Dinámicas biopoéticas del "Prototractatus" y el "Tractatus Logico-Philosophicus" de Ludwig Wittgenstein : arquitectura cognitiva de las proposiciones

Programa de doctorado: Nuevas perspectivas cognitivas en los estudios de lengua, literatura y traducción. La fecha de publicación es la fecha de lectura Tesis doctoral europea

Kiel, Ewald / Kahlert, Joachim / Haag, Ludwig / Eberle, Thomas (Hrsg.): Herausfordernde Situationen in der Schule, Ein fallbasiertes Arbeitsbuch. Bad Heilbrunn: Klinkhardt 2011 [...] [Sammelrezension]

Sammelrezension von: 1. Kiel, Ewald / Kahlert, Joachim / Haag, Ludwig / Eberle, Thomas (Hrsg.): Herausfordernde Situationen in der Schule, Ein fallbasiertes Arbeitsbuch, Bad Heilbrunn: Klinkhardt 2011 (224 S.; ISBN 978-3-7815-1799-8) 2. Kiel, Ewald / Pollak, Guido (Hrsg.): Kritische Situationen im Referendariat bewältigen, Ein Arbeitsbuch für angehende Lehrkräfte, Bad Heilbrunn: Klinkhardt 2011 (271 S.; ISBN 978-3-8252-3544-4)

Ludwig Fertig: Vor-Leben. Bekenntnis und Erziehung bei Thomas Mann. Darmstadt: Wissenschaftliche Buchgesellschaft 1994 [Rezension]

Rezension von: Ludwig Fertig: Vor-Leben. Bekenntnis und Erziehung bei Thomas Mann. Darmstadt: Wissenschaftliche Buchgesellschaft 1994, 312 S.

Ludwig Liegle: Frühpädagogik. Erziehung und Bildung kleiner Kinder. Ein dialogischer Ansatz. Stuttgart: Kohlhammer 2013 (172 S.) [Rezension]

Rezension von: Ludwig Liegle: Frühpädagogik. Erziehung und Bildung kleiner Kinder. Ein dialogischer Ansatz. Stuttgart: Kohlhammer 2013 (172 S.; ISBN 978-3-17-022480-3)

Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries

Background: Head and neck squamous cell carcinoma (HNSCC) is one of the most common malignancies in humans. The average 5-year survival rate is one of the lowest among aggressive cancers, showing no significant improvement in recent years. When detected early, HNSCC has a good prognosis, but most patients present metastatic disease at the time of diagnosis, which significantly reduces survival rate. Despite extensive research, no molecular markers are currently available for diagnostic or prognostic purposes. Methods: Aiming to identify differentially-expressed genes involved in laryngeal squamous cell carcinoma (LSCC) development and progression, we generated individual Serial Analysis of Gene Expression (SAGE) libraries from a metastatic and non-metastatic larynx carcinoma, as well as from a normal larynx mucosa sample. Approximately 54,000 unique tags were sequenced in three libraries. Results: Statistical data analysis identified a subset of 1,216 differentially expressed tags between tumor and normal libraries, and 894 differentially expressed tags between metastatic and non-metastatic carcinomas. Three genes displaying differential regulation, one down-regulated (KRT31) and two up-regulated (BST2, MFAP2), as well as one with a non-significant differential expression pattern (GNA15) in our SAGE data were selected for real-time polymerase chain reaction (PCR) in a set of HNSCC samples. Consistent with our statistical analysis, quantitative PCR confirmed the upregulation of BST2 and MFAP2 and the downregulation of KRT31 when samples of HNSCC were compared to tumor-free surgical margins. As expected, GNA15 presented a non-significant differential expression pattern when tumor samples were compared to normal tissues. Conclusion: To the best of our knowledge, this is the first study reporting SAGE data in head and neck squamous cell tumors. Statistical analysis was effective in identifying differentially expressed genes reportedly involved in cancer development. The differential expression of a subset of genes was confirmed in additional larynx carcinoma samples and in carcinomas from a distinct head and neck subsite. This result suggests the existence of potential common biomarkers for prognosis and targeted-therapy development in this heterogeneous type of tumor.

Genomics and proteomics approaches to the study of cancer-stroma interactions

Background: The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression. Methods: The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells. Results: We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR. Conclusions: A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein.

A score system for quality evaluation of RNA sequence tags: an improvement for gene expression profiling

Background: High-throughput molecular approaches for gene expression profiling, such as Serial Analysis of Gene Expression (SAGE), Massively Parallel Signature Sequencing (MPSS) or Sequencing-by-Synthesis (SBS) represent powerful techniques that provide global transcription profiles of different cell types through sequencing of short fragments of transcripts, denominated sequence tags. These techniques have improved our understanding about the relationships between these expression profiles and cellular phenotypes. Despite this, more reliable datasets are still necessary. In this work, we present a web-based tool named S3T: Score System for Sequence Tags, to index sequenced tags in accordance with their reliability. This is made through a series of evaluations based on a defined rule set. S3T allows the identification/selection of tags, considered more reliable for further gene expression analysis. Results: This methodology was applied to a public SAGE dataset. In order to compare data before and after filtering, a hierarchical clustering analysis was performed in samples from the same type of tissue, in distinct biological conditions, using these two datasets. Our results provide evidences suggesting that it is possible to find more congruous clusters after using S3T scoring system. Conclusion: These results substantiate the proposed application to generate more reliable data. This is a significant contribution for determination of global gene expression profiles. The library analysis with S3T is freely available at http://gdm.fmrp.usp.br/s3t/.S3T source code and datasets can also be downloaded from the aforementioned website.