1000 resultados para produtividade da água e uniformidade de distribuição
Resumo:
Ser eficiente é um requisito para a sustentabilidade das empresas concessionárias de distribuição de energia elétrica no Brasil. A busca pela eficiência deve estar em harmonia com a melhoria contínua da qualidade, da segurança e da satisfação dos consumidores e das partes envolvidas. O desafio de atender múltiplos objetivos requer que as empresas do setor desenvolvam soluções inovadoras, com a mudança de processos, tecnologia, estrutura e a capacitação das pessoas. Desenvolver um modelo operacional eficiente e uma gestão rigorosa dos custos são fatores-chave para o sucesso das empresas, considerando o contexto regulatório de revisão tarifária que incentiva a melhoria do desempenho. O modelo operacional é definido a partir da organização logística dos recursos para atendimento da demanda de serviços, que define também os custos fixos e variáveis de pessoal (salário, horas extras, refeições), infraestrutura (manutenção de prédios, ferramentas e equipamentos) e deslocamentos (manutenção de veículos, combustível), por exemplo. A melhor alocação e o melhor dimensionamento de bases operacionais possibilitam a redução dos custos com deslocamento e infraestrutura, favorecendo o aproveitamento da força de trabalho em campo, a melhoria do atendimento dos clientes e da segurança dos colaboradores. Este trabalho apresenta uma metodologia de otimização de custos através da alocação de bases e equipes operacionais, com o modelamento matemático dos objetivos e restrições do negócio e a aplicação de algoritmo evolutivo para busca das melhores soluções, sendo uma aplicação de Pesquisa Operacional, no campo da Localização de Instalações, em distribuição de energia elétrica. O modelo de otimização desenvolvido possibilita a busca pelo ponto de equilíbrio ótimo que minimiza o custo total formado pelos custos de infraestrutura, frota (veículos e deslocamentos) e pessoal. O algoritmo evolutivo aplicado no modelo oferece soluções otimizadas pelo melhoramento de conjuntos de variáveis binárias com base em conceitos da evolução genética. O modelo de otimização fornece o detalhamento de toda a estrutura operacional e de custos para uma determinada solução do problema, utilizando premissas de produtividade e deslocamentos (velocidades e distâncias) para definir as abrangências de atuação das bases operacionais, recursos (equipes, pessoas, veículos) necessários para atendimento da demanda de serviços, e projetar todos os custos fixos e variáveis associados. A metodologia desenvolvida neste trabalho considera também a projeção de demanda futura para a aplicação no estudo de caso, que evidenciou a efetividade da metodologia como ferramenta para a melhoria da eficiência operacional em empresas de distribuição de energia elétrica.
Resumo:
O presente trabalho tem como base um estágio na entidade gestora INOVA-EM-S.A. a fim de reduzir a água não faturada. O estudo forcar-se-á especificamente nas perdas de água, às quais se dará uma maior importância às perdas reais. Este tema assume grande importância, notando-se cada vez mais, a consciência para este problema que afeta as entidades gestoras de todo o mundo. A nível nacional, a entidade reguladora - ERSAR define como principal alvo o valor de 20% de água não faturada, valor este que se tem revelado bastante inferior ao verificado para a realidade de várias entidades gestoras. Numa primeira fase, por forma a reduzir as perdas de água, será analisado todo o sistema de abastecimento de água do concelho de Cantanhede nomeadamente reservatórios, condutas, consumidores e os respetivos consumos. Posteriormente serão analisados todos os dados dos consumidores, bem como os das ZMCs presentes, resultando na elaboração de indicadores. Estes indicadores serão importantes para a tomada de decisão sobre qual ZMC intervir, e também aqui será essencial a análise dos caudais mínimos noturnos resultante do processo de telemetria. A escolha da zona de medição crítica será consequência dos valores referentes aos indicadores anteriormente referidos, assim como do tempo necessário para o estudo e todo o processo de atuação nesta ZMC. A ZMC (Bolho) escolhida será alvo de campanhas de deteção e intervenção, com a finalidade da redução das perdas reais, entretanto, paralelamente a este processo, será revisto todo o parque de contadores procedendo à verificação e mesmo à substituição de alguns contadores, combatendo assim parte das perdas aparentes. Estes processos serão acompanhados com a monitorização constante permitindo assim a verificação das medidas tomadas. Posteriormente serão abandonadas as intervenções, dando lugar a intervenções pontuais a realizar apenas quando necessário, mantendo a monitorização constante durante este período. Verificou-se que os indicadores, bem como os caudais mínimos noturnos subiram de forma considerável. Mais tarde será realizada uma nova campanha de deteção de fugas, desta vez não tão exaustiva como a anterior. Esta campanha será realizada com vista a analisar o estado da ZMC depois de realizado todo este processo exaustivo de intervenções. Como resultado desta segunda campanha serão identificados novos locais com roturas, locais que anteriormente na primeira campanha não foram destacados como locais a intervir por não apresentarem indícios de rotura ou fuga de água.
