956 resultados para Isabella I, Queen of Spain, 1451-1504.


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This paper applies an integrated modeling approach to the case of Spain; the approach is based on a random utility-based multiregional input-output model and a road transport network model for assessing the effect of introducing longer and heavier vehicles (LHVs) on the regional consumer price index (CPI) and on the transportation system. The approach strongly supports the concept that changes in transport costs derived from the LHV allowance as well as the economic structure of regions have direct and indirect effects on the economy and on the transportation system. Results show that the introduction of LHVs might reduce prices paid by consumers for a representative basket of goods and services in the regions of Spain and would also lead to a reduction in the regional CPI. In addition, the magnitude and extent of changes in the transportation system are estimated by using the commodity-based structure of the approach to identify the effect of traffic changes on traffic flows and on pollutant emissions over the whole network.

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In the centre of Castilla y León (extensive zone of Spain) still there are some examples of the old rural buildings linked to the economic activities that have been developed in the agrarian field. These are called chozos and casetas, and they have been erected with domed solutions using the autochthonous materi-als: the mud in the region of Tierra de Campos and the stone in the region of Montes Torozos. The influence of traditional construction techniques of both elements has generated a singular typological range rarely seen in the rest of the Península Ibérica. All different types of domes found in this place will be explained from its construction process, adding its structural capacity and energy bioclimatic qualities. -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- En el centro de Castilla y León todavía se mantienen algunos ejemplos de antiguas construcciones rurales vinculadas a las actividades económicas que se han desarrollado en el ámbito agrario. Éstas, conocidas como chozos y casetas, han sido ejecutadas con soluciones cupuliformes empleándose los materiales que más a mano se disponen, siendo el barro más característico en la región de Tierra de Campos y la piedra en la comarca de Montes Torozos. La influencia de las técnicas constructivas tradicionales de ambos elementos ha generado un abanico tipológico singular poco visto en el resto de la península. Los diferentes tipos de cúpulas encontrados se explicarán desde su proceso constructivo, añadiendo su capacidad estructural y sus cualidades bioclimáticas.

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The Canary Islands are mostly characterized by diffuse and scattered volcanism affecting a large area, with only one active stratovolcano, the Teide?Pico Viejo complex (Tenerife). More than 2 million people live and work in the 7,447 km2 of the archipelago, resulting in an average population density three times greater than the rest of Spain. This fact, together with the growth of exposure during the past 40 years, increases volcanic risk with respect previous eruptions, as witnessed during the recent 2011?2012 El Hierro submarine eruption. Therefore, in addition to purely scientific reasons there are economic and population-security reasons for developing and maintaining an efficient volcano monitoring system. In this scenario geodetic monitoring represents an important part of the monitoring system. We describe volcano geodetic monitoring research carried out in the Canary Islands and the results obtained. We consider for each epoch the two main existing constraints: the level of volcanic activity in the archipelago, and the limitations of the techniques available at the time. Theoretical and observational aspects are considered, as well as the implications for operational volcano surveillance. Current challenges of and future perspectives in geodetic volcano monitoring in the Canaries are also presented.

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The underground cellars that appear in different parts of Spain are part of an agricultural landscape dispersed, sometimes damaged, others at risk of disappearing. This paper studies the measurement and display of a group of wineries located in Atauta (Soria), in the Duero River corridor.

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Melon is traditionally cultivated in fertigated farmlands in the center of Spain with high inputs of water and N fertilizer. Excess N can have a negative impact, from the economic point of view, since it can diminish the production and quality of the fruit, from the environmental point of view, since it is a very mobile element in the soil and can contaminate groundwater. From health point of view, nitrate can be accumulated in fruit pulp, and, in addition, groundwater is the fundamental supply source of human populations. Best management practices are particularly necessary in this region as many zones have been declared vulnerable to NO3- pollution (Directive 91/676/CEE) During successive years, a melon crop (Cucumis melo L.) was grown under field conditions applying mineral and organic fertilizers under drip irrigation. Different doses of ammonium nitrate were used as well as compost derived from the wine-distillery industry which is relevant in this area. The present study reviews the most common N efficiency indexes under the different management options with a view to maximizing yield and minimizing N loss.

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Embankments constructed on hillsides can have serious problems of stability, generally created by the action of water com-bined with the inclination of the hillside. In order to increase the stability or correct problems of instability already present, there are various methods that can be used: surface and deep drainage, reinforcements with anchored beams, medium and large diameter piles, etc. Standing out among these systems (for its versatility) is the use of micropiles which ?sew? the embankment to a non-unstable area of the hillside. This paper presents research undertaken by means of a finite element code for studying the effect and stress of the micropiles, comparing the results with real measurements taken in the south of Spain.

