Notes et extraits de Pierre-François CHIFFLET, concernant principalement la Franche-Comté et la Bourgogne.

On remarque : « Ex Glossario pervetusto » (f. 3) ; — « Ex chartis ecclesiae de Caritate » (f. 10) ; — « Ex veteri membrana et ex antiquo circulo depicto propè altare Maurimonasterii in Alsatia » (f. 10) ; — « Ex chartis R. P. Stephani Voyrin » (f. 11) ; — « Episcopi Metenses, ex ms. codice Sancti Arnulfi Metensis » (f. 12 v°) ; — Lettre de Hugues Métel à Gerland (f. 14) ; — « Ex variis catalogis sanctorum » (f. 14) ; — « Ex archivio ecclesiae Beatae Magdalenae [Bisuntinae] » (f. 17) ; — « Ex archivio Sancti Vincentii Bisont[ini] » (f. 18) ; — « Ex archivio monasterii Bellaevallis » [Bisuntinae dioecesis] (f. 20) ; — « Ex veteri necrologio Sancti Stephani [Bisuntini], quod est ad margines Martyrologii Bedae perantiqui, ad ejusdem ecclesiae usum accommodati » [cf. ms. de Besançon 712] (f. 23 v°) ; — « Inter martyrologium et regulam, seu librum primum concilii Aquisgranensis, habetur haec sententia absolutionis » (f. 25) ; — « Ante veterem Ordinem Romanum, qui est Liber Pontificalis, habentur sequentes formulae praestandae obedientiae archiepiscopis et ecclesiae Bisuntinae » (f. 25 v°) ; — « Alia ex archivo archiepiscopi [Bisuntini] » (f. 26 v°) ; — « Ex archivio Balernensi » (f. 27 v°) ; — « Ex variis sanctorum historiis manuscriptis » (f. 30) ; — « Extraits concernant Tournus » (f. 34 v°), — notamment : « Ex codice manuscripto Trenorchiensi, membraneo, quod vocare possis Registrum Trenorchiense, incipiens ab anno 1222 et desinens in 1296 ; inscribitur : Feoda ecclesiae Trenorchiensis » (f. 37) ; — « Ex notis R. P. Voyrini » [cf. supra, f. 11] (f. 49) ; — « Inscriptio vetus Luxoviensis » (f. 50 v°) ; — Notes historiques, concernant principalement des abbayes comtoises (f. 51) ; — « Scriptores rerum Burgundicarum » (f. 52) ; — « Ex parvo chartulari Sancti Benigni [Divionensis] » (f. 52 v°) ; — « Ex libro anniversariorum Sancti Benigni [Divionensis] », 1579-1629 (f. 52 v°) ; — « Ex chartulari Sancti Symphoriani Augustodunensis » (f. 53) ; — « Ex chartulari Sancti Marcelli Cabilonensis » (f. 53 v°) ; — « Ex chartulari S. Sequani » [Lingonensis dioecesis] (f. 54 v°) ; — « Ex chartulari Sanctae Capellae Divionensis » (f. 55) ; — « Ex chartulari cathedralis Augustodunensis » (f. 55 v°) ; — « Ex tomo priore chartularis Sancti Stephani Divionensis » (f. 56) ; — « Ex chartulari Cluniacensi » (f. 57) ; — « Ex apographo chartularis Matisconensis, cujus autographum vetus dicebatur Liber catenatus » (f. 57 v°) ; — « Ex chartulari Patriciaci, qui est prioratus dependens a Floriacensi abbatia, situs in comitatu Kadrellensi, dioecesi Augustodunensi » (f. 58 v°) ; — Extraits concernant Autun et les comtes d'Autun (f. 60 v°) ; — « Ex autographis Sancti Benigni Divionensis » (f. 61) ; — « Iterum ex chartulari Sancti Stephani Divionensis » (f. 61) ; — « Ex autographis monasterii puellarum Tartensis » (f. 62) ; — « Iterum ex tabulario Cluniacensi, adnotatis paginis » (f. 62 v°) ; — « Ex majore tabulario ecclesiae Cabilonensis, quod digessit Johannes Germani, Cabilonensis episcopus » (f. 64 v°) ; — « Ex tabulario abbatiae de Buxeria » (f. 65) ; — « Ex autographis monialium Benedictinarum de Pralon » (f. 66) ; — « Ex autographis prioratus Vallis Beatae Mariae juxta Talant, de Ordine Vallis Scholarium, vulgò Bonvau » (f. 66) ; — « Ex kalendario pervetusto Sancti Benigni Divionensis » (f. 66 v°) ; — « Ex martyrologio proprio Sancti Martini Eduensis » (f. 67) ; — « Ex necrologio Sancti Lazari Aeduensis » (f. 67) ; — « Ex chronico Sancti Petri Vivi Senonensis » (f. 67) ; — « Ex chronico S. Medardi Suessionensis » (f. 68) ; — « Ex brevi chronico Sancti Dionysii ad cyclos paschales » (f. 68) ; — « De Saracenis in Gallia profligatis » (f. 69) ; — « Ex necrologio Sancti Augendi Jurensis » (f. 74) ; — « Ex necrologio Sancti Petri Bisuntini » (f. 74) ; — « Ex necrologio veteri Sancti Stephani [Bisuntini] » (f. 74 v°) ; — « Ex registro Innocentii papae VI, anni IX pontificatus, Christi 1361 » (f. 82) ; — « Ex tabulis capituli Sancti Dionysii de Vergeyo, nunc Nuciacensis » (f. 83) ; — « Ex