A simple method based on ICP-MS for estimation of background levels of arsenic, cadmium, copper, manganese, nickel, lead, and selenium in blood of the Brazillian population

Throughout the world, biomonitoring has become the standard for assessing exposure of individuals to toxic elements as well as for responding to serious environmental public health problems. However, extensive biomonitoring surveys require rapid and simple analytical methods. Thus, a simple and high-throughput method is proposed for the determination of arsenic (As), cadmium (Cd), copper (Cu), manganese (Mn), nickel (Ni), lead (Pb), and selenium (Se) in blood samples by using inductively coupled plasma–mass spectrometry (ICPMS). Prior to analysis, 200 ml of blood samples was mixed with 500 ml of 10% v/v tetramethylammonium hydroxide (TMAH) solution, incubated for 10 min, and subsequently diluted to 10 ml with a solution containing 0.05% w/v ethylenediamine tetraacetic acid (EDTA) + 0.005% v/v Triton X-100. After that, samples were directly analyzed by ICP-MS (ELAN DRC II). Rhodium was selected as an internal standard with matrix-matching calibration. Method detection limits were 0.08, 0.04, 0.5, 0.09, 0.12, 0.04, and 0.1 mg//L for As, Cd, Cu, Mn, Ni, Pb, and Se, respectively. Validation data are provided based on the analysis of blood samples from the trace elements inter-\comparison program operated by the Institut National de Santé Publique du Quebec, Canada. Additional validation was provided by the analysis of human blood samples by the proposed method and by using electrothermal atomic absorption spectrometry (ETAAS). The method was subsequently applied for the estimation of background metal blood values in the Brazilian population. In general, the mean concentrations of As, Cd, Cu, Mn, Ni, Pb, and Se in blood were 1.1, 0.4, 890, 9.6, 2.1, 65.4, and 89.3 mg/L, respectively, and are in agreement with other global populations. Influences of age, gender, smoking habits, alcohol consumption, and geographical variation on the values were also considered. Smoking habits influenced the levels of Cd in blood. The levels of Cu, Mn, and Pb were significantly correlated with gender, whereas Cu and Pb were significantly correlated with age. There were also interesting differences in Mn and Se levels in the population living in the north of Brazil compared to the south.

Eficácia de um gel com reduzida concentração de fluoreto suplementado com hexametafosfato de sódio sobre o processo de desmineralização do esmalte dentário: estudo in vitro

Pós-graduação em Ciência Odontólogica - FOA

Women s Representation in Trimalchio s Feast and The Great Gatsby

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desgaste e rugosidade superficial de um cimento de ionômero de vidro nanoparticulado

