Batuque: reverberação da memória na vidência de identidades afro-amazônicas

Esta dissertação é o produto de um estudo analítico do livro de poemas Batuque, de Bruno de Menezes, com foco nas narrativas de memória. Tomam-se como referência as manifestações culturais de origem africana representadas no livro, em consonância com teorias pautadas sob uma ótica direcionada à conexão entre a subjetividade literária e a objetividade histórica, tendo como referência os conceitos de História Social, propostos por Peter Burke, a ideia de cultura de Terry Eagleton e a identidade cultural, de Stuart Hall. Esses instrumentos possibilitam a observação da presença africana como elemento de composição de uma identidade cultural na Amazônia, sob três aspectos: o primeiro, relacionado ao lirismo, demonstrado, sobretudo, nos poemas “Mãe Preta” e “Pai João”; o segundo, direcionado à musicalidade, observada em poemas como “Batuque” e “Alma e ritmo da raça”; e o terceiro versa sobre a religiosidade, apresentada em poemas como “Toiá Verequête” e “Oração da Cabra Preta”. Destes três aspectos, o que mais despertou nossa atenção foi a religiosidade, em especial quanto à relação entre sagrado e profano e à visão de bem e mal, muito evidentes na obra. Os resultados obtidos dão-nos a ideia de que, pela leitura das narrativas de memória do autor e de sua relação com o ambiente em que viveu, as influências do meio na criação artística do poeta e na produção de uma obra voltada para as manifestações culturais contribuem, significativamente, para a melhor compreensão da presença negra na cultura brasileira.

As repercussões da interiorização da UFPA no trabalho dos docentes da rede estadual de ensino nas décadas de 1980 e 1990

O estudo teve por objetivo analisar o modelo brasileiro de expansão da educação superior (ES) a partir das reformas e políticas públicas destinadas a essa educação, ocorridas no Brasil, com atenção às décadas de 1980 e 1990, a fim de compreender a dinâmica do processo de instalação, materialização e consolidação da interiorização da UFPA, no período do estudo, com vista a identificar e desvelar possíveis repercussões dessa experiência no trabalho dos docentes da Rede Estadual de Ensino no Estado do Pará. A expansão da ES é tema central e as repercussões da Interiorização da UFPA, no trabalho dos docentes da Rede de Educação Pública no Pará, o objeto amplo de estudo. O lócus da investigação foi o Campus Universitário da UFPA instalado no Município de Santarém, na Região Oeste do Pará, na Amazônia Brasileira. Buscou-se saber: como se apresentava a expansão da ES pública no Brasil no período do estudo? Quais eram os seus condicionantes? Quais fatores contribuíram para a UFPA tornar o acesso à ES pública realidade no Pará? Quais as repercussões da Interiorização da UFPA no trabalho dos docentes da Rede Estadual de Ensino? O estudo envolveu pesquisa bibliográfica e empírica, seu caráter é teórico-analítico exploratório e os pressupostos teórico-metodológicos se pautaram no materialismo histórico dialético. Para capturar, analisar e desvelar os indicadores que dão materialidade ao objeto de estudo, procurou-se articular o conhecimento teórico já produzido com o conteúdo de documentos oficiais e os dados coletados a partir dos depoimentos concedidos pelos quinze sujeitos entrevistados. A coleta de dados abrangeu Santarém, Óbidos, Alenquer e Itaituba, primeiros Núcleos vinculados ao Campus. O estudo mostrou que há uma estreita relação entre as mudanças ocorridas no mundo capitalista e as reformas políticas públicas, educacionais e trabalhistas, no país, influenciando a Educação Brasileira, o trabalho docente e a decisão da UFPA em expandir e consolidar suas atividades no interior do Pará, desde a experiência inicial. Em função dos condicionantes estruturais, a Interiorização da UFPA recebeu muitas críticas pertinentes, em função do modelo adotado no país que impôs e interpôs limitações à ES Brasileira e seu processo de expansão/interiorização, contudo, como mostram os depoimentos, repercutiu favoravelmente no trabalho dos docentes da Rede Estadual de Ensino no Oeste Paraense, nas décadas de 1980 e 1990, em relação à formação em nível de graduação; aquisição de novos conhecimentos; definição de uma nova postura profissional; melhorias salariais; possibilidade de ascensão de profissionais da educação para outros níveis de ensino; além de representar condição inicial para instalação e consolidação da oferta de ES (pública e privada) na região. A própria UFPA foi criada na primeira experiência formal de interiorização ocorrida no país, decretada pelo presidente Getúlio Vargas, sob a influência da Reforma Francisco Campos, na década de 1930. A expansão de sua atuação para o interior ocorreu a partir de acordos locais, regionais, nacionais e internacionais firmados a partir do final da década de 1960, como parte das políticas de expansão da ditadura militar ocorrida no país (1964-1985).

