924 resultados para synchronous


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Members of the subfamily Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as preferential sites for primary replication. However, bovine herpesvirus 5 (BoHV5) is neurotropic and neuroinvasive and responsible for meningoencephalitis in cattle and in animal models. A related virus, BoHV1 has also been occasionally implicated in natural cases of neurological infection and disease in cattle. The aim of the present study was to assess the in vitro effects of BoHV1 and BoHV5 replication in neuron-like cells. Overall, cytopathic effects, consisting of floating rounded cells, giant cells and monolayer lysis, induced by both viruses at 48 h postinfection (p.i.) resulted in a loss of cell viability and high virus titres (r = 0.978). The BoHV1 Cooper strain produced the lowest titres in neuron-like cells, although viral DNA was detected in infected cells during all experiments. Virus replication in infected cells was demonstrated by immunocytochemistry, flow cytometry and qPCR assays. BoHV antigens were better visualized at 48 h p.i. and flow cytometry analysis showed that SV56/90 and Los Angeles antigens were present at higher levels. In spite of the fact that BoHV titres dropped at 48 h p.i, viral DNA remained detectable until 120 h p.i. Sensitive TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) and annexin V assays were used to identify apoptosis. BoHV5 induced death in approximately 50 % of cells within 24 h p.i., similar to what has been observed for BoHV1 Los Angeles. Infection with the BoHV1 Cooper strain resulted in 26.37 % of cells being in the early stages of apoptosis; 63.69 % of infected cells were considered viable. Modulation of mitochondrial function, as measured by mitochondrial membrane depolarization, was synchronous with the virus replication cycle, cell viability and virus titres at 48 h p.i. Our results indicate that apoptosis plays an important role in preventing neuronal death and provides a bovine-derived in vitro system to study herpesvirus-neuron interactions.

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Aims: Hypochlorous (HOCl) and hypobromous (HOBr) acids are among the most powerful oxidants produced by the innate immune cells. Albumin is the predominant protein in most body fluids and is considered the most important antioxidant of blood plasma. Study Design: Oxidation of bovine albumin (BSA) and study of its structural and functional alterations. Place and Duration of Study: Faculty of Science and Faculty of Pharmaceutical Science, University of the State of Sao Paulo UNESP, between June and December 2012. Methodology: BSA was oxidized with excess of HOCl or HOBr and its structural and functional alterations were analyzed by spectroscopic techniques as UV-Vis absorption, intrinsic and synchronous fluorescence, fluorescence quenching, Rayleigh scattering and circular dichroism. Results: Both oxidants were able to deplete the intrinsic fluorescence of BSA, but HOBr was more effective than HOCl. The alterations in the synchronous fluorescence, UV-Vis absorption, and the appearance of a fluorescence band centered at 450 nm confirmed the difference between the oxidants. The oxidation did not induce aggregation of BSA as measured by Rayleigh scattering. The far-UV circular dichroism spectra showed a loss in the helical content and the near-UV-circular dichroism showed an alteration in the tertiary structure; HOBr was the more effective of the oxidants in this case. However, the oxidations did not induce significant alterations in the binding capacity of BSA, which was evaluated using hydrophobic (norfloxacin) and hydrophilic (ascorbic acid) drugs. Conclusion: These results suggest that, although highly susceptible to oxidation, the alterations did not inhibit BSAâs physiological function as a transport protein.

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One tool that has been in evidence, especially among young people, is Facebook. It can be classified as a synchronous communication tool that allows communities of people with similar interests to discuss and exchange experiences in real time, promoting the sharing of information and the creation of collective knowledge, even if they being in different parts of the globe. In this paper we show that Facebook can be used as an educational tool to aid the work done in the classroom and the impact of creating closed groups in online social networking for educational purposes. The survey was conducted with a group of students at a private school in Bauru/SP. We investigated the interaction profile of students with a closed group created on Facebook and through a questionnaire analyzed whether students use virtual environments for personal or educational. The survey reveals students perceptions about relevant aspects and the potential use of this tool as teaching-learning strategy

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The emergence of wavelength-division multiplexing (WDM) technology provides the capability for increasing the bandwidth of synchronous optical network (SONET) rings by grooming low-speed traffic streams onto different high-speed wavelength channels. Since the cost of SONET addâdrop multiplexers (SADM) at each node dominates the total cost of these networks, how to assign the wavelength, groom the traffic, and bypass the traffic through the intermediate nodes has received a lot of attention from researchers recently. Moreover, the traffic pattern of the optical network changes from time to time. How to develop dynamic reconfiguration algorithms for traffic grooming is an important issue. In this paper, two cases (best fit and full fit) for handling reconfigurable SONET over WDM networks are proposed. For each approach, an integer linear programming model and heuristic algorithms (TS-1 and TS-2, based on the tabu search method) are given. The results demonstrate that the TS-1 algorithm can yield better solutions but has a greater running time than the greedy algorithm for the best fit case. For the full fit case, the tabu search heuristic yields competitive results compared with an earlier simulated annealing based method and it is more stable for the dynamic case.

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The emergence of Wavelength Division Multiplexing (WDM) technology provides the capability for increasing the bandwidth of Synchronous Optical Network (SONET) rings by grooming low-speed traffic streams onto different high-speed wavelength channels. Since the cost of SONET add-drop multiplexers (SADM) at each node dominates the total cost of these networks, how to assign the wavelength, groom in the traffic and bypass the traffic through the intermediate nodes has received a lot of attention from researchers recently.

