Development of 18 Polymorphic Microsatellite Markers for Vinca minor (Apocynaceae) via 454 Pyrosequencing

• Premise of the study: Polymorphic microsatellite markers were developed in Vinca minor (Apocynaceae) to evaluate the level of clonality, population structure, and genetic diversity of the species within its native and introduced range. • Methods and Results: A total of 1371 microsatellites were found in 43,565 reads from 454 pyrosequencing of genomic V. minor DNA. Additional microsatellite loci were mined from publicly available cDNA sequences. After several rounds of screening, 18 primer pairs flanking di-, tri-, or tetranucleotide repeats were identified that revealed high levels of genetic diversity in two native Italian populations, with two to 11 alleles per locus. Clonal growth predominated in two populations from the introduced range in Germany. Five loci successfully cross-amplified in three additional Vinca species. • Conclusions: The novel polymorphic microsatellite markers are promising tools for studying clonality and population genetics of V. minor and for assessing the historical origin of Central European populations.

Congreso internacional de creatividad : espacios para la creatividad : IV encuentro de profesores e investigadores en el campo de la creatividad : la evaluaci??n de la creatividad. Libro de res??menes. Del 18 al 20 de septiembre de 2003, La Manga del Mar Menor

Incluye relaci??n de participantes con sus direcciones electr??nicas

VIII seminario internacional de praxiolog??a motriz, 16,17 y 18 de octubre de 2003. Facultad de Educaci??n, sal??n de actos

Seminario que pretende difundir y clarificar la Praxiolog??a Motriz como nueva ciencia que va m??s all?? del an??lisis del deporte. Introducida por el profesor Pierre Parleb??s, de la Universidad de Par??s, que pronuncia la conferencia inaugural titulada: Lectura profunda de los cuadros de Brueguel El Viejo y Stella desde la Praxiolog??a Motriz. Aborda, adem??s, la intencionalidad de la conducta motriz estableciendo las coordenadas clarificadoras sobre conceptos y terminolog??a de la PM y la organizaci??n de su conocimiento.