Resumo:
A utilização dos conceitos Lean de produção na logística é tida como um diferencial na criação de vantagem competitiva pelas empresas, inclusive por operadores logísticos. Na indústria automotiva, a prática de tais conceitos é utilizada de forma ampla. O conceito de se ter um sistema enxuto de fornecimento pode levar ao simples entendimento de restrição de fornecedores e redução dos níveis de estoque através do aumento de freqüência das entregas, contudo esta é uma visão distorcida quando falamos de sistema enxuto, pois o sistema enxuto trata da análise de eliminação de desperdício de todo o sistema envolvido em um processo. O objetivo desta dissertação foi de realizar um estudo exploratório sobre os aspectos determinantes do sucesso na implantação do conceito de logística lean e sua aplicação na logística de distribuição de peças de uma empresa automotiva, através de um operador logístico. A metodologia utilizada para a neste trabalho é uma revisão bibliográfica e a imersão em um ambiente de operação logística terceirizada em um depósito de uma indústria automotiva de distribuição de peças à rede autorizada, onde o autor atua como agente para melhoria dos processos operacionais in loco. Como resultado foi observado um aumento na produtividade das operações, bem como uma redução de área utilizada e do tempo de resposta dos pedidos colocados.(AU)
Resumo:
The Monte Carlo method is accurate and is relatively simple to implement for the solution of problems involving complex geometries and anisotropic scattering of radiation as compared with other numerical techniques. In addition, differently of what happens for most of numerical techniques, for which the associated simulations computational time tends to increase exponentially with the complexity of the problems, in the Monte Carlo the increase of the computational time tends to be linear. Nevertheless, the Monte Carlo solution is highly computer time consuming for most of the interest problems. The Multispectral Energy Bundle model allows the reduction of the computational time associated to the Monte Carlo solution. The referred model is here analyzed for applications in media constituted for nonparticipating species and water vapor, which is an important emitting species formed during the combustion of hydrocarbon fuels. Aspects related to computer time optimization are investigated the model solutions are compared with benchmark line-by-line solutions
Resumo:
On the evening of April 1, 1981, the city of Santa Cruz was taken by a flood that destroyed the spillway weir Santa Cruz and much of the city, causing the population of the lower areas to abandon their homes. Residences and closest to the dam, the river and the creek Trairi Sin streets were totally affected, and many of the houses and public buildings completely destroyed. Located in the state of Rio Grande do Norte, the city of Santa Cruz is marked by a very hot climate and semi-arid. With an average normal rainfall of 498.7 mm per year, the city is affected by drought since the beginning of its foundation, around the year 1831. For its development had continued, it was necessary that the population actualize various environmental interventions Nature, especially as regards the storage and distribution of water. Considering the process of occupying the space in question, as well as the relationships that human groups have with the natural environment, we aim to analyze how human interventions affect the environment around them, modifying it to meet their needs and interests. Thus, we intend to understand how media coverage enabled the construction of a framework of tragedy. We intend to observe how the occupation of Santa Cruz led to the modification of their surroundings, trying to discuss the effects of human intervention on natural disasters. Among the research sources include newspapers A República, Diário de Natal, Tribuna do Norte, O Poti and Folha de São Paulo, as well as interviews with the then mayor of Santa Cruz, Hildebrando Teixeira de Souza, and with the couple José Henrique de Pontes and Severina Aguida de Pontes, whose house was hit by the flood. We will also take messages from the state government, the minutes of the city council of Santa Cruz and reports produced by DNOCS on the construction of the weir and its survey after the flood.