manuscripto codice donationum 208 Joannis, comitis Burgundiae et domini Salinensis, super puteum Salinarum » (f. 89 v°) ; — « Ex archivo Sancti Anatolii Salinensis ; ex magno necrologio chartaceo eique simili membraneo, composito 1390 » (f. 92) ; — « Ex chartis prioratus de Marthereto prope Vesulium » (f. 94) ; — « Ex archivo Sancti Pauli Bisonticensis » (f. 95) ; — « Ex veteri chartulario Sancti Pauli [Bisonticensis] » (f. 95 v°) ; — « Ex veteri manuscripto codice Sancti Pauli [Bisonticensis] » (f. 96) ; — « Ex antiquioribus libris ecclesiae Sancti Stephani [Bisonticensis], scriptis aut in usum adhibitis tempore Hugonis primi, [archiepiscopi Bisonticensis] » (f. 96 v°) ; — « Ex codice Sancti Stephani [Bisonticensis] pervetusto, qui Bisonticensis ecclesiae sacros ritus continet » (f. 97) ; — « Ex antiquis codicibus Sancti Pauli [Bisonticensis] » (f. 98) ; — « Ex antiquo rituum libro » (f. 98) ; — « Ex antiquis letaniis, tempore Hugonis I, [archiepiscopi Bisuntini], in ecclesia Bisontina cani solitis ; ex manuscriptis Sancti Stephani et Sancti Pauli » (f. 99) ; — « Extraits de plusieurs manuscrits de Saint-Paul de Besançon » (f. 99 v°) ; — « Ex manuscripto codice Sanctae Magdalenae [Bisuntinae] » (f. 101) ; — Inscription trouvée à Saint-Ferjeux, en 1627 (f. 101 v°) ; — « Ex antiquis membranis archivii ecclesiae metropolitanae Bisontinae », extraits de bulles pontificales, etc. (f. 102) ; — « Ex archivio Loci Crescentis seu Trium Regum » (f. 105) ; — Notes empruntées, « ut videtur », à Anselme « de Marenchiis » (f. 106) ; — « Ex actis capituli Bisontini » (f. 106 v°) ; — « Ex obitibus adnotatis ad marginem martyrologii cathedralis Aeduensis » (f. 106 v°) ; — Fragment de chronique, 563-1033, tiré « ex veteri membrana monasterii Sancti Augendi » (f. 107) ; — « Ex alia membrana, post chronicon Engolismense » (f. 107) ; — « Ex archivio abbatiae de Aceyo » (f. 108) ; — « Ex archivio abbatiae de Roseriis » (f. 109) ; — « Extrait d'un tome des recognoissances des fiefs du Charolois » (f. 109 v°) ; — « Ex polypthico Sancti Vincentii Cabilonensis » (f. 114) ; — « Ex Aeneae, Parisiensis episcopi, collectione contra Graecos, Biblioth. Thuan., n. 444 » (f. 114) ; — « Consuetudines Cluniacenses, auctore Wdalrico, monacho Cluniacensi » (f. 115) ; — Inscriptions copiées « in pervetusto manuscripto codice, post Persii satyras », etc. (f. 116) ; — Extraits d'une « Vita metrica sancti Eustachii martyris, cognomine Placidae », contenue « in manuscripto codice Cisterciensi », etc. (f. 116 v°) ; — « De archiepiscopis Bisontinis » (f. 119) ; — « Ex archivio capituli Bisontini » (f. 120 v°) ; — « Notitia nostrorum diplomatum » (f. 124-135 r° , 141 r° , 142 v° et 143 r°) ; — « De comitibus Burgundiae in Sancti Stephani basilica Bisontina sepultis, ex veteri membrana archivi ecclesiae metropolitanae » (f. 135 v°) ; — Lettre autogr. de « Pierre-François Chifflet » [à Baluze], Dijon, 18 mars 1657 (f. 161) ; — Fragment de charte, « extrait du thresor de S. Anatoile de Salins » (f. 162) ; — Lettre autogr. de « [fr.] François Du Chemin » à Chifflet, Cîteaux, 16 mai 1657 (f. 167) ; — « Nova regum Francorum series, a quâ Theodericus II et omnes ejus posteri excluduntur, ex manuscripto codice Fontanetensi » (f. 171) ; — « Vita sancti Theutbaldi, Viennensis archiepiscopi », incomplète [Bibliotheca hagiographica latina, 8044 b] (f. 172 et 172 bis) ; — « Ex chartulario Sancti Germani Autisiodorensis », diplômes carolingiens (f. 174) ; — Fragment d'une lettre [adressée vraisemblablement à Chifflet], Paris, octobre 1670 (f. 177) ; — Extraits « ex vitâ sancti Theutbaldi, Viennensis archiepiscopi..., ex manuscripto codice monasterii Sancti Theuderii, ubi sanctus Theobaldus, Viennensis archiepiscopus, requiescit » [extraits de la vie dont un fragment occupe les feuillets 172 et 172 bis] (f. 178) ; — Lettre autogr. de « P[ierre-] F[rançois] Chifflet » [à Baluze], 10 mars (f. 180) ; — Notes sur saint Théobald, archevêque de Vienne (f. 181). Cf. Ulysse Robert, Catalogue des manuscrits relatifs à la Franche-Comté, qui sont conservés dans les bibliothèques publiques de Paris (1878), p. 161-162.

