Desenvolvimento de biossensor eletroquímico para diagnóstico de dengue

Pós-graduação em Biotecnologia - IQ

Otimização de procedimentos de controle de qualidade em equipamentos radiográficos convencionais do Instituto de Eletrotécnica e Energia - USP

Radiography is currently an important method of diagnosis, both medical, dental and veterinary. The image in this type of survey is obtained using an X-ray beam, where the radiologist can possibly view structures of interest. It isn't always possible to get the desired images due to various factors, such as equipment limitations. The Administrative Law 453/98, the State Resolution SS 625/94, and other standards require testing and quality control acceptance limits that guarantee a good performance of the equipment for the security and quality of care, giving service users greater effectiveness in exams. This study were performed in the accompaniment of testing procedures for quality control established by Administrative Law 453/98 in several X-ray equipment to make a comparison and optimization in the descriptions of the procedures used by the Institute for Electrical Energy and the University of São Paulo (IEE / USP / SP).The optimization of the procedures were performed with the aid of a current literature, the Resolution 453/98, State Resolution SS 625/94 and other international standards. On this basis it was possible to observe the importance of regular monitoring of tests for an update, following the technological development of instruments used in the service

A luta em defesa da Sociologia no Ensino Médio: 1996-2007 : um estudo sobre a invenção das tradições

Pós-graduação em Educação Escolar - FCLAR

Descrição e análise de contingências presentes em legislações referentes à mobilidade urbana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Extrinsic factors affecting water absorption in chilling of broiler chicken carcasses

Brazil occupies an outstanding position as a producer and exporter of chicken meat, and the maintenance and expansion of this position require a constant evolution, especially in variables which determine quality. An important quality parameter of poultry meat is the amount of water absorbed by the carcass during processing. In Brazil, carcasses chilling is done by immersion in chilled water. In this process, the carcass is rehydrated and the water lost during transport and initial operations is replaced. At this stage, some care is needed to prevent the absorption of water upper than the level allowed by Brazilian law. This project aimed to evaluate extrinsic factors that can influence the absorption of water by the chicken meat. For this, 144 Cobb chickens divided into 24 groups of six birds were used. At 42 days of age, one chicken of each group, with weight ranging up to 10% more or less from the average of the group, was slaughtered in an experimental pilot scale abattoir where slaughter procedures were conducted under strictly controlled conditions. The chilling procedure was performed following a completely randomized design with factorial arrangement 3x2, where the factors were: three temperatures in the first section of the chilling system (4, 10 and 16ºC) and two degrees of water hardness (hard and soft water), with six treatments and four replications. Brazilian law provides that the water temperature in the first section of the chiller must not be higher than 16ºC, and the length of the carcasses in this section shall not exceed 30 minutes. All carcasses remained in the first section of the chiller for 30 minutes and then were transferred to another tank with water at 4ºC, remaining there until reaching 7ºC. The carcasses were weighed before and after chilling, to evaluate the percentage of water absorbed. The water absorption was influenced by the initial temperature of the water in the chiller and by the water hardness. When initially immersed in water at 4ºC, carcasses water absorption averaged 2.70%, a significantly lower absorption than the values found for the carcasses that were initially immersed in water at 16ºC, 3.83% (p<0.05). The carcasses immersed in water at 10ºC had mean water absorption of 3.66%, not differing from the means observed in the other two treatments (p>0.05). In hard water, the average water absorption was 2.46% and, in soft water, 4.33% (p<0.05). In all treatments, the water absorption did not exceed the limit established by Brazilian legislation, which is a maximum of 8%. This information is important to control the absorption of water by carcasses in chicken meat processing, preventing consumers from being harmed.