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This study aimed to analyse several factors of variation of slurry composition and to establish prediction equations for potential methane (CH4) and ammonia (NH3) emissions. Seventy-nine feed and slurry samples were collected at two seasons (summer and winter) from commercial pig farms sited at two Spanish regions (Centre and Mediterranean). Nursery, growing-fattening, gestating and lactating facilities were sampled. Feed and slurry composition were determined, and potential CH4 and NH3 emissions measured at laboratory. Feed nutrient contents were used as covariates in the analysis. Near infrared reflectance spectroscopy (NIRS) was evaluated as a predicting tool for slurry composition and potential gaseous emissions. A wide variability was found both in feed and slurry composition. Mediterranean farms had a higher pH (p<0.001) and ash (p=0.02) concentration than those located at the Centre of Spain. Also, type of farm affected ether extract content of the slurry (p=0.02), with highest values obtained for the youngest animal facilities. Results suggested a buffer effect of dietary fibre on slurry pH and a direct relationship (p<0.05) with fibre constituents of manure. Dietary protein content did not affect slurry nitrogen content but decreased (p=0.003) total and volatile solids concentration. Prediction models of potential NH3 emissions (R2=0.89) and CH4 yield (R2=0.61) were obtained from slurry composition. Predictions from NIRS showed a high accuracy for most slurry constituents (R2>0.90) and similar accuracy of prediction of potential NH3 and CH4 emissions (R2=0.84 and 0.68, respectively) to models using slurry characteristics, which can be of interest to estimate emissions from commercial farms and establish mitigation strategies or optimize biogas production.

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In this paper, a hydroelectric power plant with long tail-race tunnel has been modelled for assessing its contribution to secondary regulation reserve. Cavitation problems, caused by the discharge conduit length, are expected downstream the turbine where low pressure appears during regulation manoeuvres. Therefore, governor's gains should be selected taking into account these phenomena. On the other hand, regulation services bidden by the plant operator should fulfil TSO (Transmission System Operator) quality requirements. A methodology for tuning governor PI gains is proposed and applied to a Hydro power plant in pre-design phase in northwest area of Spain. The PI gains adjustment proposed provides a proper plant response, according to some established indexes, while avoiding cavitation phenomena.

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Most red wines commercialized in the market use the malolactic fermentationprocess in order to ensure stability from a microbiological point of view. In this secondfermentation, malic acid is converted into L-lactic acid under controlled setups. Howeverthis process is not free from possible collateral effects that on some occasions produceoff-flavors, wine quality loss and human health problems. In warm viticulture regions suchas the south of Spain, the risk of suffering a deviation during the malolactic fermentationprocess increases due to the high must pH. This contributes to produce wines with highvolatile acidity and biogenic amine values. This manuscript develops a new red winemakingmethodology that consists of combining the use of two non-Saccharomyces yeast strains asan alternative to the traditional malolactic fermentation. In this method, malic acid is totallyconsumed by Schizosaccharomyces pombe, thus achieving the microbiological stabilizationobjective, while Lachancea thermotolerans produces lactic acid in order not to reduce andeven increase the acidity of wines produced from low acidity musts. This technique reducesthe risks inherent to the malolactic fermentation process when performed in warm regions.The result is more fruity wines that contain less acetic acid and biogenic amines than thetraditional controls that have undergone the classical malolactic fermentation.

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The Vicario Viaduct is located in the A-44 motorway, in the South-East part of Spain. It crosses a natural gorge, near the town of Ízbor in the province of Granada. A single continuous steel concrete composite deck, 24 m wide and 175 m long, divided in two 87,5 m spans, has been built. The cross section is a single structural steel box, 8,00 m wide and 4,52 m deep. The total width of 24 m is reached adding a strut and tie system each 4,375 m on both sides of the box. The steel parts of the deck were entirely constructed in the workshop and then they were erected on site just behind one of the abutments. Finally a 27.5 m long steel nose was connected to launch the deck. The main problems have been the curved shape of the deck (1420 m radius in plan) producing a non symmetric transverse distribution of reactions on each support and the cantilever reaching 87,5 m long, producing a maximum deflection of 1500 mm

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Purpose – The purpose of this paper is to describe the historical institutional context of Spain in the past 40 years and to analyze the influence of institutional factors in the current model of social enterprise existing in the country. Design/methodology/approach – This study draws on the theory of historical institutionalism, national-level empirical data and Kerlin conceptual framework (2013) that informs models of social enterprise. Findings – This paper describe some traits of Spain’s social enterprise that can be explained by the evolution of its institutional context in the past 40 years. It helps to validate, from a historical institutionalistic perspective, aspects of the Kerlin framework for social enterprise models. It also begins to show that the analysis of regional differences in the context should be taken into consideration when examining a country’s social enterprise space. Research limitations/implications – This discussion paper encourages academics to analyze regional differences in the emergence of social enterprise within a country. The main limitation of the paper is the lack of an “official” definition of social enterprise in Spain. Originality/value – This paper applies a valuable framework to a country with a unique political and economic history in the past 40 years. It contributes to enrich the research on the emergence and development of social enterprises in a variety of contexts and advances understanding of how regional differences inside a country influence the development of social enterprises.