Erosão na cultura do milho em sucessão à aveia preta e pousio descoberto, em preparo convencional e plantio direto, com tração animal e tratorizada

Empregando chuva simulada em Argissolo Vermelho distrófico arênico, com declividade média de 0,12 m m-1, avaliou-se a erosão na cultura do milho (Zea mays L.) em preparo convencional e plantio direto, utilizando tração animal e tratorizada, antecedida de pousio descoberto e aveia preta (Avena strigosa Schieb) no inverno, no período compreendido entre 1994 e 1995. Chuvas simuladas na intensidade de 64 mm h-1 e duração de 60 a 105 min foram aplicadas em quatro épocas: logo após a semeadura, 30 e 60 dias após a emergência e logo após a colheita do milho. A cobertura do solo propiciada pela cultura de inverno foi reduzida em 90% após o preparo convencional, independentemente da forma de tração. As perdas de solo e água medidas durante o desenvolvimento do milho foram sempre maiores sob preparo convencional do que sob plantio direto, independentemente da forma de tração, exceto logo após a colheita, quando a resteva estava uniformemente distribuída na superfície do solo. No preparo convencional, a erosão na cultura do milho foi, na média das formas de tração, 45% menor, quando antecedida de aveia preta no inverno do que quando antecedida de pousio descoberto. Por sua vez, a cultura do milho, por si só, reduziu a erosão em 60%, na média das formas de tração, comparada à do solo descoberto. A forma de tração influenciou a erosão somente no preparo convencional, com a animal apresentando perdas totais de solo 55 e 15% inferiores à tratorizada, respectivamente na cultura do milho e em solo descoberto. A perda de água por enxurrada foi mais afetada pela forma de tração no preparo convencional, totalizando, em média, 44% na animal e 57% na tratorizada, em relação ao volume total de chuva aplicado.

Extraits relatifs à l'HISTOIRE ECCLESIASTIQUE DE LA FRANCE.

Contient : Colb. 829 [lat. 4114] : érection des évêchés d'Alet et de Saint-Pons de Thomières ; Colb. 842 [lat. 8569] : lettres de Pétrarque ; Colb. 1020 [lat. 5239] : Chroniques de Limoges ; Colb. 1223 [lat. 4893] : chronique dite de Guillaume Godel ; Colb. 1150 [lat. 5247] : nécrologe de Saint-Robert de Cornillon [Molinier, Obituaires, n° 643] ; Colb. 1248 [lat. 4833 A] : bulle de Jean V pour Gui de La Tour [Jaffé, n° 4107] ; Colb. 1297 [lat. 2627] : Vita sancti Bibiani ; Colb. 1340 [lat. 5064] : notes sur le concile de Latran de 1215 ; Colb. 1297 [lat. 2627] : lettre d'Etienne, abbé de Cluny, aux moines de Moissac ; Colb. 1359 [lat. 5178] : lettre adressée à l'évêque de Ratisbonne au sujet d'une nouvelle hérésie (1466) ; Colb. 1364 [lat. 2294] : catalogue de reliques ; Colb. 1392 [lat. 4042] : lettre de l'évêque de Laon à Boniface VIII ; Colb. 1393 [lat. 1461] : notes et lettres diverses sur l'histoire de l'Église au début du XIVe siècle ; Colb. 1480 [lat. 3127] : lettres historiques du XVe siècle ; Lettre de Nicolas Goizot, s. d., touchant la mort de D. Placide Porcheron (1694) ; Extraits de divers traités touchant le schisme