To compare the abrasion wear resistance and superficial roughness of different glass ionomer cements used as restorative materials, focusing on a new nanoparticulate material. Material and Method: Three glass ionomer cements were evaluated: Ketac Molar, Ketac N100 and Vitremer (3M ESPE, St. Paul, MN, USA), as well as the Filtek Z350 (3M ESPE, St. Paul, MN, USA). For each material were fabricated circular specimens (n=12), respecting the handling mode specified by the manufacturer, which were polished with sandpaper disks of decreasing grit. The wear was determined by the amount of mass (M) lost after brushing (10,000 cycles) and the roughness (Ra) using a surface roughness tester. The difference between the Minitial and Mfinal (ΔM) as well as beroughness of aesthetic restorative materials: an in vitro comparison. SADJ. 2001; 56(7): 316-20. 11. Yip HK, Peng D, Smales RJ. Effects of APF gel on the physical structure of compomers and glass ionomer cements. Oper. Dent. 2001; 26(3): 231-8. 12. Ma T, Johnson GH, Gordon GE. Effects of chemical disinfectants on the surface characteristics and color of denture resins. J Prosthet Dent 1997; 77(2): 197-204. 13. International organization for standardization. Technical specification 14569-1. Dental Materials – guidance on testing of wear resistance – PART I: wear by tooth brushing. Switzerland: ISO; 1999. 14. Bollen CML, Lambrechts P, Quirynen M. Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. Dent Mater.1997; 13(4): 258-9. 15. Kielbassa AM, Gillmann C, Zantner H, Meyer-Lueckel H, Hellwig E, Schulte-Mönting J. Profilometric and microradiographic studies on the effects of toothpaste and acidic gel abrasivity on sound and demineralized bovine dental enamel. Caries Res. 2005; 39(5): 380-6. 16. Tanoue N, Matsumara H, Atsuta M. Wear and surface roughness of current prosthetic composites after toothbrush/dentifrice abrasion. J Prosthet Dent. 2000; 84(1): 93-7. 17. Heath JR, Wilson HJ. Abrasion of restorative materials by toothpaste. J Oral Rehabil. 1976; 3(2): 121-38. 18. Frazier KB, Rueggeberg FA, Mettenburg DJ. Comparasion of wearresistance of class V restorative materials. J Esthet Dent. 1998; 10(6): 309-14. 19. Momoi Y, Hirosakil K, Kohmol A, McCabe JF. In vitro toothebrushdentifrrice abrasion of resin-modified glass ionomers. Dent Mater. 1997; 13(2): 82-8. 20. Turssi CP, Magalhães CS, Serra MC, Rodrigues Jr.AL. Surface roughness assessment of resin-based materials during brushing preceded by pHcycling simulations. Oper Dent. 2001; 26(6): 576-84. 21. Wang L, Cefaly DF, Dos Santos JL, Dos Santos JR, Lauris JR, Mondelli RF, et al. In vitro interactions between lactic acid solution and art glassionomer cements. J Appl Oral Sci. 2009; 17(4): 274-9. 22. Carvalho FG, Fucio SB, Paula AB, Correr GM, Sinhoreti MA, PuppinRontani RM. Child toothbrush abrasion effect on ionomeric materials. J Dent Child (Chic). 2008; 75(2): 112-6. 23. Coutinho E, Cardoso MV, De Munck J, Neves AA, Van Landuyt KL, Poitevin A, et al. Bonding effectiveness and interfacial characterization of a nano-filled resin-modified glass-ionomer. Dent Mater. 2009; 25(11): 1347-57. tween Rainitial and Rafinal (ΔRa) were also used for statistical analysis (α=0.05). Results: Except for the composite, significant loss of mass was observed for all glass ionomer cements and the ΔM was comparable for all of them. Significant increase in roughness was observed only for Vitremer and Ketac N100. At the end of the brushing cycle, just Vitremer presented surface roughness greater than the composite resin. Conclusion: All glass ionomer cements showed significant weight loss after 10,000 cycles of brushing. However, only Vitremer showed an increase of roughness greater than the Z350 resin, while the nanoparticulate cement Ketac N100 showed a smooth surface comparable to the composite.

Validation of the Geriatric Oral Health Assessment Index in complete denture wearers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produtividade e características físico-químicas de ora-pro-nobis sob adubação orgânica

Pós-graduação em Agronomia (Horticultura) - FCA

Seletividade de herbicidas inibidores do fotossistema II para cultivares de cana-de-açúcar

Pós-graduação em Agronomia (Agricultura) - FCA

Sensores eletroquímicos à base de nanomateriais carbonáceos e catalisadores biomiméticos para determinação de tetraciclina em diferentes tipos de amostras

Pós-graduação em Química - IQ

O sujeito-comprimido

Is it possible to encapsulate in a pill the benefits of an analytical treatment? Quickly suspending the symptoms? Since the nineteenth century psychiatry has offered to the so called mentally ill "medieval forms" of treatment, as is the case of institutional admissions, the straightjacket, lobotomies, shock treatments, etc. What has changed since the mid-twentieth century advent of chlorpromazine, the first psychoactive drug, and the "psychopharmacological revolution"? Today with the Psychiatric Reform mental institutions admissions are being gradually abandoned, yet we see the rise of psychoactive drugs as protagonists in mental health treatment. This theoretical essay is an extension of a master's thesis in progress on the long-term use of psychotropic drugs. It falls within the field of mental health, specifically in the debate on the medicalization of society in contemporary processes of subjectivation of the psychiatric clinics and the treatment modalities currently offered to individuals in their suffering-existence. Thus, we seek to problematize this degrading psychiatric clinics, which produces what we call the pill-subject.