Função afim e suas propriedades através da resolução de problemas

Pós-graduação em Matemática em Rede Nacional - IBILCE

Condições de acessibilidade na universidade: o ponto de vista de estudantes com deficiência

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Systematics of interstitial encrusting bryozoans from southeastern Brazil

This paper describes 22 species of marine bryozoans found in the sand-grain-encrusting interstitial epifauna of the northeast coast of São Paulo state, Brazil: one new cyclostome, Disporella calcitrapa sp. nov., and 21 cheilostomes. Sixteen of the cheilostomes are new species, and three represent new genera. They are Ammatophora arenacea sp. nov., Discoporella gemmulifera sp. nov., Puellina caraguata sp. nov., Puellina tuba sp. nov., Rosulapelta rosetta gen. et sp. nov., Collarina spicata sp. nov., Hippothoa calcicola sp. nov., Trypostega ilhabelae sp. nov., Reptadeonella granulosa sp. nov., Drepanophora irregularis sp. nov., Allotherenia sabulosa gen. et sp. nov., Bryopesanser tilbrooki sp. nov., Psammocleidochasma tridentatum gen. et sp. nov., Celleporina abstrusa sp. nov., Hippoporella castellana sp. nov., and Hippoporella sabulonis sp. nov. Other species found in this habitat, Alderina smitti, Cymulopora uniserialis, Vibracellina laxibasis, Akatopora leucocypha, and Smittipora sawayai, have previously been described. The family Cymuloporidae fam. nov. is erected for Cymulopora and Crepis. The occurrence in this habitat of living colonies of bryozoans more characteristic of larger subtidal shell substrata indicates the potential importance of an interstitial refuge in maintaining and dispersing encrusting bryozoan populations along continental shelves where larger substrata are absent or rare.

Sintesi e caratterizzazione di nanoparticelle di ossido di stagno dopate

In the last years, new materials have been developed in the broad area of nanoscience. Among them, an emergent class characterized by excellent electrical conductivity properties as well as high optical transmittance in the visible region are TCOs (Transparent Conducting Oxides). Due to their versatile properties, they have found many applications in a lot of optoelectronic devices, such as solar cells, liquid crystal displays, touch-panel displays, gas sensors, to cite a few examples. Different research groups have studied and characterized the TCOs. In this context, a new synthetic method has been developed to produce FTO nanocrystals (Fluorine-doped Tin Oxide NCs) in Prof. Pinna’s lab at the Humboldt University in Berlin. FTO belongs to the TCO category, and they have been studied as a promising alternative to ITO NCs (Indium Tin Oxide) which represent the standard TCO material in terms of properties and performances. In this work, FTO NCs have been synthesized using the “benzyl alcohol route” (a non-aqueous sol-gel method) via microwave, which permits to produce FTO particles with good properties as revealed by the characterizations performed, employing a cheap, fast and clean method.

Organotypic slice cultures from rat brain tissue: a new approach for Naegleria fowleri CNS infection in vitro

The free-living amoeba Naegleria fowleri is the aetiological agent of primary amoebic meningoencephalitis (PAM), a disease leading to death in the vast majority of cases. In patients suffering from PAM, and in corresponding animal models, the brain undergoes a massive inflammatory response, followed by haemorrhage and severe tissue necrosis. Both, in vivo and in vitro models are currently being used to study PAM infection. However, animal models may pose ethical issues, are dependent upon availability of specific infrastructural facilities, and are time-consuming and costly. Conversely, cell cultures lack the complex organ-specific morphology found in vivo, and thus, findings obtained in vitro do not necessarily reflect the situation in vivo. The present study reports infection of organotypic slice cultures from rat brain with N. fowleri and compares the findings in this culture system with in vivo infection in a rat model of PAM, that proved complementary to that of mice. We found that brain morphology, as present in vivo, is well retained in organotypic slice cultures, and that infection time-course including tissue damage parallels the observations in vivo in the rat. Therefore, organotypic slice cultures from rat brain offer a new in vitro approach to study N. fowleri infection in the context of PAM.

Geochemistry of ODP Hole 121-752A

The present study involves the analysis and interpretation of geochemical data from a suite of sediment samples recovered at ODP Hole 752A. The samples encompass the time period that includes the lithospheric extension and uplift of Broken Ridge, and they record deposition below and above the mid-Eocene angular unconformity that denotes this uplift. A Q-mode factor analysis of the geochemical data indicates that the sediments in this section are composed of a mixture of three geochemical end members that collectively account for 94.2% of the total variance in the data. An examination of interelement ratios for each of these end members suggests that they represent the following sedimentary components: (1) a biogenic component, (2) a volcanogenic component, and (3) a hydrothermal component. The flux of the biogenic component decreases almost thirtyfold across the Eocene unconformity. This drastic reduction in the deposition of biogenic materials corresponds to the almost complete disappearance of chert layers, diatoms, and siliceous microfossils and is coincident with the uplift of Broken Ridge. The volcanogenic component is similar in composition to Santonian ash recovered at Hole 755A on Broken Ridge and is the apparent source of the Fe-stained sediment that immediately overlies the angular unconformity. This finding suggests that significant amounts of Santonian ash were subaerially exposed, weathered, and redeposited and is consistent with data that suggest that the vertical uplift of Broken Ridge was both rapid and extensive. The greatest flux of hydrothermal materials is recorded in the sediments immediately below the angular unconformity. This implies that the uplift of Broken Ridge was preceded by a significant amount of rifting, during which faulting and fracturing of the lithosphere led to enhanced hydrothermal circulation. This time sequence of events is consistent with (but not necessarily diagnostic of) the passive model of lithospheric extension and uplift.

