Changes in the structure and pigmentation of the eyes of honeybee (Apis mellifera L.) queens with the limão mutation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

TEMPORAL COUPLING BETWEEN EXTERNAL AUDITORY INFORMATION AND THE PHASES OF WALKING

The purpose of this study was to investigate the effects of an imposed external auditory constraint upon the temporal organization of walking. Ten subjects were videotaped walking normally (N) and with instructions to couple naturally, at mid-swing, or at toe-off to a metronome beat. Based upon an analysis of variance and post hoc Scheffe tests most temporal variables were not significantly different among conditions. The duration of swing phase was significantly different between natural coupling and toe-off. The deviation from the metronome beat was significantly different between the natural coupling and both mid-swing and toe-off. Subjects generally were not successful in achieving coupling during the latter conditions. Thigh and shank phase portraits were used to describe the system's organization to the external constraint.

Urocortin in the central nervous system of a primate (Cebus apella): Sequencing, immunohistochemical, and hybridization histochemical characterization

The urocortin (UCN)-like immunoreactivity and UCN mRNA distribution in various regions of the nonprimate mammalian brain have been reported. However, the Edinger-Westphal nucleus (EW) appears to be the only brain site where UCN expression is conserved across species. Although UCN peptides are present throughout vertebrate phylogeny, the functional roles of both UCN and EW remain poorly understood. Therefore, a study focused on UCN system organization in the primate brain is warranted. By using immunohistochemistry (single and double labeling) and in situ hybridization, we have characterized the organization of UCN-expressing cells and fibers in the central nervous system and pituitary of the capuchin monkey (Cebus apella). In addition, the sequence of the prepro-UCN was determined to establish the level of structural conservation relative to the human sequence. To understand the relationship of acetylcholine cells in the EW, a colocalization study comparing choline acetyltransferase (ChAT) and UCN was also performed. The cloned monkey prepro-UCN is 95% identical to the human preprohormone across the matched sequences. By using an antiserum raised against rat UCN and a probe generated from human cDNA, we found that the EW is the dominant site for UCN expression, although UCN mRNA is also expressed in spinal cord lamina IX. Labeled axons and terminals were distributed diffusely throughout many brain regions and along the length of the spinal cord. of particular interest were UCN-immunoreactive inputs to the medial preoptic area, the paraventricular nucleus of the hypothalamus, the oral part of the spinal trigeminal nucleus, the flocculus of the cerebellum, and the spinal cord laminae VII and X. We found no UCN hybridization signal in the pituitary. In addition, we observed no colocalization between ChAT and UCN in EW neurons. Our results support the hypothesis that the UCN system might participate in the control of autonomic, endocrine, and sensorimotor functions in primates.

Chondroitin sulfate proteoglycans are structural renewable constituents of the rabbit vitreous body

Purpose: To characterize the vitreous intrinsic proteoglycans, investigate their dynamics, and examine their role in the supramolecular organization of the vitreous. Methods: Vitreous from normal rabbits was collected and processed for observation with the transmission electron microscope after treatment with glycosidases. Also, rabbits were injected intravitreally with [S-35]-sodium sulfate and sacrificed at several time intervals after the injection. Proteoglycans (PGs) were assayed in the vitreous supernatant or in whole samples extracted with guanidine hydrochloride by polyacrylamide or agarose gel electrophoresis, followed respectively by fluorography or autoradiography, and ion-exchange chromatography and gel-filtration chromatography, combined with glycolytic treatment of the samples. The sulfated glycosaminoglycans (GAGs) were characterized by agarose gel electrophoresis after treating vitreous samples with protease and specific glycosidases. Results: the electron microscopic study revealed a network with hyaluronic acid ( HA) as thin threads coating and connecting collagen fibrils. The elimination of the HA coat showed chondroitin sulfate granules (8-25 nm) arranged at regular intervals on the fibril surface. The chondroitinase ABC digestion, besides removing the granules, also caused the formation of thicker bundles of the collagen fibrils. The PG and GAG analysis indicated that there are three renewable PGs in the vitreous ( e. g., one heparan-and two chondroitin-sulfate ones). Conclusions: At least one of the chondroitin sulfate PGs is involved in the interactions that occur in the vitreous structure, mainly by providing adequate spacing between the collagen fibrils, a condition that is probably required for the transparency of the vitreous.

