with the QGP. With ALICE at LHC, electrons can be identified with high efficiency and purity. A strong suppression of heavy-flavour decay electrons has been observed at high $p_{m T}$ in Pb-Pb collisions at 2.76 TeV. Measurements in p-Pb collisions are crucial to understand cold nuclear matter effects on heavy-flavour production in heavy-ion collisions. The spectrum of electrons from the decays of hadrons containing charm and beauty was measured in p-Pb collisions at $\\sqrt = 5.02$ TeV. The heavy flavour decay electrons were measured by using the Time Projection Chamber (TPC) and the Electromagnetic Calorimeter (EMCal) detectors from ALICE in the transverse-momentum range $2 < p_ < 20$ GeV/c. The measurements were done in two different data set: minimum bias collisions and data using the EMCal trigger. The non-heavy flavour electron background was removed using an invariant mass method. The results are compatible with one ($R_ \\approx$ 1) and the cold nuclear matter effects in p-Pb collisions are small for the electrons from heavy-flavour hadron decays.

Estância Hidromineral de Águas de São Pedro (SP) e a construção de um espaço voltado ao termalismo

Este trabalho discorre sobre como o município de Águas de São Pedro, situado no centro do Estado de São Paulo, constituído na década de 1930 como um espaço voltado à atividade turística, destaca-se pelo potencial termal. Até hoje a estância hidromineral tem no turismo a principal atividade econômica; entretanto, nossa pesquisa busca compreender se o atrativo primordial do Município está atrelado às águas medicinais, ou se outras características singulares da pequena cidade, como tranquilidade, beleza paisagística e segurança, o tornam um lugar propício ao passeio. Buscamos entender por que, dentre tantas opções de cidades interioranas disponíveis em São Paulo, Águas de São Pedro é escolhida como local de residência fixa, lugar de estabelecimento de casas de veraneio, sendo palco de um grande crescimento imobiliário; assim, desvendamos quais fatores da estância são responsáveis por atrair os turistas e veranistas/turistas de segunda residência. Os dados da pesquisa foram obtidos por meio de pesquisa bibliográfica, observação direta, conversas informais, aplicação de questionários e entrevistas com vários atores socioeconômicos, tais como moradores, turistas, veranistas, rede hoteleira, comércios, imobiliárias e gestores municipais. Os resultados apontam que o município já passou por vários ciclos turísticos e, apesar de a estância hidromineral de Águas de São Pedro ser uma referência regional e até nacional de águas termais, e do poder público estar investindo para apresentar à sociedade um lugar cheio de qualidade de vida, símbolo de bem-estar, grande parcela dos turistas procura a cidade pelo passeio, gastronomia e compras, caracterizando um turismo excursionista, sem pernoite. Este é o motivo pelo qual a cidade vem passando por grande reestruturação paisagística, orquestrada pelo poder público e porque recebe incrementos do poder privado no que se refere à variedade de produtos e serviços ofertados. Percebemos que a maior parcela de moradores e turistas não têm o hábito de utilizar as águas medicinais, mesmo sabendo que a água sulfurosa, também chamada de \"Fonte da Juventude\", utilizada para ingestão e banhos terapêuticos, é a segunda melhor do mundo, superada apenas pela fonte de Pergoli, em Tabiano, na Itália (CAMARGO, 1990), porém o reconhecimento da Cidade como cidade das águas, cidade termal, cidade saúde, é resiliente.

When Wells Run Dry: Water and Tourism Along the Western Coast of Nicaragua

This dissertation uses a political ecology approach to examine the relationship between tourism development and groundwater in southwest Nicaragua. Tourism in Nicaragua is a booming industry bolstered by ‘unspoiled’ natural beauty, low crime rates, and government incentives. This growth has led to increased infrastructure, revenue, and employment opportunities for many local communities along the Pacific coast. Not surprisingly, it has also brought concomitant issues of deeper poverty, widening gaps between rich and poor, and competition over natural resources. Adequate provisions of freshwater are necessary to sustain the production and reproduction of tourism; however, it remains uncertain if groundwater supplies can keep pace with demand. The objective of this research is to assess water supply availability amidst tourism development in the Playa Gigante area. It addresses the questions: 1) are local groundwater supplies sufficient to sustain the demand for freshwater imposed by increased tourism development? and 2) is there a power relationship between tourism development and control over local freshwater that would prove inequitable to local populations? Integrating the findings of groundwater monitoring, geological mapping, and ethnographic and survey research from a representative stretch of Pacific coastline, this dissertation shows that diminishing recharge and increased groundwater consumption is creating conflict between stakeholders with various levels of knowledge, power, and access. Although national laws are structured to protect the environment and ensure equitable access to groundwater, the current scramble to secure water has powerful implications on social relations and power structures associated with tourism development. This dissertation concludes that marginalization due to environmental degradation is attributable to the nexus of a political promotion of tourism, poorly enforced state water policies, insufficient water research, and climate change. Greater technical attention to hydrological dynamics and collaboration amongst stakeholders are necessary for equitable access to groundwater, environmental sustainability, and profitability of tourism.

The pre-raphaelite female muse in Julia margaret cameron’s “idylls of the king” photographs

There has been little discussion of Julia Margaret Cameron’s Idylls of the King photographs over the past decade. My goal with this paper is to bring her Idylls of the King series back into discussion and address its success and relevance in both art history and literature. Scholars Helmut Gernsheim and Marylu Hill have questioned photography as a means to capture the imaginative content of Tennyson’s Arthurian stories and they declared Cameron’s photographs a failure. I argue that her theatrical style, use of props and costumes, obvious posing of her models, and nod to Victorian tableaux vivants capture the true essence of Tennyson’s epic. Her use of the Pre-Raphaelite female muse to portray the Arthurian characters of Elaine, Guinevere, and Vivien places her photographs in direct correlation with Pre-Raphaelite painting as well as popular literature. Her depictions of Tennyson’s epic poem are highly successful and I believe she achieved her personal goal of ennobling photography to the level of High Art.

