Integration of Timetable Planning and Rolling Stock in Rapid Transit Networks

The aim of this paper is to propose an integrated planning model to adequate the offered capacity and system frequencies to attend the increased passenger demand and traffic congestion around urban and suburban areas. The railway capacity is studied in line planning, however, these planned frequencies were obtained without accounting for rolling stock flows through the rapid transit network. In order to provide the problem more freedom to decide rolling stock flows and therefore better adjusting these flows to passenger demand, a new integrated model is proposed, where frequencies are readjusted. Then, the railway timetable and rolling stock assignment are also calculated, where shunting operations are taken into account. These operations may sometimes malfunction, causing localized incidents that could propagate throughout the entire network due to cascading effects. This type of operations will be penalized with the goal of selectively avoiding them and ameliorating their high malfunction probabilities. Swapping operations will also be ensured using homogeneous rolling stock material and ensuring parkings in strategic stations. We illustrate our model using computational experiments drawn from RENFE (the main Spanish operator of suburban passenger trains) in Madrid, Spain. The results show that through this integrated approach a greater robustness degree can be obtained

Low-speed longitudinal controllers for mass-produced cars: A comparative study

Four longitudinal control techniques are compared: a classical Proportional-Integral (PI) control; an advanced technique-called the i-PI-that adds an intelligent component to the PI; a fuzzy controller based on human experience; and an adaptive-network-based fuzzy inference system. The controllers were designed to tackle one of the challenging topics as yet unsolved by the automotive sector: managing autonomously a gasoline-propelled vehicle at very low speeds. The dynamics involved are highly nonlinear and constitute an excellent test-bed for newly designed controllers. A Citroën C3 Pluriel car was modified to permit autonomous action on the accelerator and the brake pedals-i.e., longitudinal control. The controllers were tested in two stages. First, the vehicle was modeled to check the controllers' feasibility. Second, the controllers were then implemented in the Citroën, and their behavior under the same conditions on an identical real circuit was compared.

Effect of impacting radius of curvature on firmness sensing of fruits in a sorting system.

Fruits of two varieties of both apples and pear were tested to measure their response to small energy impact applied by a impact tester with two spherical tips of different radious of curvature ( RA = 2.48 cm and RB = 0.98 cm) and equal mass were used. In the four varieties studied, the size of bruise was smaller with a spherical tip RA than with tip RB . The non-destructive impact test would cause less damage with a spherical impactor with a radious bigger than 0.98 cm.

Performance of coupled ED-tether/ion thrsuter system

Use of propulsion systems that couple electyrodynamic tethers to ion thrusters, as suggested in the literature, is discussed. The system establishes electrical contact with the ionospheric plasma, at the anodic end of the tether, by ejecting ions instead of collecting electrons; also, the ion thruster adds its thrust to the Lorentz force on the tether. In this paper, we analyze the performance of this coupled system, as measured by the ratio of mission impulse (thrust times mission duration) to the overall system mass, which includes the power subsystem mass, the tether subsystem mass, and the propellant mass consumed in the ion thruster. It is shown that a tether acting by itself, collecting electrons at its anodic end, substantially outperforms the coupled system for times longer than a characteristic time of the ion thruster, for which propellant mass equals the power subsystem mass; for shorter times performances are shown to be similar.

System Engineering Approach to Initial Design of LEO Remote Sensing Missions

The satellite remote sensing missions are essential for long-term research around the condition of the earth resources and environment. On the other hand, in recent years the application of microsatellites is of interest in many space programs for their less cost and response time. In microsatellite remote sensing missions there are tight interrelations between different requirements such as orbital altitude, revisit time, mission life and spatial resolution. Also, all of these requirements can affect the whole system level design characteristics. In this work, the remote sensing microsatellite sizing process is divided into three major design disciplines; a) orbit design, b) payload sizing and c) bus sizing. Finally, some specific design cases are investigated inside the design space for evaluating the effect of different design variables on the satellite total mass. Considering the results of the work, it is concluded that applying a systematic approach at the initial design phase of such projects provides a good insight to the not clearly seen interactions inside their highly extended design space

An universal system to de-orbit satellites at end of life

A 3-year Project financed by the European Commission is aimed at developing a universal system to de-orbit satellites at their end of life, as a fundamental contribution to limit the increase of debris in the Space environment. The operational system involves a conductive tapetether left bare to establish anodic contact with the ambient plasma as a giant Langmuir probe. The Project will size the three disparate dimensions of a tape for a selected de-orbit mission and determine scaling laws to allow system design for a general mission. Starting at the second year, mission selection is carried out while developing numerical codes to implement control laws on tether dynamics in/off the orbital plane; performing numerical simulations and plasma chamber measurements on tether-plasma interaction; and completing design of subsystems: electronejecting plasma contactor, power module, interface elements, deployment mechanism, and tether-tape/end-mass. This will be followed by subsystems manufacturing and by currentcollection, free-fall, and hypervelocity impact tests.