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Airén is the most worldwide spread white grape cultivar, high yielding, well adapted to hot, dry conditions, and not very sensitive to fungal diseases. Its largest growing region is La Mancha, where Airén has been traditionally bush trained, spur pruned and grown with no irrigation. However, grape growing has evolved to meet the need for higher yields and harvest mechanization; and modern cultural practices train grape vines to simple multi-wire trellis systems, cane pruned, and usually irrigated. The aim of the present study was to evaluate the yield and sugar accumulating capacities of Airén cultivar with regard to leaf area, and to assess the influence that different yield components have on yield. In 2014, five commercial irrigated vineyards, located in La Mancha, of different ages, and grafted onto different rootstocks were selected for this study. Canopy surface area (SA) was measured at maturity. Berry weight and sugar concentration were measured during ripening on a weekly basis. Yield and yield components were determined at harvest. Values for shoot density ranged 2.3-5.1 shoots/m2; SA, 0.6-1.1 m2/m2; yield, 20-40 t/ha; fertility, 1.1-1.7 bunches/shoot; bunch weight, 450-650 g; berry weight, 2.5-2.9 g; and sugar concentration, 17-21 ºBrix. The number of bunches per shoot was the yield component that had the greatest influence on yield. The number of berries was the main contributing factor to bunch weight. A lineal relationship between SA/yield and sugar concentration was observed, with values of SA/yield ranging from 0.20 to 0.45 m2/kg. A ratio SA/yield of approximately 0.4 m2/kg was needed to reach a value of 20 ºBrix. Hence it would be necessary a SA of 12000 m2/ha, under the conditions of this study, to achieve a 30 t/ha yield, and a sugar concentration of 20 ºBrix. These results are a step forward in the study of the Airén cultivar, being of help for grape growers in the center area of Spain in order to maximize crop yield and sugar accumulation.

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Heparinase I from Flavobacterium heparinum has important uses for elucidating the complex sequence heterogeneity of heparin-like glycosaminoglycans (HLGAGs). Understanding the biological function of HLGAGs has been impaired by the limited methods for analysis of pure or mixed oligosaccharide fragments. Here, we use methodologies involving MS and capillary electrophoresis to investigate the sequence of events during heparinase I depolymerization of HLGAGs. In an initial step, heparinase I preferentially cleaves exolytically at the nonreducing terminal linkage of the HLGAG chain, although it also cleaves internal linkages at a detectable rate. In a second step, heparinase I has a strong preference for cleaving the same substrate molecule processively, i.e., to cleave the next site toward the reducing end of the HLGAG chain. Computer simulation showed that the experimental results presented here from analysis of oligosaccharide degradation were consistent with literature data for degradation of polymeric HLGAG by heparinase I. This study presents direct evidence for a predominantly exolytic and processive mechanism of depolymerization of HLGAG by heparinase I.

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NtrC (nitrogen regulatory protein C) is a bacterial enhancer-binding protein of 469 residues that activates transcription by σ54-holoenzyme. A region of its transcriptional activation (central) domain that is highly conserved among homologous activators of σ54-holoenzyme—residues 206–220—is essential for interaction with this RNA polymerase: it is required for contact with the polymerase and/or for coupling the energy from ATP hydrolysis to a change in the conformation of the polymerase that allows it to form transcriptionally productive open complexes. Several mutant NtrC proteins with amino acid substitutions in this region, including NtrCA216V and NtrCG219K, have normal ATPase activity but fail in transcriptional activation. We now report that other mutant forms carrying amino acid substitutions at these same positions, NtrCA216C and NtrCG219C, are capable of activating transcription when they are not bound to a DNA template (non-DNA-binding derivatives with an altered helix–turn–helix DNA-binding motif at the C terminus of the protein) but are unable to do so when they are bound to a DNA template, whether or not it carries a specific enhancer. Enhancer DNA remains a positive allosteric effector of ATP hydrolysis, as it is for wild-type NtrC but, surprisingly, appears to have become a negative allosteric effector for some aspect of interaction with σ54-holoenzyme. The conserved region in which these amino acid substitutions occur (206–220) is equivalent to the Switch I region of a large group of purine nucleotide-binding proteins. Interesting analogies can be drawn between the Switch I region of NtrC and that of p21ras.

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Transcription by RNA polymerase I in Saccharomyces cerevisiae requires a series of transcription factors that have been genetically and biochemically identified. In particular, the core factor (CF) and the upstream activation factor (UAF) have been shown in vitro to bind the core element and the upstream promoter element, respectively. We have analyzed in vivo the DNAse I footprinting of the 35S promoter in wild-type and mutant strains lacking one specific transcription factor at the time. In this way we were able to unambiguously attribute the protections by the CF and the UAF to their respective putative binding sites. In addition, we have found that in vivo a binding hierarchy exists, the UAF being necessary for CF binding. Because the CF footprinting is lost in mutants lacking a functional RNA polymerase I, we also conclude that the final step of preinitiation-complex assembly affects binding of the CF, stabilizing its contact with DNA. Thus, in vivo, the CF is recruited to the core element by the UAF and stabilized on DNA by the presence of a functional RNA polymerase I.