Cell Death in Salivary Glands of Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Acari: Ixodidae) Females at Semi-engorged Feeding Stage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

"Espaços (mal) ditos": representações dos bordéis Mossoroenses nas décadas de 1950 e 1960

The city that presents itself to our eyes is not just made of stone. The urban layout hides the tactics of the subjects who subjectivate spaces, giving it meaning through the representations that it produces, whether written or oral. The city's art, is represented, is home to the place where the good and bad places. The aim of this study is to analyze the representations about the spaces occupied by mossoroenses brothels in the 1950 and 1960. With this goal we organize the work into three chapters. At first, we present the formation of red-light district in the city, highlighting the composition of the bohemian spaces in good and bad places. In the second chapter we discuss how the representations developed by the newspaper O Mossoroense created a space damn for identifying the area as a place of crime and misdemeanor. In the last chapter we seek to identify and reconstruct the residents of Mossoró, in contemporary times, their memories about the peak time of the brothels in the city. In oral narratives seek to understand how people describe the places of the brothel, comparing the spatiality of the past with this experienced in the city. Therefore, we discuss how these spaces have been turned into places of pleasure and a curse, as places become common in places marked morally.

No tempo dos brotos: juventude e diversão em Petrópolis e no Tirol (1945-1960)

The urban growth of Rio Grande do Norte capital gave his boldest step in the three earlier decades of the twentieth century seeking to catch up with foreign metropolis and also most developed Brazilian cities, for example, Rio de Janeiro. Novelties were such bulky that we can notice the rise of a new city which in many aspects superseded that one considered provincial by local intelligentsia. These urban interferences scratched city ground leaving indelible marks until the present day. The exacerbated growth of the city related to the 1940s, when Natal begins hosting the largest allied military base outside United States in the advent of World War II is important at a time when the city´s population doubled. The emergence of new leisure institutions, amidst already existing ones, continued marking the places as sociabilities and leisure permeated with meanings and representations are revealed. This work sought to know the sociability of Tirol and Petrópolis neighborhoods in Natal, in the period from 1945 to 1960, when the city witnesses the consolidation of a market increasingly focused on leisure and young people start to have a special participation in this process.

O Bairro da Ribeira como um palimpsesto: dinâmicas urbanas na Cidade de Natal (1920-1960)

As cidades são textos. Textos escritos pelas sociedades que compõe os espaços. A partir dessa perspectiva, propõe-se aqui uma leitura do bairro da Ribeira Natal/RN. Esse espaço se consolidou no início do século XX como centro comercial e cultural da capital potiguar, lugar de ação e de interação da Zona Central desta sociedade, espaço de inscrição dos signos da modernidade e do progresso então em voga. Contudo, com o decurso do tempo e as transformações por ele exigidas, o panorama da Cidade de Natal sofreu intensas modificações e o bairro da Ribeira foi relegado à margem desse processo, o que culminou na desvalorização, segregação e, posteriormente, na degradação do seu espaço, que se tornou um palimpsesto. Ao abordar a dinâmica urbana que se processou no bairro da Ribeira, esta pesquisa dividiu o fenômeno em três fases distintas: valorização, transição e decadência

Feira livre de Macaíba/RN : um estudo das modificações na dinâmica socioespacial (1960/2006)

The objective of the current piece of research is to reflect upon the diverse changes that have occurred in the social and spatial dynamics of the Macaíba fair in the period between 1960 and 2006. During the second half of the 19th century, Macaíba had in the commerce one of this principle economic base a contribution for which the city became one of the main commercial warehouses of the East coast of the Rio Grande do Norte region. This helped lead to the growth of Macaíba s fair, which proved to be one of the most important existing in the state until the 1970 s. In the last two decades of the 20th century, certain elements represented challenges to the fair at Macaíba. These challenges stimulated substantial changes in the fair s dynamics which include the growth and expansion of the commercial and service sector, primarily though supermarkets; consolidation among the commercial and distribution networks, represented by the Central Office of Supply of the Rio Grande do Norte S/A (Ceasa/RN), by the wholesale and refrigeration companies; and the modernization of transportation methods, which permitted an expanded reach for these networks. Even with all these changes, the fair continues to be one of the strongest aspects of the city being the center of resistance against the surge of new forms of commerce and consumption in the city (notable the supermarkets) and the diffusion of other aspects of globalization. The fair has economic importance, as it offers a popular marketplace for the commercialization of very different products and a means for supplying goods to the residents of the city and the rural communities of Macaíba and the surrounding municipalities; and socio-cultural importance in that the fair is a place where popular tradition is expressed, a place where a great number of parallel activities occur, a place for meeting again and again, of conversations, of manifestations of culture and art, and of socialization in all of its dimensions