Bóvedas y contrarresto del Colegio de Nuestra Señora de la Antigua de Monforte de Lemos : geometría, construcción y mecánica

El Colegio de Nuestra Señora de la Antigua de Monforte de Lemos (1593-1619) constituye el principal ejemplo del clasicismo herreriano de Galicia. Esta tesis analiza los aspectos técnicos de la construcción de sus bóvedas de cantería, en el marco disciplinar de la Historia de la Construcción. El Colegio contiene varios tipos de bóvedas entre las que destacan su cúpula trasdosada y su escalera monumental. Además, se conservan los documentos notariales históricos que describen las obras y una de las monteas más importantes de Galicia. Las bóvedas han sufrido movimientos y algunas presentan importantes deformaciones, que han suscitado preocupación sobre su estabilidad. El estudio se organiza en tres epígrafes: geometría, construcción y mecánica, tres aspectos estrechamente interrelacionados en este tipo de estructuras. La parte de estudio geométrico parte de un levantamiento en el que se emplea una estación total sin reflectante, una técnica que permite una medición muy precisa de la forma real de las bóvedas. Como complemento se ha recurrido a técnicas de escáner fotográfico. Con los datos de las mediciones se han elaborado los dibujos de las bóvedas, y analizado sus medidas e irregularidades. Se ha incluido un análisis metrológico por métodos inductivos, que ha puesto de manifiesto la unidad utilizada en la construcción. El estudio se completa con la recopilación de las reglas de dimensionamiento que figuran en los tratados, que se comparan con las dimensiones de las bóvedas analizadas. En el estudio constructivo se registran los despieces y la configuración constructiva y se contrastan, por un lado, con las soluciones que aparecen en los principales tratados de los siglos XVI, XVII y XVIII, y por otro, con las descripciones que se encuentran en los documentos históricos del Colegio. Se ha prestado especial atención al estudio de estos documentos, que han permitido conocer algunos aspectos originales del proceso de construcción de estas bóvedas. En una de las paredes del claustro se ha identificado una montea inédita cuyo levantamiento y análisis se incorpora en esta parte. En el estudio mecánico se analiza la estabilidad dentro del marco teórico del Moderno Análisis Límite de Estructuras de Fábricas. La comprensión del comportamiento estructural, unida a la precisión de los levantamientos, ha permitido explicar los movimientos de las fábricas e inferir la geometría original de algunas de las bóvedas, no siempre evidente en los casos de fuertes deformaciones. Los estudios realizados hasta ahora en bóvedas de los siglos XVI y XVII permiten vislumbrar un panorama de soluciones constructivas más rico que el que recogen los tratados históricos. Las nuevas técnicas de medición sin contacto abren nuevas posibilidades para analizar estas estructuras. Los casos estudiados todavía son escasos y se necesita un conjunto más amplio para poder realizar un análisis general. Esta tesis aporta el estudio de uno de esos casos con el fin de contribuir al mejor conocimiento de la construcción en España en los siglos XVI y XVII. ABSTRACT The Colegio de Nuestra Señora de la Antigua (1593-1619), in Monforte de Lemos, Spain, is the best example in Galicia of the classicism influenced by the Monastery of El Escorial and his architect, Juan de Herrera. This thesis analyzes the technical aspects of the construction of its masonry vaults within the discipline of Construction History. The Colegio exhibits various types of vaults, among which the extradosed ashlar dome and the grand staircase are particularly worth mentioning. In addition, the legal documents containing thorough specifications of the work, as well as one of the construction drawings at actual size —one of the best examples in Galicia—, have both been preserved to this date. Some of the vaults have undergone important deformations that have raised concerns about their stability. The study is organized in three sections: geometry, construction, and mechanics, the three clearly interrelated in this type of structures. The geometrical study starts out with a metric survey using a reflectorless total station, a technique that allows a very precise measurement of the actual shape of the vault. This technique was complemented with the use of a photo-based 3D scanner. The resulting measurements were used to draw the vaults, and analyze their dimensions and irregularities. An inductive metrological analysis, which was able to reveal the exact metric unit utilized during the construction, is included in this section. The section is completed with the gathering of the dimensional rules appearing in the various historical treatises, which are compared with the dimensions of the actual vaults. The construction study deals with the quartering of the structure, whose results are compared, on the one hand, with the approaches appearing in the main treatises of the 16th- 18th centuries, and on the other, with the descriptions in the historical documents of the Colegio itself. Special attention has been paid to the study of these documents, which have revealed some original aspects in the construction process of the vaults. In one of the cloister’s walls, a hitherto unheard-of construction drawing at actual size was found. Its survey and analysis is included in this section. The mechanical study analyzes the Colegio's vaults stability within the theoretical frame of the Modern Limit Analysis of Masonry Structures. The interpretation of the structural behavior, coupled with the precision of the surveys, has allowed a deep understanding of the masonry’s movements and the original geometry of some of the vaults —not always evident in the case of strong deformations— has been inferred. The studies dealing with XVI and XVII century vaults conducted up to this date show a landscape of constructive solutions far richer than the one suggested in the historical treatises. The new contactless measuring techniques offer exciting possibilities for the analysis of these structures. The cases studied are still few and more would be needed before a general analysis can be attempted. This thesis constitutes one such additional case and its goal is to improve our understanding of Spanish construction in the 16th-17th centuries.