Distribution of the coal flow in the mill-duct system of the As Pontes Power Plant using CFD modeling

The efficiency of a Power Plant is affected by the distribution of the pulverized coal within the furnace. The coal, which is pulverized in the mills, is transported and distributed by the primary gas through the mill-ducts to the interior of the furnace. This is done with a double function: dry and enter the coal by different levels for optimizing the combustion in the sense that a complete combustion occurs with homogeneous heat fluxes to the walls. The mill-duct systems of a real Power Plant are very complex and they are not yet well understood. In particular, experimental data concerning the mass flows of coal to the different levels are very difficult to measure. CFD modeling can help to determine them. An Eulerian/Lagrangian approach is used due to the low solid–gas volume ratio.

Diseño acústico y electroacústico de Waipahu Transit Center en Honolulú, Hawái

El objetivo de este Proyecto Fin de Grado es el diseño de megafonía y PAGA (Public Address /General Alarm) de la estación de tren Waipahu Transit Center en la ciudad de Honolulú, Hawái. Esta estación forma parte de una nueva línea de tren que está en proceso de construcción actualmente llamada Honolulu Rail Transit. Inicialmente la línea de tren constará de 21 estaciones, en las que prácticamente todas están diseñadas como pasos elevados usando como referencia las autopistas que cruzan la isla. Se tiene prevista su fecha de finalización en el año 2019, aunque las primeras estaciones se inaugurarán en 2017. Se trata en primer lugar un estudio acústico del recinto a sonorizar, eligiendo los equipos necesarios: conmutadores, altavoces, amplificadores, procesador, equipo de control y micrófonos. Este primer estudio sirve para obtener una aproximación de equipos necesarios, así como la posible situación de estos dentro de la estación. Tras esto, se procede a la simulación de la estación mediante el programa de simulación acústica y electroacústica EASE 4.4. Para ello, se diseña la estación en un modelo 3D, en el que cada superficie se asocia a su material correspondiente. Para facilitar el diseño y el cómputo de las simulaciones se divide la estación en 3 partes por separado. Cada una corresponde a un nivel de la estación: Ground level, el nivel inferior que contiene la entrada; Concourse Level, pasillo que comunica los dos andenes; y Platform Level, en el que realizarán las paradas los trenes. Una vez realizado el diseño se procede al posicionamiento de altavoces en los diferentes niveles de la estación. Debido al clima existente en la isla, el cual ronda los 20°C a lo largo de todo el año, no es necesaria la instalación de sistemas de aire acondicionado o calefacción, por lo que la estación no está totalmente cerrada. Esto supone un problema al realizar las simulaciones en EASE, ya que al tratarse de un recinto abierto se deberán hallar parámetros como el tiempo de reverberación o el volumen equivalente por otros medios. Para ello, se utilizará el método Ray Tracing, mediante el cual se halla el tiempo de reverberación por la respuesta al impulso de la sala; y a continuación se calcula un volumen equivalente del recinto mediante la fórmula de Eyring. Con estos datos, se puede proceder a calcular los parámetros necesarios: nivel de presión sonora directo, nivel de presión sonora total y STI (Speech Transmission Index). Para obtener este último será necesario ecualizar antes en cada uno de los niveles de la estación. Una vez hechas las simulaciones, se comprueba que el nivel de presión sonora y los valores de inteligibilidad son acordes con los requisitos dados por el cliente. Tras esto, se procede a realizar los bucles de altavoces y el cálculo de amplificadores necesarios. Se estudia la situación de los micrófonos, que servirán para poder variar la potencia emitida por los altavoces dependiendo del nivel de ruido en la estación. Una vez obtenidos todos los equipos necesarios en la estación, se hace el conexionado entre éstos, tanto de una forma simplificada en la que se pueden ver los bucles de altavoces en cada nivel de la estación, como de una forma más detallada en la que se muestran las conexiones entre cada equipo del rack. Finalmente, se realiza el etiquetado de los equipos y un presupuesto estimado con los costes del diseño del sistema PAGA. ABSTRACT. The aim of this Final Degree Project is the design of the PAGA (Public Address / General Alarm) system in the train station Waipahu Transit Center in the city of Honolulu, Hawaii. This station is part of a new rail line that is currently under construction, called Honolulu Rail Transit. Initially, the rail line will have 21 stations, in which almost all are designed elevated using the highways that cross the island as reference. At first, it is treated an acoustic study in the areas to cover, choosing the equipment needed: switches, loudspeakers, amplifiers, DPS, control station and microphones. This first study helps to obtain an approximation of the equipments needed, as well as their placement inside the station. Thereafter, it is proceeded to do the simulation of the station through the acoustics and electroacoustics simulation software EASE 4.4. In order to do that, it is made the 3D design of the station, in which each surface is associated with its material. In order to ease the design and calculation of the simulations, the station has been divided in 3 zones. Each one corresponds with one level of the station: Ground Level, the lower level that has the entrance; Concourse Level, a corridor that links the two platforms; and Platform Level, where the trains will stop. Once the design is made, it is proceeded to place the speakers in the different levels of the station. Due to the weather in the island, which is about 20°C throughout the year, it is not necessary the installation of air conditioning or heating systems, so the station is not totally closed. This cause a problem when making the simulations in EASE, as the project is open, and it will be necessary to calculate parameters like the reverberation time or the equivalent volume by other methods. In order to do that, it will be used the Ray Tracing method, by which the reverberation time is calculated by the impulse response; and then it is calculated the equivalent volume of the area with the Eyring equation. With this information, it can be proceeded to calculate the parameters needed: direct sound pressure level, total sound pressure level and STI (Speech Transmission Index). In order to obtain the STI, it will be needed to equalize before in each of the station’s levels. Once the simulations are done, it is checked that the sound pressure level and the intelligibility values agree with the requirements given by the client. After that, it is proceeded to perform the speaker’s loops and the calculation of the amplifiers needed. It is studied the placement of the microphones, which will help to vary the power emitted by the speakers depending on the background noise level in the station. Once obtained all the necessary equipment in the station, it is done the connection diagram, both a simplified diagram in which there can be seen the speaker’s loops in each level of the station, or a more detailed diagram in which it is shown the wiring between each equipment of the rack. At last, it is done the labeling of the equipments and an estimated budget with the expenses for the PAGA design.