Sobre pedras, entre rios: modernização do espaço urbano de Caicó/RN (1950/1960)

thèse de maîtrise, qui a été idealizée et exécutée à partir de la réunion et les relations dialogiques établies entre la géographie et l'histoire, et l'espace et le temps, a considéré l'environnement urbain comme le thème général et, comme la référence empirique, l'espace urbain de Caicó, incrusté dans les terres semi-arides du Sertão do Seridó Potiguar, plus précisément à la mi-sud de l'État du Rio Grande do Norte. Dans cet espace, à travers de recherche des fragments de mémoires acquis de diverses sources historiques, on a tenté de faire des investigations sur les transformations urbaines qui ont eu lieu dans la ville au cours des années 50 et 60 du XXe siècle. Ces transformations, dans l espace urbain de Caicó à un moment du pic de l'activité de développement de coton, reflétaient et conditionnaient les propres projets de modernization urbaine qui représentants des élites locales eurent conçu avec l'objectif de la construction d une ville idéal dans les regions isolées du Seridó: moderne, civilisé, progressive et capitale régionale du Seridó. Pour cette construction, les nouveaux équipements et services urbains ont passé du plan imaginaire à l espace réel, installés dans plusieurs quartiers de la ville en transformant le paysage urbain. Face à cette situation, cinémas, magasins, station de radio, énergie électrique, institutions de l'éducation, téléphonie, maison de soins infirmiers, usine de bénéficiement de coton, banques, politiques urbaines, hygiéniques et sanitaires, modes de comportement, sociabilités urbaines distincttes, parmi d'autres éléments géographiques se sont institués à travers la réalisation de certains idéaux de progrès social et dans un esprit de modernité urbaine, dans de nouveaux espaces et pratiques inventés, tramés et éprouvés sur les rochers et entre le cours d eau au milieu d'un quotidien urbain marqué par ruptures et permanence de certaines coutumes et habitudes antiques et de certains paysages et environnement ridé

Morphological and histochemical study of the myotomal muscle in pacu (Piaractus mesopotamicus Holmberg, 1887) during the initial growth phases

Morphological and histochemical methods were used to evaluate the myotomal muscle characteristics of pacu (Piaractus mesopotamicus) from hatching to 40 days old. During the larval period, the musculature consisted predominantly of white muscle. White and red muscle mass increased at 10, 20, 30 and 40 days after hatching. The larvae had round muscle fibers with a moderate degree of maturation and central nuclei. In subsequent phases, small and immature fibers were visible near larger and more differentiated fibers. Undifferentiated cells or presumptive myoblasts located in embryogenic zones were visible in the dorsal and ventral regions, and were more evident at 30 and 40 days. The red muscle fibers located in the subdermal region, had oxidative metabolism and slow contraction, whereas the more predominant white muscle fibers had glycolytic metabolism and fast contraction. Our findings indicate that during the initial phases, myotomal muscle growth in pacu occurs by both, muscle fiber hypertrophy and hyperplasia. The analysis of frequency of red and white muscle fibers shows that hyperplastic growth is intense in this period. As the growth rate in adult fish is related to the number of muscle fibers in young fish, extrinsic factors could change the muscle fiber phenotype and influence their ultimate size.

Ultrastructural features of the glandular region of the stomach of Piaractus mesopotamicus (Holmberg, 1887) with emphasis on the oxyntopeptic cell

The characteristics of the lining and glandular epithelial types of the wall of the stomach of pacu (Piaractus mesopotamicus Holmberg, 1887) were studied by transmission electron microscopy. Columnar mucous cells were observed in the lining epithelial surface and the glandular epithelium that invaginated in the lamina propria showed secretory cells or oxyntopeptic cells as called by some authors in the literature. The columnar epithelial cells are narrow and show a basal nucleus. They are rich in organelles and contain secretory granules of round or rectangular shape heterogeneously electron dense in the apical portion. In the basal region of the glands there are secretory cells. The luminal half of these cells has abundant tubules (tubulo-vesicular system) that communicate with the external medium. Deeper basally in the cytoplasm there are the nucleus, mitochondria of various shapes and other scattered organelles.

Differential expression of myogenic regulatory factor MyoD in pacu skeletal muscle (Piaractus mesopotamicus Holmberg 1887: Serrasalminae, Characidae, Teleostei) during juvenile and adult growth phases

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Stable carbon (delta(13)C) and nitrogen (delta(15)N) isotopes as natural indicators of live and dry food in Piaractus mesopotamicus (Holmberg, 1887) larval tissue

