Complete protection against P. berghei malaria upon heterologous prime/boost immunization against circumsporozoite protein employing Salmonella type III secretion system and Bordetella adenylate cyclase toxoid

Sterile immunity against malaria can be achieved by the induction of IFNgamma-producing CD8(+) T cells that target infected hepatocytes presenting epitopes of the circumsporozoite protein (CSP). In the present study we evaluate the protective efficacy of a heterologous prime/boost immunization protocol based on the delivery of the CD8(+) epitope of Plasmodium berghei CSP into the MHC class I presentation pathway, by either a type III secretion system of live recombinant Salmonella and/or by direct translocation of a recombinant Bordetella adenylate cyclase toxoid fusion (ACT-CSP) into the cytosol of professional antigen-presenting cells (APCs). A single intraperitoneal application of the recombinant ACT-CSP toxoid, as well as a single oral immunization with the Salmonella vaccine, induced a specific CD8(+) T cell response, which however conferred only a partial protection on mice against a subsequent sporozoite challenge. In contrast, a heterologous prime/boost vaccination with the live Salmonella followed by ACT-CSP led to a significant enhancement of the CSP-specific T cell response and induced complete protection in all vaccinated mice.

The complete works of Count Rumford /

Green cloth binding, with gilt spine titles.

Complete songs and poems of Robert Tannahill, with life and notes.

Includes index.

The complete works of Samuel Rowlands, 1598-1628, now first collected ...

v.3. Heavens glory, seeke it, &c., 1628. The famous history of Gvy erle of Warwicke, 1682. Miscellaneous poems. Notes, by Sidney J.H. Herrtage. Glossary, by Sidney J.H. Herrtage.

Report of the commissioners of inland revenue on the duties under their management, for the years 1856 to 1869 inclusive; with some retrospective history, and complete tables of accounts of the duties from their first imposition ... Presented to both Houses of Parliament by command of Her Majesty.

Mode of access: Internet.

Handbook of the law of Mexican commercial corporations, including foreign corporations in Mexico (in English) containing explanations of the system of laws of the republic of Mexico and the law of the contract, management and operations of general corporations organized in Mexico; as well as of foreign corporations operating therein; together with complete forms for use by lawyers and corporate officials,

Mode of access: Internet.

The royal atlas of England and Wales, reduced from the ordnance survey; a complete series of topographical maps, physical and statistical charts, town plans, and index of 35,000 names ..

Mode of access: Internet.

The farmer's and emigrant's hand-book : being a full and complete guide for the farmer and the emigrant [...] /

Mode of access: Internet.

Farm knowledge; a complete manual of successful farming written by recognized authorities in all parts of the country; based on sound principles and the actual experience of real farmers - "The farmer's own cyclopedia",

The volumes are issued in two parts each, with individual t.-p.

Leadless decorative tiles, faience, and mosaic, comprising notes and excerpts on the history, materials, manufacture & use of ornamental flooring tiles, ceramic mosaic, and decorative tiles and faience, with complete series of recipes for tile-bodies, and for leadless glazes and art-tile enamels,

Mode of access: Internet.

A complete treatise on the art of retouching photographic negatives and clear directions how to finish & colour photographs.

Mode of access: Internet.

The Modern world dictionary of the English language ... to which is added a compendium of historical, biographical, geographical, scientific, religious, and sociological names, and a complete summary of human achievement in the nineteenth & twentieth centuries ...

Editors-in-chief: Robert Hunter and Charles Morris.

The complete works of Michael Drayton, now first collected.

With reproductions of original title-pages.