Lâminas de água salina e doses de adubação orgânica na produção de palma Miúda adensada no semiárido
Resumo:
Cactus pear is an important forage for livestock in semi-arid region of Brazil, due to its adaptation to climate conditions in this region, high productivity and nutritional value. The yield of this cactus has positively responded to techniques such as planting density, fertilization and cutting managements, however, in Rio Grande do Norte State, only certain areas have favorable climate conditions to the development of this crop. Drip irrigation, with a small amount of water, has proven to be an alternative to the viability of cactus pear cultivation in these areas. The research aimed to evaluate the effects of different levels of saline water and manure organic fertilization on the morphological characteristics and production of fresh and dry matter of the prickly-pear cactus cv. Miúda (Nopalea cochenillifera Salm Dick) in a dense planting system. The experiment was conducted at the Experimental Station of Terras Secas (EMPARN), Pedro Avelino, latitude 5°31'21" South and longitude 36°23'14" West. The soil was classified as Typical Cambisol Haplicum Carbonate and the water used in irrigation, C4S1T3 (5,25 dS.m-1), with planting spacing of 2.0 x 0.25 m (20,000 plants ha-1). A completely randomized design in a split plot was used, where water levels (0, 7.5, 15.0 and 30.0 mm month-1) with 10 days intervals, were the main plots and organic fertilization (0 , 25 and 50 Mg ha-1 yr-1) the subplots, with four replicates. The measured morphological characteristics were number of cladodes, height and volume of the plant; length, width, perimeter, thickness, area and cladodes area index, fresh and dry matter production, dry matter content, water use efficiency (WUE) and damage promoted by cochineal pest (Diaspis echinocacti) and soft rot (Erwinia carotovora). There was no influence (P>0.05) of organic fertilization on most variables, particularly in relation to the production of fresh and dry matter. The water levels had a significant influence (P<0.05) on most variables, promoting higher height and volume of the plants, larger and thicker cladodes, and increase on fresh and dry matter production (13.55 Mg DM ha-1 yr-1). The absence of irrigation caused a significant expansion in plant damage caused by the cochineal pest and when irrigated with different water levels there was an increase in damage and stand loss, caused by soft rot, been more intense at the higher water level.
Resumo:
The stabilization of energy supply in Brazil has been a challenge for the operation of the National Interconnected System in face of hydrological and climatic variations. Thermoelectric plants have been used as an emergency source for periods of water scarcity. The utilization of fossil fuels, however, has elevated the cost of electricity. On the other hand, offshore wind energy has gained importance in the international context and is competitive enough to become a possibility for future generation in Brazil. In this scenario, the main goal of this thesis was to investigate the magnitude and distribution of offshore wind resources, and also verify the possibilities of complementing hydropower. A data series of precipitation from the Climatic Research Unit (CRU) Blended Sea Winds from the National Climatic Data Center (NCDC/NOAA) were used. According to statistical criteria, three types of complementarity were found in the Brazilian territory: hydro × hydro, wind × wind and hydro × wind. It was noted a significant complementarity between wind and hydro resources (r = -0.65), mainly for the hydrographical basins of the southeast and central regions with Northeastern Brazil winds. To refine the extrapolation of winds over the ocean, a method based on the Monin-Obukhov theory was used to model the stability of the atmospheric boundary layer. Objectively Analyzed Air-Sea Flux (OAFLUX) datasets for heat flux, temperature and humidity, and also sea level pressure data from NCEP/NCAR were used. The ETOPO1 from the National Geophysical Data Center (NGDC/NOAA) provided bathymetric data. It was found that shallow waters, between 0-20 meters, have a resource estimated at 559 GW. The contribution of wind resources to hydroelectric reservoir operation was investigated with a simplified hybrid wind-hydraulic model, and reservoir level, inflow, outflow and turbine production data. It was found that the hybrid system avoids drought periods, continuously saving water from reservoirs through wind production. Therefore, from the results obtained, it is possible to state that the good winds from the Brazilian coast can, besides diversifying the electric matrix, stabilize the hydrological fluctuations avoiding rationing and blackouts, reducing the use of thermal power plants, increasing the production cost and emission of greenhouse gases. Public policies targeted to offshore wind energy will be necessary for its full development.
