Descripción inmunohistoquímica de la encefalitis en bovinos brasileños naturalmente infectados con el herpesvirus bovino tipo 5 (HVB-5)

Twelve cases of viral meningoencephalitis in Brazilian cattle were submitted to immunohistochemical analysis for inflammatory response description. All the cases showed severe neurological signs followed by death. Mild to moderate histological inflammatory changes in the brain and cerebellum characterized the neurological infection showing meningitis, mononuclear perivascular cuffing, gliosis, haemorrhage, and macrophages (gitter cells) accompanying great areas of malacia. None of the cases showed intranuclear inclusion bodies. However, in five of them it was possible to isolate the BoHV-5. In order to collect data to allow the description of the inflammatory response in these cases, brain samples from all of the cases were analyzed by immunohistochemistry using polyclonal antibodies against CD3 to detect T cells, and against GFAP to detect astrocytes. On the other hand, monoclonal antibodies were used against BLA to detect B cells and, against MAC 387 to detect macrophages. The results indicate different degrees of prominent astrocytic response, and at the same time, T lymphocytes constituted a high percentage of the mononuclear cells which characterized the inflammatory response.

Interacción genotipo x ambiente sobre el peso al destete de bovinos nelore en Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Selenium supplementation enhances weight gain in cattle

To evaluate the effect of selenium on cattle growth in a continuous grazed pasture system. Selenium was added to protein-mineral salt and given to male, around-12-month old, non-castrated Nellore calves. Animals were randomly and equally distributed into 4 groups (15 calves/group) which received supplementation containing 0, 3.6, 5.4 or 6.4 mg selenium/animal/day (groups Gc, G 3.6, G 5.4 and G 6.4, respectively). The animals were weighed on day zero and 120, and the pasture forages were collected at day 0 for chemical analyses. Weight gain was higher in cattle from G 5,4 (45.58%) than in the other groups, and higher in G 3,6 (24.97%) and G 6,4 (22.67%) than in Gc. The supplementation with 5.4 mg selenium/animal/day enhanced weight gain in cattle fed on selenium poor diet (0.04 mg Se/kg dry matter).

Modelación para ajuste de la curva de lactación usando función lineal, no lineal y polinomios segmentados en ganados con alto grado de sangre Brown Swiss

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Profitability measures of dairy cows

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise histopatológica de órgãos linfóides de bovinos e camundongos intoxicados experimentalmente por Baccharis coridifolia: Caracterização imunoistoquímica de linfócitos B e T

Baccharis coridifolia is one of the most important poisonous plants to cattle in the South of Brazil. The plant provokes necrotic lesions in the lymphoid tissues and in the gastrointestinal tract of cattle. Experimental administration to mice produces most of the lesions seen in the lymphoid tissues of cattle. This study was conducted to search possible differences in the susceptibility of T and B lymphocyte subpopulations. Lymph nodes, spleen, thymus and gut-associated lymphoid tissue (GALT) of cattle and mice experimentally poisoned were evaluated. The results were evaluated based on cell populations affected or remaining in the organs. Immunostaining for B lymphocytes (anti-BLA-36) identified the germinal center of follicles of the lymph node, spleen and GALT in both species. Immunostaining for T lymphocyte (anti-CD3) identified the paracortical area of the germinal centers of the lymph nodes and GALT, the periarteriolar area of the spleen, and the whole thymus both in cattle and mice. Experimentally poisoned cattle and mice shows necrosis of the germinal center of secondary follicles of the lymph nodes, spleen and GALT, where necrotic cells were immunostained for B and less often for T lymphocyte. Necrotic cells in the paracortical region of the lymph node were less often and were immunostained. Necrotic lesions of the thymus were seen only in mice, with positively stained for T lymphocyte. The distribution of the lesions in the lymphoid tissues and the immunostaining in necrotic cells suggested that the active principles of the plant are cytotoxic to B and T cells.

Propriedades químicas de um latossolo vermelho de cerrado cultivado com algodão sob diferentes preparos e adubações

The high rate of mineralization of organic matter on savannah soils, which is reached fi ve times faster than in temperate regions, leads us to the challenge of electing the best system of management that maintains and/or increase it in soil, guaranteeing its quality and sustainability. In this sense the present research aimed to study the effects of green, organic and mineral manure on the chemical properties on the chemical properties of an Oxisol, on Savannah area, cultivated with cotton (Gossypium hirsutum) under conventional tillage and no-tillage in the pasture fi eld (Brachiaria decumbens) for 20 years. The experimental design was a randomized block design with split plots. The main plots consisted of two treatments: conventional tillage and no-tillage, and the subplots of six treatments: control (no fertilizer), mineral fertilizer recommended for the crop, according to the soil chemical analysis, organic fertilizer (cattle manure - 20 t ha-1), organic fertilizer (cattle manure - 20 t ha-1) + 1/2mineral fertilization recommended according to the analysis of soil, green manure-1 (Crotalaria juncea) and green manure-2 (Pennisetum americanum). There were studied the following soil chemical properties: P, OM, pH, K, Ca, Mg, Al, Al + H, S, exchange capacity cations and base saturation. The soil samples for the analysis were performed on layers of 0,00-0,05 m, 0,05-0,10 and 0,10-0,20 m. Then it came the following conclusions: the fertilization interfere in soil chemical properties and the preparation did not interfere, the cattle manure and its association with the mineral fertilizer caused increasing level elements in the soil, Crotalaria juncea and Pennisetum americanum did not infl uence on soil chemical properties.