A reconstrução histórica da cabanagem em “Lealdade” e da guerra civil moçambicana em “As Duas sombras do rio”

Nesta tese, pretendemos analisar comparativamente a reconstrução histórica da Cabanagem e da Guerra Civil Moçambicana nos romances Lealdade (1997), de Márcio Souza e As duas sombras do rio (2003), de João Paulo Borges Coelho. Para tanto, apresentaremos um breve percurso histórico da colonização brasileira e moçambicana, bem como o período da independência e pós-independência, além do percurso teórico sobre o romance histórico, resistência, memória, bem como a teoria sobre o espaço, nesse caso o rio, que utilizamos como ferramenta de análise. Utilizando o rio como fio condutor de nossa análise. Na obra de Borges Coelho, a análise foi feita a partir das travessias das personagens pelos rios que foram desencadeadas pela chegada da guerra civil. Fixamos nossa leitura em Leónidas Ntsato personagem que metaforiza Moçambique dividido em dois pela guerra civil e destacamos o papel do narrador neste romance. Na narrativa de Márcio Souza acompanhamos as viagens de Fernando, narrador do romance, que tem sua biografia entrelaçada aos acontecimentos que desencadearão a Cabanagem anos mais tarde. Cada um com seu estilo, os dois romancistas revisitam as agruras das duas guerras que tem como palco o Norte do Brasil e de Moçambique que são espaços periféricos desde os tempos coloniais.

Localização da proteína fosfolipase C zeta em extratos esppermáticos de gatos domésticos normospérmicos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo semiótico sobre a verbovisualidade em manuais de língua inglesa

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

As fontes do método analítico de leitura de João Köpke (1896-1917)

After two decades advertising and defending the synthetic method, João Köpke became active disseminator of analytical method for teaching reading. In five te xts published between 1896 and 1917, produced in different circumstance s for different publics readers, Köpke seeks the support of American and European authors, for their principles as well as for the education experience, to his analytical method. as by theirs principles as by theirs educational experiences. Among those authors: J. Jacotot, A. Bain, A. Meiklejohn, J. Froebel, C. Parker, G. Stanley Hall and J. Chubb. In this article, attention is given to repeated American references with special emphasis on the psychology of Stanley Hall and the method of teaching reading of Meiklejohn, highlighted and placed on convergence by Köpke in his final writings.

Concilier les données qualitatives et quantitatives: un défi pour les recherches en acquisition du langage

What is the role of qualitative data? How should mixed approaches be considered? Is it necessary to complement the quantitative approach by the qualitative approach (Maziére et al., 2010) or the opposite? How can these two approaches be combined in a Language Acquisition research? What are the possibilities of generalization that these approaches provide? Besides those necessary theoretical discussions, our starting point is a qualitative research on the acquisition of the plural morpheme in Brazilian Portuguese (BP) in children between 1 year 11 months old and 2 years 7 months old (Hilário, 2012), whose linguistic production is intrinsically connected to their dialogic aspect (Bakhtin & Vološinov, 1973) and to the interaction among interlocutors (Bruner, 2004; Vygotsky, 1986). In this research through the usage of a computational tool (CLAN) it is expected to have more visibility of the quantitative results even if they are in a qualitative approach. Although, it should be taken into account the fact that each approach can achieve valid and relevant results (Valsiner 2000), i.e., with any given method it is possible to reach a kind of generalization (statistical or analytical).