Quantificação de parâmetros da dinâmica da bandeja do basquetebol por variáveis temporais do salto e antropométricas

The objective of this research study was quantify the power, force of vertical impulse, jumping height and the relation of the power output during the execution of the layup in basketball, by variables associated to anthropometric data. The study was carried with seven basketball players, university students, male, from the Universidade Estadual Paulista Julio Mesquita Filho (Unesp) basketball team, from Bauru City. The players were an age of 20,7 ± 2,4 years old, body weight of 77,1 ± 9,4 kg, body fat of 14,7 ± 3,6%, lean mass of 65,5 ± 6,2 kg and height of 180,0 ± 5,0 cm. Each player did the ability of the layup three times, with a previous time to adapt. The kinetic and kinematic values was obtained from the data of a system that consists of photocells and a contact carpet from a system called JumpSystemâ (CEFISE), using after that equations of UVRM and impulse. The photocells was dispose to capture the previous run-up, as well the speedy in the time of two steps of the layup, and it was also synchronized with the contact carpet in the last step, to preserve the exactly time of the propulsion of jump. In this way, the values and the kinetic variables found was vV (2,94 ± 0,3 m's-1), vH (11,3 ± 3,4 m's-1), vI (11,8 ± 3,2 m's-1), time of contact (0,214 ± 0,2 s), angle of jump (15,9 ± 6,26 graus) and H (44,3 ± 8,9 cm). The variables and the kinematic values found was FimpV (974,9 ± 119,03 N, PP (3112,9 ± 633,9 W) and PM (1556,4 ± 316,9 W). To better understand the ability of layup in basketball, as the kinetic variables, kinematic variables and anthropometrics that determine the performance, the study did correlations between dV (H) with vV, vH, vI, angle, FimpV, PP e PM; FimpV, PP e PM com with the kinetic variables of vV, vH, vI, angle, and anthropometric variables of PC and height, and correlations of PP e PM with %G and MM. So, significant results was found between the correlations of dV with FimpV (R2 = 0,81)...

Refractive errors in a Brazilian population: age and sex distribution

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diurnal effects on spearmint oil yields and composition

'Native'spearmint (Mentha spicata L.) is one of the two spearmint species grown commercially in the United States and other countries for essential oil production. The two major constituents of spearmint oil are carvone and limonene. It is not known if the essential oil yield (content) and composition of spearmint oil are affected by diurnal variation, and when it would be the best time for harvesting flowering spearmints within a 24h period. Therefore, the objective of this study was to evaluate the effect of diurnal variation on yield and composition of spearmint 'Native'essential oil for the environmental conditions of Northern Wyoming, at elevation 1170 m above the sea level. The experiment was conducted on a 3-year old well established spearmint plantation. The harvest times were every 2 h within a 24-h period: 7:00 AM, 9:00 AM, 11:00AM, 1:00 PM, 3:00 PM, 5:00 PM, 7:00 PM, 9:00 PM, 11:00 PM, 1:00 AM, 3:00 AM, and 5:00 AM. Essential oil yield varied from 0.96 to 1.47 g of oil per 100 g of dry herbage; the maximum oil yield was obtained at 9:00 AM and the minimum at 7:00 PM. The concentration of carvone in the oil varied from 44.1% (at 1:00 PM) to 66.4% (at 9:00 PM) of the total oil. However, the yield of carvone (a function of oil yield and carvone concentration in the oil) was the highest at 3:00 AM and the lowest at 1:00 PM. The concentration of limonene (10.7-15.8% of the oil) was the highest at 7:00 PM and the lowest at 1:00 PM, whereas the yield of limonene was the highest at 9:00 PM. For best essential oil yields, flowering spearmint should be harvested at around 9:00 AM. However, to obtain oil with high carvone concentration, spearmint should be harvested at 9:00 PM. Harvests at 1:00 PM would result in spearmint oil with low concentrations of both carvone and limonene, and hence, should be avoided. (C) 2014 Elsevier B.V. All rights reserved.

The interval between the emergence of pharmacologically synchronized ovarian follicular waves and ovum pickup does not significantly affect in vitro embryo production in Bos indicus, Bos taurus, and Bubalus bubalis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Factors associated with sarcopenia in subjects aged 80 years and over

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Grinding process monitoring based on electromechanical impedance measurements

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)