Miocene microflora and palaeoclimatic estimates of the Stetten section (Austria)

Pollen analyses have been proven to possess the possibility to decipher rapid vegetational and climate shifts in Neogene sedimentary records. Herein, a c. 21-kyr-long transgression-regression cycle from the Lower Austrian locality Stetten is analysed in detail to evaluate climatic benchmarks for the early phase of the Middle Miocene Climate Optimum and to estimate the pace of environmental change. Based on the Coexistence Approach, a very clear signal of seasonality can be reconstructed. A warm and wet summer season with c. 204-236 mm precipitation during the wettest month was opposed by a rather dry winter season with precipitation of c. 9-24 mm during the driest month. The mean annual temperature ranged between 15.7 and 20.8 °C, with about 9.6-13.3 °C during the cold season and 24.7-27.9 °C during the warmest month. In contrast, today's climate of this area, with an annual temperature of 9.8 °C and 660 mm rainfall, is characterized by the winter season (mean temperature: -1.4 °C, mean precipitation: 39 mm) and a summer mean temperature of 19.9 °C (mean precipitation: 84 mm). Different modes of environmental shifts shaped the composition of the vegetation. Within few millennia, marshes and salt marshes with abundant Cyperaceae rapidly graded into Taxodiaceae swamps. This quick but gradual process was interrupted by swift marine ingressions which took place on a decadal to centennial scale. The transgression is accompanied by blooms of dinoflagellates and of the green alga Prasinophyta and an increase in Abies and Picea. Afterwards, the retreat of the sea and the progradation of estuarine and wetland settings were a gradual progress again. Despite a clear sedimentological cyclicity, which is related to the 21-kyr precessional forcing, the climate data show little variation. This missing pattern might be due to the buffering of the precessional-related climate signal by the subtropical vegetation. Another explanation could be the method-inherent broad range of climate-parameter estimates that could cover small scale climatic changes.

Proportion of bryozoa, isopoda and ostracoda, and bryozoan species richness on the Antarctic continental shelf, slope and abyss

The Antarctic continental slope spans the depths from the shelf break (usually between 500 and 1000 m) to ~3000 m, is very steep, overlain by 'warm' (2-2.5 °C) Circumpolar Deep Water (CDW), and life there is poorly studied. This study investigates whether life on Antarctica's continental slope is essentially an extension of the shelf or the abyssal fauna, a transition zone between these or clearly distinct in its own right. Using data from several cruises to the Weddell Sea and Scotia Sea, including the ANDEEP (ANtarctic benthic DEEP-sea biodiversity, colonisation history and recent community patterns) I-III, BIOPEARL (Biodiversity, Phylogeny, Evolution and Adaptive Radiation of Life in Antarctica) 1 and EASIZ (Ecology of the Antarctic Sea Ice Zone) II cruises as well as current databases (SOMBASE, SCAR-MarBIN), four different taxa were selected (i.e. cheilostome bryozoans, isopod and ostracod crustaceans and echinoid echinoderms) and two areas, the Weddell Sea and the Scotia Sea, to examine faunal composition, richness and affinities. The answer has important ramifications to the link between physical oceanography and ecology, and the potential of the slope to act as a refuge and resupply zone to the shelf during glaciations. Benthic samples were collected using Agassiz trawl, epibenthic sledge and Rauschert sled. By bathymetric definition, these data suggest that despite eurybathy in some of the groups examined and apparent similarity of physical conditions in the Antarctic, the shelf, slope and abyssal faunas were clearly separated in the Weddell Sea. However, no such separation of faunas was apparent in the Scotia Sea (except in echinoids). Using a geomorphological definition of the slope, shelf-slope-abyss similarity only changed significantly in the bryozoans. Our results did not support the presence of a homogenous and unique Antarctic slope fauna despite a high number of species being restricted to the slope. However, it remains the case that there may be a unique Antarctic slope fauna, but the paucity of our samples could not demonstrate this in the Scotia Sea. It is very likely that various ecological and evolutionary factors (such as topography, water-mass and sediment characteristics, input of particulate organic carbon (POC) and glaciological history) drive slope distinctness. Isopods showed greatest species richness at slope depths, whereas bryozoans and ostracods were more speciose at shelf depths; however, significance varied across Weddell Sea and Scotia Sea and depending on bathymetric vs. geomorphological definitions. Whilst the slope may harbour some source populations for localised shelf recolonisation, the absence of many shelf species, genera and even families (in a poorly dispersing taxon) from the continental slope indicate that it was not a universal refuge for Antarctic shelf fauna.

