A single multiplex SNaPshot reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups

SNaPshot minisequencing reaction is in increasing use because of its fast detection of many polymorphisms in a single assay. In this work we described a highly sensitive single nucleotide polymorphisms (SNPs) typing method with detection of 42 mitochondrial DNA (mtDNA) SNPs in a single PCR and SNaPshot multiplex reaction in order to allow haplogroup classification in Latin American admixture population. We validated the panel typing 160 Brazilian individuals. DNA was extracted from blood spotted on filter paper using Chelex protocol. Forty SNPs were selected targeting haplogroup-specific mutations in Europeans, Africans and Asians (only precursors of Native Americans haplogroups A2, B2, C1, and D1) and two non-coding SNPs were chosen to increase the power of discrimination between individuals (SNPs positions 16,519 and 16,362). It was done using a modified version of a previously published multiplex SNaPshot minisequencing reaction established to resolve European haplogroups, adding SNPs targeting Africans (L0, L1, L2, L3, and L*) and Asians (A, B, C, and D) haplogroups based on SNPs described at PhyloTree.org build 2. PCR primers were designed using PerlPrimer software and checked with the Autodimer program. Thirty-three primer-pairs were used to amplify 42 SNPs. Using this panel, we were able to successfully classify 160 individuals into their correct haplogroups. Complete SNP profiles were obtained from 10. pg of total DNA. We conclude that it is possible to build and genotype more than 40 mtDNA SNPs in a single multiplex PCR and SNaPshot reaction, with sensitivity and reliability, resolving haplogroup classification in admixture populations. © 2011.

Características morfoagronômicas do milho submetido a diferentes níveis de desfolha manual

The present study was conducted to evaluate the damage caused by defoliation in maize. The experiment was carried out in 2005/2006 at Roma Farm, Ituverava-SP, using the triple hybrid CODETEC 304®. The experiment consisted of split plots (split-plot), which were distributed at random in three blocks, with the main treatment manual defoliation (30%, 40%, 60% and 80% defoliation) in the plots and as the secondary treatment the phenological phases (D1, D2 and D3) with three replicates for each treatment and a control plot (0% defoliation) for each block. It was evaluated the production of plots (g/m2), the average size of the spikes (cm) and the average weight of thousand grains (g). According to the results, it was concluded that the source-sink relations were negatively affected, and the treatment with 80% defoliation was the one that most affected all variables, thus, it is a level of defoliation that is responsible for the largest losses, with no means of compensation for the maize crop.

Intraspecific variation and emendation of Hannaella kunmingensis

In order to investigate the intraspecific variability in Hannaella kunmingensis, 11 isolates, including the type strain, were analyzed for their morphological and biochemical traits. The combined internal transcribed spacer region (ITS), D1/D2 domains of the large subunit rDNA (LSU), and cytochrome b gene were examined using phylogenetic and parsimony network analyses. Our investigations revealed differences in colony morphology as well as differences in 31 out of 64 phenotypic characteristics examined, including growth in lactose, vitamin free medium, xylitol, L-arabinitol, and nitrite. Growth in the presence of 0. 1 % cycloheximide was also highlighted in H. kunmingensis. All the 11 strains were conspecific in the LSU; however, variations of about 2. 5 % were found in the ITS while isolate CBS 8356 exhibited a 27. 3 % divergence from the other strains in the cytochrome b gene. Parsimony network analysis revealed the existence of three haplotypes among the H. kunmingensis strains studied but excluded CBS 8356 from the network connecting these haplotypes. This study contributes to the knowledge of the intraspecific diversity of H. kunmingensis. To accommodate such intraspecific variations, an emendation of the species diagnosis is proposed. © 2012 German Mycological Society and Springer.

