Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

The present study evaluated the use of PCR for Histophilus somni detection in bovine semen. Semen samples were experimentally infected with H. somni at dilutions ranging from 107 to 101 bacteria/mL and subjected to DNA extraction by the phenol/chloroform method, followed by PCR amplification. The amplification products were analyzed by electrophoresis in 8% acrylamide gel. The oligonucleotide primers used yielded an amplification fragment of 400 base pairs from the bacterial DNA. Positive amplification was obtained even for the 101 bacteria/mL dilution. PCR proved to be an efficient method for the detection of H. somni. The results obtained in this study have brought relevant information for the diagnosis of H. somni, justifying the need for the diagnosis of this bacterium in bulls, especially in semen samples that should be free of contamination. The PCR method has shown to be a useful tool for the quality control of semen produced in artificial insemination centers.

Narrativa audiovisual complexa: modular narratives e database narratives

Procura-se nesse trabalho expor as considerações levantadas sobre a modularidade e a temporalidade do filme Pulp Fiction (1994), de Quentin Tarantino, enquadrada por Simons (2008) nas categorias de narrativa audiovisual complexa denominadas modular narratives e database narratives. Com Ricoeur (1983), discutiu-se a sua organização temporal a partir do conceito de tríplice presente em Santo Agostinho, enquanto com Propp (1970) e Cameron (2008) o cará- ter modular dessa narrativa.

Relacionamento e coordenação da cadeia produtiva da mandioca no Noroeste do Paraná

The production of starch and cassava starch (Manihot esculenta) if has shown to an important business for the region the northwest of the Paraná, generating income and job for a significant agricultural producers. The production of the region is processed for industries at private capital and cooperatives of producers. The study involving agricultural producers and one of the most important cooperatives of the region (C-Vale) objectified to evaluate the relationships between cooperative and producers, and the mechanisms of coordination of the productive chain. The results had disclosed that the relationships are not harmonious, generating opportunism situations, high costs of transaction and diffidence in the operations between producers and cooperative. Also if it perceived a difficulty of coordination of the productive chain, due to lack of integration between the partners, what it affects the competitiveness and the efficiency of the sector.

Educação Tutorial como ferramenta no Ensino-Aprendizagem de Audiovisual

This paper aims to highlight the importance of Tutorial Education Program (TEP) for undergraduate courses in conjunction with the graduate programs, highlighting the relevance of the triad teaching, research and extension activities also present in the TEP Interdisciplinary in Radio and Television from the FAAC/UNESP.

Jornalismo audiovisual: da tela da TV para outras telas

This article is based on research which has been developed in partnership with Unesp TV, a university TV broadcast station of the Universidade Estadual Paulista Julio de Mesquita Filho, Bauru campus/SP. The study aims to identify convergent and divergent aspects in the design of audiovisual journalistic content for TV and other media such as the internet and mobile communication systems. The results presented here are the considerations obtained from the first stage of the research. In this phase, the basic steps which should guide the design of the content to feed broadcasting time are outlined, as well as the online audiovisual news broadcast and business management of a TV station, compared to the model which has been followed by internet TV broadcasters.

Para além da fidelidade na adaptação audiovisual: o caso da minissérie televisiva Capitu

Despite the theoretical advances that have discredited the notion of “faithfulness” regarding the adaptation as an intermediatic phenomenon, it still insists on coming up. Capitu, a mini-series shown by the Rede Globo network has been regarded as “unfaithful” to the novel Dom Casmurro. It is a perfect example for this article of the perception of the audiovisual language resources that work as valid analogies to Machado de Assis’ novel, indicating the misconception that underlies the notion of “faithfulness”.