Resumo:
Currently, the management recommendations for asian soybean rust (ASR) has been based on the application of protective fungicides mixed with triazoles and stronilurins. Thus, this study aimed at assessing whether the increased productivity provided by the application of protective fungicides is due solely to the fungicidal action of the product or some physiological changes in the plant and which the latter would be. The experiment was conducted from March to July 2015 at the experimental station of Udi Research and Development in Uberlândia-MG, with the cultivar 97Y07 RR. The experimental design chosen for this study was comprised of a randomized block with four replications and 16 treatments: check, fluxapyroxad + pyraclostrobin (116.55 + 58.45 g ha-1), azoxystrobin + benzovindiflupir (90 + 45 g ha-1), trifloxystrobin + prothioconazole (60 + 70 g ha-1), tebuconazole + picoxystrobin (100 + 60 g ha-1), picoxystrobin + cyproconazole (60 + 24 g ha-1), mancozeb (1125 g ha-1), azoxistrobina + tebuconazole + difenoconazole (60 + 75 + 120 g ha-1), azoxystrobin + tebuconazole + difenoconazole + chlorothalonil ( 60 + 120 + 75 + 1440 g ha-1), and mistures fluxapyroxad + pyraclostrobin + mancozeb, azoxystrobin + benzovindiflupir + mancozeb, trifloxystrobin + prothioconazole + mancozeb, tebuconazole + picoxystrobin + mancozeb, picoxystrobin + cyproconazole + mancozeb, azoxystrobin + tebuconazole + difenoconazole + mancozeb, and azoxystrobin + benzovindiflupir + chlorothalonil, from the aforesaid doses. The first application of the treatments occurred in R1, in the absence of symptoms. The number of applications, intervals and the use of adjuvants were performed according to the recommendations by manufacturers. The variables analyzed were: disease severity, concentration of chlorophylls and carotenoids, photosynthetic rate (A), transpiration rate (E), stomatal conductance (gs), internal carbon concentration (Ci), instantaneous efficiency in water use (A/E), intrinsic water use efficiency (A/gs), and carboxylation efficiency (A/C). With these data collected, this study set to date the progress curve of each variable (AUPC). At the end of the crop cycle, the average of pods per plant was quantified, grain per pod, productivity and weight of 1,000 grains. It was concluded that: the addition of mancozeb to fluxapyroxad + pyraclostrobin, azoxystrobin + benzovindiflupir, trifloxystrobin + prothioconazole and tebuconazole + picoxystrobin potentiated the ASR control; adding mancozebe to the mixture azoxystrobin + benzovindiflupir provided better control of the disease compared to the addition of chlorothalonil; mancozeb amounts to AUPC concentration of photosynthetic pigments and when added to axozystrobin + tebuconazole + difenoconazole, increases the AUPC for total chlorophyll concentration, as well as when chlorothalonil was added; mancozeb added to the mix fluxapyroxad + pyraclostrobin raised the AUPC for A/Ci and A/gs, increasing the W1,000G and crop productivity; the addition of protectors similarly reflected on the productivity of culture.