Amount and patterns of beluga sightings in the eastern Beaufort Sea

An understanding of the adaptability of belugas (Delphinapterus leucas) to changing ice conditions is required to interpret and predict possible changes in habitat selection in response to projected loss of sea ice throughout the circumpolar Arctic. We analyzed beluga observations made during spring aerial surveys for ringed seals conducted from 1975 to 1979 in the eastern Beaufort Sea. Despite interannual variability in the extent and distribution of sea ice, belugas consistently selected areas with water depths of 200-500 m and heavy ice concentrations (8/10 to 10/10) while areas of open water to light ice concentrations (0/10 to 1/10) were not selected. Belugas were also found in proximity to regions with >0.5 degrees seafloor slope which include the continental slope and other areas with the potential for oceanographic upwellings. In most years (4 of 5), fast-ice edges and coastal areas were not selected. In the lightest ice year analyzed, belugas showed less specificity in habitat selection as their distribution expanded and shifted shoreward to fast-ice edges. The observed distribution is discussed in terms of predator-prey relationships particularly with reference to beluga feeding on polar cod (Boreogadus saida). More research is required to examine and compare possible changes in distribution since the late 1970s and to investigate the factors driving the patterns described.

Pollen record and age determination of a sediment core from the Kara Sea

AMS-14C dated sediment cores from the Ob and Yenisei estuaries and the adjacent inner Kara Sea were investigated to determine the siliclastic and organic carbon fluxes and their relationship to paleoenvironmental changes. The variability of sediment fluxes during Holocene times is related to the post-glacial sea-level rise and changes in river discharge and coastal erosion input. Whereas during the late/middle Holocene most of the terrigenous sediments were deposited in the estuaries and the areas directly off the estuaries, huge amounts of sediments accumulated on the Kara Sea shelf farther north during the early Holocene before about 9 Cal. kyrs. BP. The maximum accumulation at that time is related to the lowered sea level, increased coastal erosion, and increased river discharge due to the final stage of mountain deglaciation of the Putoran Massif. Increased supply of Yenisei-derived material indicated by peak magnetic susceptibility values probably occurred in climate-related pulses culminating near 11, 10, and 9 Cal. kyrs. BP. As sea level rose, the main Holocene depocenter migrated southward. Based on hydrogen index values and n-alkanes, the organic matter is predominantly of terrigenous origin. Maximum accumulation rates of 1.5 to more than 6 g/cm2/y occurred in the early Holocene sediments, suggesting more humid climatic conditions with an increased vegetation cover in the source area at that time. In general, high organic carbon accumulation rates characterize the estuaries and the inner Kara Sea as important sink for terrigenous organic carbon. A high-resolution record of Holocene variability of magnetic susceptibility (MS) in an AMS14C-dated sediment core from the northern Yenisei estuary may indicate natural variability of Arctic climate change and river discharge on a centennial to millenial time scale. Short-term maxima in MS probably related to warmer climate, enhanced precipitation, intensified weathering/erosion and increased river discharge, display a frequency of about 300 to 700 years.

(Table 2) Concentrations of polychlorinated naphthalene congeners in blubber samples from whales, seals and porpoises between 1986-2009

A selection of PCN congeners was analyzed in pooled blubber samples of pilot whale (Globicephala melas), ringed seal (Phoca hispida), minke whale (Balaenoptera acutorostrata), fin whale (Balaenoptera physalus), harbour porpoise (Phocoena phocoena), hooded seal (Cystophora cristata) and Atlantic whitesided dolphin (Lagenorhynchus acutus), covering a time period of more than 20 years (1986-2009). A large geographical area of the North Atlantic and Arctic areas was covered. PCN congeners 48, 52, 53, 66 and 69 were found in the blubber samples between 0.03 and 5.9 ng/g lw. Also PCBs were analyzed in minke whales and fin whales from Iceland and the total PCN content accounted for 0.2% or less of the total non-planar PCB content. No statistically significant trend in contaminant levels could be established for the studied areas. However, in all species except minke whales caught off Norway the lowest Sum PCN concentrations were found in samples from the latest sampling period.