Documents et pièces diverses, en majeure partie originaux, conservés jadis dans le TRESOR DES CHARTES DE LORRAINE. — Nombreux sceaux. — Voy. l'analyse détaillée de la plupart de ces pièces dans l'Inventaire de Dufourny (Bibl. nat., ms. fr. 4880-4886 ; Bibl. de Nancy, mss. 754-765). On ne relève ici que les pièces qui n'y figurent pas. LXXXVIII — Portefeuille-layette 44 b (1403-1496)

Contient : Vidimus (1497) des lettres de Marguerite, duchesse de Bourgogne, portant réception de l'hommage de Ferry, comte de Vaudémont, pour une rente sur le tonlieu de Damme (Arras, 22 novembre 1404) ; Affranchissement accordé par Marguerite de Joinville, comtesse de Vaudémont, à Geoffroy Parmentier et à Jeannette, sa femme, demeurant à Vézelise (1415) ; Lettres d'Antoine de Lorraine au duc de Bar, au sujet de déprédations commises dans la terre de Montiers-sur-Saulx (Vézelise, 1430) ; Déclaration de Philippe le Bon, duc de Bourgogne, concernant la mise en liberté de prisonniers faits depuis la trève, conclue sur sa médiation entre le duc de Bar et le comte de Vaudémont (1432) ; Lettres de Charles VII au comte Antoine (1443) ; Lettres du roi René au même (1435-1437) ; Lettres de la duchesse Isabelle au même (1435) ; « Instrument de Joffroi Bouquin, pour un accort fait à Condet-sur-Muselle, le xxije jour d'aoust l'an lxij, entre Pierre de Moncelz et le dit Joffroi pour ma dame, et messire Jehan de Saulmez pour monseigneur le duc de Bar » (28 mars 1363 ; n° 91 de la layette Traités III) ; Lettres du roi René portant don à Ferry II, comte de Vaudémont, du montant de l'imposition de 16 fl. par feu dans les seigneuries possédées par le dit Ferry en Provence (Angers, 27 décembre 1467) ; Vidimus (1477) des lettres du roi René, concédant la seigneurie de Beaufrémont au comte de Vaudémont (Marseille, 13 avril 1470) ; Mandement du roi René pour le paiement des gages de René, comte de Vaudémont, nommé gouverneur d'Anjou (Aix, 7 février 1473) ; Instructions données à Jean de Saint-Amadour et Huyn Roynette, lieutenant de Nancy, pour une mission auprès du roi (XVe siècle)

Soft tissue sarcomas of the trunk wall (STS-TW): a study of 343 patients from the French Sarcoma Group (FSG) database.

BACKGROUND: Soft tissue sarcomas of the trunk wall (STS-TW) are usually studied together with soft tissue sarcomas of other locations. We report a study on STS-TW forming part of the French Sarcoma Group database. PATIENTS AND METHODS: Three hundred and forty-three adults were included. We carried out univariate and multivariate analysis for overall survival (OS), metastasis-free survival (MFS) and local recurrence-free survival (LRFS). RESULTS: Tumor locations were as follows: thoracic wall, 82.5%; abdominal wall, 12.3% and pelvic wall, 5.2%. Median tumor size was 6.0 cm. The most frequent tumor types were unclassified sarcoma (27.7%) and myogenic sarcoma (19.2%). A total of 44.6% of cases were grade 3. In all, 21.9% of patients had a previous medical history of radiotherapy (PHR). Median follow-up was 7.6 years. The 5-year OS, MFS and LRFS rates were 60.4%, 68.9% and 58.4%, respectively. Multivariate analysis retained PHR and grade for predicting LRFS and PHR, size and grade as prognostic factors of MFS. Factors influencing OS were age, size, PHR, depth, grade and surgical margins. The predictive factors of incomplete response were PHR, size and T3. CONCLUSIONS: Our results suggest similar classical prognostic factors as compared with sarcomas of other locations. However, a separate analysis of STS-TW revealed a significant poor prognosis subgroup of patients with PHR.