Resumo:
A água é um recurso essencial e escasso, como tal, é necessário encontrar medidas que permitam o seu uso de modo sustentável e garantam a proteção do meio ambiente. Devido a esta crescente preocupação assiste-se a um movimento legislativo, nacional e internacional, no sentido de garantir o desenvolvimento sustentável. Surge assim, a Diretiva Quadro da Água e a Lei da Água, que é complementada com legislação diversa. Como elemento constituinte do ciclo urbano da água, os Sistemas de Abastecimento têm sofrido evoluções nem sempre adequadas. É neste contexto que, em Portugal, nascem as diversas ferramentas para a melhoria da gestão dos recursos hídricos. As Entidades Gestoras têm como finalidade a gestão eficiente do bem água, e dispõe de dois importantes instrumentos, o Programa Nacional para o Uso Eficiente da Água e o Guia para o “controlo de perdas de água em sistemas públicos de adução e distribuição”(ERSAR). Esta Gestão passa, não só pela abordagem da problemática das perdas de água, reais e aparentes, como também pela análise do comportamento que origina o desperdício. A APA, enquanto entidade gestora, procura maximizar a eficiência do seu sistema de abastecimento, para tal, foram aplicadas as ferramentas propostas pelo ERSAR. Concluindo-se que este sistema tem um total de perdas de água de 34%, devendo-se estas perdas essencialmente ao envelhecido parque de contadores e perdas nos ramais de distribuição (teórico). As perdas comerciais representam cerca de 69%, o que revela que os volumes de água não faturados (medidos ou não) são muito elevados. Por outro lado, a realização do cálculo do Balanço Hídrico e dos índices de desempenho permitem classificar a performance do sistema de abastecimento e compará-la com os seus objetivos de gestão. Atendendo ao volume de água perdido nos ramais, foram efetuadas medições noturnas, verificando-se que no Porto de Pesca Costeira existe um volume de água escoado não justificado. Neste sentido, elaborou-se um plano de ação para aumentar a eficiência do sistema, ou seja, reduzir as perdas totais de 34% para 15%.
Resumo:
In order to add value to soybens crops, and hence the marketing, medium and large producers have been using precision agriculture techniques (PA), as the Remote Sensing, Geographic Information Systems (GIS) and positioning satellite, to assist the management of crops. Thus, given the economic relevance of that culture to the southwest of Paraná State and Brazil, scientific studies to increase their productivity and profitability are of main importance. The objective of this study was to evaluate the correlation between the chemical soil properties and soybean yield for each estimated parameter of semivariogram (range, nugget and level effect), and the deployment of these correlations in direct and indirect effects, aiming to improve the mapping process of spatial variability of soil chemical properties for use in PA. The hypothesis is that not all attributes of soil used to estimate the semivariogram parameters has a direct effect on productivity, and that even in groups of plants within a larger area it is possible to estimate the parameters of the semivariograms. The experiment was conducted in a commercial area of 19.7 ha, located in the city of Pato Branco - PR, central geographic coordinates 26º 11 '35 "South, 52 43' 05" West longitude, and average altitude of 780 m. The area is planted with soybeans for over 30 years, currently being adopted to cultivate Brasmax Target RR - Don Mario 5.9i, with row spacing of 0.50 m and 13 plants m-1, totaling 260,000 plants ha-1. For georeferencing of the area of study and sampling points was used a couple of topographic ProMarkTM3 receptors, making a relative positioning to obtain the georeferenced coordinates. To collect data (chemical analyzes of soil and crop yield) were sampled 10 blocks in the experimental area, each with an area of 20 m2 (20 meters long x 1 meter wide) containing two spaced adjacent rows of 0.5 m. Each block was divided into 20 portions of 1 m2, and from each were collected four subsamples at a distance of 0.5 m in relation to the lines of blocks, making up a sample depth for 0-10 cm a sample to 10-20 cm for each plot, totaling 200 samples for each depth. The soybean crop was performed on the blocks depending on maturity, and in each block was considered a bundle at each meter. In the data analysis, it was performed a diagnosis of multicollinearity, and subsequently a path analysis of the main variables according to the explanatory variables (range of chemical attributes: pH, K, P, Ca, etc.). The results obtained by the path analysis of the parameters of the semivariogram of soil chemical properties, indicated that only the Fe, Mg, Mn, organic matter (OM), P and Saturation by bases (SB) exerted direct and indirect effects on soybean productivity, although they have not presented spatial variability, indicating that the distribution of blocks in the area was unable to identify the spatial dependence of these elements, making it impossible to draw up maps of the chemical attributes for use in PA.