Ultrassonografia oftálmica em cães com diferentes conformações cranianas

The aim of this study was to establish mean values for intraocular structures and analyse if the differences are present for different skull conformations in dogs. In this study, 30 dogs were selected and distributed into three groups according to skull conformation; thus, group 1 (G1) was composed of brachycephalic dogs, group 2 (G2) was composed of mesocephalic dogs and group 3 (G3) was composed of dolichocephalic dogs. A and B-mode ultrasound was performed simultaneously for obtainment of measurements relating to anterior chamber depth (D1); lens thickness (D2); vitreous chamber depth (D3); and the axial length of the eye (D4). No differences were observed when comparing left and right eyes of dogs within the same skull conformation group (p>0.05). Differences were observed when comparing D3 and D4 between groups G2 and G3 (p<0.05). Skull conformation of brachycephalic dogs did not influence intraocular measurement values when compared to dolicephalic and mesocephalic dogs. Skull conformation of dolichocephalic dogs had an influence in values of vitreous chamber and the complete length of the eye when compared to mesocephalic dogs.

Stocking density of Nile tilapia in cages placed in a hydroelectric reservoir

The Brazilian government has been encouraging fish farming in cages in federal water bodies, including hydroelectric reservoirs. Despite the government support, it is a new activity and the production model still needs some adjustment to reduce the production costs and achieve sustainability. The aims of this study were to determine the appropriate stocking density of Nile tilapia in cages in a hydroelectric reservoir and to evaluate to what extent fish size selection could improve their uniformity. Twelve cages (6m3) were placed at the Fish Farmers' Cooperative of Santa Fé do Sul and Region, Ilha Solteira reservoir, São Paulo, Brazil (20°12'10″S, 50°58'31.15″W). In stage I (initial fish weight, 78g), four stocking densities were tested: D1-800, D2-2000, D3-2500 and D4-3000 fish/cage, with three replicates. At the end of this stage (average fish weight, 255g), the fish were selected into three sizes, except for D1. In stage II, four stocking densities were tested, designed to obtain the following final production: D1-100kg/m3 (800 non-selected fish/cage), D2-80kg/m3 (600 fish/cage), D3-100kg/m3 (800 fish/cage) and D4-120kg/m3 (900 fish/cage). The trial ended when the fish weighed 800g. By reducing the initial stocking density from 2500 to 800 tilapia juveniles per cage, there was no need for selection. The growth performance was higher, the feed conversion rate was better and the time taken to reach harvesting was shorter. Consequently, the production cost reduced and the operating profit increased. Using the lowest initial stocking density, the risk of disease outbreak was also lower, and there was no need to use drugs for disease control since the mortality rate and occurrences of disease and deformity decreased and the dissolved oxygen level inside the cages was higher. © 2013 Elsevier B.V.

Consórcios microbianos associados a ambientes de minas: obtenção, avaliação fisiológica e molecular

Pós-graduação em Biotecnologia - IQ

Avaliação da resposta à quimiorradioterapia neoadjuvante em pacientes com adenocarcinomas retais

Pós-graduação em Bases Gerais da Cirurgia - FMB

Isolamento de leveduras fermentadoras de pentoses e suas aplicações na produção de xilitol e etanol a partir do licor negro proveniente do processo Kraft de extração da celulose

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Liberação das enzimas LDH e PepX e evolução da maturação de queijo parmesão adicionado de culturas autolíticas de Lactobacilus helveticus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da qualidade industrial de linhagens de trigo por meio de métodos físico-químicos, reológicos e de panificação

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Desempenho de métodos baseados na indução de fluorescência para a detecção de lesões de cárie em superfícies oclusais e proximais: estudos in vitro

Pós-graduação em Ciências Odontológicas - FOAR

Ultrassonografia do bulbo do olho de cães, com diferentes conformações cranianas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo da segurança da dose terapêutica oral do firocoxib em equinos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise econômica da aplicação de biossólido na agricultura

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comportamento assintótico dos polinômios ortogonais de Sobolev-Jacobi e Sobolev-Laguerre

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)