Impacto de las redes sociales digitales en la innovación y la ventaja competitiva : un estudio de casos

Internet y las Tecnologías de la Información y las Comunicaciones han transformado de manera notable la forma de relacionarse de las personas. Actualmente, la presencia de estas nuevas tecnologías está totalmente asumida e impacta en el día a día de millones de personas en todo el mundo. Dentro de este contexto se presentan las Redes Sociales Digitales como plataformas que han dado lugar a un cambio de mentalidad, creando una nueva forma de comprender y utilizar internet. En este proyecto se ha llevado a cabo un estudio del fenómeno de las redes sociales dentro del mundo empresarial con el fin especificar su viabilidad como herramienta profesional dentro del sector de las TIC en términos de innovación, ventaja competitiva, y riesgo. El desarrollo de este trabajo se ha abarcado en dos partes principales: en primer lugar, un análisis teórico a cerca de las ventajas competitivas que puede ofrecer esta tecnología y cómo se puede traducir en valor real para una empresa. Y en segundo lugar un estudio de casos práctico donde se ha estudiado la experiencia de distintas empresas en el uso del Social Media. Previo a la implementación de estos puntos, este estudio se ha enmarcado dentro del mundo de las TIC, presentando conceptos como la web 2.0, los tipos herramientas que pueden ser aplicadas por la empresa y modelos de negocio que se presentan en Internet. La finalidad de este trabajo consiste en presentar qué factores de los expuestos de manera teórica generan ventaja competitiva y beneficio o por el contrario, desventaja y riesgo a la empresa, apoyándose en las conclusiones obtenidas a través del estudio de casos. ABSTRACT Internet and Information and Communication Technologies have remarkably transformed the personal relationship. Thus, these technologies are completely assumed and affect to the daily life of millions of people. Under this context, is presented the Digital Social Media as platforms which have changed the global mindset triggering a new way of understanding and using the Internet. In this project, has been carried out a study of the Social Media within the professional TIC world, aimed to specify its viability as working tool in terms of innovation, competitive advantage and risk. The implementation has been covered in two main parts: First, a theoretical analysis about the competitive advantage which this technology could offer and how it can become into real value for a company. And secondly, a Case Study in order to analyze different company’s experiences in the use of Social Media and providing empirical support to the obtained conclusions in the theoretical part. In addition, before develop these two points, the project has been integrated in the TIC´s field, meeting concepts as Web 2.0, different kinds of internet-based tools which can be used by a company and business models based on the information technologies.