Relações de trabalho dos jogadores profissionais do poker: um estudo à luz da psicossociologia

Pós-graduação em Psicologia - FCLAS

É, não sou: ensaios sobre os afro-sambas no tempo e no espaço

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O estilo em psicanálise: o discurso do analista como arte do bem dizer

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Septin C-Terminal Domain Interactions: Implications for Filament Stability and Assembly

Septins form a conserved family of filament forming GTP binding proteins found in a wide range of eukaryotic cells. They share a common structural architecture consisting of an N-terminal domain, a central GTP binding domain and a C-terminal domain, which is often predicted to adopt a coiled-coil conformation, at least in part. The crystal structure of the human SEPT2/SEPT6/SEPT7 heterocomplex has revealed the importance of the GTP binding domain in filament formation, but surprisingly no electron density was observed for the C-terminal domains and their function remains obscure. The dearth of structural information concerning the C-terminal region has motivated the present study in which the putative C-terminal domains of human SEPT2, SEPT6 and SEPT7 were expressed in E. coli and purified to homogeneity. The thermal stability and secondary structure content of the domains were studied by circular dichroism spectroscopy, and homo- and hetero-interactions were investigated by size exclusion chromatography, chemical cross-linking, analytical ultracentrifugation and surface plasmon resonance. Our results show that SEPT6-C and SEPT7-C are able to form both homo- and heterodimers with a high alpha-helical content in solution. The heterodimer is elongated and considerably more stable than the homodimers, with a K (D) of 15.8 nM. On the other hand, the homodimer SEPT2-C has a much lower affinity, with a K (D) of 4 mu M, and a moderate alpha-helical content. Our findings present the first direct experimental evidence toward better understanding the biophysical properties and coiled-coil pairings of such domains and their potential role in filament assembly and stability.

Low resolution structural characterization of the Hsp70-interacting protein - Hip - from Leishmania braziliensis emphasizes its high asymmetry

The Hsp70 is an essential molecular chaperone in protein metabolism since it acts as a pivot with other molecular chaperone families. Several co-chaperones act as regulators of the Hsp70 action cycle, as for instance Hip (Hsp70-interacting protein). Hip is a tetratricopeptide repeat protein (TPR) that interacts with the ATPase domain in the Hsp70-ADP state, stabilizing it and preventing substrate dissociation. Molecular chaperones from protozoans, which can cause some neglected diseases, are poorly studied in terms of structure and function. Here, we investigated the structural features of Hip from the protozoa Leishmania braziliensis (LbHip), one of the causative agents of the leishmaniasis disease. LbHip was heterologously expressed and purified in the folded state, as attested by circular dichroism and intrinsic fluorescence emission techniques. LbHip forms an elongated dimer, as observed by analytical gel filtration chromatography, analytical ultracentrifugation and small angle X-ray scattering (SAXS). With the SAXS data a low resolution model was reconstructed, which shed light on the structure of this protein, emphasizing its elongated shape and suggesting its domain organization. We also investigated the chemical-induced unfolding behavior of LbHip and two transitions were observed. The first transition was related to the unfolding of the TPR domain of each protomer and the second transition of the dimer dissociation. Altogether. LbHip presents a similar structure to mammalian Hip, despite their low level of conservation, suggesting that this class of eukaryotic protein may use a similar mechanism of action. (C) 2012 Elsevier Inc. All rights reserved.

Identification of Regions Involved in Substrate Binding and Dimer Stabilization within the Central Domains of Yeast Hsp40 Sis1

Protein folding, refolding and degradation are essential for cellular life and are regulated by protein homeostatic processes such those that involve the molecular chaperone DnaK/Hsp70 and its co-chaperone DnaJ. Hsp70 action is initiated when proteins from the DnaJ family bind an unfolded protein for delivery purposes. In eukaryotes, the DnaJ family can be divided into two main groups, Type I and Type II, represented by yeast cytosolic Ydj1 and Sis1, respectively. Although sharing some unique features both members of the DnaJ family, Ydj1 and Sis1 are structurally and functionally distinct as deemed by previous studies, including the observation that their central domains carry the structural and functional information even in switched chimeras. In this study, we combined several biophysical tools for evaluating the stability of Sis1 and mutants that had the central domains (named Gly/Met rich domain and C-terminal Domain I) deleted or switched to those of Ydj1 to gain insight into the role of these regions in the structure and function of Sis1. The mutants retained some functions similar to full length wild-type Sis1, however they were defective in others. We found that: 1) Sis1 unfolds in at least two steps as follows: folded dimer to partially folded monomer and then to an unfolded monomer. 2) The Gly/Met rich domain had intrinsically disordered characteristics and its deletion had no effect on the conformational stability of the protein. 3) The deletion of the C-terminal Domain I perturbed the stability of the dimer. 4) Exchanging the central domains perturbed the conformational stability of the protein. Altogether, our results suggest the existence of two similar subdomains in the C-terminal domain of DnaJ that could be important for stabilizing each other in order to maintain a folded substrate-binding site as well as the dimeric state of the protein.

