Repeat-Associated Fission Yeast-Like Regional Centromeres in the Ascomycetous Budding Yeast Candida tropicalis

The centromere, on which kinetochore proteins assemble, ensures precise chromosome segregation. Centromeres are largely specified by the histone H3 variant CENP-A (also known as Cse4 in yeasts). Structurally, centromere DNA sequences are highly diverse in nature. However, the evolutionary consequence of these structural diversities on de novo CENP-A chromatin formation remains elusive. Here, we report the identification of centromeres, as the binding sites of four evolutionarily conserved kinetochore proteins, in the human pathogenic budding yeast Candida tropicalis. Each of the seven centromeres comprises a 2 to 5 kb non-repetitive mid core flanked by 2 to 5 kb inverted repeats. The repeat-associated centromeres of C. tropicalis all share a high degree of sequence conservation with each other and are strikingly diverged from the unique and mostly non-repetitive centromeres of related Candida species-Candida albicans, Candida dubliniensis, and Candida lusitaniae. Using a plasmid-based assay, we further demonstrate that pericentric inverted repeats and the underlying DNA sequence provide a structural determinant in CENP-A recruitment in C. tropicalis, as opposed to epigenetically regulated CENP-A loading at centromeres in C. albicans. Thus, the centromere structure and its influence on de novo CENP-A recruitment has been significantly rewired in closely related Candida species. Strikingly, the centromere structural properties along with role of pericentric repeats in de novo CENP-A loading in C. tropicalis are more reminiscent to those of the distantly related fission yeast Schizosaccharomyces pombe. Taken together, we demonstrate, for the first time, fission yeast-like repeat-associated centromeres in an ascomycetous budding yeast.

High-Resolution Finite Compact Difference Schemes for Hyperbolic Conservation Laws

A finite compact (FC) difference scheme requiring only bi-diagonal matrix inversion is proposed by using the known high-resolution flux. Introducing TVD or ENO limiters in the numerical flux, several high-resolution FC-schemes of hyperbolic conservation law are developed, including the FC-TVD, third-order FC-ENO and fifth-order FC-ENO schemes. Boundary conditions formulated need only one unknown variable for third-order FC-ENO scheme and two unknown variables for fifth-order FC-ENO scheme. Numerical test results of the proposed FC-scheme were compared with traditional TVD, ENO and WENO schemes to demonstrate its high-order accuracy and high-resolution.

Signature-tagged transposon mutagenesis studies demonstrate the dynamic nature of cecal colonization of 2-week-old chickens by Campylobacter jejuni.

We have constructed plasmids to be used for in vitro signature-tagged mutagenesis (STM) of Campylobacter jejuni and used these to generate STM libraries in three different strains. Statistical analysis of the transposon insertion sites in the C. jejuni NCTC 11168 chromosome and the plasmids of strain 81-176 indicated that their distribution was not uniform. Visual inspection of the distribution suggested that deviation from uniformity was not due to preferential integration of the transposon into a limited number of hot spots but rather that there was a bias towards insertions around the origin. We screened pools of mutants from the STM libraries for their ability to colonize the ceca of 2-week-old chickens harboring a standardized gut flora. We observed high-frequency random loss of colonization proficient mutants. When cohoused birds were individually inoculated with different tagged mutants, random loss of colonization-proficient mutants was similarly observed, as was extensive bird-to-bird transmission of mutants. This indicates that the nature of campylobacter colonization in chickens is complex and dynamic, and we hypothesize that bottlenecks in the colonization process and between-bird transmission account for these observations.

Nature of disorder and localization in amorphous carbon

The electronic structure of amorphous diamond-like carbon is studied. Analysis of the participation ratio shows that π states within the σ-σ* gap are localized. The localization arises from dihedral angle disorder. The localization of π states causes the mobility gap to exceed the optical gap, which accounts for the low carrier mobility and the flat photoluminesence excitation spectrum. © 1998 Elsevier Science B.V. All rights reserved.

Redescubrir la finalidad natural : Robert Spaemann y Tomás de Aquino

Resumen: En ocasión de la autobiografía de Robert Spaemann aparecida en 2012, este trabajo examina la lectura que Spaemann hace de la teleología en la doctrina de Santo Tomás de Aquino, especialmente en su obra Natürliche Ziele (2005) que prosigue la versión de 1981. En la primera parte, se estudia la historia de la teleología en el pensamiento filosófico y científico expuesta en «Fines naturales». La finalidad intrínseca en la naturaleza de los seres es conocida con profundidad creciente gracias a Platón, Aristóteles, al estoicismo y a la tradición cristiana. Estos aportes se integran en una estructura teleológica unitaria en el Aquinate. Después acontece una «inversión» de la teleología centrada en la conservación del propio ser y una consideración de los fines como algo externo a la realidad. Con la teoría evolucionista surge la teleonomía que se limita a las reglas del dinamismo, suprimiendo el fin. Con todo, los científicos no logran eliminar los términos «con el fin de» o «para» en su lenguaje. Eso permite proceder a un redescubrimiento de los fines naturales. En la segunda parte, se ve cómo Tomás de Aquino lleva el conocimiento de la finalidad más allá de Aristóteles, porque ella remite a una inteligencia creadora. La teleología implica una teología, aunque no queda del todo claro si es la teología filosófica o la cristiana. Spaemann tiene un planteo ontológico de tipo persuasivo, pero en algunos momentos siente dificultad para superar en el plano teorético a quien se limita a una teleonomía.