Resumo:
The main issues related to water conservation in urban centers are the increase in water supply cost, demand growth, pollution and differences in the distribution of water resources. Water conservation, the controlled and efficient use of water, includes both measures as reasonable means of water reuse. Thus, conservation practices are an effective way to meet demand and supply water to new activities and users without jeopardizing the supplying water bodies and preserving the natural environment. This study aims to examine the water management of a shopping mall and the use of rainwater harvesting combined with greywater reuse. For buildings in general, water loss is common due to leaks in the hydraulic and restroom equipment. These losses, which are caused by a high volume of water used and wasted in the system, are often the result of design errors, incorrect maintenance procedures and users' bad habits In southern Brazil, where there is rainfall almost all year long, water shortages occasionally occur, particularly in some winter mouths. One difficulty that appears on rainwater studies is the proper determination of rainwater volume that can be used to address water supply systems. In this work, the simulation method was used to determine this volume. Thus, simulations with the following variables: rainfall, catchment area and water consumption were performed. For mall's hydraulic systems, segmented alternatives are adopted. That is, focusing on the use of rainwater or greywater reuse. Other alternatives of effluent reuse have been slightly discussed due to sanitary issues, those are effluents from toilets and kitchen sinks. The adoption of greywater may be feasible if there is a significant flow of greywater to comply water demand for toilet flushing. The inspections made in this study found that the quantity of sinks was insufficient to supply an adequate amount of water to toilets and urinals. The greywater reuse system was found to be infeasible in terms of demand and supply of water. Conversely, the rainwater harvesting system was entirely feasible and easily supplied water to all restrooms and contributed to the cooling of the air conditioning system with a short payback period. One of the challenges of this work was the need to compare the actual water consumption with a water consumption parameter used in buildings. Thus, a method that addresses the generation of specific consumption indexes for specific activity (like a mall) was used. The water consumption indices showed that this mall has a satisfactory water management program.
Resumo:
Os Planos de Segurança da Água surgem com a necessidade de aumentar a segurança da água de abastecimento, superando a monitorização de conformidade de “fim de linha”, permitindo aumentar a confiança do consumidor na qualidade da água que lhe é fornecida. Esta nova abordagem recorre a uma metodologia de gestão baseada na identificação e no controlo de riscos em pontos críticos de um sistema de abastecimento, em complemento do controlo realizado através da monitorização da conformidade da água entregue aos consumidores. O Plano de Segurança da Água (PSA) encontra-se implementado no Sistema Regional do Carvoeiro (SRC) desde o ano de 2009. O SRC é um sistema de abastecimento de água em alta, sendo constituído por conjunto de infraestruturas de captação, tratamento, transporte e armazenamento de água desde a sua origem, localizada no rio Vouga, em Carvoeiro, até aos municípios integrados na Associação de Municípios do Carvoeiro-Vouga. Atendendo à obra de expansão do SRC, tornou-se imperativo efetuar uma revisão ao PSA, sendo este o objetivo primordial do trabalho de estágio desenvolvido na empresa Águas do Vouga S.A, concessionária responsável pela gestão do SRC. Para a prossecução deste objetivo, o trabalho desenvolvido envolveu os seguintes passos metodológicos: identificação das operações aplicadas no SRC; identificação de perigos e eventos perigosos em todos os órgãos constituintes do sistema; avaliação de riscos; identificação de pontos críticos de controlo; identificação de pontos de monitorização e medidas preventivas; elaboração do plano de monitorização, incluindo, procedimentos de controlo operacional em condições normais de funcionamento e em caso de desvio; validação deste plano. Deste trabalho resultou a identificação de 166 eventos perigosos, 17 tipologias de perigos, 3 pontos de controlo crítico e 17 pontos de monitorização. Os pontos de controlo crítico foram identificados nos processos de tratamento da ETA do Carvoeiro. O primeiro foi localizado na etapa de filtração com areia, antracite e