A Randomized Controlled Trial Comparing Intradermal vs. Intramuscular Influenza Vaccine in Lung Transplant Recipients

Background: Immunogenicity of standard infl uenza vaccine is suboptimal in lung transplant recipients. Intradermal vaccine may elicit stronger responses due to recruitment of local dendritic cells. We compared the immunogenicity of the infl uenza vaccine administered intradermally (ID) to the standard intramuscular (IM) vaccination. Methods: In this investigator-blinded, two-center, prospective trial, lung transplant patients were randomized to receive intradermal (6ug) or intramuscular (15ug) 2008/9 trivalent inactivated infl uenza vaccine. Immunogenicity was evaluated using a standard hemagglutination inhibition assay (HIA). Response to the vaccine was defi ned as a fourfold increase of the HIA levels for any of the 3 viral strains in the vaccine. Geometric mean titers (GMT) and seroprotection rate (HIA ≥32) were also analyzed. Patients were followed during 6 months for the development of infl uenza or acute rejection. Results: We randomized 84 patients to receive the ID (n=41) vs. IM (n=43) vaccine, respectively. Baseline characteristics were similar between groups. Median time from transplantation was 3.4 yrs (ID) vs. 3.3 yrs (IM) (p=0.84). Vaccine response to at least one antigen was seen in 6/41 (14.6%) patients in the ID vs. 8/43 (18.6%) in the IM group (p=0.77). In the ID group, GMTs (95% CI) after vaccination were 15.7 (11.1-22.3) for H1N1, 84.0 (52.0-135.7) for H3N2, and 14.5 (9.6-21.8) for B strains vs. in the IM group 17.5 (11.8-25.9) for H1N1, 108.9 (77.5-153.2) for H3N2, and 20.2 (12.8-31.9) for B (p=NS, all 3 strains). Seroprotection was 39% (H1N1), 82.9% (H3N2) and 29.3% (B strain) in the ID group vs. 27.9% (H1N1), 97.7% (H3N2) and 58.1% (B strain) in the IM group. No factors associated with vaccine response were identifi ed. Mild adverse events were seen in 44% of patients (ID) vs. 34% (IM) (p=0.38). Two patients (4.8%) in the ID group developed infl uenza infection compared to none in the IM group. Two patients in each group developed biopsy-proven acute rejection during follow-up. Conclusions: Immunogenicity of the 2008/09 infl uenza vaccine was poor in lung transplant recipients. ID administration of the vaccine elicited similar immune responses to standard IM vaccination. Novel strategies of vaccination are needed to protect lung transplant recipients from infl uenza.

Integrated Analysis of High-Resolution Gene Expression and Copy Number Profiling Identified Biallelic Deletion of CDKN2A/2B Tumor Suppressor Locus As the Most Frequent and Unique Genomic Abnormality in Diffuse Large B-Cell Lymphoma (DLBCL) with Strong Prognostic Value in Both GCB and ABC Subtypes and Not Overcome by a Dose-Intensive Immunochemotherapy Regimen Plus Rituximab. Results of a Prospective GELA Clinical Trial Program

Background and aim of the study: Genomic gains and losses play a crucial role in the development and progression of DLBCL and are closely related to gene expression profiles (GEP), including the germinal center B-cell like (GCB) and activated B-cell like (ABC) cell of origin (COO) molecular signatures. To identify new oncogenes or tumor suppressor genes (TSG) involved in DLBCL pathogenesis and to determine their prognostic values, an integrated analysis of high-resolution gene expression and copy number profiling was performed. Patients and methods: Two hundred and eight adult patients with de novo CD20+ DLBCL enrolled in the prospective multicentric randomized LNH-03 GELA trials (LNH03-1B, -2B, -3B, 39B, -5B, -6B, -7B) with available frozen tumour samples, centralized reviewing and adequate DNA/RNA quality were selected. 116 patients were treated by Rituximab(R)-CHOP/R-miniCHOP and 92 patients were treated by the high dose (R)-ACVBP regimen dedicated to patients younger than 60 years (y) in frontline. Tumour samples were simultaneously analysed by high resolution comparative genomic hybridization (CGH, Agilent, 144K) and gene expression arrays (Affymetrix, U133+2). Minimal common regions (MCR), as defined by segments that affect the same chromosomal region in different cases, were delineated. Gene expression and MCR data sets were merged using Gene expression and dosage integrator algorithm (GEDI, Lenz et al. PNAS 2008) to identify new potential driver genes. Results: A total of 1363 recurrent (defined by a penetrance > 5%) MCRs within the DLBCL data set, ranging in size from 386 bp, affecting a single gene, to more than 24 Mb were identified by CGH. Of these MCRs, 756 (55%) showed a significant association with gene expression: 396 (59%) gains, 354 (52%) single-copy deletions, and 6 (67%) homozygous deletions. By this integrated approach, in addition to previously reported genes (CDKN2A/2B, PTEN, DLEU2, TNFAIP3, B2M, CD58, TNFRSF14, FOXP1, REL...), several genes targeted by gene copy abnormalities with a dosage effect and potential physiopathological impact were identified, including genes with TSG activity involved in cell cycle (HACE1, CDKN2C) immune response (CD68, CD177, CD70, TNFSF9, IRAK2), DNA integrity (XRCC2, BRCA1, NCOR1, NF1, FHIT) or oncogenic functions (CD79b, PTPRT, MALT1, AUTS2, MCL1, PTTG1...) with distinct distribution according to COO signature. The CDKN2A/2B tumor suppressor locus (9p21) was deleted homozygously in 27% of cases and hemizygously in 9% of cases. Biallelic loss was observed in 49% of ABC DLBCL and in 10% of GCB DLBCL. This deletion was strongly correlated to age and associated to a limited number of additional genetic abnormalities including trisomy 3, 18 and short gains/losses of Chr. 1, 2, 19 regions (FDR < 0.01), allowing to identify genes that may have synergistic effects with CDKN2A/2B inactivation. With a median follow-up of 42.9 months, only CDKN2A/2B biallelic deletion strongly correlates (FDR p.value < 0.01) to a poor outcome in the entire cohort (4y PFS = 44% [32-61] respectively vs. 74% [66-82] for patients in germline configuration; 4y OS = 53% [39-72] vs 83% [76-90]). In a Cox proportional hazard prediction of the PFS, CDKN2A/2B deletion remains predictive (HR = 1.9 [1.1-3.2], p = 0.02) when combined with IPI (HR = 2.4 [1.4-4.1], p = 0.001) and GCB status (HR = 1.3 [0.8-2.3], p = 0.31). This difference remains predictive in the subgroup of patients treated by R-CHOP (4y PFS = 43% [29-63] vs. 66% [55-78], p=0.02), in patients treated by R-ACVBP (4y PFS = 49% [28-84] vs. 83% [74-92], p=0.003), and in GCB (4y PFS = 50% [27-93] vs. 81% [73-90], p=0.02), or ABC/unclassified (5y PFS = 42% [28-61] vs. 67% [55-82] p = 0.009) molecular subtypes (Figure 1). Conclusion: We report for the first time an integrated genetic analysis of a large cohort of DLBCL patients included in a prospective multicentric clinical trial program allowing identifying new potential driver genes with pathogenic impact. However CDKN2A/2B deletion constitutes the strongest and unique prognostic factor of chemoresistance to R-CHOP, regardless the COO signature, which is not overcome by a more intensified immunochemotherapy. Patients displaying this frequent genomic abnormality warrant new and dedicated therapeutic approaches.