Platelet-derived exosomes induce endothelial cell apoptosis through peroxynitrite generation: experimental evidence for a novel mechanism of septic vascular dysfunction

Abstract Introduction Several studies link hematological dysfunction to severity of sepsis. Previously we showed that platelet-derived microparticles from septic patients induce vascular cell apoptosis through the NADPH oxidase-dependent release of superoxide. We sought to further characterize the microparticle-dependent vascular injury pathway. Methods During septic shock there is increased generation of thrombin, TNF-α and nitric oxide (NO). Human platelets were exposed for 1 hour to the NO donor diethylamine-NONOate (0.5 μM), lipopolysaccharide (LPS; 100 ng/ml), TNF-α (40 ng/ml), or thrombin (5 IU/ml). Microparticles were recovered through filtration and ultracentrifugation and analyzed by electron microscopy, flow cytometry or Western blotting for protein identification. Redox activity was characterized by lucigenin (5 μM) or coelenterazine (5 μM) luminescence and by 4,5-diaminofluorescein (10 mM) and 2',7'-dichlorofluorescein (10 mM) fluorescence. Endothelial cell apoptosis was detected by phosphatidylserine exposure and by measurement of caspase-3 activity with an enzyme-linked immunoassay. Results Size, morphology, high exposure of the tetraspanins CD9, CD63, and CD81, together with low phosphatidylserine, showed that platelets exposed to NONOate and LPS, but not to TNF-α or thrombin, generate microparticles similar to those recovered from septic patients, and characterize them as exosomes. Luminescence and fluorescence studies, and the use of specific inhibitors, revealed concomitant superoxide and NO generation. Western blots showed the presence of NO synthase II (but not isoforms I or III) and of the NADPH oxidase subunits p22phox, protein disulfide isomerase and Nox. Endothelial cells exposed to the exosomes underwent apoptosis and caspase-3 activation, which were inhibited by NO synthase inhibitors or by a superoxide dismutase mimetic and totally blocked by urate (1 mM), suggesting a role for the peroxynitrite radical. None of these redox properties and proapoptotic effects was evident in microparticles recovered from platelets exposed to thrombin or TNF-α. Conclusion We showed that, in sepsis, NO and bacterial elements are responsible for type-specific platelet-derived exosome generation. Those exosomes have an active role in vascular signaling as redox-active particles that can induce endothelial cell caspase-3 activation and apoptosis by generating superoxide, NO and peroxynitrite. Thus, exosomes must be considered for further developments in understanding and treating vascular dysfunction in sepsis.

The unconventional secretion of stress-inducible protein 1 by a heterogeneous population of extracellular vesicles

The co-chaperone stress-inducible protein 1 (STI1) is released by astrocytes, and has important neurotrophic properties upon binding to prion protein (PrPC). However, STI1 lacks a signal peptide and pharmacological approaches pointed that it does not follow a classical secretion mechanism. Ultracentrifugation, size exclusion chromatography, electron microscopy, vesicle labeling, and particle tracking analysis were used to identify three major types of extracellular vesicles (EVs) released from astrocytes with sizes ranging from 20–50, 100–200, and 300–400 nm. These EVs carry STI1 and present many exosomal markers, even though only a subpopulation had the typical exosomal morphology. The only protein, from those evaluated here, present exclusively in vesicles that have exosomal morphology was PrPC. STI1 partially co-localized with Rab5 and Rab7 in endosomal compartments, and a dominant-negative for vacuolar protein sorting 4A (VPS4A), required for formation of multivesicular bodies (MVBs), impaired EV and STI1 release. Flow cytometry and PK digestion demonstrated that STI1 localized to the outer leaflet of EVs, and its association with EVs greatly increased STI1 activity upon PrPC-dependent neuronal signaling. These results indicate that astrocytes secrete a diverse population of EVs derived from MVBs that contain STI1 and suggest that the interaction between EVs and neuronal surface components enhances STI1–PrPC signaling