Representative and whole rock analyses of rocks from Filchnerfjella, Antarctica

The geological history of Filchnerfjella and surrounding areas (2°E to 8°E) in central Dronning Maud Land, East Antarctica, is constructed from metamorphic and igneous petrology, and structural investigations. The geology of Filchner-fjella consists mainly of metamorphic rocks accompanied by intrusive rocks. Two stages of metamorphism can be recognized in this area. The earlier stage metamorphism is defined as a porphyroblast stage (garnet, hornblende, and sillimanite stable), and the later one is recognized as a symplectic stage (orthopyroxene and cordieritestable). Taking metamorphic textures and geothermobarometries into account, the rocks experienced an early high-P/medium-T followed by a low-P and high-T stage. Partial melting took place during the low-P/high-T stage, because probable melt of leucocratic gneiss contains cordierite. The field relationships and petrography of the syenite at Filchnerfjella are similar to those of post-tectonic plutons from central Dronning Maud Land, and most of the post-tectonic intrusive rocks have within-plate geochemical features. The structural history in Filchnerfjella and surrounding areas can be divided into the Pan-African stage and the Meso to Cenozoic stage that relates to the break-up of Gondwana.

Mineral composition, major element oxides and trace element concentration of sediments from ODP Leg 180 sites

Middle Miocene to Holocene fine-grained argillaceous sediments (clays, claystones/muds, and mudstones), which volumetrically dominated the sediment recovery in the Woodlark Basin during Leg 180, were chemically analyzed for major elements, trace elements, and some rare earth elements by X-ray fluorescence. Selected samples also underwent X-ray diffraction (XRD) analysis for mineral determination. The results shed light on sediment provenance when combined with shipboard sediment descriptions, smear slide study, and XRD. The oldest sediments recovered (Site 1108) of middle-late Miocene age include volcanogenic muds with distinctive high MgO and K2O, indicative of a relatively basic calc-alkaline source related to an inferred Miocene forearc succession. The forearc basement, composed of diabase and basalt, was locally exposed (Site 1109) and eroded in the late Miocene (<5.4-9.93 Ma), giving rise to fluvial conglomerates (Sites 1109, 1115, and 1118). Chemically distinctive fine-grained claystones and siltstones (with relatively high Ti, low K) are compatible with derivation from tropically weathered basic igneous rocks, correlated with the Paleogene Papuan ophiolite. Overlying latest Miocene-Pleistocene fine-grained sediments throughout the Woodlark Basin were partly derived from calc-alkaline volcanic sources. However, relatively high abundances of Al2O3 and related element oxides (K2O and Na2O) and trace elements (e.g., Rb and Y) reflect an additional terrigenous input throughout the basin, correlated with pelitic metamorphic rocks exposed on Papua New Guinea and adjacent areas. In addition, sporadic high abundances of Cr and Ni, some other trace metals, and related minerals (talc, crysotile, and chlorite) reflect input from an ophiolitic terrain dominated by ultramafic rocks, correlated with the Paleogene Papuan ophiolite. The source areas possibly included serpentinized ultramafic ophiolitic rocks exposed in the Papua New Guinea interior highlands. Chemical evidence further indicates that fine-grained terrigenous sediment reached the Woodlark Basin throughout its entire late Miocene-Holocene history. Distinctive high-K volcanogenic muds rich in tephra and volcanic ash layers that appear at <2.3 Ma (Sites 1109 and 1115) are indicative of high-K calc-alkaline volcanic centers, possibly located in the Dawson Strait, Moresby Strait, or Dobu Seamount area. Chemical diagenesis of fine-grained sediments within the Woodlark Basin is reflected in clay neomorphism and localized formation of minerals including dolomite, ankerite, and zeolite but has had little effect on the bulk chemical composition of most samples.

Table 2: Areal distribution and specfic mass budget of Hintereisferner

Results of the direct, glaciological determination of the mass budget of Hintereisferner and Kesselwandferner in the Ötztal Alps are summarized for the years 1977/78-1980/81. Tabulations of budget quantities, accumulation and ablation areas are supplemented by graphs of altitudinal and areal distribution of mass balance and by examples of the seasonal course of ablation.