Adenoid Cystic Carcinoma of the Breast: A Rare Cancer Network Study

Purpose/Objective(s): Mammary adenoid cystic carcinoma (ACC) is a rare breast cancer variant. It accounts for less than 0.1% of all invasive breast malignancies. Typically, it presents as a small breast lump with a low propensity to metastasize to regional lymph nodes or distant sites. The aim of this retrospective multicenter Rare Cancer Network study is to assess prognostic factors and patterns of failure in ACC, as well as the role of radiation therapy (RT) in this rare disease. Materials/Methods: Between January 1980 and December 2007, 61 women with breast ACC were included in this study. Median age was 59 years (range, 28-94 years). The majority of the patients had good performance status (49 patients with WHO 0, 12 patients with WHO 1), and 70% of the patients (n = 42) were premenopausal. Surgery consisted of tumorectomy in 35 patients, mastectomy in 20, or quadrantectomy in 6. Median tumor size was 20 mm (range, 6-170 mm). Surgical margins were clear in 50 (82%) patients. Axillary dissection (n = 41) or sentinel node assessment (n = 10) was realized in the majority of the patients. There were 53 (87%) pN0 and 8 pNx (13%) patients. Estrogen (ER) and progesterone receptor (PR) was negative in 43 (71%) and 42 (69%) patients, respectively. In 16 patients (26%), the receptor status was unknown. Adjuvant chemotherapy or hormonotherapy was administered in 8 (13%) and 7 (12%) patients, respectively. Postoperative RT with a median total dose of 50 Gy (1.8-2.0 Gy/fraction; range, 44-70 Gy) was given in 40 patients. Results: With a median follow-up of 79 months (range, 6-285 months), 5-year overall and disease-free survival (DFS) rates were 94% (95% confidence interval [CI]: 88-100%) and 82% (95% CI: 71-93%), respectively. Five-year locoregional control rate was 95% (95% CI: 89-100%). There were only 4 patients with local relapse who were all salvaged successfully, and 4 other patients developed distant metastases. According to the Common Terminology Criteria for Adverse Events v3.0, late toxicity consisted of grade 2-3 cutaneous fibrosis in 4 (10%) patients, grade 1-2 edema in 2 (5%), and grade 3 lung fibrosis in 2 (5%). In univariate analyses, the outcome was influenced neither by the type of surgery nor the use of postoperative RT. However, positive receptor status had a negative influence on the outcome. Multivariate analysis (Cox model) revealed that negative ER (p = 0.006) or PR (p = 0.04) status was associated with improved DFS. Conclusions: ACC of the breast is a relatively indolent disease with excellent local control and survival. The prognosis of patients with ACC is much better than that for patients with other breast cancers, especially those who are ER and PR negative. The role of postoperative RT is not clear. More aggressive treatments may be warranted for patients with positive receptor status.