On a competitive system under chemotactic effects with non-local terms

In this paper, we study a system of partial differential equations describing the evolution of a population under chemotactic effects with non-local reaction terms. We consider an external application of chemoattractant in the system and study the cases of one and two populations in competition. By introducing global competitive/cooperative factors in terms of the total mass of the populations, weobtain, forarangeofparameters, thatanysolutionwithpositive and bounded initial data converges to a spatially homogeneous state with positive components. The proofs rely on the maximum principle for spatially homogeneous sub- and super-solutions.

Sistema de simulación de escenarios de ciclos avanzados de combustible nuclear en transición = Simulation system for advanced fuel cycle transition scenarios

El estudio de los ciclos del combustible nuclear requieren de herramientas computacionales o "códigos" versátiles para dar respuestas al problema multicriterio de evaluar los actuales ciclos o las capacidades de las diferentes estrategias y escenarios con potencial de desarrollo en a nivel nacional, regional o mundial. Por otra parte, la introducción de nuevas tecnologías para reactores y procesos industriales hace que los códigos existentes requieran nuevas capacidades para evaluar la transición del estado actual del ciclo del combustible hacia otros más avanzados y sostenibles. Brevemente, esta tesis se centra en dar respuesta a las principales preguntas, en términos económicos y de recursos, al análisis de escenarios de ciclos de combustible, en particular, para el análisis de los diferentes escenarios del ciclo del combustible de relativa importancia para España y Europa. Para alcanzar este objetivo ha sido necesaria la actualización y el desarrollo de nuevas capacidades del código TR_EVOL (Transition Evolution code). Este trabajo ha sido desarrollado en el Programa de Innovación Nuclear del CIEMAT desde el año 2010. Esta tesis se divide en 6 capítulos. El primer capítulo ofrece una visión general del ciclo de combustible nuclear, sus principales etapas y los diferentes tipos utilizados en la actualidad o en desarrollo para el futuro. Además, se describen las fuentes de material nuclear que podrían ser utilizadas como combustible (uranio y otros). También se puntualizan brevemente una serie de herramientas desarrolladas para el estudio de estos ciclos de combustible nuclear. El capítulo 2 está dirigido a dar una idea básica acerca de los costes involucrados en la generación de electricidad mediante energía nuclear. Aquí se presentan una clasificación de estos costos y sus estimaciones, obtenidas en la bibliografía, y que han sido evaluadas y utilizadas en esta tesis. Se ha incluido también una breve descripción del principal indicador económico utilizado en esta tesis, el “coste nivelado de la electricidad”. El capítulo 3 se centra en la descripción del código de simulación desarrollado para el estudio del ciclo del combustible nuclear, TR_EVOL, que ha sido diseñado para evaluar diferentes opciones de ciclos de combustibles. En particular, pueden ser evaluados las diversos reactores con, posiblemente, diferentes tipos de combustibles y sus instalaciones del ciclo asociadas. El módulo de evaluaciones económica de TR_EVOL ofrece el coste nivelado de la electricidad haciendo uso de las cuatro fuentes principales de información económica y de la salida del balance de masas obtenido de la simulación del ciclo en TR_EVOL. Por otra parte, la estimación de las incertidumbres en los costes también puede ser efectuada por el código. Se ha efectuado un proceso de comprobación cruzada de las funcionalidades del código y se descrine en el Capítulo 4. El proceso se ha aplicado en cuatro etapas de acuerdo con las características más importantes de TR_EVOL, balance de masas, composición isotópica y análisis económico. Así, la primera etapa ha consistido en el balance masas del ciclo de combustible nuclear actual de España. La segunda etapa se ha centrado en la comprobación de la composición isotópica del flujo de masas mediante el la simulación del ciclo del combustible definido en el proyecto CP-ESFR UE. Las dos últimas etapas han tenido como objetivo validar el módulo económico. De este modo, en la tercera etapa han sido evaluados los tres principales costes (financieros, operación y mantenimiento y de combustible) y comparados con los obtenidos por el proyecto ARCAS, omitiendo los costes del fin del ciclo o Back-end, los que han sido evaluado solo en la cuarta etapa, haciendo uso de costes unitarios y parámetros obtenidos a partir de la bibliografía. En el capítulo 5 se analizan dos grupos de opciones del ciclo del combustible nuclear de relevante importancia, en términos económicos y de recursos, para España y Europa. Para el caso español, se han simulado dos grupos de escenarios del ciclo del combustible, incluyendo estrategias de reproceso y extensión de vida de los reactores. Este análisis se ha centrado en explorar las ventajas y desventajas