Glasses in the SbPO4-WO3 system

Glasses in the binary system (100 - x)SbPO4-xWO3 (20 <= x <= 60, x in mol%) have been prepared and characterized. Differential thermal analysis (DTA) shows that the glass transition temperature, T-g increases from 412 degrees C, for samples containing 20 mol% of WO3 to 481 degrees C observed for glass containing 60 mol%. Sample containing 40 mol% in WO3 were observed to be the most stable against devitrification. The structural organization of the glasses has been studied by using Fourier transform infra-red (FTIR), Raman, P-31 Magic angle spinning (MAS) and spin echo nuclear magnetic resonance (NMR) spectroscopies. Results suggest two distinct networks comprising the glass structure, one with high SbPO4 content and the other characteristic of the highest WO3 content samples. The glasses present photochromic properties. Colour changes are observed for samples after exposure to ultraviolet or visible laser light. XANES, at L-1 absorption edge of tungsten, suggests partial reduction from W6+ to W5+ species during the laser irradiation. The photochromic effects and the colour changes, promoted by laser excitation, are reversible and easily removed by heat for during 1 h at 150 degrees C. Subsequent 'write/erase' cycles can be done without degradation of the glasses. (C) 2007 Elsevier B.V. All rights reserved.

Hyperossification in Miniaturized Toadlets of the genus Brachycephalus (Amphibia: Anura: Brachycephalidae): Microscopic Structure and Macroscopic Patterns of Variation

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influence of the modifier on the short and long range disorder of stannate perovskites

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fine structure of the boundary tissue of the seminiferous tubuli of the vampire bat (Desmodus rotundus, G.; Microchiroptera).

Structurally the boundary tissue of the vampire bat seminiferous tubuli showed 2 to 5 layers of connective tissue in which elongated contractile cells and lamellar and/or fibrillar collagen were noticed. This boundary tissue forms the seminiferous tubular lamina propria. Its structure was more complex around the seminiferous tubuli near the Capsula testicularis than between the adjacent and contiguous tubuli into the testicular lobuli. The whole ultrastructural organization of the seminiferous lamina propria was described here.

Modernizing the Brazilian active control network

The Brazilian Network for Continuous Monitoring of GPS - RBMC, since its foundation in December of 1996, has been playing an essential role for the maintenance and user access of the fundamental geodetic frame in the country,. It provides users with a direct link to the Brazilian Geodetic System - SGB. Its role has become more relevant with the increasing use of space navigation technology in the country. Recently, Brazil adopted a new geodetic system, SIRGAS2000, in February 2005, fully compatible with GNSS technology. The paper provides an overview of the recent modernization phases the RBMC network has undergone highlighting its future steps. From its current post-mission mode, the RBMC will evolve into a real-time network, providing real-time data and real-time correction to users. The network enhanced with modern GPS receivers and the addition of atomic clocks will be used to compute WADGPS-type corrections to be transmitted, in real time, to users in Brazil and surrounding areas. It is estimated that users will be able to achieve a horizontal accuracy around 0.5 m (1σ) in static and kinematic positioning and better for dual frequency users. The availability of the WADGPS service will allow users to tie to the new SIRGAS2000 system in a more rapid and transparent way for positioning and navigation applications. It should be emphasized that support to post-mission static positioning will continue to be provided to users interested in higher accuracy levels. In addition to this, a post-mission Precise Point Positioning (PPP) service will be provided based on the one currently provided by the Geodetic Survey Division of NRCan (CSRS-PPP). The modernization of the RBMC is under development based on a cooperation signed at the end of 2004 with the University of New Brunswick, supported by the Canadian International Development Agency and the Brazilian Cooperation Agency. The Geodetic Survey Division of NRCan is also participating in this modernization effort under the same project.

