Efeito de parâmetros variáveis de fermentação sobre o crescimento celular e composição química do fermentado de batata-doce

This study aimed to evaluate the use of sweet potato as a substrate for the production of spirits. In order to promote an alternative technologically feasible, an experimental design was performed to minimize the operations of preparation, defining the most adequate conditions for the fermentation process. From sweet potato flour obtained by milling and dehydration process of the roots was carried out an enzymatic process of hydrolysis-saccharification of suspension of sweet potato flour with 18% dry matter. The hydrolyzate was used in the fermentation process which followed the 23 full factorial experimental design with central and axial points, and the independent variables were the concentration of reducing sugars, concentration of viable yeast and fermentation temperature. The dependent variables were viable cells, residual sugar, ethanol, glycerol and methanol. The dependent variables were quantified by liquid chromatography. The data analysis indicate that the best fermentation conditions among the tested conditions were: concentration of yeast 5 x 107-1 x 108 in number of viable cells, total reducing sugars from 12.5 to 13.5% and temperature between 33 -34ºC.

Recuperação de amidos remanescentes em resíduos de extração da fécula de mandioca

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fermentação alcoólica com Saccharomyces cerevisiae em mosto aerado de hidrolisados de amido de mandioca

O presente trabalho foi desenvolvido no Centro de Raízes e Amidos Tropicais – CERAT, Na UNESP em Botucatu, estado de São Paulo onde foram realizados ensaios de fermentação alcoólica com hidrolisado de amido de mandioca. A fécula de mandioca foi utilizada como fonte de carboidrato para obtenção dos açúcares redutores consumidos no processo. Em um reator agitado foi produzido 12Kg de hidrolisado a partir de suspensão de fécula a 30% (p/p) utilizando enzimas alfa amilase na primeira etapa, seguida de amiloglucosidase na etapa posterior. As dosagens em unidades enzimáticas foram 2KNU.g-1 de amido e 2AGU.g-1 de amido respectivamente. O planejamento experimental considerou a realização de três ensaios de hidrolisados e três ensaios de fermentação a partir do mosto produzido; a) mosto aerado; b) com microaeração; c) em meio anaeróbio. Os ensaios foram realizados em erlenmeyers com 2,5 Kg de hidrolisado, ajustado a concentração de glicose a 100g.L-1 sendo inoculada a levedura do gênero Saccharomyces cerevisiae à taxa de 1,5% (p/p). Todos os erlenmeyers foram colocados sob agitação orbital e temperatura controlada de 30ºC sendo acompanhado o processo de fermentação através de coleta de amostras do mosto a cada hora. A aeração nos frascos erlenmeyers foi realizada através de mangueira coletora de válvula que regulava a vazão de ar. De acordo com os dados obtidos pode se concluir que o sistema anaeróbio em 32h foi o mais eficiente para a produção de etanol. Também foi possível observar que enquanto ocorre aeração no meio não se observa alteração significativa na concentração de etanol e quando cessa a aeração o meio torna se anaeróbio e tem início a produção de etanol. Quando aumenta a concentração de etanol no meio, o crescimento celular do sistema anaeróbio cai e etanol inibe a levedura, parando o crescimento celular.

Prospecção de genes na microbiota do rúmen bovino com propriedades degradadoras da biomassa vegetal

Pós-graduação em Microbiologia Agropecuária - FCAV

Adubação potássica na produção e qualidade pós-colheita do rabanete

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção e qualidade de batata-doce em função das doses e parcelamento da adubação potássica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Purificação parcial e caracterização bioquímica de uma isoforma de β-glicosidase do fungo termofílico Myceliophthora thermophila M.7.7

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ação do ácido abscísico na cor das bagas, na qualidade e no armazenamento pós-colheita da uva Rubi

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção de etanol utilizando cascas de banana e de laranja por co-fermentação de Zymomonas mobilis e Pichia stipitis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Energetic feedings influence beeswax production by Apis mellifera L. honeybees

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise isotópica do carbono em néctares de caju

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito de reguladores vegetais na qualidade de frutos de melão rendilhado

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Composição química e propriedades de pasta de farinhas de mandioca