de reprocesado de combustible irradiado en un país con una “relativa” pequeña cantidad de reactores nucleares. Para el grupo de Europa se han tratado cuatro escenarios, incluyendo opciones de transmutación. Los escenarios incluyen los reactores actuales utilizando la tecnología reactor de agua ligera y ciclo abierto, un reemplazo total de los reactores actuales con reactores rápidos que queman combustible U-Pu MOX y dos escenarios del ciclo del combustible con transmutación de actínidos minoritarios en una parte de los reactores rápidos o en sistemas impulsados por aceleradores dedicados a transmutación. Finalmente, el capítulo 6 da las principales conclusiones obtenidas de esta tesis y los trabajos futuros previstos en el campo del análisis de ciclos de combustible nuclear. ABSTRACT The study of the nuclear fuel cycle requires versatile computational tools or “codes” to provide answers to the multicriteria problem of assessing current nuclear fuel cycles or the capabilities of different strategies and scenarios with potential development in a country, region or at world level. Moreover, the introduction of new technologies for reactors and industrial processes makes the existing codes to require new capabilities to assess the transition from current status of the fuel cycle to the more advanced and sustainable ones. Briefly, this thesis is focused in providing answers to the main questions about resources and economics in fuel cycle scenario analyses, in particular for the analysis of different fuel cycle scenarios with relative importance for Spain and Europe. The upgrade and development of new capabilities of the TR_EVOL code (Transition Evolution code) has been necessary to achieve this goal. This work has been developed in the Nuclear Innovation Program at CIEMAT since year 2010. This thesis is divided in 6 chapters. The first one gives an overview of the nuclear fuel cycle, its main stages and types currently used or in development for the future. Besides the sources of nuclear material that could be used as fuel (uranium and others) are also viewed here. A number of tools developed for the study of these nuclear fuel cycles are also briefly described in this chapter. Chapter 2 is aimed to give a basic idea about the cost involved in the electricity generation by means of the nuclear energy. The main classification of these costs and their estimations given by bibliography, which have been evaluated in this thesis, are presented. A brief description of the Levelized Cost of Electricity, the principal economic indicator used in this thesis, has been also included. Chapter 3 is focused on the description of the simulation tool TR_EVOL developed for the study of the nuclear fuel cycle. TR_EVOL has been designed to evaluate different options for the fuel cycle scenario. In particular, diverse nuclear power plants, having possibly different types of fuels and the associated fuel cycle facilities can be assessed. The TR_EVOL module for economic assessments provides the Levelized Cost of Electricity making use of the TR_EVOL mass balance output and four main sources of economic information. Furthermore, uncertainties assessment can be also carried out by the code. A cross checking process of the performance of the code has been accomplished and it is shown in Chapter 4. The process has been applied in four stages according to the most important features of TR_EVOL. Thus, the first stage has involved the mass balance of the current Spanish nuclear fuel cycle. The second stage has been focused in the isotopic composition of the mass flow using the fuel cycle defined in the EU project CP-ESFR. The last two stages have been aimed to validate the economic module. In the third stage, the main three generation costs (financial cost, O&M and fuel cost) have been assessed and compared to those obtained by ARCAS project, omitting the back-end costs. This last cost has been evaluated alone in the fourth stage, making use of some unit cost and parameters obtained from the bibliography. In Chapter 5 two groups of nuclear fuel cycle options with relevant importance for Spain and Europe are analyzed in economic and resources terms. For the Spanish case, two groups of fuel cycle scenarios have been simulated including reprocessing strategies and life extension of the current reactor fleet. This analysis has been focused on exploring the advantages and disadvantages of spent fuel reprocessing in a country with relatively small amount of nuclear power plants. For the European group, four fuel cycle scenarios involving transmutation options have been addressed. Scenarios include the current fleet using Light Water Reactor technology and open fuel cycle, a full replacement of the initial fleet with Fast Reactors burning U-Pu MOX fuel and two fuel cycle scenarios with Minor Actinide transmutation in a fraction of the FR fleet or in dedicated Accelerator Driven Systems. Finally, Chapter 6 gives the main conclusions obtained from this thesis and the future work foreseen in the field of nuclear fuel cycle analysis.