Erosividade, coeficiente de chuva, padrões e período de retorno das chuvas de Quaraí, RS

O conhecimento da potencialidade das chuvas em causar erosão é necessário para planejamento de atividades agrícolas e de engenharia civil. Para a localidade de Quaraí (RS), foram determinados a erosividade da chuva e a relação com a precipitação e o coeficiente de chuva, os padrões hidrológicos e o período de retorno das chuvas. Utilizaram-se dados pluviográficos diários do período 1966-2003. Para cada chuva erosiva, foram separados os segmentos do pluviograma com a mesma intensidade e registrados os dados em planilha. Com o programa Chuveros, foram calculadas as erosividades mensal, anual e média das chuvas pelo índice EI30, no Sistema Internacional de Unidades, e os padrões hidrológicos de chuva, bem como o coeficiente de chuva. Foram realizadas correlações de Pearson e regressões lineares simples entre o índice de erosividade EI30 e os valores médios mensais (p) e anuais (P) de precipitação e do coeficiente de chuva (Rc). Foi calculada a intensidade máxima da chuva pelo método da distribuição extrema tipo 1 para durações de chuva de 1/6, 1/3, 1/2, 1, 2, 4, 8, 12, 24 e 48 h e períodos de retorno da chuva de 2, 5, 10, 20, 50 e 100 anos. Foram ajustadas equações que relacionam a intensidade máxima e a duração da chuva para os períodos de retorno da chuva de 2, 5, 10, 20, 50 e 100 anos, pelo método de regressão linear simples, e construído o gráfico que relaciona essas características da chuva. O valor médio anual de EI30 (fator R da USLE) calculado para Quaraí foi de 9.292 MJ mm ha-1 h-1 ano-1. Obtiveram-se as equações EI30 = -754,37 + 13,50 p (r² = 0,85) e EI30 = -47,35 + 82,72 Rc (R² = 0,84). Em relação ao total das chuvas estudadas, 44 % do número e 90 % do volume foram erosivas. Do número total das chuvas erosivas, 51 % foram do padrão hidrológico avançado, 25 % do intermediário e 24 % do atrasado, ao passo que, do volume total das chuvas erosivas, 57 % foram do padrão avançado, 25 % do intermediário e 18 % do atrasado. Das chuvas erosivas, 57 % da erosividade correspondeu a chuvas do padrão avançado, 25 % a chuvas do padrão intermediário e 18 % a chuvas do padrão atrasado.

Caracterização química e mineralógica de agregados de diferentes classes de tamanho de Latossolos Bruno e Vermelho localizados no estado do Paraná

O teor e a forma dos minerais da fração argila são determinantes na definição da morfologia dos agregados do solo. Objetivando estudar a mineralogia da fração argila e as propriedades químicas de diferentes classes de agregados de Latossolos (Latossolo Bruno Ácrico húmico - LBd e Latossolo Vermelho Distroférrico húmico - LVdf) originados de rochas basálticas no Estado do Paraná, coletaram-se amostras indeformadas em diferentes profundidades (horizontes Bw1 e Bw2) em perfis de solos localizados em duas toposseqüências (quatro perfis no LBd e três no LVdf). Após secagem e separação das amostras indeformadas em seis classes de agregados (2-4; 1-2; 0,5-1; 0,25-0,5; 0,105-0,25; < 0,105 mm) determinaram-se os teores de Si solúvel em ácido acético 0,5 mol L-1 e de K, Ca, Mg e Al trocáveis. A fração argila das diferentes classes de agregados também foi estudada por diferentes técnicas: difratometria de raios X, análise térmica e análises químicas. Verificou-se homogeneidade nos teores trocáveis de elementos entre as classes de tamanho de agregados dos horizontes Bw1 e Bw2 dos perfis dos Latossolos Bruno e Vermelho. A intensa e contínua pedogênese dos Latossolos não foi suficiente para homogeneizar a mineralogia da fração argila (característica mais estável que a dos teores trocáveis) dos agregados. A maior variação nos teores de minerais, em função da classe de tamanho dos agregados, foi para o perfil localizado na posição mais alta da toposseqüência do LBd: variação de 35 % nos teores de gibbsita no horizonte Bw1 e 44 % nos teores de caulinita no horizonte Bw2. Considerando, ainda, o efeito da classe de tamanho dos agregados sobre a mineralogia dos horizontes Bw1 e Bw2 do LBd e LVdf, verificaram-se variações em algumas características cristalográficos da goethita e hematita - diâmetro médio do cristal (DMC) e intensidade de substituição isomórfica de Fe por Al - e da caulinita (DMC).