A iteratividade: do melodrama à narrativa audiovisual sob demanda

The repetition should be understood as the property of mass cultural products, originated from the need to minimize the dispersion of the audience in their enjoyment. Based on this assumption, this paper measures the rate of iteration manifested in the scripts of the following serial fictions: Duas Caras, A Grande Família and House of Cards. Established itself as a method of scene analysis proposed by McKee (2008), questioned whether the lack of classical education of the public and rescued the forms assumed by repeating the melodrama, romance-serial and dramaturgy: asides, monologues, confidences and planar scenes. The proposal was applied in two scripts for each of the aforementioned productions. Considering the data collected, it appears that there was reduction in repeat products offered on demand thanks to the possibility of handling the flow and the attention given receptor.

Quantificação de enterobactérias e Clostridium spp. e detecção molecular de Clostridium perfringens, Escherichia coli e Salmonella spp. em pontos de cadeia produtiva da carne de frango

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise das práticas de gestão da cadeia de suprimentos em empresas calçadistas da cidade de Jaú - SP

Pós-graduação em Engenharia de Produção - FEB

Diagnóstico laboratorial de Mycobacterium spp. em Botucatu e região, utilizando a técnica Reação em Cadeia da Polimerase (PCR) em material biológico e avaliação de condições associadas

Pós-graduação em Saúde Coletiva - FMB

Narrativas interativas em audiovisual panorâmico para dispositivos móveis

Pós-graduação em Comunicação - FAAC

Redução do MELD em paciente em lista de transplante hepático após o uso de aminoácidos de cadeia ramificada: relato de caso

Liver cirrhosis (LC) is a disease with high mortality rates and its only definitive treatment is the orthotopic liver transplantation (OLT). Unfortunately, not all patients have access to OLT and many of them end up dying on the transplant waiting list. The use of branched chain amino acids (BCAA) is widely known as an effective treatment for improving the quality of life of these patients. For the first time, in this paper we documented a great improvement of clinical and laboratorial tests of a patient treated with BCAA, which allowed him to be out of the transplant waiting list. In addition to the increase of the MELD score, the patient achieved restabilization of body weight and recovery of the quality of life registered by the SF-36 questionnaire.

Detecção de Brucella spp. em leite bovino não pasteurizado através da Reação de Cadeia pela Polimerase (PCR)

Brucellosis is a zoonosis caused by bacteria of the genus Brucella. Man infection occurs through contact with reproductive secretions as placenta and its lochia, semen and penile secretion of infected animals or by consuming unpasteurized milk and dairy products. With the objective of investigating the presence of bacteria in milk, 30 samples of raw milk sold illegally in the region of Botucatu, São Paulo, Brazil, as well as 50 samples of milk delivered to a dairy industry previously to its pasteurization were evaluated by the polymerase chain reaction (PCR) technique. Of the 80 samples analyzed, 10 samples (12.5%) were positive and 70 (87.5%) were negative. Among the  positive samples,  5 (16.6%)  were from  illegal traders  and other  5  (10%) were obtained  from the dairy industry. Brucella spp. positivity shows that the pathogen is representatively present in Botucatu, São Paulo, Brazil, and the risk associated to public health due to the commercialization of illegal products without pasteurization is real.

Comparação de métodos para extração de dna na reação em cadeia da polimerase para detecção de Salmonella enterica sorovar enteritidis em produtos avícolas

The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances present in samples may affect the reaction. The present work aimed to compare DNA extraction by thermic treatment and by the use of cetyltrimethil ammonium bromide (CTAB), in products originated from poultry houses corresponding to raw material (meat meal) and experimentally contaminated drag swabs. Materials obtained from the extractions were submitted to PCR, utilizing a pair of initiator oligonucleotides for amplification of Sdf 1 gene fragments. Comparing the methods of extraction, it was observed that when CTAB was employed, SE was detected in 70% of meat meal and in 80% of drag swabs, while the thermic treatment method yielded positive results in 20% of meat meal and in 40% of drag swabs. SE was detected under both methods utilized for DNA extraction, but the use of CTAB detected a greater number of positive samples, compared with thermal treatment.

Avaliação das alterações cardiopulmonares precoces e tardias resultantes da secção da cadeia simpática realizada por videotoracoscopia em pacientes com hiperidrose

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)