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Starting induction motors on isolated or weak systems is a highly dynamic process that can cause motor and load damage as well as electrical network fluctuations. Mechanical damage is associated with the high starting current drawn by a ramping induction motor. In order to compensate the load increase, the voltage of the electrical system decreases. Different starting methods can be applied to the electrical system to reduce these and other starting method issues. The purpose of this thesis is to build accurate and usable simulation models that can aid the designer in making the choice of an appropriate motor starting method. The specific case addressed is the situation where a diesel-generator set is used as the electrical supplied source to the induction motor. The most commonly used starting methods equivalent models are simulated and compared to each other. The main contributions of this thesis is that motor dynamic impedance is continuously calculated and fed back to the generator model to simulate the coupling of the electrical system. The comparative analysis given by the simulations has shown reasonably similar characteristics to other comparative studies. The diesel-generator and induction motor simulations have shown good results, and can adequately demonstrate the dynamics for testing and comparing the starting methods. Further work is suggested to refine the equivalent impedance presented in this thesis.

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Pollen and geochemical data from Little Lake, western Oregon, suggest several patterns of millennial-scale environmental change during marine isotope stage (MIS) 2 (14,100â27,600 cal yr B.P.) and the latter part of MIS 3 (27,600â42,500 cal yr B.P.). During MIS 3, a series of transitions between warm- and cold-adapted taxa indicate that temperatures oscillated by ca. 2±â4±C every 1000â3000 yr. Highs and lows in summer insolation during MIS 3 are generally associated with the warmest and coldest intervals. Warm periods at Little Lake correlate with warm sea-surface temperatures in the Santa Barbara Basin. Changes in the strength of the subtropical high and the jet stream may account for synchronous changes at the two sites. During MIS 2, shifts between mesic and xeric subalpine forests suggest changes in precipitation every 1000â3000 yr. Increases in Tsuga heterophylla pollen at 25,000 and 22,000 cal yr B.P. imply brief warmings. Minimum summer insolation and maximum global ice-volumes during MIS 2 correspond to cold and dry conditions. Fluctuations in precipitation at Little Lake do not correlate with changes in the Santa Barbara Basin and may be explained by variations in the strength of the glacial anticyclone and the position of the jet stream.

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The analysis of diatoms from two lake-sediment cores from southwestern Tasmania that span the Pleistocene-Holocene boundary provides insight about paleolimnological and paleoclimatic change in this region. Both Lake Vera (550 m elevation), in west-central Tasmania, and Eagle Tarn (1,033 m elevation), in south-central Tasmania, have lacustrine records that begin about 12,000 years ago. Despite significant differences in location, elevation, and geologic terrane, both lakes have, had similar, as well as synchronous, limnological histories. Each appears to have been larger and more alkaline 12,000 years ago than at present, and both became shallower through time. Fossil diatom assemblages about 11,500 years old indicate shallow-water environments that fluctuated in pH between acidic and alkaline, and between dilute and possibly slightly saline hydrochemical conditions ( The synchroneity and similar character of the paleolimnological changes at these separate and distinctive sites suggests a regional paleoclimatic cause rather than local environmental effects. Latest Pleistocene climates were apparently more continental and drier than Holocene climates in southwestern Tasmania.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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This paper deals with the emergence of explosive synchronization in scale-free networks by considering the Kuramoto model of coupled phase oscillators. The natural frequencies of oscillators are assumed to be correlated with their degrees, and a time delay is included in the system. This assumption allows enhancing the explosive transition to reach a synchronous state. We provide an analytical treatment developed in a star graph, which reproduces results obtained in scale-free networks. Our findings have important implications in understanding the synchronization of complex networks since the time delay is present in most real-world complex systems due to the finite speed of the signal transmission over a distance.

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In this paper, a modeling technique for small-signal stability assessment of unbalanced power systems is presented. Since power distribution systems are inherently unbalanced, due to its lines and loads characteristics, and the penetration of distributed generation into these systems is increasing nowadays, such a tool is needed in order to ensure a secure and reliable operation of these systems. The main contribution of this paper is the development of a phasor-based model for the study of dynamic phenomena in unbalanced power systems. Using an assumption on the net torque of the generator, it is possible to precisely define an equilibrium point for the phasor model of the system, thus enabling its linearization around this point, and, consequently, its eigenvalue/eigenvector analysis for small-signal stability assessment. The modeling technique presented here was compared to the dynamic behavior observed in ATP simulations and the results show that, for the generator and controller models used, the proposed modeling approach is adequate and yields reliable and precise results.

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Synchronous telecommunication networks, distributed control systems and integrated circuits have its accuracy of operation dependent on the existence of a reliable time basis signal extracted from the line data stream and acquirable to each node. In this sense, the existence of a sub-network (inside the main network) dedicated to the distribution of the clock signals is crucially important. There are different solutions for the architecture of the time distribution sub-network and choosing one of them depends on cost, precision, reliability and operational security. In this work we expose: (i) the possible time distribution networks and their usual topologies and arrangements. (ii) How parameters of the network nodes can affect the reachability and stability of the synchronous state of a network. (iii) Optimizations methods for synchronous networks which can provide low cost architectures with operational precision, reliability and security. (C) 2011 Elsevier B. V. All rights reserved.

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The design and implementation of a new control scheme for reactive power compensation, voltage regulation and transient stability enhancement for wind turbines equipped with fixed-speed induction generators (IGs) in large interconnected power systems is presented in this study. The low-voltage-ride-through (LVRT) capability is provided by extending the range of the operation of the controlled system to include typical post-fault conditions. A systematic procedure is proposed to design decentralised multi-variable controllers for large interconnected power systems using the linear quadratic (LQ) output-feedback control design method and the controller design procedure is formulated as an optimisation problem involving rank-constrained linear matrix inequality (LMI). In this study, it is shown that a static synchronous compensator (STATCOM) with energy storage system (ESS), controlled via robust control technique, is an effective device for improving the LVRT capability of fixed-speed wind turbines.