A divulgação de provisões e passivos contingentes ambientais pelas empresas do setor de energia elétrica em comparação ao desenvolvimento da normatização da evidenciação contábil

O pressuposto desta pesquisa é de que a divulgação de informações ambientais, no âmbito das provisões e passivos contingentes, reagiu aos avanços na normatização contábil. A normatização contábil genérica sobre evidenciação de obrigações incertas era restrita, em meados de 1976, à Lei no 6.404, e assim permaneceu ao longo de pelo menos uma década e meia, quando começou a ser desenvolvida. Ao longo dos anos foram criados padrões obrigatórios de divulgação, com critérios de julgamento mais detalhados para a classificação da obrigação incerta em provável, possível ou remota. Embora ainda apresente algum grau de subjetividade, o desenvolvimento destes critérios pode ter contribuído para a diminuição da assimetria informacional: a empresa passou a contar com um conjunto de orientações mais claras e, portanto, com melhores condições de averiguar e divulgar suas obrigações incertas. Esse avanço contribuiu para que as obrigações ambientais passassem a ter maior exposição, principalmente no âmbito das empresas potencialmente poluidoras, como as do setor de energia elétrica, que utilizam recursos naturais e modificam o meio ambiente. Neste contexto, o objetivo deste estudo foi analisar as evidências de passivo ambiental divulgadas pelas empresas do setor de energia elétrica, de 1997 a 2014. Para tanto, foi desenvolvido um estudo qualitativo, descritivo e longitudinal, por meio da análise de conteúdo de 941 notas explicativas, de uma população de 64 empresas do setor de energia elétrica, de acordo com listagem na BM&FBovespa, em maio de 2015. A amostra foi constituída de 26 empresas, que divulgaram o total de 468 notas explicativas no site da CVM, de 1997 a 2014. Ao longo destes 18 anos, 14 empresas da amostra (53,85%) evidenciaram passivos ambientais ao menos uma vez e 12 instituições (46,15%) não o fizeram e, do total de 468 notas explicativas, 100 (21,37%) evidenciaram passivo ambiental. O número de evidências de passivos ambientais era pequeno em meados de 1997, mas ascendeu, com um aumento mais consistente a partir de 2006, ano que coincide com a aprovação da Norma e Procedimento de Contabilidade 22 - Provisões, Passivos, Contingências Passivas e Contingências Ativas, emitida pelo IBRACON. Adicionalmente, a materialidade quantitativa estava na média de 0,61% para provisões ambientais e 0,89% para os passivos contingentes ambientais, desconsiderando-se os outliers. A dimensão das notas explicativas, em termos de quantidade de palavras, foi crescente e diversificada. Em conclusão, a evidenciação contábil pode, em adição à evidenciação voluntária, ser um meio plausível para a divulgação de questões ambientais e redução da assimetria informacional, principalmente quando a normatização contábil se faz mais clara e detalhada.

CIC-5: A chloride channel with multiple roles in renal tubular albumin uptake

CIC-5 is a chloride (Cl-) channel expressed in renal tubules and is critical for normal tubular function. Loss of function nonsense or missense mutations in CIC-5 are associated with Dent's disease, a condition in which patients present with low molecular weight (LMW) proteinuria (including albuminuria), hypercalciuria and nephrolithiasis. Several key studies in CIC-5 knockout mice have shown that the proteinuria results from defective tubular reabsorption of proteins. CIC-5 is typically regarded as an intracellular Cl- channel and thus the defect in this receptor-mediated uptake pathway was initially attributed to the failure of the early endosomes to acidify correctly. CIC-5 was postulated to play a key role in transporting the Cl- ions required to compensate for the movement of H+ during endosomal acidification. However, more recent studies suggest additional roles for CIC-5 in the endocytosis of albumin. CIC-5 is now known to be expressed at low levels at the cell surface and appears to be a key component in the assembly of the macromolecular complex involved in protein endocytosis. Furthermore, mutations in CIC-5 affect the trafficking of v-H+-ATPase and result in decreased expression of the albumin receptor megalin/cubulin. Thus, the expression of CIC-5 at the cell surface as well as its presence in endosomes appears to be essential for normal protein uptake by the renal proximal tubule. (c) 2005 Elsevier Ltd. All rights reserved.