El objeto formal de la vis cogitativa en Santo Tomás de Aquino

Resumen: De acuerdo a la mente de Santo Tomás de Aquino, la potencia de conocimiento sensible que se denomina «cogitativa » es susceptible de una doble consideración. Por un lado, es la estimativa animal y, como tal, aprehende su mismo objeto formal quod, esto es, las intenciones de beneficio o perjuicio a nivel sensible. Pero a causa de su particular modo de operar, esas intenciones no son conocidas por ella solamente en orden a la conservación del individuo humano o de la especie a la que él pertenece. El reconocimiento de una doble dimensión de la cogitativa está en Santo Tomás justificado por la particular relación que existe entre ella y la razón universal. Por un lado, la cogitativa no es una facultad distinta de la estimativa natural. Por esto, ella aprehende las intenciones necesarias para la perfección de la vida sensitiva humana. Por otro lado, la razón ejerce sobre la cogitativa un influjo particular que la hace operar a su modo, esto es, de manera discursiva. Este carácter móvil en la aprehensión de la cogitativa permite la conformación de una experiencia a nivel sensible, en virtud de la cual su objeto formal se ve ampliado. Esto resulta aún más razonable si se tiene en cuenta que el conocimiento sensorial del hombre prepara el intelectual. De esta manera, la cogitativa no aprehende simplemente al individuo como beneficioso o perjudicial para la subsistencia del cognoscente, sino como sujeto de una cierta naturaleza.

An Overview on the Field of Micro- and Nanotechnologies for Synthetic Peptide-Based Vaccines

18 p.

Charting multidisciplinary research and action priorities towards the conservation and sustainable management of sea turtles in the Pacific Ocean: a focus on Malaysia

The four sea turtle species found in Malaysia are the leatherback, olive ridley, green and hawksbill. The threats to these species are acute. Populations of leatherback, olive ridley and hawksbill turtles are on the brink of collapse – threatening a biodiversity crisis in Malaysia and the region. This proceedings contains 8 technical papers presented at a workshop convened in Kijal, Terengganu to chart new directions in the conservation of Malaysia's critically endangered sea turtles and to reverse population decline. They represent a wide range of issues from aspects of biology to a review of 40 years of sea turtle conservation. A paper on the socioeconomic linkages and impacts of fisheries was also included as the workshop adopted a multidisciplinary approach to address the issues. Two case studies, including successful restoration examples from international experiences and restoration efforts in Sabah, pave the way for enhancing turtle conservation in the country.

Strategic planning for long-term financing of Pacific leatherback conservation and recovery:Proceedings of the Bellagio Sea Turtle Conservation Initiative, Terengganu, Malaysia; July 2007

On 17-20 July 2007, 45 experts on sea turtles, fisheries, conservation and finance from 10 countries convened at the Bellagio Sea Turtle Conservation Initiative workshop in Terengganu to focus on methods to save the imperiled Pacific leatherback from extinction. The group developed a strategic plan to guide the prioritization and long term financing of Pacific leatherback turtle conservation and recovery objectives. Participants identified critical conservation actions and agreed that a business plan is urgently needed to reverse the trajectory towards extinction of the Pacific leatherback. The conservation actions prioritized by the participants encompassed protecting nesting beaches including eggs and nesting females; reducing direct and indirect turtle take in coastal fisheries; and strengthening regional and sub-regional cooperation. The group committed to work together on fundraising and implementation of these urgent conservation actions. This report presents outputs and the plan that was produced from the workshop.

Catch-22: conservation, communities and the privatization of B.C. fisheries. An economic, social and ecological impact study

pdf contains 47 pages

Santa Fe River watershed conservation lands

Conservation lands, which are essential to protecting water resources in the Santa Fe River basin.

Information Preservation (IP) Method in Simulation

This paper reviews firstly methods for treating low speed rarefied gas flows: the linearised Boltzmann equation, the Lattice Boltzmann method (LBM), the Navier-Stokes equation plus slip boundary conditions and the DSMC method, and discusses the difficulties in simulating low speed transitional MEMS flows, especially the internal flows. In particular, the present version of the LBM is shown unfeasible for simulation of MEMS flow in transitional regime. The information preservation (IP) method overcomes the difficulty of the statistical simulation caused by the small information to noise ratio for low speed flows by preserving the average information of the enormous number of molecules a simulated molecule represents. A kind of validation of the method is given in this paper. The specificities of the internal flows in MEMS, i.e. the low speed and the large length to width ratio, result in the problem of elliptic nature of the necessity to regulate the inlet and outlet boundary conditions that influence each other. Through the example of the IP calculation of the microchannel (thousands long) flow it is shown that the adoption of the conservative scheme of the mass conservation equation and the super relaxation method resolves this problem successfully. With employment of the same measures the IP method solves the thin film air bearing problem in transitional regime for authentic hard disc write/read head length ( ) and provides pressure distribution in full agreement with the generalized Reynolds equation, while before this the DSMC check of the validity of the Reynolds equation was done only for short ( ) drive head. The author suggests degenerate the Reynolds equation to solve the microchannel flow problem in transitional regime, thus provides a means with merit of strict kinetic theory for testing various methods intending to treat the internal MEMS flows.