Sunshine Advisory, April 2003

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, August 2003

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, February 2003

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, December 2003

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, July 2003

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, February 2004

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Point Mutations in the Monocarboxylate Transporter SLC16A12 Lead to Juvenile and Age-Related Cataract

Purpose: Previously we reported on a premature termination mutation in SLC16A12 that leads to dominant juvenile cataract and renal glucosuria. To assess the mutation rate and genotype-phenotype correlations of SLC16A12 in juvenile or age-related forms of cataract, we performed a mutation screen in cataract patients. Methods: Clinical data of approximately 660 patients were collected, genomic DNA was isolated and analyzed. Exons 3 to 8 including flanking intron sequences of SLC16A12 were PCR amplified and DNA sequence was determined. Selected mutations were tested by cell culture assays, in silico analysis and RT-PCR. Results: We found sequence alterations at a rate of approximately 1/75 patients. None of them was found in 360 control alleles. Alterations affect splice site and regulatory region but most mutations caused an amino acid substitution. The majority of the coding region mutations maps to trans-membrane domains. One mutation located to the 5'UTR. It affects translational efficiency of SLC16A12. In addition, we identified a cataract-predisposing SNP in the non-coding region that causes allele-specific splicing of the 5'UTR region. Conclusions: Altered translational efficiency of the solute carrier SLC16A12 and its allele-specific splicing strongly support a model of challenged homeostasis to cause various forms of cataract. In addition, the pathogenic property of the here reported sequence alterations is supported by the lack of known sequence variations within the coding region of SLC16A12. Due to the relatively high mutation rate, we suggest to include SLC16A12 in diagnostic cataract screening. Generally, our data recommend the assessment of regulatory sequences for diagnostic purposes.

Sunshine Advisory, May 2004

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, March 2004

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, July 2004

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, June 2004

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Juvenile Crime and the Welfare of Youth, 1999

This staff report on various factors regarding juvenile issues and crime was prepared by the Division of Criminal and Juvenile Justice Planning (CJJP). It was prepared to help the Juvenile Justice Advisory Council (JJAC) prepare their 3-Year Strategy as prescribed by the Juvenile Justice and Delinquency Prevention Act (JJDP Act).

Breast sarcomas: current and future perspectives.

Breast sarcomas are rare neoplasms of the breast that need to be clearly distinguished from the very common breast carcinomas and treated in a multidisciplinary manner modelled after treatment paradigms in other sarcoma locations. An increasing need to differentiate sarcoma sub-types based on molecular characteristics that will also be depicted in differential treatment sensitivities and development of specifically targeted therapies are equally valid in sarcomas in general and in breast sarcomas in particular. Of special interest in breast are sarcomas developing after breast irradiation for a previous breast carcinoma, a scenario that is increasingly common, given the increasing trends of breast conservation in the surgical treatment of breast carcinoma that necessitates the adjuvant use of radiotherapy.

Sunshine Advisory, August 2004

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller

Sunshine Advisory, September 2004

A Bulletin on Iowa Open Meetings and Public Records Laws By Attorney General Tom Miller