zeólitos correspondendo aos perigos com metais (Fe e Mn), outros compostos químicos perigosos, partículas, turvação, matéria orgânica e alumínio. O segundo ponto foi identificado na etapa de filtração com filtros de carvão ativado granular relativo ao aparecimento de sabor e cianotoxinas. O terceiro ponto de controlo crítico foi encontrado na etapa de desinfeção referente aos microrganismos patogénicos. Os pontos de monitorização foram localizados ao longo do sistema em situações onde não se dispõem de nenhuma medida de controlo para eliminar o perigo e antes e após os pontos de controlo crítico. O plano de monitorização foi desenvolvido para estes pontos, embora os limites e procedimentos definidos devam ser alvo de revisão após a conclusão da obra de expansão do sistema. A validação da revisão do plano foi iniciada, mas cingiu-se apenas na avaliação preliminar de riscos, prévia ao início de operação da ETA do Carvoeiro. Para além da revisão deste plano, foram realizadas outras tarefas, nomeadamente uma análise à qualidade da água fornecida e distribuída pelo sistema, a elaboração do plano PCQA para o ano de 2016, a configuração da plataforma de gestão operacional NAVIATM e a revisão do Manual de Gestão da Águas do Vouga relativo ao processo de qualidade na captação, tratamento e distribuição e ao processo de qualidade na gestão do PSA.
Resumo:
Guaraíras lagoon, located in Tibau do Sul in the eastern littoral of Rio Grande do Norte (Brazil), presents a permanent connection to the sea, which guarantees the occurrence of a rich biodiversity, which includes the autochthonous shrimp species Litopenaeus schmitti, Farfantepenaeus subtilis and Farfantepenaeus brasiliensis. In spite of being subject to a strong human intervention in the last decade, mainly related to the installation of shrimp (Litopenaeus vannamei) farms, the lagoon is still scarcely studied. The present study aims at characterizing the populations of the three autochthonous penaeid shrimp species inhabiting Guaraíras, taking into consideration their abundance and seasonal distribution in the inflow channel of Primar System of Organic Aquaculture (Tibau do Sul, Rio Grande do Norte, Brazil). Twelve monthly samples were carried out from May 2005 to April 2006 with the aid of a circular cast net in the inflow channel, which is daily supplied with water from Guaraíras. Sampling months were grouped in trimesters according to the total pluviosity, thus comprising four trimesters. Water salinity was monitored twice a week and temperature values registered on a daily basis at noon, during the study period. The daily pluviosity data from the municipality of Tibau do Sul were supplied by Empresa de Pesquisa Agropecuária do Rio Grande do Norte (EMPARN). Collected shrimp were identified, weighted, measured and sexed. L. schmitti specimens (0.2 g to 17.8 g) were distributed in 1.3 g weight classes intervals. From the eighth sampling month (December 2005) onwards, males were classified into three categories, in accordance with the development of their petasm: (a) rudimentary petasm, (b) partially formed petasm, and (c) completely formed petasm. Among the ecological variables, rainfall showed the greatest dispersion (s.d.=187.74Rainfall and abundance of L. schmitti were negatively correlated (r = -0.85) whereas its abundance and water salinity were positively correlated (r = 0.63). Among 1,144 collected individuals, 1,127 were L. schmitti, 13 were F. subtilis and 4 were F. brasiliensis, which corresponded to 98.51%, 1.14% and 0.35% of the total of collected individuals. L. schmitti occurred in 100 % of all samples. Differently, the presence of F. subtilis and F. brasiliensis was restricted to 33% and 17% of the collected samples, respectively. The present study confirmed the occurrence of L. schmitti, F. brasiliensis and F. subtilis in Guaraíras. However, this lagoon seems to be primarily inhabited by juvenile Litopenaeus schmitti. The population of L. schmitti analysed showed a seasonal pattern of distribution. In general, in the months of high salinity and absence of rain, the number of individuals was higher than in the wet months. Further studies on the reproductive biology and ecology of L. schmitti, F. brasiliensis and F. subtilis may elucidate questions referring to the abundance, period, and phase of occurrence of these shrimp genera in Guaraíras. Finally, the risks associated to the establishment of L. vannamei in the lagoon provide a novel outlet for studies in this biotope
Resumo:
Dissertação (mestrado)—Universidade de Brasília, Faculdade Gama, Programa de Pós-Graduação em Engenharia Biomédica, 2015.