This work aimed to analyze the chemical composition and paste properties of cassava flours, from several producers and classifications, marked in different Brazilian cities. Flours were characterized to moisture, ash, fibers, protein, lipids, total sugars and starch. The paste properties were analyzed in RVA. The results showed significant differences to chemical components in cassava flours. The moisture ranged from 4.39 to 10.26, starch (82.19 to 88.90%), ash (0.48 to 1.07%), fiber (3.23 to 6.41%), protein (1.15 to 2.13%), total sugar (0.05 to 0.56%) and lipids (0.40 to 1.24). These differences can be due the variations of raw material and process. The paste properties of flours were different, with viscosity peak ranged from 73.33 to 387.08 RVU, breakdown (2.42 to 248.83RVU, final viscosity (154.92 to 275.5 RVU) and retrogradation tendency (50.75 to 132.5 RVU), showing the influence of kind of processing on flour viscosity.

Composição físico-química e cor de clones de mandioquinha-salsa

This study aimed to characterize the physical and chemical composition of ten items of arracacha grown in the municipality of São Manuel for the 2009 harvest. In the roots of the clones BGH (4560, 5741, 5744, 5746, 5747, 6414, 6513, 6525 and 7609) and the cultivar Amarela de Senador Amaral the characteristics evaluated were: color (L *, a * and b *) and moisture, ash, crude fiber, raw grease, protein, reducing sugars, total sugars and starch. After obtaining the data, an analysis was performed for the variance of test F and comparisons between the means made by the Tukey test at 5% probability. There was no significant difference to the results of luminosity (L *) while BGH 6414 and BGH 5744 showed the highest values for chroma and * BGH 5741, BGH 6414, BGH 7609, 'Amarela de Senador Amaral' BGH 5747 presented the highest chroma values for b *. Clones BGH 7609 and BGH 6414 showed significantly higher levels of dry matter and with the potential yield of agro-industrial processes it would be best suited in the form of frying. The materials that showed significantly larger amounts of ash were BGH 6525, BGH 5747, 'Amarela de Senador Amaral ", BGH 4560, BGH 5746, BGH 6513. Regarding the contents of fatty matter BGH 6525, BGH 5741 and BGH 5744 showed the highest levels. The results of BGH 7609 showed crude fiber significantly higher than the other materials tested, it can be used in diets composed of fibers. BGH 4560 and cultivar had the highest crude protein. BGH 5741 showed the lowest reducing sugar content among the clones, but not significantly different from results found for the cultivar. All clones showed total sugar levels were higher in the cultivar, which may have more flavor. BGH 5741, BGH 5746, BGH 6525 and BGH 6513 showed significantly higher starch content than the cultivar Amarela de Senador Amaral. From these results we conclude that the clones have similar color characteristics, and are potentially a nutritionally adequate substitute for the cultivar.

Alterações nuricionais em casca e polpa de cenoura decorrentes de diferentes métodos de cozimento

The aim of this study was to verify the carrot cooking most suitable method to minimize nutrient losses. Carrot peel slices were subjected to pre cooking tests that were initiated with 0.5 min of duration and then increased in 0.5 min successively. The carrot pieces texture was monitored during the pre tests so all would havethe same texture independent of the type of cooking. The degree of softennes was evaluated by pressuring the pieces between the toes. The carrot pulp and pell were subjected to four types of heat treatment (pressure, immersion, microwave, and steam), after that they were pounded with a food processor and stored at -18 ºC. The nutritional analyses were as follow: The evalu determination of proteins, lipids, fibers, sugars reducers, total of ascorbic acid content and minerals (iron, calcium, zinc, magnesium, potassium, phosphorus, and calcium). The analyses were accomplished with fresh carrot and after cooking with the different methods. The peel of the carrot presented as amounts of proteins, lipids, fibers percentages, sugars reducers, total and ascorbic acid content equivalent to the pulp. In addition, the minerals content was superior in the peel in relation to the pulp, presenting respective percentages of 38,10%, 95,12%, 47,04%, 58,88%, 70,27% and 21,27%. There were nutrient losses in relation to the raw vegetable, when the carrot pieces were submitted to the different cooking methods. The methods of steaming and microwave had lower nutritional losses.