Seawater carbonate chemistry and Ruditapes decussatus biological processes during experiments, 2011

We investigated the effects of ocean acidification on juvenile clams Ruditapes decussatus (average shell length 10.24 mm) in a controlled CO2 perturbation experiment. The carbonate chemistry of seawater was manipulated by diffusing pure CO2, to attain two reduced pH levels (by -0.4 and -0.7 pH units), which were compared to unmanipulated seawater. After 75 days we found no differences among pH treatments in terms of net calcification, size or weight of the clams. The naturally elevated total alkalinity of local seawater probably contributed to buffer the effects of increased pCO2 and reduced pH. Marine organisms may, therefore, show diverse responses to ocean acidification at local scales, particularly in coastal, estuarine and transitional waters, where the physical-chemical characteristics of seawater are most variable. Mortality was significantly reduced in the acidified treatments. This trend was probably related to the occurrence of spontaneous spawning events in the control and intermediate acidification treatments. Spawning, which was unexpected due to the small size of the clams, was not observed for the pH -0.7 treatment, suggesting that the increased survival under acidified conditions may have been associated with a delay in the reproductive cycle of the clams. Future research about the impacts of ocean acidification on marine biodiversity should be extended to other types of biological and ecological processes, apart from biological calcification.

Chemical composition of sepiolites and palygorskites from bottom sediments of the Eastern Indian

In fault zones of the East Indian Ridge and adjacent areas of ocean floor almost monomineral sepiolite- and palygorskite clays have been found. They have been studied by a complex of optical and physical methods. Formation of authigenic sepiolites and palygorskite had occurred under influence of Mg- and Si-rich hydrothermal solutions by metasomatic replacement of montmorillonite clays, or by precipitation from saturated solutions in cracks of various rocks.

Abundance and Biomass from heterotrophic bacteria, Synechococcus, Prochlorococcus and Virus in the eastern Mediteranean Sea in October 2008 during SES_GR2

The HCMR_SES_LAGRANGIAN_GR2_ MICROBIAL PARAMETERS dataset is based on samples collected in the framework of the project SESAME, in the North Aegean Sea during October 2008. The objectives were to measure the standing stocks and calculate the production of the microbial compartment of the food web, describe the vertical distribution pattern and characterize its structure and function through the water column as influenced by the BSW. Heterotrophic bacteria, Synechococcus, Prochlorococcus and Virus abundance: Subsamples for virus, heterotrophic bacteria and cyanobacteria (Synechococcus spp. and Prochlorococcus spp.) counting were analyzed using a FACSCalibur (Becton Dickinson) flow cytometer equipped with a standard laser (488 nm) and filter set and using deionized water as sheath fluid. Fluorescent beads with a diameter of 0.97 µm (Polysciences) were added to each sample as an internal standard, and all parameters were normalized to the beads and expressed as relative units. SYBRGreen I stain (Molecular Probe) was used to stain viral and heterotrophic bacterial DNA. Viruses were counted according to (Brussaard 1984). In order to avoid bulk consentrations of viruses samples we dilluted to Tris-EDTA (pH=8,0) buffer to a final sollution of 1/5 to 1/100. Total abundance and nucleid content classes were calculated using the Paint-A-Gate software (Becton Dickinson). Heterotrophic Nanoflagellate abundance: Subsamples (30-150 ml) were concentrated on 25mm black polycarbonate filters of porosity 0.6?m and stained with DAPI for 10 min (Porter and Feig 1980). Under epifluorescence microscopy heterotrophic nanoflagellates (HNAN) were distinguished using UV and blue excitation and enumerated. Nanoflagellates were classified in size categories and the biovolume was calculated. Ciliate abundance: For ciliate identification and enumeration, 100-3000 ml samples were left for 24h-4d for sedimentation and then observed under an inverted microscope. Ciliates were counted, distinguished into size-classes and major taxonomic groups and identified down to genus or species level where possible (Pitta et al. 2005). Heterotrophic bacteria, Synechococcus, Prochlorococcus bacteria: Subsamples for virus, heterotrophic bacteria and cyanobacteria (Synechococcus spp. and Prochlorococcus spp.) counting were analyzed using a FACSCalibur (Becton Dickinson) flow cytometer equipped with a standard laser (488 nm) and filter set and using deionized water as sheath fluid. Fluorescent beads with a diameter of 0.97 µm (Polysciences) were added to each sample as an internal standard, and all parameters were normalized to the beads and expressed as relative units. SYBRGreen I stain (Molecular Probe) was used to stain viral and heterotrophic bacterial DNA. Viruses were counted according to (Brussaard 1984). In order to avoid bulk consentrations of viruses samples we dilluted to Tris-EDTA (pH=8,0) buffer to a final sollution of 1/5 to 1/100. Total abundance and nucleid content classes were calculated using the Paint-A-Gate software (Becton Dickinson). Abundance data were converted into C biomass using 250 fgC cell-1 (Kana & Glibert 1987) for Synechococcus, 50 fgC cell-1 (Campbell et al. 1994) for Prochlorococcus and 20fgC cell-1 (Lee & Fuhrman 1987) for heterotrophic bacteria. Heterotrophic Nanoflagellate biomass: Subsamples (30-150 ml) were concentrated on 25mm black polycarbonate filters of porosity 0.6µm and stained with DAPI for 10 min (Porter and Feig 1980). Under epifluorescence microscopy heterotrophic nanoflagellates (HNAN) were distinguished using UV and blue excitation and enumerated. Nanoflagellates were classified in size categories and the biovolume was calculated. Abundance data were converted into C biomass using 183 fgC µm**3 (Caron et al. 1995). Ciliate biomass: For ciliate identification and enumeration, 100-3000 ml samples were left for 24h-4d for sedimentation and then observed under an inverted microscope. Ciliates were counted, distinguished into size-classes and major taxonomic groups and identified down to genus or species level where possible (Pitta et al. 2005). Ciliate cell sizes were measured and converted into cell volumes using appropriate geometric formulae using image analysis. For biomass estimation, the conversion factor 190 fgC µm**3 was used (Putt and Stoecker 1989).