Resumo:
Seaweeds sulfated polysaccharides have been described as having various pharmacological activities. However, nothing is known about the influence of salinity on the structure of sulfated polysaccharides from green seaweed and pharmacological activities they perform. Therefore, the main aim of this study was to evaluate the effect of salinity of seawater on yield and composition of polysaccharides-rich fractions from green seaweed Caulerpa cupressoides var. flabellata, collected in two different salinities beaches of the coast of Rio Grande do Norte, and to verify the influence of salinity on their biological activities. We extracted four sulfated polysaccharides-rich fractions from C. cupressoides collected in Camapum beach (denominated CCM F0.3; F0.5; F1.0; F2.0), which the seawater has higher salinity, and Buzios beach (denominated CCB F0.3; F0.5; F1.0; F2.0). Different from that observed for other seaweeds, the proximate composition of C. cupressoides did not change with increased salinity. Moreover, interestingly, the C. cupresoides have high amounts of protein, greater even than other edible seaweeds. There was no significant difference (p>0.05) between the yield of polysaccharide fractions of CCM and its CCB counterparts, which indicates that salinity does not interfere with the yield of polysaccharide fractions. However, there was a significant difference in the sulfate/sugar ratio of F0.3 (p<0.05) and F0.5 (p<0.01) (CCM F0.3 and CCB F0.5 was higher than those determined for their counterparts), while the sulfate/sugar ratio the F1.0 and F2.0 did not change significantly (p>0.05) with salinity. This result suggested that the observed difference in the sulfate/sugar ratio between the fractions from CCM and CCB, is not merely a function of salinity, but probably also is related to the biological function of these biopolymers in seaweed. In addition, the salinity variation between collection sites did not influence algal monosaccharide composition, eletrophoretic mobility or the infrared spectrum of polysaccharides, demonstrating that the salinity does not change the composition of sulfated polysaccharides of C. cupressoides. There were differences in antioxidant and anticoagulant fractions between CCM and CCB. CCB F0.3 (more sulfated) had higher total antioxidant capacity that CCM F0.3, since the chelating ability the CCM F0.5 was more potent than CCB F0.5 (more sulfated). These data indicate that the activities of sulfated polysaccharides from CCM and CCB depend on the spatial patterns of sulfate groups and that it is unlikely to be merely a charge density effect. C. cupressoides polysaccharides also exhibited anticoagulant activity in the intrinsic (aPTT test) and extrinsic pathway (PT test). CCB F1.0 and CCM F1.0 showed different (p<0,001) aPTT activity, although F0.3 and F0.5 showed no difference (p>0,05) between CCM and CCB, corroborating the fact that the sulfate/sugar ratio is not a determining factor for biological activity, but rather for sulfate distribution along the sugar chain. Moreover, F0.3 and F0.5 activity in aPTT test was similar to that of clexane®, anticoagulant drug. In addition, F0.5 showed PT activity. These results suggest that salinity may have created subtle differences in the structure of sulfated polysaccharides, such as the distribution of sulfate groups, which would cause differences in biological activities between the fractions of the CCM and the CCB