This study proposed the use of the stable isotope technique to track the type of food utilized by pacu Piaractus mesopotamicus larvae during their development, and to identify the moment when the larvae start using nutrients from the dry diet by retaining its carbon and nitrogen atoms in their body tissues. Five-day-old pacu larvae at the onset of exogenous feeding were fed Artemia nauplii or formulated diet exclusively; nauplii+formulated diet during the entire period; or were weaned from nauplii to a dry diet after 3, 6 or 12 days after the first feeding. delta(13)C and delta(15)N values for Artemia nauplii were -15.1 parts per thousand and 4.7 parts per thousand, respectively, and -25.0 parts per thousand and 7.4 parts per thousand for the dry diet. The initial isotopic composition of the larval tissue was -20.2 parts per thousand and 9.5 parts per thousand for delta(13)C and delta(15)N respectively. Later, at the end of a 42-day feeding period, larvae fed Artemia nauplii alone reached values of -12.7 parts per thousand and 7.0 parts per thousand for delta(13)C and delta(15)N respectively. Larvae that received the formulated diet alone showed values of -22.7 parts per thousand for delta(13)C and 9.6 parts per thousand for delta(15)N. The stable isotope technique was precise, and the time at which the larvae utilized Artemia nauplii, and later dry diet as a food source could be clearly defined.

Morphological, histochemical and morphometric study of the myotomal muscle tissue of the pacu (Piaractus mesopotamicus Holmberg 1887: Serrasalminae, Characidae, Teleostei)

Histochemical, ultrastructural and morphometric methods were used to study growth patterns of red, pink and white muscle fibres and their relation to body weight and total length in the fast-growing freshwater fish Piaractus mesopotamicus Holmberg. The correlations amongst body weight, body length and diameter of red, pink and white fibres were low. From 10-15 to 40 50 cm, body weight increased 102.7 times, while the diameter of each type of fibro increased by factors of 0.94, 0.74 and 0.70, respectively. Muscle fibres revealed different morphological and histochemical stages of maturation. The frequencies of < 20 mu-m fibres of red, pink and white muscle tissue in the youngest and oldest classes were 64.5 and 11.0, 38.2 and 7.7 and 24.0 and 1.4%, respectively. In 30-40 cm fish, the frequency of < 20 mu-m fibres in the red and pink tissue was 24.5 and 25.5%, while in the white tissue it was 11.5%. During sexual maturity (40-50 cm), the recruitment of < 20 pm fibres in white muscle was 1.4%. Muscle fibres of this species showed continuous growth by both hyperplastic and hypertrophic mechanisms, and hyperplasia was particularly active in the juvenile phase.

Morphometric study of the ghost crab Ocypode quadrata (Fabricius, 1887) (brachyura, ocypodidae) from Ubatuba, São Paulo, Brazil

A morphometric study of the ghost crab Ocypode quadrata from Ubatuba, São Paulo, Brazil, focusing on the relationships between carapace width and carapace length, abdomen width, gonopod length and propodus length and height of the major cheliped is presented. An allometric positive growth was found for most of the analyzed relationships; however, important changes in the gonopod and abdomen were observed at the size at which sexual maturity is attained. Morphological (external) sexual maturity for both sexes is achieved at a smaller size than that at which gonad maturity is reached. Concerning heterochely, 53.3% of the analyzed specimens exhibited a longer propodus length in the right cheliped.

Morphological, histological, and ultrastructural characterization of degenerating salivary glands in females of the cattle-tick Rhipicephalus (Boophilus) microplus (CANESTRINI, 1887) (Acari : Ixodidae)

The present study describes the morphology and ultrastructure of the salivary glands of semi-engorged females of the southern cattle-tick Rhipicephalus (Boophilus) microplus. The acini that compose these glands, at that specific feeding stage, show cells featuring degenerative process of the salivary glands, such as: vacuolated cytoplasm, condensed chromatin, fragmented nuclei, and presence of apoptotic bodies. In addition, the presence of microorganisms was detected, with morphology typical of protozoa, inside these organs. (c) 2005 Elsevier Ltd. All rights reserved.

Structural and cytochemical changes in the salivary glands of the Rhipicephalus (Boophilus) microplus (CANESTRINI, 1887) (Acari : Ixodidae) tick female during feeding

This study describes the morphology of salivary glands of Rhipicephalus (Boophilus) microplus female ticks at beginning of feeding (24-48 h of attachment) and semi-engorged (4-5 days of attachment) to verify the degenerative characteristics of these organs and the secretory phase in which the process begins. At the beginning of feeding, secretion granules had been observed only in the cytoplasm of cells b, c(1), c(2), c(4) (type II acinus) and d (type III acinus), as well as large nuclei with regular and preserved morphology. In the semi-engorged females the acini presented few normal cells, few partially preserved ones, and the remaining ones in several stages of degeneration, that is, with retraction and cytoplasmic vacuolization, and nuclei with chromatin in several stages of condensation, picnotic and/or in fragmentation. In type I acinus and in the excretory ducts of the studied glands, at both feeding stages, no degenerative characteristic was observed. In females of R. (B.) microplus, the salivary glands degenerate asynchronically and precociously when compared with those of others tick's species. (c) 2006 Elsevier B.V. All rights reserved.