Evolutionary history of the recruitment of conserved developmental genes in association to the formation and diversification of a novel trait

The origin and modification of novel traits are important aspects of biological diversification. Studies combining concepts and approaches of developmental genetics and evolutionary biology have uncovered many examples of the recruitment, or co-option, of genes conserved across lineages for the formation of novel, lineage-restricted traits. However, little is known about the evolutionary history of the recruitment of those genes, and of the relationship between them -for example, whether the co-option involves whole or parts of existing networks, or whether it occurs by redeployment of individual genes with de novo rewiring. We use a model novel trait, color pattern elements on butterfly wings called eyespots, to explore these questions. Eyespots have greatly diversified under natural and sexual selection, and their formation involves genetic circuitries shared across insects.

Screening of the Open Source Malaria Box Reveals an Early Lead Compound for the Treatment of Alveolar Echinococcosis.

The metacestode (larval) stage of the tapeworm Echinococcus multilocularis causes alveolar echinococcosis (AE), a very severe and in many cases incurable disease. To date, benzimidazoles such as albendazole and mebendazole are the only approved chemotherapeutical treatment options. Benzimidazoles inhibit metacestode proliferation, but do not act parasiticidal. Thus, benzimidazoles have to be taken a lifelong, can cause adverse side effects such as hepatotoxicity, and are ineffective in some patients. We here describe a newly developed screening cascade for the evaluation of the in vitro efficacy of new compounds that includes assessment of parasiticidal activity. The Malaria Box from Medicines for Malaria Venture (MMV), comprised of 400 commercially available chemicals that show in vitro activity against Plasmodium falciparum, was repurposed. Primary screening was carried out at 10 μM by employing the previously described PGI assay, and resulted in the identification of 24 compounds that caused physical damage in metacestodes. Seven out of these 24 drugs were also active at 1 μM. Dose-response assays revealed that only 2 compounds, namely MMV665807 and MMV665794, exhibited an EC50 value below 5 μM. Assessments using human foreskin fibroblasts and Reuber rat hepatoma cells showed that the salicylanilide MMV665807 was less toxic for these two mammalian cell lines than for metacestodes. The parasiticidal activity of MMV665807 was then confirmed using isolated germinal layer cell cultures as well as metacestode vesicles by employing viability assays, and its effect on metacestodes was morphologically evaluated by electron microscopy. However, both oral and intraperitoneal application of MMV665807 to mice experimentally infected with E. multilocularis metacestodes did not result in any reduction of the parasite load.

Operational maneuver from the sea.

Mode of access: Internet.

Strengthening support & recruitment of women & minorities to positions in education administration : a resource manual.

"December 1992"--T.p. verso.

Minority recruitment and retention in ARL libraries /

"September 1990."

The Agricultural Recruitment System : an employer's guide to hiring temporary agricultural workers.

Shipping list no.: 92-608-P.

Traffic maneuver problems of older drivers : final technical report /

"August 1993."

Development of vehicle rollover maneuver. Volume I. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Development of vehicle rollover maneuver. Volume II. Executive summary report.

National Highway Traffic Safety Administration, Washington, D.C.

The history and treatment of the diseases of the teeth, the gums, and the alveolar processes, with the operations which they respectively require...Observations on other diseases of the mouth...Artificial teeth /

Mode of access: Internet.

Marketing resource guide for recruitment and retention of providers in the early and periodic screening, diagnosis, and treatment program (EPSDT).

"August 1982"--P. [4] of cover.

Army TENCAP into the 21st century-- : bringing space to ground maneuver.

Mode of access: Internet.

The DOD Recruitment Program for Persons with Targeted Disabilities : we've put the disability behind us.

Shipping list no : 92-211-P.

DCEO, Department of Commerce & Economic Opportunity, recruitment information /

"1/11"--Colophon.