OSIRIS – The scientific camera system onboard Rosetta

The Optical, Spectroscopic, and Infrared Remote Imaging System OSIRIS is the scientific camera system onboard the Rosetta spacecraft (Figure 1). The advanced high performance imaging system will be pivotal for the success of the Rosetta mission. OSIRIS will detect 67P/Churyumov-Gerasimenko from a distance of more than 106 km, characterise the comet shape and volume, its rotational state and find a suitable landing spot for Philae, the Rosetta lander. OSIRIS will observe the nucleus, its activity and surroundings down to a scale of ~2 cm px−1. The observations will begin well before the onset of cometary activity and will extend over months until the comet reaches perihelion. During the rendezvous episode of the Rosetta mission, OSIRIS will provide key information about the nature of cometary nuclei and reveal the physics of cometary activity that leads to the gas and dust coma. OSIRIS comprises a high resolution Narrow Angle Camera (NAC) unit and a Wide Angle Camera (WAC) unit accompanied by three electronics boxes. The NAC is designed to obtain high resolution images of the surface of comet 7P/Churyumov-Gerasimenko through 12 discrete filters over the wavelength range 250–1000 nm at an angular resolution of 18.6 μrad px−1. The WAC is optimised to provide images of the near-nucleus environment in 14 discrete filters at an angular resolution of 101 μrad px−1. The two units use identical shutter, filter wheel, front door, and detector systems. They are operated by a common Data Processing Unit. The OSIRIS instrument has a total mass of 35 kg and is provided by institutes from six European countries

Methodology for instantaneous average exhaust gas mass flow rate measurement

This paper presents a new methodology for measurement of the instantaneous average exhaust mass flow rate in reciprocating internal combustion engines to be used to determinate real driving emissions on light duty vehicles, as part of a Portable Emission Measurement System (PEMS). Firstly a flow meter, named MIVECO flow meter, was designed based on a Pitot tube adapted to exhaust gases which are characterized by moisture and particle content, rapid changes in flow rate and chemical composition, pulsating and reverse flow at very low engine speed. Then, an off-line methodology was developed to calculate the instantaneous average flow, considering the ?square root error? phenomenon. The paper includes the theoretical fundamentals, the developed flow meter specifications, the calibration tests, the description of the proposed off-line methodology and the results of the validation test carried out in a chassis dynamometer, where the validity of the mass flow meter and the methodology developed are demonstrated.