Table 1: Mass budget of Hintereisferner and Kesselwandferner

Results of the direct, glaciological determination of the mass budget of Hintereisferner and Kesselwandferner in the Ötztal Alps are summarized for the years 1075/76-1977/78. Tabulations of budget quantities, accumulation and ablation areas are supplemented by graphs of altitudinal, and areal distribution of mass balance and by examples of the seasonal course of ablation.

Isopoda E(S100) and surface sediment characteristics of ANDEEP I-III stations in the Southern Ocean deep sea

Shallow marine benthic communities around Antarctica show high levels of endemism, gigantism, slow growth, longevity and late maturity, as well as adaptive radiations that have generated considerable biodiversity in some taxa1. The deeper parts of the Southern Ocean exhibit some unique environmental features, including a very deep continental shelf2 and a weakly stratified water column, and are the source for much of the deep water in the world ocean. These features suggest that deep-sea faunas around the Antarctic may be related both to adjacent shelf communities and to those in other oceans. Unlike shallow-water Antarctic benthic communities, however, little is known about life in this vast deep-sea region2, 3. Here, we report new data from recent sampling expeditions in the deep Weddell Sea and adjacent areas (748-6,348 m water depth) that reveal high levels of new biodiversity; for example, 674 isopods species, of which 585 were new to science. Bathymetric and biogeographic trends varied between taxa. In groups such as the isopods and polychaetes, slope assemblages included species that have invaded from the shelf. In other taxa, the shelf and slope assemblages were more distinct. Abyssal faunas tended to have stronger links to other oceans, particularly the Atlantic, but mainly in taxa with good dispersal capabilities, such as the Foraminifera. The isopods, ostracods and nematodes, which are poor dispersers, include many species currently known only from the Southern Ocean. Our findings challenge suggestions that deep-sea diversity is depressed in the Southern Ocean and provide a basis for exploring the evolutionary significance of the varied biogeographic patterns observed in this remote environment.

Concentrations of particulate organic carbon in the Kara Sea and in the Obskaya Guba (Ob River estuary) in September 1993

Contents and distribution of particulate lipids were studied by thin-layer chromatography technique with flame ionization detection (Iatroscan TH-10) along the transect from the Ob River towards the Kara Sea. Lipid contents range from 18.4 to 266 µg/l with, average 84.97 µg/l, which comprises from 4.06 to 58.32 % of total particulate organic matter. Principal constituents of particulate lipids are hydrocarbons (32.14 % of total lipids on the average), polar compounds (29.85 %), wax and sterol esters (13.04 %), and mono- and diglycerides (12.52 %). Secondary components are presented by fatty acid esters (5.14 %), free fatty acids (4.56 %), triglycerides (2.32 %), and sterols (1.04 %). Specific composition of particulate lipids along the Ob River - Kara Sea transect is formed under strong impact of river run-off. Particulate lipid composition reflects differences between processes of organic matter transformation in estuarine and marine parts of the transect, as well as peculiarities of species composition of Arctic living organisms.