A comparative study of cytoarchitecture and serotonergic afferents in the suprachiasmatic nucleus of primates (Cebus apella and Callithrix jacchus) and rats (Wistar and Long Evans strains)

The suprachiasmatic nucleus, an essential diencephalic component of the circadian timing system, plays a role in the generation and modulation of behavioral and neuroendocrine rhythms in mammals. Its cytoarchitecture, neurochemical and hodological characteristics have been investigated in various mammalian species, particularly in rodents. In most species, two subdivisions, based on these aspects and considered to reflect functional specialization within the nucleus, can be recognized. Many studies reveal a typical dense innervation by serotonergic fibers in this nucleus, mainly in the ventromedial area, overlapping the retinal afferents. However, a different pattern occurs in certain animals, which lead us to investigate the distribution of serotonergic afferents in the suprachiasmatic nucleus of the Capuchin monkey, Cebus apella, compared to the marmoset, Callithrix jacchus, and two Rattus norvegicus lines (Long Evans and Wistar), and to reported findings for other mammalian species. Our morphometric data show the volume and length of the suprachiasmatic nucleus along the rostrocaudal axis to be greatest in C. apella > C. jacchus > Long Evans ≥ Wistar rats, in agreement with their body sizes. In C. apella, however, the serotonergic terminals occupy only some 10% of the nucleus' area, less than the 25% seen in the marmoset and rats. The distribution of the serotonergic fibers in C. apella does not follow the characteristic ventral organization pattern seen in the rodents. These findings raise questions concerning the intrinsic organization of the nucleus, as well as regarding the functional relationship between serotonergic input and retinal afferents in this diurnal species. © 2007 Elsevier B.V. All rights reserved.

Latest Enhancements in the Brazilian Active Control Network

The Brazilian Network for Continuous Monitoring of GPS - RBMC, since its foundation in December of 1996, has been playing an essential role for the maintenance and user access of the fundamental geodetic frame in the country. It provides to users a direct link to the Brazilian Geodetic System. Its role has become more relevant with the increasing use of space navigation technology in the country. Recently, Brazil adopted a new geodetic frame, SIRGAS2000, in February 2005, fully compatible with GNSS technology. The paper provides an overview of the recent modernization phases the RBMC network has undergone highlighting its future steps. From its current post-mission mode, the RBMC will evolve into a real-time network, providing real-time data and real-time correction to users. The network enhanced with modern GPS receivers and the addition of atomic clocks will be used to compute WADGPS-type corrections to be transmitted, in real time, to users in Brazil and surrounding areas. It is estimated that users will be able to achieve a horizontal accuracy around 0.5 m (1 σ) in static and kinematic positioning and better for dual frequency users. The availability of the WADGPS service will allow users to tie to the new SIRGAS2000 frame in a more rapid and transparent way for positioning and navigation applications. It should be emphasized that support to post-mission static positioning, will continue to be provided to users interested in higher accuracy levels. In addition to this, a post-mission Precise Point Positioning (PPP) service will be provided based on the one currently provided by the Geodetic Survey Division of NRCan (CSRS-PPP). The modernization of the RBMC is under development based on a cooperation signed at the end of 2004 with the University of New Brunswick, supported by the Canadian International Development Agency and the Brazilian Cooperation Agency. The Geodetic Survey Division of NRCan is also participating in this modernization effort under the same project. © Springer-Verlag Berlin Heidelberg 2009.

Growth with stability: financing for development in the new international context

Includes bibliography

Millennium development goals: progress towards the right to health in Latin america and the Caribbean

Includes bibliography

Millennium development goals: advances in environmentally sustainable development in Latin America and the Caribbean

Includes bibliography