Kinematic geometry of mass-triangles and reduction of Schrödinger’s equation of three-body systems to partial differential equations solely defined on triangular parameters

Schrödinger’s equation of a three-body system is a linear partial differential equation (PDE) defined on the 9-dimensional configuration space, ℝ9, naturally equipped with Jacobi’s kinematic metric and with translational and rotational symmetries. The natural invariance of Schrödinger’s equation with respect to the translational symmetry enables us to reduce the configuration space to that of a 6-dimensional one, while that of the rotational symmetry provides the quantum mechanical version of angular momentum conservation. However, the problem of maximizing the use of rotational invariance so as to enable us to reduce Schrödinger’s equation to corresponding PDEs solely defined on triangular parameters—i.e., at the level of ℝ6/SO(3)—has never been adequately treated. This article describes the results on the orbital geometry and the harmonic analysis of (SO(3),ℝ6) which enable us to obtain such a reduction of Schrödinger’s equation of three-body systems to PDEs solely defined on triangular parameters.

Identification by mass spectrometry of the phosphorylated residue responsible for activation of the catalytic domain of myosin I heavy chain kinase, a member of the PAK/STE20 family

Myosin I heavy chain kinase from Acanthamoeba castellanii is activated in vitro by autophosphorylation (8–10 mol of P per mol). The catalytically active C-terminal domain produced by trypsin cleavage of the phosphorylated kinase contains 2–3 mol of P per mol. However, the catalytic domain expressed in a baculovirus–insect cell system is fully active as isolated without autophosphorylation in vitro. We now show that the expressed catalytic domain is inactivated by incubation with acid phosphatase and regains activity upon autophosphorylation. The state of phosphorylation of all of the hydroxyamino acids in the catalytic domain were determined by mass spectrometry of unfractionated protease digests. Ser-627 was phosphorylated in the active, expressed catalytic domain, lost its phosphate when the protein was incubated with phosphatase, and was rephosphorylated when the dephosphorylated protein was incubated with ATP. No other residue was significantly phosphorylated in any of the three samples. Thus, phosphorylation of Ser-627, which is in the same position as the Ser and Thr residues that are phosphorylated in many other kinases, is necessary and sufficient for full activity of the catalytic domain. Ser-627 is also phosphorylated when full-length, native kinase is activated by autophosphorylation.

The chemistry of the S-nitrosoglutathione/glutathione system

S-Nitrosothiols have generated considerable interest due to their ability to act as nitric oxide (NO) donors and due to their possible involvement in bioregulatory systems—e.g., NO transfer reactions. Elucidation of the reaction pathways involved in the modification of the thiol group by S-nitrosothiols is important for understanding the role of S-nitroso compounds in vivo. The modification of glutathione (GSH) in the presence of S-nitrosoglutathione (GSNO) was examined as a model reaction. Incubation of GSNO (1 mM) with GSH at various concentrations (1–10 mM) in phosphate buffer (pH 7.4) yielded oxidized glutathione, nitrite, nitrous oxide, and ammonia as end products. The product yields were dependent on the concentrations of GSH and oxygen. Transient signals corresponding to GSH conjugates, which increased by one mass unit when the reaction was carried out with 15N-labeled GSNO, were identified by electrospray ionization mass spectrometry. When morpholine was present in the reaction system, N-nitrosomorpholine was formed. Increasing concentrations of either phosphate or GSH led to lower yields of N-nitrosomorpholine. The inhibitory effect of phosphate may be due to reaction with the nitrosating agent, nitrous anhydride (N2O3), formed by oxidation of NO. This supports the release of NO during the reaction of GSNO with GSH. The products noted above account quantitatively for virtually all of the GSNO nitrogen consumed during the reaction, and it is now possible to construct a complete set of pathways for the complex transformations arising from GSNO + GSH.