Outcome and Prognostic Factors in Adenosquamous Carcinoma of the Head and Neck: A Multicenter Rare Cancer Network Study

Purpose/Objective(s): Adenosquamous carcinoma (AC) of the head and neck is a distinct entity first described in 1968. Its natural history is more aggressive than squamous cell carcinoma but this is based on very small series reported in the literature. The goal of this study was to assess the clinical profile, outcome, patterns of failure and prognostic factors in patients with AC of the head and neck treated by radiation therapy (RT) with or without chemotherapy (CT).Materials/Methods: Data from 18 patients with Stage I (n = 3), II (n = 1), III (n = 4), or IVa (n = 10) AC, treated between 1989 and 2009, were collected in a retrospective multicenter Rare Cancer Network study. Median age was 60 years (range, 48 - 73 years). Fourteen patients were male and 4 female. Risk factors, including perineural invasion, lymphangitis, vascular invasion, positive margins, were present in 83% of the patients. Tumor sites included oral cavity in 4, oropharynx in 4, hypopharynx in2, larynx in 2, salivary glands in 2, nasal vestibule in 2, nasopharynx in 1, and maxillary sinus in 1 patient. Surgery (S) was performed in all but 5 patients. S alone was performed in only 1 patient, and definitive RT alone in 3 patients. Fourteen patients received combined modality treatment (S+RT in 10, RT+CT in 2, and all of the three modalities in 2 patients). Median RT dose to the primary and to the nodes was 66 Gy (range, 50 - 72 Gy) and 53 Gy (range, 44 - 66 Gy), respectively (1.8 - 2.0 Gy/fr., 5 fr./ week). In 4 patients, the planning treatment volume included the primary tumor site only. Seven patients were treated with 2D RT, 7 with 3D conformal RT, and 2 with intensity-modulated RT.Results: After a median follow-up period of 38 months (range, 9 - 62 months), 8 patients developed distant metastases (lung, bone, mediastinum, and liver), 6 presented nodal recurrences, and only 4 had a local relapse at the primary site (all in-field recurrences). At last follow-up, 6 patients were alive without disease, 1 alive with disease, 9 died from progressive disease, and 2 died from intercurrent disease. The 3-year and median overall survival, disease-free survival (DFS) and locoregional control rates were 52% (95% confidence interval [CI]: 28 - 76%) and 39 months, 36% (95% CI: 13 - 49%) and 12 months, and 54% (95% CI: 26 - 82%) and 40 months, respectively. In multivariate analysis (Cox model), DFS was negatively influenced by the presence of extracapsular extension (p = 0.02) and advanced stage (IV versus I-III, p = 0.003).Conclusions: Overall prognosis of locoregionally advanced AC remains poor, and distant metastases and nodal relapse occur in almost half of the cases. However, local control is relatively good, and early stage AC patients had prolonged DFS when treated with combined modality treatment.

Mineralogical, K-Ar, stable and Sr isotope systematics of K-white micas during very low-grade metamorphism of limestones (Helvetic nappes, Western Switzerland)

Mineralogical, K-Ar, Rb-Sr and stable isotope analyses have been carried out on K-white micas from Helvetic Malm limestones in order to examine their evolution during very low- to low-grade Alpine metamorphism, associated with intense ductile deformation. Metamorphic temperatures were estimated al approximately 300-degrees-C from stable isotopes (quartz-calcite thermometry), occurrence of chloritoid, and `'epizonal'' illite crystallinity index. K-white micas consist of variable mixtures of 2M, phengite and muscovite, as revealed by detailed X-ray diffraction analyses using peak decomposition of the (060, 331) spectra. K-Ar apparent ages display a strong grain-size dependence in which mainly fine-grained size fractions (< 2 mum) record Alpine ages (37-15 Ma). However, these ages provide a relative rather than an absolute chronology of the diachronous Alpine metamorphic evolution of the Helvetic nappes. The resetting of the K-Ar isotopic system of K-white micas to Alpine metamorphic conditions reflects an apparent combination of crystallization/recrystallization and radiogenic Ar-40 diffusion loss. The oxygen isotope compositions of micas (+ 15 to + 22 parts per thousand) are intermediate between detrital and O-18-enriched values expected for micas neoformed within an abundant marine carbonate matrix. No isotopic equilibrium has been reached between calcite and micas. The variable depletion of hydrogen isotope compositions (- 126 to - 82 parts per thousand) is influenced by the interaction with organic matter under closed-system conditions. Organic matter, if not removed, may also represent a serious source of error in K-Ar age determination, by introducing radiogenic Ar-40 contamination. Sr-87/Sr-86 isotope ratios of micas range from 0.70879 to 0.70902 with one outlier at 0.71794. The low values reflect Sr exchange with calcite occurring during crystallization/recrystallization of micas under closed-system conditions.