Samurai Son and Exploring Cultural Myths Through Fantasy Fiction

Samurai Son is a story that captures the beauty, wonder and imagination of Japanese legends and mythology with some modern-day sensibilities and morals against the backdrop of medieval Japan. The story blends Shinto religion and stories about the tengu, bird-like wind spirits with a modern plotline in a Japanese fantasy universe. This world is filled with magic, demons, kami (magical spirits), gods and goddesses, samurai, ninja and martial arts.

Creating Beautiful Art: Challenging Comfort

Creating Beautiful Art: Challenging Comfort investigates the notion of beauty and its relationship to art while asking what is and can be art. Throughout the work, research supports the concept of beauty elevating art even with the use of atypical, unconventional, or mundane materials. Direct attention is given to this idea by completion of a Creative Capstone Project. I incorporated aesthetics with unconventional materials in order to challenge viewing comfort, as well as added value to the existing body of knowledge concerning beauty in contemporary art. The reflective section summarizes the importance of unconventional materials creating beauty in art in order to progress itself linearly by creating and reinventing the new.

Gender portrayals in food commercials: a content analysis of Spanish television advertisements

Content analysis of media messages from a gender perspective has a long tradition. In the particular field of advertising, most of the researches have been focused on generic samples of advertisements or on the advertising traditionally associated to a masculine or a feminine audience. However, few authors have analyzed gender contents in food advertising, in spite of the close relationship between gender structures and the social processes of food purchase and preparation. Thus, gender representations in food advertising broadcast in Spanish television are analyzed in this article. The main results obtained show clear gender differences. For example, there is a clear predominance of masculine voices over and a more balanced situation regarding protagonists. Besides, feminine protagonists are more frequently located indoors, portrayed as attractive women and connected to advertising claims related to beauty or to the healthy qualities of the product.

Plantas atípicas de Cymbalaria aequitriloba (Viv.) A.Chev. cultivadas en la comarca del Matarraña (Teruel)

Cymbalaria aequitriloba (Viv.) A.Chev., es una planta endémica del área Tirrénica (Sicilia, Córcega, Cerdeña y algunos puntos de Italia peninsular), que también alcanza las Islas Baleares. Ha sido encontrada en Torre de Arcas (Teruel) como una planta cultivada o accidental, presente en jardineras de floración invernal, constituyendo un elemento muy particular de la jardinería popular del Matarraña aragonés. Que sepamos, es la primera vez que se cita esta especie en España peninsular. Presenta una gran variabilidad morfológica lo que dificulta su identificación, no obstante es fácilmente diferenciable de C. muralis, la especie más cultivada en la zona. En esta región, tienen por costumbre remover la tierra tras la floración pues la planta pierde belleza, luego vuelve a aparecer en la siguiente estación por renuevo vegetativo o algunas plantas de semilla, motivo que no nos ha permitido estudiar plantas en fructificación. Todo Aragón, pero especialmente el Matarraña, tuvieron estrechos lazos comerciales con Italia durante el s. XIV-XV (principalmente exportando la lana), hecho que pudo favorecer el intercambio de materiales vegetales, como ya se ha podido constatar previamente con otras plantas similares.

Contradicciones del significado de la actividad físico-deportiva en las identidades corporales de las chicas adolescentes

Dentro de la estructura de género que impone la construcción social del cuerpo femenino, el sentimiento de inadecuación del propio cuerpo parece caracterizar la configuración de las identidades corporales femeninas en la adolescencia, motivando que la relación que establecen muchas chicas jóvenes con la actividad físico-deportiva sea compleja y a menudo problemática. Siguiendo un enfoque feminista post-estructuralista, este trabajo se centra en tres conflictos: 1) La actividad física para perder peso, instrumentalizada dentro de los discursos para alcanzar el ideal de belleza o luchar contra la obesidad; 2) El límite socialmente construido para las chicas deportistas entre lo que se considera un tono muscular atractivo y un cuerpo excesivamente musculoso; 3) Los procesos de sexualización y ansiedad física social debido a la exposición del cuerpo a una mirada masculina no deseada en diferentes contextos de práctica físico-deportiva.

Estética natural e ética ambiental, que relação?

This paper defends that environmental aesthetics provides a consistent basis for environmental philosophy, whereas aesthetic value plays an important role in the defense and preservation of natural areas. For several environmental philosophers the natural beauty is an inherent part of the ethical concern. Leopold states that “a thing is right when it tends to preserve the integrity, the balance and the beauty of the biotic community”. Notwithstanding, aesthetic value is still not a central issue in the environmental debate. On the other hand, the “positive aesthetics” (Allen Carlson), which is a recent approach that reevaluates “positively” natural beauty in the ethical context, obtains a core of objections. This paper sketches a few arguments defending the contiguity between environmental aesthetics and environmental ethics: (i) the emotional perception of inclusiveness and engagement on the aesthetics appreciation of nature; (ii) the feelings of grace and love toward nature inherent to the nature’s aesthetic appreciation which according Kant announces the moral feeling; (iii) the ecological knowledge of natural beauty in order to understand the full meaning of it, and that includes some natural entities seen as not beautiful.

A natureza : solo de conjunção da ética e da estética : fundamentos para a perspectivação do valor estético da natureza na acção ambiental

Tese de doutoramento, Filosofia (Filosofia da Natureza e do Ambiente), Universidade de Lisboa, Faculdade de Letras, 2016