A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Carrying capacity in agriculture: Environmental significance and some related patents

Agriculture is one of the most important and possibly the oldest economic activity developed by humans. This activity was developed extensively and is becoming more and more dependent on development of technologies. The goal of this manuscript was examining some patents related to technologies developed for improving crop yields. Such patents are mainly related to more efficient formulations of agrochemicals and management techniques of plants, cattle and natural resources. A brief comment is carried out about bioprospection and related problems, relating, for example the case of Cupuaçu. The article is concluded mentioning that the development of policies and management strategies that increase agricultural yield and simultaneously preserve or conserve natural resources should also be prioritized, because certainly this is the only way we have to get the real sustainability and to improve life quality abroad the world.

Inferência bayesiana na predição de valores genéticos do peso aos 365 dias de bovinos de corte

Data from purebred Simmental, Nellore and Canchim cattle breeds obtained from the respective Brazilian Associations of Breeders were used to estimate variance components and to predict genetic values for 365 days weight. The results obtained by Bayesian inference were compared to those from Restricted Maximum Likelihood (REML) and Best Linear Unbiased Prediction (BLUP), which are the most commonly used methods of estimation and prediction in animal breeding. The two methods presented similar point estimates but the study of the marginal posterior distributions in the Bayesian approach yields more detailed information about the parameters and other unknowns in the model.

Consumo de capim-elefante com diferentes idades fornecido para vacas leiteiras

The 30, 45 and 60 day-old elephant grasses were evaluated by monitoring their nutritional profile and DM (DMI) and NDF (NDFI) intake by crossbred lactating cows in a 3 × 3 Latin square trial, where the split-plots were the rumen evacuation times: 0, 2, 4 and 6 h after meal. Chemical composition, in vitro dry matter digestibility and ruminal content ranged with grass age. Means (in kg/cow/day) of DMI and NDFI of 30 day-old grass (8.0 and 5.3) were lower than those of grasses with 45 days (10.0 and 6.6) and 60 days (11.0 and 7.3). The maximum of rumen fill was observed at 4:22, 3:55 and 3:49 hours after feeding for treatments with 30, 45 and 60 day-old elephant grass, respectively. Rumen fill did not limit the DMI and NDFI of elephant grass, but the dry matter intake of 30 day-old grass may be affected by the wet content.

Manejo pré-abate de bovinos de corte no Pantanal, Brasil

This study has the objective of evaluate the influence of fluvial and road transport on the occurrence of bruising in carcass of slaughtered beef cattle. In this study was observed that from 209 evaluated carcass, 185 (88.5%) presented one or more lesions. In total of evaluated carcass occurred 523 lesions that resulted in 96.158 kg removal of bruises (0.519 kg per animal). In this study was possible the determination of age of bruises by color evaluation. We concluded that of way of transport influenced the occurrence of bruises. The most bruises occurred in the last 24 hours of transport. Educational measures will be necessary to reduce the problems of pre-slaughter management.

Correlações entre morfometria ovariana e hormônios em vacas zebus não prenhes

The study of ovarian morphometry is directly related to its practical applications to interpret the examination of the reproductive tract of the cow. The objective of this work was to study the ovarian morphometry collected in slaughterhouse and correlations between corpus luteum area, progesterone and cortisol in no pregnant zebu cows. One hundred fourteen pairs of ovaries were collected from slaughterhouse, measured in thickness, length, width and volume, diameter and volume of the follicle, diameter and area of the corpus luteum. Significative difference (p<0.05) were observed for widths of (1.95 cm and 1.83 cm) and volume (p<0.05) of 7.26 ml and 6.23 ml of left and right ovaries respectively. For size and volume of follicles, and diameter and area of the corpus luteum, there was no significative difference between the sides. There was correlation of 0.46 (p<0.01) between left ovary volume × luteum corpus area, in the presence of follicles with diameters equal or greater than 9 mm, the corpus luteum of the massive and protuberant type were present in 23 (43.39%) of 53 ovaries, predominating in relation to type of massive and incluse 16 (30.18%). There were no significative correlation between corpus luteum area × progesterone, corpus luteum area × cortisol and progesterone × cortisol. In conclusion, the presence of the included corpus luteum in zebu cows could result in error during the rectal palpationexam to estimate the ovarian activity.

Irrigação de pastagens via pivô central, na bovinocultura de corte

The aims of this work were to show the pasture irrigation system by center pivot with Brazilian cattle and to discuss the economic feasibility of this technique in different regions of the country. Important parameters to dry matter production of tropical forage plants, as temperature, solar radiation, fertilization, and water requirement were shown Also, the system advantages and disadvantages and a discussion about economic feasibility of this technique were presented. It was concluded that pasture irrigation is a feasible and economical technique to some specific Brazilian regions, depending on appropriated parameters. This work also concludes that just water supply is not enough to assure forage production avoiding reduction in dry production in the winter.

Relações materno-filiais e sua influência no peso pré-desmama de animais Nelore da Bahia

The aim of this study was to investigate the maternal-offspring relationships in the bovine breeds Nellore PO (Pure Breed) and Nellore CL (Cara Limpa) in Bahia State. The genetic composition of breeds did not affect LP (latency time to stand); this variable showed mean values of 71 min and 52 min, respectively, for Nellore PO and Nellore CL. The variable LPTM (latency time to begin suckling after standing) was significantly higher for Nellore PO (mean values of 39 min) tham for Nellore CL (18 min). The variable TCC (time in contact with the calf) was classified as class 4 (60 to 80% of the time was spent with the calves). The P120 (weight at 120 days) had mean values of 122.3 kg and 122.7 kg respectively, for Nellore PO and Nellore CL. In conclusion, the maternal-offspring behaviour had little influence of the genetic composition of the breeds. These results suggest that more studies are necessary to define technological parameters for cattle raising in Bahia State.